Title:
ANTI-GPC3 ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2018/131586
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: an anti-GPC3 antibody that recognizes an epitope different from those recognized by existing antibodies (e.g., GC33 and GC199), and that can specifically bind to GPC3 localized on a cell membrane even as a single-chain antibody; a CAR including said anti-GPC3 single-chain antibody; an immunocompetent cell expressing said CAR; an anti-GPC3 antibody gene or CAR gene; a vector including said anti-GPC3 antibody gene or CAR gene; a host cell into which said vector has been introduced; a method for specifically detecting GPC3; and a kit for specifically detecting GPC3. This antibody includes the specific heavy-chain CDRs 1-3 and the specific light-chain CDRs 1-3 defined in claim 1, and specifically binds to a human-derived GPC3 polypeptide. This antibody specifically binds to GPC3 localized on a cell membrane. A CAR-immunocompetent cell prepared from a CAR including said single-chain antibody is useful in cancer immunotherapy.
Inventors:
TAMADA KOJI (JP)
SAKODA YUKIMI (JP)
NAKATSURA TETSUYA (JP)
SAITO KEIGO (JP)
SAKODA YUKIMI (JP)
NAKATSURA TETSUYA (JP)
SAITO KEIGO (JP)
Application Number:
PCT/JP2018/000257
Publication Date:
July 19, 2018
Filing Date:
January 10, 2018
Export Citation:
Assignee:
UNIV YAMAGUCHI (JP)
NAT CANCER CT (JP)
NAT CANCER CT (JP)
International Classes:
C12N15/09; C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; G01N33/574; C12P21/08
Domestic Patent References:
| WO2015097928A1 | 2015-07-02 | |||
| WO2004022739A1 | 2004-03-18 | |||
| WO2016049459A1 | 2016-03-31 | |||
| WO2013070468A1 | 2013-05-16 | |||
| WO2016036973A1 | 2016-03-10 | |||
| WO2016056228A1 | 2016-04-14 | |||
| WO2016056228A1 | 2016-04-14 |
Foreign References:
| JP2016523518A | 2016-08-12 | |||
| JP4011100B2 | 2007-11-21 | |||
| JPH02227075A | 1990-09-10 | |||
| JPH0322979A | 1991-01-31 | |||
| JPS59140885A | 1984-08-13 | |||
| JPS6070080A | 1985-04-20 | |||
| JPS60251887A | 1985-12-12 | |||
| JP2606856B2 | 1997-05-07 | |||
| JP2517813B2 | 1996-07-24 | |||
| JP2017001732A | 2017-01-05 |
Other References:
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HIPPO, Y. ET AL.: "Identification of soluble NH2-terminal fragment of glypican-3 as a serological marker for early-stage hepatocellular carcinoma", CANCER RESEARCH, vol. 64, no. 7, 1 April 2004 (2004-04-01), pages 2418 - 2423, XP002990186
NAKATSURA, T. ET AL.: "Era of cancer immunotherapy has come", JPN. J. CLIN. IMMUNOL., vol. 39, no. 3, January 2016 (2016-01-01), pages 164 - 171, XP055424198
KABAT, E. A. ET AL.: "Sequences of Proteins of Immunological Interest", 1991, US DEPT. HEALTH AND HUMAN SERVICES
CYTOTECHNOLOGY, vol. 3, no. 133, 1990, pages AS3 - 3
PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1987, pages 7413
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCLEIC ACID RES., vol. 12, 1984, pages 387
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 2444 - 2448
WIBURLIPMAN, SIAM J. APPL. MATH., vol. 44, 1984, pages 557 - 567
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
NATURE, vol. 329, 1987, pages 840
J. BIOCHEMISTRY, vol. 101, 1987, pages 1307
TAMADA K ET AL., CLIN CANCER RES, vol. 18, 2002, pages 6436 - 6445
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI. U.S.A, vol. 84, 1978, pages 1929
J. BACTERIOLOGY, vol. 153, no. 163, 1983
"Baculovirus Expression Vectors, A Laboratory Manual", 1992, W.H. FREEMAN AND COMPANY, article "Current Protocols in Molecular Biology"
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
J MOL BIOL., vol. 222, no. 3, 5 December 1991 (1991-12-05), pages 581 - 97
J MED VIROL., vol. 79, no. 6, June 2007 (2007-06-01), pages 852 - 62
PROC NATL ACAD SCI U S A., vol. 105, no. 20, 20 May 2008 (2008-05-20), pages 7287 - 92
JOURNAL OF VIROLOGY, vol. 78, no. 7, April 2004 (2004-04-01), pages 3325 - 3332
See also references of EP 3569709A4
HIPPO, Y. ET AL.: "Identification of soluble NH2-terminal fragment of glypican-3 as a serological marker for early-stage hepatocellular carcinoma", CANCER RESEARCH, vol. 64, no. 7, 1 April 2004 (2004-04-01), pages 2418 - 2423, XP002990186
NAKATSURA, T. ET AL.: "Era of cancer immunotherapy has come", JPN. J. CLIN. IMMUNOL., vol. 39, no. 3, January 2016 (2016-01-01), pages 164 - 171, XP055424198
KABAT, E. A. ET AL.: "Sequences of Proteins of Immunological Interest", 1991, US DEPT. HEALTH AND HUMAN SERVICES
CYTOTECHNOLOGY, vol. 3, no. 133, 1990, pages AS3 - 3
PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1987, pages 7413
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
DEVEREUX ET AL., NUCLEIC ACID RES., vol. 12, 1984, pages 387
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 2444 - 2448
WIBURLIPMAN, SIAM J. APPL. MATH., vol. 44, 1984, pages 557 - 567
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
NATURE, vol. 329, 1987, pages 840
J. BIOCHEMISTRY, vol. 101, 1987, pages 1307
TAMADA K ET AL., CLIN CANCER RES, vol. 18, 2002, pages 6436 - 6445
METHODS ENZYMOL., vol. 194, 1990, pages 182
PROC. NATL. ACAD. SCI. U.S.A, vol. 84, 1978, pages 1929
J. BACTERIOLOGY, vol. 153, no. 163, 1983
"Baculovirus Expression Vectors, A Laboratory Manual", 1992, W.H. FREEMAN AND COMPANY, article "Current Protocols in Molecular Biology"
BIO/TECHNOLOGY, vol. 6, 1988, pages 47
J MOL BIOL., vol. 222, no. 3, 5 December 1991 (1991-12-05), pages 581 - 97
J MED VIROL., vol. 79, no. 6, June 2007 (2007-06-01), pages 852 - 62
PROC NATL ACAD SCI U S A., vol. 105, no. 20, 20 May 2008 (2008-05-20), pages 7287 - 92
JOURNAL OF VIROLOGY, vol. 78, no. 7, April 2004 (2004-04-01), pages 3325 - 3332
See also references of EP 3569709A4
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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