| US3046285A | 1962-07-24 |
See also references of EP 0256095A4
| 1. | Carboxypregnane derivatives of the formula: wherein one X is COOR, C^COOR, CH(COOR)2, CONHR, CHjCONHR, or CN; remaining X's are H, F, CH3 , OH, COOR, CHjCOOR, CH(COOR)2 , CONHR, CH2CONHR, or CN; Y is R is H, alkyl of 15 carbon atoms, or benzyl; Rt is CH2OR3 , COOR, or CONHR; R2 is H, OR3 , or Br; R3 is H, CORt. , or tetrahydropyranyl; R[. is alkyl of 15 carbon atoms or benzyl; Rs is H or COR,. ; represents a single or double bond; « represents aposition, βposition or a mixture of α~ and βpositions; and represents αposition. |
| 2. | The derivatives of Claim. |
| 3. | of the formula: wherein X, Ri and R2 have the meanings given in Claim 1. |
| 4. | The derivatives of Claim 1 of the formula: wherein X, Rj , R2 , and R5 have the meanings given in Claim 1. |
| 5. | The derivatives of Claim 1 of the formula: wherein X has the meaning given in Claim 1. The derivatives of Claim 1 of the formula: wherein X has the meaning given in Claim 1. The derivatives of Claim 1 of the formula: wherein X X and Rj have the meanings given in Claim 1. |
| 6. | 7 The derivatives of Claim 1 of the formula: wherein Ri o Xj is H or F ; Rs is CH2OH, COOH, CONH2), CONHCH3 , COOCH3 , or CH2COOCH3 ; R7 is Hor COR13.; R8 is H or COCH3 ; R9 is Br, OH, or OCOCH3 ; R10 is Br, OH, or OCORj 3 ; Rn is CH(COOCH3)2, CH(COOH)2, CONH2 , CHCOOCH3 , CHCOOH, COOH, or CN; R12 is H or CH2COOR"ι3; and R13 is alkyl of 1. |
| 7. | carbon atoms or tetrahydropyranyl. |
| 8. | 8 The derivatives of Claim 6 of the formula: H2 COCORm t wherein Rl lt is alkyl of 15 carbon atoms ; and Rj 5 is OH or OCORi ι . |
| 9. | 9The derivatives of Claim 7 wherein Rj 4 is methyl and Ri 5 is OH . |
| 10. | 10 The process for preparing a pregnane derivative sub¬ stituted in the 6position with an alkyl ester group which comprises: (1) reacting cortisol or prednisolone with formalde¬ hyde and hydrogen chloride to produce (I) 17a, 20, 20, 21 bis(methylenedioxy)llβhydroxypregn4ene3one; (2) reacting (I) with ethylene glycol and pyridine hydrochloride to produce (II) 3 ,3ethylenedioxy17α, 20, 20, 21bis(methylenedioxy)pregn5enellβol; (3) epoxidizing (II) by treatment with mchloroperbenzoic acid to produce (III) 5, 6aepoxy3,3ethylenedioxy17a, 20,20, 21 bis(methylenedioxy) 5apregnanllβol; (4) reacting (III) with a solution an alkenylmagnesium halide of 2 carbon atoms to produce (IV) 3,3ethylenedioxy17 , 20,20,21bis(methylenedioxy) 6β (alkenyl) 5apregnane5llβdiol; (5) reacting (IV) with potassium permanganate and sodium periodate to produce (V) 3,3ethylenedioxyl7a,20,20, 1bis (methylenedioxy) 6βcarboxyaikyl5apregnane5, llβdiol; (6) esterifying the carboxyalkyl group by reacting (V) with an alkylating agent and recovering (VI) 3,3ethylenedioxy 17 , 20,20, 1bis(methylenedioxy) 6βalkoxycarbonylalkyl5a pregnane5li diol; (7) reacting (VI) with sulfuric acid to produce (VII) 17a, 20,20,21bis(methylenedioxy) 6βalkoxycarbonylalkyl5αpregnan3 one; and (8) reacting (VII) with activated magnesium silicate to produce (VIII) llβhydroxy6βalkoxycarbpnylalkyl17 ,20,20,21 bis(methylenedioxy) pregn4en3one. |
| 11. | 11 The process of Claim 9 which additionally comprises re¬ acting (VIII) with formic acid to produce llβ, 17a, 21trihydroxy 6βalkoxyσarbonylalkyl4pregnene , 20dione. |
| 12. | 12 The process of Claim 9 which additionally comprises re¬ acting (VIII) with 2,3dichloro5,6dicyanaquinone to produce (IX) llβhydroxy6βalkoxycarbonylalkyl17a, 20,20,21bis(methylenedioxy) pregn1, 4diene3one. |
| 13. | 13 The process of Claim 11 which additionally comprises re¬ acting (IX) w th formic acid to produce llβ, 17a, 21trihydroxy6β alkoxycarbonylaikyi1, 4pregnadien3, 20dione. |
| 14. | 14 The process for preparing a pregnane derivative substituted in the 16ρosition which comprises: (1) reacting prednisolone with triethylorthoacetate to produce (I) prednisolone17, 21ethylorthoacetate; (2) reacting (I) with sodium acetate buffer in methanol to produce (II) prednisolone 17acetate; (3) reacting (II) with acetic anhydride in pyridine to produce (III) prednisolone17, 21diacetate; (4) # reacting (III) with potassium acetate in dimethyl formamrde to produce (IV) 16, 17dehydroprednisolone21acetate; (5) saponifying (IV) to produce (V) 16, 17dehydropredni solone; (6) reacting (V) with 2, 3dihydropyran and pyridinium tosylate to produce (VI) 16, 17dehydroprednisolone21tetra hydropyran; and (7) reacting (VI) with a mixture of sodium methylate and dimethylmalonate to produce (VII) 2ltetrahydropyranyl16yl (dimethylmalonyl) llβhydroxy3 , 2ϋdιoxol,4ρregnadiene. |
| 15. | 15 The process of Claim 13 which additionally comprises re¬ acting (VII) with hydrogen chloride in methanol to produce prednisolone16dimethylmalonyl. |
| 16. | 16 The process for preparing a pregnane derivative substituted in the 16position which comprises: (1) reacting prednisolone with triethylorthoacetate to produce (I) prednisolone17, 21ethylorthoacetate; (2) reacting (I) with sodium acetate buffer in methanol to produce (II) prednisolone 17acetate; (3) reacting (II) with acetic anhydride in pyridine to produce (III) prednisolone17, 1diacetate; (4) reacting (III) with potassium acetate in dimethyl formamide to produce (IV) 16,17dehydroprednisolone21acetate; and (5) reacting (IV) with potassium cyanide in dimethylsulfoxide to produce (V) 2lacetoxy16acyanollβhydroxy3,20dioxol, 4 pregnadiene. |
| 17. | 17 The process of Claim 15 which additionally comprises re¬ acting (V) with hydrogen chloride to produce (VI) 16acyanollβ , 21dihydroxy3,20dioxol,4pregnadiene. |
| 18. | 18 The process of Claim 16 which additionally comprises re¬ acting (VI) with 2,3dihydropyran and pyridinium tosylate to produce (VII) 21tetrahydropyranyl16acyanollβhydroxy3,20 dioxo1,4pregnadiene. |
| 19. | 19 The process of Claim 17 which additionally comprises reacting (VII) with sodium hydroxide to produce (VIII) 21tetra hydropyranyl16acarboxyllβhydroxy3, 2ϋdioxol,4pregnadiene. |
| 20. | 20 The process of Claim 18 which additionally comprises reacting (VIII) with sulfuric acid and methanol to produce (IX) 16αmethoxycarbonylllβ , 21dihydroxy3,20dioxol,4pregnadiene. |
| 21. | 21 The process of Claim 19 which additionally comprises reacting (IX) with acetic anhydride to produce (X) 21acetoxy 16αmethoxycarbonylllghydroxy3,20dioxol,4pregnadiene. |
| 22. | 22 The process of Claim 15 which additionally comprises reacting (V) with hydrogen chloride to produce (XI) 21acetoxy 16 amino carbonyl3, 20dioxollBhydroxy1,4pregnadiene. |
| 23. | 23 The process of Claim 18 which additionally comprises reacting (VIII) with hydrogen bromide to produce (XII) l6acarboxy 17abromollβ , 21dihydroxy3,20dιoxol,4pregnadiene. |
| 24. | 24 The process of Claim 22 which additionally comprises reacting (XII) with sodium hydroxide to produce (XIII) 16a carboxyllβ , 17a, 21trihydroxy3,20dioxol,4pregnadiene. 2J. The process of Claim 23 which additionally comprises reacting (XIII) with diaxomethane to produce (XIV) 16amethoxy σarbonylllβ , 17a, 21tπhydroxy3 ,20dioxol,4pregnadiene. 26. The process for preparing a pregnane derivative sub¬ stituted in the 21position which comprises: (1) reacting 21carboxyllβ , 17a, 21trihydroxy3one 1,4pregnadiene with N,N'dicyclohexylcarbodiamide, 1hydroxy benzotriazole, and RNH2 wherein R is methyl, ethyl, npropyl, or benzyl to produce (I) 21carboxamidellβ , 17a, 21trihydroxy3 onel,4pregnadiene wherein the carboxamide group has the formula CONHR with R having the meaning given above; and (2) reacting (I) with manganese dioxide in tetrahydro furan to produce (II) 21carboxamidellβ, 17adihydroxy3 , 20 dιone1,4pregnadiene. |
Although the beneficial effects of natural and semi- synthetic corticosteroids in the treatment of inflammatory and allergic conditions have been appreciated for over 35 years, clinical use of glucocorticoids as anti-inflammatory agents is limited due to their adverse systemic effects. Potent synthetic glucocorticoids with minimum salt-retaining activity have been produced by structural modifications such as introduction of a double bond, a methyl group or halogen atom on the steroid nucleus. However, little success has been achieved in separating the anti-inflammatory effects of steroids from their adverse systemic effects. These short¬ comings are largely inherent in the nature of steroids them¬ selves; not only do they possess multiple biological activi¬ ties, but the structural requirements for the various activi¬ ties seem to be overlapping and inseparable.
Structural modifications of the corticosteroids have been suggested to improve properties of these compounds by reducing their adverse systemic effects while retaining their anti-inflammatory properties. Another approach to minimize adverse systemic effects of steroids has involved the use of various dosage forms for local administration. Local corti- costeroid preparations have been applied topically for treat¬ ment of skin disorders, injected intra-articularly for certain joint diseases, and applied as drops for ophthalmic disorders and sprayed for asthmatic conditions.
Although systemic effects are known to be reduced when conventional steroids are applied topically, the use of ster¬ oids in large quantities for prolonged periods results in toxic systemic side effects. All clinically effective topical corticosteroids have the potential for suppressing pituitary- adrenal
SUBSTTTϋTε SHggf
function and the immune system, especially when large amounts are topically applied under occlusion or on an extensive area. Topical steroids are readily absorbed if the skin is damaged by disease or injury. Children are particularly prone to the systemic effects of local steroid application and suppression of pituitary-adrenal function including growth impairment has been reported. In addition, complications associated with local steroid treatment for psoriatic, rheumatologic, exzema- tous!, asthmatic and ophthalmic disorders have been observed.
One structural modification was suggested in U.S. Patent 3,944,577 to Laurent et al. wherein structural modifi¬ cations of the ketol side chain on the pregnane molecule were suggested, principally the combination of a 20- carbonyl group and a 21- carboxylic acid or ester group. This provided some alleviation of the adverse systemic effects, but the compounds did not provide sufficient improvement to be widely accepted as substitutes for cortisol or prednisolone.
In my copending patent application Serial No. 502,449 filed June 9, 1983, there are described certain prednisolone- 21- carboxylic acid derivatives which were equivalent to pred¬ nisolone in anti-inflammatory activity and exhibited remark¬ able improvements with respect to the adverse side effects, e.g., reduced suppression of adrenal and thymus weights, better production of liver glycogen, better skin collagen synthesis, and reduced suppression of granuloma formation. Nevertheless, still greater improvement was required to pro¬ vide greater acceptance for these new compounds.
It is an object of this invention to provide novel pregnane derivatives having a carboxy ester or amide group at one or more of the strategic 6α-, 6g-, 9α-, 16α-, 16β-, or 20- positions as safer anti-inflammatory steroids. It is another object of this invention to provide novel processes for the synthesis of such steroids. Other objects will appear from the more detailed description which follows.
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BRISF DESCRIPTION OF THE INVENTION This invention relates to carboxy pregnane derivatives of the formula:
wherein one X is COOR, CH 2 COOR, CH(COOR) 2 , CONHR, CH j CONHR, or CN;
remaining X's are H, F, CH 3 , OH, COOR, CH 2 COOR, CH{COOR) 2 , CONHR, CHjCONHR, or CN;
Y is
R_
I
C=0
R is H, alkyl of 1-5 carbon atoms, or benzyl; ~ l is CH 2 OR 3 , COOR, or CONHR; R 2 is H, 0R 3 , or Br;
R 3 is H, CORt, , or tetrahydropyranyl; i, is alkyl of 1-5 carbon atoms or benzyl; represents a single or double bond;
Λ represents α-position, 8-positιon, or a mixture of both - and ~ -positions; and represents α-position.
In certain preferred embodiments of this invention, the derivatives can be represented by the formula:
R.
(ID c=o
wherein Ri and R 2 have the same meanings as in Formula (I) above and x is COOR, CH 2 COOR, CH(COOR) 2 , CONHR, CH 2 CONHR, or CN.
This invention also relates to processes for preparing cortisol and prednisolone derivatives in which various carboxy substituents (x in Formula (II)) are present in the 6a- , 6β-, 9α-, 16α-, 16β , and 20- positions on the molecule.
DETAILED DESCRIPTION OF THE INVENTION
The compounds of this invention are described by five formulas given below
(VI)
X wherein all symbols have the same meaning as given for Formula (I) above.
These compounds all provide improved properties for use as an anti-inflammatory drug. The improvement resides principally in greater reductions in the adverse side effects than have been observed in previously known compounds. All of the compounds are carboxy pregnane derivatives, and more specifically, the derivatives of cortisol or of prednisolone.
Among the preferred compounds for anti-inflammatory uses with minimal side effects are those of the formula:
R 6 is CH 2 0H, COOH, C0NH 2 , CO HCH3 , COOCH 3 , or CH 2 COOCH 3 ;
R 7 is H or COR 13 ;
R 8 is H or COCH 3 ;
R 9 is Br, OH, or 0C0CH 3 ;
Rio is Br, OH, or OCORi 3 ;
Rll is CH(COOCH 3 .2, CH(C00H) 2 , C0NH 2 , CHCOOCH 3 , CHCOOH,
COOH, or CN;
R12 is H or CH 2 COOR 13 7 and
R13 is alkyl of 1-5 carbon atoms or tetrahydropyranyl.
The most desirable of all of the compounds of this invention for anti-inflammatory uses are those of the formula:
COOR j i
wherein Rji, is alkyl of 1-5 carbon atoms, and R ϊ 5 is OH or OCORji f .
Among the specific compounds which are included in this invention are the following illustrative compounds. It is to be understood that the invention is not limited to these named compounds, but that these merely represent various substituents which may be combined in many ways. The numbering of the com- " pounds follows the structural formula given below:
PREDNISOLONE, DERIVATIVES
llβ, 17α, 20α- tπhydroxy -6 -fluoro-16o-aminocarbonyl-3-one-
1, 4-pregnadien-21-oic acid;
Methyl llβ, 17α, 20α-trihydroxy -6α-fluoro-16α-aminocarbonyl-3- one-1, 4-pregnadien-21-oate;
Ethyl llβ, 20-dihydroxy-6β-fluoro-16α-aminocarbonyl-17α-methyl- carbonyloxy-3-one-l, 4-pregnadien-21-oate; n-Propyl llβ, 20-dihydroxy-6β-fluoro-16α-methylaminocarbonyl-17α- ethylcarbonyl-3-one-l, 4-pregnadien-21-oate; llβ, 17α, 20β-trihydroxy-6β-methyl-16β-cyano-3-one-l, 4-pregnadien-
21-oic acid;
llβ , 17α , 20β-trihydroxy-6β -ethyl-16 -cyano-3-on3-l, 4-preg- nadien-21-oic acid;
Methyl llβ , 17α , 20β -trihydroxy-6c. -methyl-16α-cyano-3-on3-l , 4-pregnadien-21-oate;
Ethyl llβ , 20-dihydroxy-6β -methyl-16β -cyano-17α -methylcarbonyloxy- 3-one-l, .4-pregnadien-21-oate; llβ , 17α-dihydroxy-9α-fluoro-16β -ethoxycarbonyl-20α-acetoxymethyl- 3-one-l , 4-pregnadien-21-oic acid ;
Methyl llβ , 17α-dihydroxy-9α -fluoro-16β -methoxycarbonyl-20β - acetoxymethyl-3-one-l , 4-pregnadien-21-oate;
Ethyl llβr hydroxy-9c.-fluoro-16β-methoxycarbonyl-17c_-methylcar- bonyloxy-20α-acetoxymethyl-3-one-l, 4-pregnadien-21-oate; llβ, 17α , 20α-trihydroxy-16α-methoxycarbonylmethyl-3-one-l, 4- pregnadien-20-carboxamide;
N-methyl llβ, 17<_, 20c.-trihydroxy-16cι-methoxycarbonylmethyl-3- one-1, 4-pregnadien-20-carboxamide; llβ, 17<_, 20β-trihydroxy-16β-(dicarboxy) methyl-3-one-l, 4- pregnadien-2O-carboxajiiide;
N-benzyl llβ, 17α, 20α-trihydroxy-16α-cyano-3-one-l, 4-pregnadien -20-carboxamide;
N-propyl llβ, 20β-dihydroxy-17 α -bromo-16β-di(methoxycarbonyl) methyl-3-one-l, 4-pregnadien-20-carboxamide;
Methyl llβ, 17α, 20β-trihydroxy-16α-aminocarbonyl-3-one-l, 4- pregnadien-20-acetate; llβ, 17o, 21-trihydroxy-6ct-fluoro-16β-aminocarbonyl-3, 20-dione- 1, 4-pregnadiene; llβ, 17α, 21-trihydroxy-16α-methoxycarbonylmethyl-3, 20-dione-l, 4-pregnadiene; llβ, 17α-dihydroxy-16β-aminocarbonyl-21-tetrahydropyranyloxy-3, 20-dione-l, 4-pregnadiene; llβ, 17α-dihydroxγ-16β-di(methoxycarbonyl) methyl-21-acetoxy-3, 20-dione-l, 4-pregnadiene; llβ, 21-dihydroxy-16α-cyano-17α-bromo-3, 20-dione-l, 4-pregnadien llβ, 17α, 21-trihydroxy-16 α -carboxy-3, 20-dione-l, 4-pregnadiene;
llβ, 17α-dihydroxy-9o-fluoro-16α-methoxycarbonyl-3, 20-dione-l, 4-pregnadien-20-carboxamide; llβ-hydroxy-9α-fluoro-16α-ethoxycarbonyl-17o-methylcarbon yloxy- 3, 20-dione-l, 4, pregnadien-20-carboxamide; N-methyl llβ, 17o-dihydroxy-6β-methyl-16β-methoxycarbonyl-3, 20-dione-l, 4-pregnadien-20-carboxamide; llβ, 17α-dihydroxy-9α-fluoro-16α-methoxycarbonyl-20-methoxymet hyl -3, 20-dione-l, 4-pregnadiene; llβ-hydroxy-9α-fluoro-16α-ethoxycarbonyl-17α-methylcarbo nyloxy- 20-ethoxymethyl-3, 20-dione-l, 4-pregnadiene; llβ-hydroxy-9α-fluoro-16α-butoxycarbonyl-17α-i-propylcar bonyloxy- 20-pentoxymethyl-3 , 20-dione-l, 4-pregnadiene; llβ , 17α-dihydroxy-9α-fluoro-16α-pentoxyσarbonyl-20-n-propoxy methyl -3-20-dione-l, 4-pregnadiene;
17α, 20, 20, 21-bis-(methylenedioxy) -6α-methoxycarbonyl-3-oxo-l, 4-pregnadien-llβ-ol;
17 , 20, 20, 21-bis(methylenedioxy) -6α-methoxy-carbonylmethyl-3- oxo-1, 4-pregnadien-llβ-ol;
17α, 20, 20, 21-bis(methylenedioxy) -16β-cyano-3-oxo-l, 4- pregnadien-llβ-ol;
Methyl llβ-hydroxy-17-20α-isopropylidendioxy-6α-methoxycarbonyl- methyl-3-oxo-l, 4-pregnadien-21-oate;
Methyl llβ-hydroxy-17, 20α-cyclocarbonyloxy-9α-fluoro-6β-ethoxy- carbonylmethyl-3-oxo-l, 4-pregnadien-20-oate; llβ-hydroxy-17, 20β-cyclocarbonyloxy-6β-methylaminocarbonyl-21- ethoxymethyl-3-oxo-l, 4-pregnadiene; llβ-hydroxy-17, 21-cyclocarbonyloxy-6β-methoxycarbonylmethyl-3 -oxo-1, 4-pregnadiene;
CORTISOL DERIVATIVES
llβ, 17α, 20α-trihydroxy-6β-fluoro-16β-methoxycarbonyl-3-one-4- pregnen-21-oic acid; llβ, 17α, 20β-trihγdroxy-6α-methyl-16α-aminocarbonyl-3-one-4- pregnen-21-oic acid;
9α , llβ , 17ix-trihydroxy-6β-benzoxycarbonyl-3-one-4-pregnen-
21-carboxamide ;
N-Ethyl llβ , 17 , 20α-trihydroxy-16a-methoxycarbonylmethyl-3- one-4 -p egnen- 20 -carboxamide ;
Butyl llβ , 17α , 20β -trihydroxy-16β - (dicarboxy) methyl-3-one-
4-pregnen-21-oate;
N-Benzyl llβ , 17 α , 20β -trihydroxy-6 -methoxycarbonylmethyl-3- one-4-pregnen-20-carboxamide;
Methyl llβ -hydroxy-6β -f luoro-17 α -acetoxy-3-one-4-pregnen-21- acetate;
9α , llβ , 21-trihydrδxy-17α -acetoxy-20β -acetoxymethyl-3-one-4- pregnene;
Methyl llβ , 16β , 17α -trihydroxy-6α -benzaminocarbonylmethyl-3- one-4-pregnen-21-oate; llβ , 17 α , 21-trihydroxy-6β -fluoro-16c. -methoxycarbonyl-3 , 20- dione-4-pregnene; llβ, 17 , 21-trihydroxy-9-methyl-16β-di(butoxycarbonyl) methyl
-3, 20-dione-4-pregnene; llβ , 17α-dihydroxy-16 -pentaminocarbonylmethyl-21-tetrahydro- pyranyloxy-3, 20-dione-4-pregnene;
9, llβ,.21-trihydroxy-16α-cyano-17α-bromo-3, 20-dione-4-pregnene; llβ, 17α, 21-trihydroxy-16β-carboxy-3, 20-dione-4-pregnene;
17α, 20, 20, 21-bis(methylenedioxy) -6α-methoxycarbonyl-3-oxo-
4-pregnen-llβ-ol;
17_ , 20, 20, 21-bis(methylenedioxy)-6β-methoxycarbonylmethyl-3
-oxo-4-pregnen-llβ-ol;
17α, 20, 20, 21-bis(metbylenedioxy)-6β-fluoro-16β-di(methoxy- carbonyl)methyl-3-oxo-4-pregnen-llβ-ol;
Ethyl llβ-hydroxy-17α-20o-isopropylidendioxy-6α-propoxycarbonyl - methyl-3-oxo-4-pregnen-21-oate;
Methyl llβ-hydroxy-17α, 20β-cyclocarbonyloxy-9α-fluoro-6β- methoxycarbonylmethyl-3-oxo-4-pregnen-20-oate;
9, llβ-dihydroxy-17α , 20β-cyclocarbonyloxγ-16α-cyano-21- benzoxymethyl-3-oxo-4-pregnene; and llβ-hydroxy-17α , 21-cyclocarbonyloxy-6α-butylaminocarbonyl- methyl-3-oxo-4-pregnene.
The process for preparing pregnane derivatives of this invention substituted in the 6-position proceeds as follows:
(1) Cortisol or prednisolone is reacted with for¬ maldehyde and hydrogen chloride in the presence of chloroform to produce two dioxolane cycles in the 17-, 20-, and 21- positions; e.g., (I) 17α , 20, 20, 21-bis(methylenedioxy) - llβ-hydroxy-pregn-4-ene-3-one;
(2) (I) is reacted with ethylene glycol and pyridine hydrochloride in solution in benzene to produce another di¬ oxolane cycle at the 3-position, e.g., (II) 3, 3-ethylenedioxy -17o, 20, 20, 21-bis(methylenedioxy) -llβ-hydroxy-pregn-5-ene -llβ-ol;
(3) (II) is reacted with M-chloroperbenzoic acid in chloroform to epoxidize the 5-double bond, producing, e. g., (Ill) 5, 6 α -epoxy-3, 3-ethylenedioxy-17<_, 20, 20, 21-bis (methylenedioxy)-5 α -pregnan-llβ-ol;
(4) (III) is reacted with an alkyl agnesium bromide in tetrahydrofuran to break the epoxy ring and add the alkyl group at the 6-position, e.g., to produce (IV) 3, 3-ethylenedioxy -17α, . 20, 20, 21-bis(methylenedioxy) -6β (prop-2-enyl) -5α-pregnane -5, llβ-diol;
(5) (IV) is reacted with sodium iodate and potassium permanganate in t-butanol to oxidize the alky group to a carboxylic acid to produce, e.g., (V) 3, 3-ethylenedioxy-17α , 20, 20, 21-bis(methylenedioxy)-6β-carboxymethyl-5α-pregnane-5, llβ-diol;
(6) (V) is reacted with diazomethane in methanol to esterify the acid to produce e.g., (VI) 3, 3-ethylenedioxy-17ct,
20, 20, 21-bis(methylenedioxy)-6β-methoxyσarbonylmethyl-5α-pregna ne- 5, llβ-diol;
(7) (VI) is reacted with strong sulfuric acid in acetone to reduce the dioxolane structure at the 3-position to a carbonyl to produce, e.g. (VII) 17α, 20, 20, 21-bis(methylenedioxy)-6β- methoxycarbonylmethyl-5, llβ-dihydroxy-5o-pregnan-3-one;
(8) (VII) is then treated with an activated magnesium silicate in benzene to remove the 5-hydroxyl to produce e..g.,
(VIII) llβ-hydroxy-6β-methoxycarbonylmethyl-17α, 20, 20, 21-bis (methylenedioxy) -pregn-4-en-3-one;
(9) (VIII) is reacted with formic acid to break the two dioxolane groups at the 17-, 20-, and 21- positions so as to return to the structure originally present at these positions in the cortisol or prednisolone starting material to produce (e.g.)
(IX) llβ, 17α-21-trihydroxy-6β-methoxycarbonylmethyl-4-pregnen-3, 20-dione; and
(10) if it is desired to produce the same structure as (IX) in the 1, 4-pregnadiene molecule,, (VIII) is reacted first with 2, 3-dichloro-5, 6-dicyanoquinone to produce the double bond in the 1-position and then reacted with formic acid to remove the two cyclic dioxolane structures to produce, e.g., (X) llβ, 17α, 21-trihydroxy-6β-methoxycarbonylmethyl-l, 4-pregnadien -3, 20-dione.
The process for preparing pregnane derivatives of this invention substituted in the 16-position proceeds as follows:
(1) Cortisol or prednisolone is reacted with triethylortho- acetate and pyridine tosylic acid to make a cyclic structure joining the 17- and 21-positions, e.g., to produce (I) llβ-hydroxy-17, 21 cyclocarbonyloxy-1, 4-pregnadien-3, 20-dione;
(2) (I) is reacted with-a sodium acetate buffer in methanol to break the cyclic structure and substitute an acetate group at the 17-position to produce, e.g., (II) llβ, 21-dihydroxy-17α-acetoxy-l, 4-pregndien-3, 20-dione;
(3) (II) is reacted with acetic anhydride in pyridine to place an acetate group on the 21-position to produce, e.g., (Ill) llβ-hydroxy-17α, 20-diacetoxy-l, 4-pregnadien-3, 20-dione;
(4) (III) is reacted with potassium acetate in dimethyl- formamide to remove the acetoxy group from the 17-ρosition to produce e.g., (IV) llβ-hydroxy-20-acetoxy-l, 4-pregnadien-3, 20-dione;
(5) (IV) is saponified with sodium hydroxide to produce the corresponding structure with 20-hydroxy (V) and subsequently reacted with 2, 3-dihydropyran and anhydrous pyridinium tosylate to produce e.g., (VI) llβ-hydroxy-20-tetrahydropyranyl-l, 4- pregnadien-3, 20-dione;
(6) VI is reacted with sodium methylate and dimethyl- malonate to substitute in the 16-position to produce e.g., (VII) 21-tetrahydropyranyl-16-yl(dimethylmalonyl) -llβ-hydroxy-3, 20- dioxo-1, 4-pregnadiene, which is treated with hydrogen chloride to transpose it to the corresponding 21-hydroxy compound (VIII) ;
(7) (VII) is treated with sodium hydroxide, and subse- - quently with an acid to produce the corresponding compound with malonic acid in the 16-position, e.g., (IX) 21-tetrahydropyranyl- 16-dicarboxyrαethyl-llβ-hydroxy-3, 20-dioxo-l, 4-pregnadiene;
(8) (IX) is treated with hydrogen chloride to produce (X) llβ, 21-dihydroxy-16-dicarboxymethyl-3, 20-dioxo-l, 4-pregnadiene;
(9) (IV) is reacted with potassium cyanide in dimethyl- sulfoxide to produce the corresponding 16-cyano compound, e.g., (XI) 21-acetoxy-16α-cyano-llβ-hydroxy-3, 20-dioxo-l, 4-pregnadiene, which is acidified with hydrogen chloride to produce (XII) 16α- cyano-llβ, 21-dihydroxy-3, 20-dioxo-l, 4-pregnadiene, which is treated with 2, 3-dihydroρyran and anhydrous pyridinium tosylate to produce (XIII) 21-tetrahydropyranyl-16α-cyanσ-llβ-hydroxy-3, 20-dioxo-l, 4-pregnadiene;
(10) (XIII) is reacted with sodium hydroxide to produce (XIV) 21-tetrahydropyranyl-16α-carboxy-llβ-hydroxy-3, 20-dioxo-l, 4-pregnadiene, which is reacted with sulfuric acid and methanol to produce, e.g., (XV) llβ, 21-dihydroxy-16-methoxycarbonyl-3, 20- dioxo-1, 4-pregnadiene;
(11) (XV) is reacted with acetic anhydride to produce XVI 21-acetoxy-16α-methoxycarbonyl-llβ-hydroxy-3, 20-dioxo-l, 4-pregnadiene;
(12) XI is reacted with hydrogen chloride to produce e.g. (XVII) 21-acetoxy-16α-aminocarbonyl-llβ-hydroxy-3, 20-dioxo- 1, 4-pregnadiene;
(13) (XIV) is reacted with hydrogen bromide or copper bromide to produce (XVIII) llβ, 21-dihydroxy-16a-carboxy-17α- bromo-3, 20-dioxo-l, 4-pregnadiene; and
(14) (XVIII) is reacted with sodium hydroxide to produce e.g., (XIX) llβ, 17ct, 21-trihydroxy-16 -carboxy-3, 20-dioxo-l, 4-pregnadiene, which is reacted with diazomethane to produce e.g., (XX) llβ, 17α, 21-trihydroxy-16α-methoxy-carbonyl-3 , 20- dioxo-1, 4-pregnadiene.
The process for preparing pregna_ne derivatives of this invention substituted in the 20-position proceeds as follows:
(1) Cortisol or prednisolone with 20-hydroxy and 21- carboxy groups are known, e.g., (I) llβ, 17 , 20-trihydroxy-20- carboxy-3-oxo-l, 4-pregnadiene; and (I) is reacted with N, N'- diσyclohexylcarbodiimide, hydroxybenzotriazole, and a primary amine to produce the corresponding llβ, 17α, 20-trihydroxy-20- aminocarbonyl-3-oxo-l, 4-pregnadiene; when the amine is methyl- amine the product is (II) the 20-N-methylaminocarbonyl of the named compound; when the amine is ethylamine the product is (III) the 20-N-ethylaminocarbonyl of the named compound; when the amine is N-propylamine the product is (IV) the 20-N-propylaminocarbonyl of the named compound; and when the amine is benzylamine the product is (V) the 20-N-benzylaminocarbonyl of the above named compound.
(2) (II), (III), (IV), or (V) is reacted with manganese dioxide in tetrahydrofuran to produce the corresponding (VI) llβ, 17α-dihydroxy-20-aminocarbonyl-3, 20-dioxo-l, 4-pregnadiene;
(3) The original cortisol or prednisolone is reacted with acetic anhydride in pyridine to produce e.g., the known compound (VII) llβ, 17α-dihydroxy-21-acetoxy-3, 20-dioxo-l, 4-pregnadiene;
(4) (VII) is reacted with sodium borohydride in methanol to produce e.g., (VIII) llβ, 17a, 20-trihydroxy-21-acetoxy-3-oxo-l, 4-pregnadiene, which is saponified to produce (IX) llβ, 17α, 20, 21-tetrahydroxy-3-oxo-l, 4-pregnadiene; and
(5) (VIII) is reacted with perchloric acid in acetone to produce e.g., (IX) 21-acetoxy-ll-hydroxy-ly, 20-isopropylidendioxy -3-oxo-l, 4-pregnadiene which is saponified to (X) llβ, 21-dioxy- 17, 20α-isopropylidendioxy-3-oxo-l, 4-pregnadiene.
In the following examples there are illustrations of the above procedures. Parts and percentages are by weight unless otherwise described. Temperatures are in degrees Centigrade unless otherwise specified. The specific identification of the α- or β-epimer is not intended to eliminate the other epimer from the illustration. In general the a-configuration occurs at the 9- and 17-positions, while both a- and β- are possibilities at the 6- and 16-positions. Example 1
5g. of 17a, 20, 20, 21-bis(methylenedioxy) -pregn-4-ene- llβ-ol, prepared by reacting cortisol with formaldehyde and hydrogen chloride in chloroform, is dissolved in 200 ml of ben¬ zene; ethyleneglycol (25 ml) and pyridine hydrochloride (300 mg) were added to the solution, and the mixture was refluxed with continuous removal of H 2 0 for 8 hours. The reaction mixture was diluted with ethyl acetate, washed with 5% NaHC0 3 and H 2 0, and dried over anhydrous Na j SO^ . After evaporation of the solvent, the product was submitted to column chromatography on silica gel. Elution with benzene-ether (20:1) as the eluent gave 3.1g of 3, 3-ethylenedioxy-17a, 20, 20, 21-bis(methylenedioxy) -pregn- 5-ene-llβ-ol. NMR(CDCl 3 ) <5 : 1.06(3H,s,18-CH 3 ) , 1.26 (3H,s,19-CH 3 ) ,
3.90(4H,s,ethylenedioxγ) , 3.95(2H,s,21-CH 2 ) , 4.40(lH,m,ll -H) , 4.96, 4.98, 5.15(5H,m,C 6 -H and bis(methylenedioxy) ) .
Example 2
A solution of the product of Example 1 (1.06g) and m-chloroperbenzoic acid (540 mg) in CHCl 3 (8 ml) was stirred at room temperature for 3 hours. The reaction mixture was diluted with ethyl acetate, washed with 5% NaHS0 3 , 5%. NaHC0 3 , and H 2 0, and dried over anhydrous Na 2 S0i f . After evaporation of the solvent, the crude product obtained was submitted to column chromatography on silica gel using hexane/ethyl acetate (2:1) as the eluent. The first eluate, which is the less polar product, gave 410 mg of 5,6β- epoxy-3,3-ethylenedioxy-17a, 20, 20, 21-bis(methylenedioxy) - 5β- pregnan-llβ-ol (Product 2B) . NMR (CDC1 3 )5: 1.06(3H,s,18-CH 3 ) , 1.28(3H,s,19-CH 3 ) , 3.07 (lH,m,6a-H) , 3.90(4H,s,ethylenedioxy) , 3 * .96(2H,s,21-CH 2 ) , 4.25(lH,m,llα-H) , 4.98, 5.15(1H and 3H, each s,bis(methylenedioxy) ) . The second eluate, which is the more polar product, gave 730 mg of 5, 6a-epoxy-3, 3-ethylenedioxy-17 , 20, 20, 21-bis(methylenedioxy)-5a-pregnan-llβ-ol (Product 2A) . NMR (CDCl 3 )δ: 1.02(3H,s,I8-CH 3 ) , 1.35(3H,s,19-CH 3 ) , 2.74 (lH,rα,6 -H) , 3.93(6H,s,3,3 -ethylenedioxy) , 4.25(lH,m,ll -H) , 4.96, 5.13 (1H and 3H, each s, 17 , 20, 20, 21-bis(methylenedioxy) ) . Example 3
A solution of allylmagnesium bromide in tetrahydrofuran(THF) was prepared from Mg (0.16 mol) and allyl bromide (0.08 mol) by the method of Grumitt et al and stored at 0° under nitrogen. A solution of Product 2A of Example 2 (113 mg) and the Grignard re¬ agent (5 ml) in anhydrous THF (5 ml) was refluxed for 30 min. After addition of cold saturated NH^Cl to decompose the excess reagent, the resulting solution was extracted with ethyl acetate. The organic layer was washed with 5% NaHC0 3 and H 2 0, and dried over anhydrous Na 2 S0ι t . After evaporation of the solvent, the crude product was submitted to column chromatography on silica gel using benzene-acetone (50:1) as the eluent. Recrystalliza- tion of the eluate from acetone-MeOH gave (53 mg) of 3, 3- ethylenedioxy-17α, 20, 20, 21-bis(methylenedioxy) -6β (prop-2-enyl) - 5a-pregnane-5, llβ-diol as colorless needles, .p. 193-199°. IR -m x° lc ~ 1: 3410 (OH) . NMR (CDCl 3 )δ: 1.07 (3H,s,18-CH 3 ) , 1.20 (3H,s,19-CH 3 ) , 3.93(6H,s,C 21 -CH 2 and 3,3-ethylenedioxy) , 4.26
(2H,m,ll -H and 5 -OH), 4.85, 4.97(2H,m,-CH=CH 2 ) , 4.97, 5.15 (3H and IH, each s, 17 , 20, 20, 21-bis(methylenedioxy) ) . Example 4
To 100 mg of a solution of the product of Example 3 in t-butanol there was added 0.8% KMnOtj (4 ml) and NalO (100 mg) in t-butanol (9 ml) , and the mixture was stirred at room tem¬ perature for 30 min. The reaction mixture was acidified with 5% HC1 and was then extracted with ethyl acetate. The organic layer was washed with 5% aHCθ 3 , and H 2 0, and dried over an¬ hydrous After evaporation of the solvent the product was purified by column, chromatography using benzene-acetone (50:1) as the eluent to give 70 mg of 3,3-ethylenedioxy-17a, 20, 20, 21-bis(methylenedioxy)-6β-carboxymethyl-5a-pregnane-5, llβ-diol. 70 mg) . IR]^^cπf 1 : 3450 (OH), 1700(COOH). NMR (CDC1 3 ) : 1.06 (3H,s,18-CH 3 ) , 1.17(3H,s,19-CH 3 ) , 3.93 (6H,s,21-CH 2 and ethylenedioxy), 4.23(lH,m,ll -H) , 4.99, 5.16(3H and IH, each s, bismethylenedioxy) . Example 5
An etheral solution of CH 2 N 2 (1 ml) was added to a solution of the product of Example 4 (70 mg) in methanol. Evap¬ oration of the solution under the reduced pressure gave 70 mg of 3,3-ethylenedioxy-17, 20, 20, 21-bis(methylenedioxy)-6β-methoxy- carbonylrαethyl-5a-pregnane-5, llβ-diol (70 mg) . IRl f ° cm " : 3370 (OH), 1730 (C00CH 3 ). NMR (CDCl 3 )δ: 1.06 (3H,s,I8-CH 3 ) , 1.20 (3H,s,19-CH 3 ) , 3.63 (3H,s,C00CH 3 ) , 3.93(6H,s,21-CH 2 and ethylene¬ dioxy), 4.25(lH,m,ll -H) , 4.99, 5\15(3H and IH, each s,bismethylene¬ dioxy) . Example 6
2N H 2 SO tt (0.05 ml) was added to a solution of the product of Example 5 (50 mg) in acetone (1.5 ml), and the mixture was stirred at room temperature for 1 hour. After addition of 5% NaHC0 3 to neutralize, the resulting solution was extracted with ethyl acetate. The organic layer was washed with H 2 0 and dried over anhydrous Na 2 S0i t . Evaporation of the solvent gave 30 mg of 17a, 20, 20, 21-bis(methylenedioxy)-6β-methoxycarbonyl- methyl-5,llβ-dihydroxy-5a-pregnan-3-one. IRl^^^m -1 : 3400(OH) ,
1700 (=C0, COOCH ). NMR (CDC1 ) : 1.10(3H,S,18-CH ), 1,43 (3H,s,18-CH ), 3.65(3H,s,COOCH ) , 3.95(2H,m,21-CH ), 4.28 (lH,m,ll -H) , 5.00, 5.16(3H and IH, each s,bismethylenedioxy) . Example 7
A solution of the product of Example 6 (50 mg) and 300 mg of activated magnesium silicate in benzene (10 ml) was stirred at 100°C for 5 hours. After removal of the silicate by filtration, the filtrate was concentrated under the reduced pressure. The product obtained was submitted to column chroma¬ tography on silica gel using benzene-acetone (20:1) as the eluent. Recrystallization of the eluate from MeOH gave 20 mg of llβ- hydroxy-6β-metl.oxycarbonylmethyl-17 ,20,20,21-bis(methylenedioxy) - pregn-4-en-3-one as colorless prisms, m.p. 217-200°C. IR]max - cm :
3420 (OH) , 1720 (COOCH 3 ), 1650 ( α ,β-unsaturated ketone) . NMR
(CDCl 3 )θ: 1.18(3H,s,18-CH 3 ) , 1.51(3H,s,19-CH 3 ) , 3.68 (3H,s,COOCH 3 ) ,
4.02(2H,m,21-CH 2 ) , 4.25(lH,m,ll -H) , 5.06, 5.25(3H and IH, each s,bis(methylenedioxy) ) , 5.78 (lHjS f Ci f -H) .
Example 8
A suspended solution of the product of Example 7 (0.75 g) in 60% aqueous formic acid (21 ml) under nitrogen was heated in a water bath for 5 minutes. The reaction mixture was cooled, diluted with ice water (200 ml) and extracted with ethylacetate three times. The combined ethylacetate extract was washed with 2% NaHC0 3 and H 2 0, and dried over Na 2 S0t f and evaporated to dryness under vacuum. Recrystallization from ethylacetate gave 0.33 g of llβ , 17 ,21-trihydroxy-6β-methoxycarbonylmethyl-4-pregnane-3, 20- dione. NMR (CDCl 3 )δ: 1.18(3H, s,18-CH s ) , 1.45(3H,s,18-CH 3 ) , 3.68(3H,s,COOCH 3 ) 4.15(2H,d,21-CH S ) , .25(lH,m,ll-H) . Example 9
To a solution of the product of Example 7 (4 g) in 120 ml of dry benzene, 3.5 g of 2,3-dichloro-5,6-dicyanoquinone was added and refluxed for 24 hours. The reaction mixture was cooled and filtered. The filtrate was washed with 2% NaHC0 and water. The organic phase was dried with a 2 S0i t and evaporated to dryness under vacuum. The crude product was subjected to silica gel column
chromatography using benzene: ethylacetate (l:l,v/v) as eluting solvent. After combining the fractions corresponding to product, the solvent was evaporated to dryness. Recrystallization from ether-hexane yielded 2.4 g of llβ-hydroxy-6β-methoxycarbonylmethyl- 17a,20,20,21-bis(methylenedioxy) -pregn-l,4-diene-3-one. NMR (CHcl 3 )δ : 1.18(3H,s,18-CH 3 ) , 1.48 (3H,s,19-CH 3 ) 3.68(3H,s,COOCH 3 ) , 4.15(2H,d, 20-CH 2 ), 4.25(lH,m,ll-H) 5.06 and 5.25 (3H and IH, each s, bis (methylenedioxy) Example 10
A suspended solution of the product of Example 9 (1.5g) in 3.0 ml of 40% HF was kept in an ice box for 90 minutes. The reaction mixture was neutralized by adding 4% NaHC0 3 and then extracted with ethylacetate. The organic phase was washed with H 2 0, dried with Na 2 S0t f and evaporated to dryness under vacuum. Recrystallization from ethylacetate yielded 5.8g of llβ, 17a, 21-trihydroxy-6β- methoxycarbonylmethyl-l,4-pregnadien-3,20-dione. NMR (CHC1 3 ) δ : 1.18(3H,s,18-CH 3 ) , 1.45(3H,s,19-CH 3 ) , 3.68 (3H,s,C00CH 3 ) , 4.15(2H,d, 20-CH 3 ), 4.25(lH,m,ll-H) , 5.96(IH,t,4-H) 6.20(lH,d of d,2-H) 7.2 (lH,d of d, 1-H) . Example 11
To a solution of prednisolone (50 g) in 1 L benzene, there was added triethylorthoacetate (50 ml) and pyridinium tosylic acid (1.25g) . After distillation for 1.5 hours, the reaction mixture was kept in refrigeration to give colorless cubic crystal (about 100%) . Recrystallization twice from benzene gave llβ-hydroxy-17, 21-cyclocarbonyloxy-l,4-pregnadien-3,20-dione (as a prism) mp = 188-189°C. Example 12
To a solution (59g) of the product of Example 11 in 700 ml methaol there was added 400 ml 0.IN acetate buffer (10ml 0.IN acetic acid and 90 ml 0.IN sodium acetate) . After refluxing for 1 hour, methanol was evaporated and the product was extracted with ethylacetate. Evaporation of ethylacetate solution and crystallization from acetone/hexane gave colorless hexagonal crystals (26.lg, 47.3%) of 118,21-dihydroxy-17a-acetoxy-l,4-pregnadiene-3,20-dione. Analytical sample was obtained from recrystallization from acetone. mp=223-224°C
1H-NMR (CDCI 3 ) δ 0.98. (S, 3H, 13-CH 3 ), 1.45(S, 3H, IO-CH 3 ), 2.04 (S, 3H, 17-Ac) , 3.05 (m, IH, 21-OH) , 4.3 (m, 2H, 20-CH 2 O-) , 4.52 (m, IH, 11-H) , 6.04(S, IH, 4-H) , 6.29(dd, IH, J=10 and 2Hz, 2-H) , 7.25 (d, IH, J=10Hz, 1-H) . Example 13
To a solution of (25g) in 70 ml pyridine the product of Example 12 acetic anhydride (10ml) was added. After 2 hours at room temperature, 0.3N HCl (500 ml) was added. Extraction with ethylacetate, washing with 0.1% NaHC0 3 and evaporation afforded yellowish oil, which was kept at 4°C to give a crystal. Recrystall¬ ization from acetone/hexane gave colorless hexagonal crystals o ' f 17a,21-diaceto__y-llβ-hydroxy-l,4-pregnadiene-3,20-dione. (22.4g, 81.4%) mp=99.5-10-.5°, 190-191°C. Example 14
To a solution of the product of Example 13 (21.4g) in 250 ml dimethylformamide, potassium acetate (25g) was added. After stirring at 105°C under nitrogen for 6 hours, the reaction mixture was cooled down to room temperature and poured into ice water (3L) . After fil¬ tration, crystallization from acetone afforded 21-acetoxy-llβ- hydroxy-1, 4, 16 pregnatriene-3, 20-dione (17g, 89.8%) . Recrystall¬ ization gave yellowish long cubic, m.p. = 203-205°C. NMR (CDC1 3 ) δ 1.25 (s,3H, I3-CH 3 ), 1.48 (s, 3H, IO-CH3) , 2.18 (s, 3H, 21-Ac) , 4.40 (m, IH, 11-H) , 4.94(m, 2H, 20-CH 2 O-) . 6.02 (S, IH, 4-H) , 6.28 (dd, IH, J=10 and 2Hz, 2-H) , 6.74 (m, IH, 16-H) , 7.30 (d, IH, J=10 Hz, 1-H) . Upon normal saponification there was obtained llβ, 21- dihydroxy-1,4,16-pregnatriene-3,20-dione. Recrystallization from acetone gave needles (VI, 50.5%) . m.p. = 213-215°C; NMR (CDCI 3 ) δ 1.25(s, 3H, I3-CH 3 ) , 1.49 (s, 3H, IO-CH 3 ), 4.3.5-4.55 (m, 3H, 20-CH 2 - and 11-H) , 6.02 (S, IH, 4-H) , 6.28 (dd, IH, J=10 and 2Hz, 2-H) , 6.73 (m, IH, 16-H) , 7.32 (d, IH, J=10 Hz, 1-H) .
To a solution of llβ,21-dihydroxy-l,4,16-pregnatriene-3,20- dione (200 mg, 0.58 mmole) in dichloromethane (40ml) were added 2, 3-dihydropyran (0.2 ml, 2.19 mmole) and anhydrous pyridinium tosylate (70 mg, 0.32 mmole) . After stirring for 4 hours at room temperature, the reaction mixture was washed with water (100ml) and evaporated to give a yellowish oil. This oil was placed on
silica gel column and eluted with benzene ethyl acetate (2:1) . Evaporating the solvent afforded pure white foam of 21- tetrahydropyranyl-llβ-hydroxy-1,4,16-ρregnatriene-3,20-dio ne (191 mg. 77.1%), 1H-NMR (CDCl 3 ) δ 1.25 (s, 3H, 13-CH 3 ), 1.49 (s, 3H, IO-CH 3 ), 4.63-4.38 (m, 3H, 20-CH 2 - and 11-H) , 4.68 (t, IH, J=5Hz, -0-CH-0-) , 3.5, 3.85, 1.1 (m. pyranyl H) , 6.01 (s, IH, 4-H) , 6.27 (dd, IH, J=10 and 2 Hz, 2-H) , 6.73 (m, IH, 16-H) , 7.31(d, IH, J=10 Hz, 1-H) . Example 15
To a solution of the last product of Example 13 (lg, 2.34 mmole) in methanol ^ (20 ml) was added sodium dimethylmalonate (20 mmole) in 12 hour intervals, three times (performed with sodium methylate and dimethylmalonate) . After stirring for 48 hours at room temperature, the reaction mixture was diluted with ethylacetate (500 ml) and washed with water (300 ml, 200 ml) . Organics were evaporated to give a yellowish oil, which was further purified by silica gel column using acetone/hexane (1:2) . Recrystallization from acetone/hexane gave white prisms of 21-tetrahydropyranyl-llβ- hydroxy-lla-dimalonylmethyl-l,4-pregnadiene-3,20-dione (450 mg, 34.4%), mp = 194-197.5°C; 1H-NMR (CDCI 3 ) 0.99(s, 3H, 13-CH 3 ), 1.44 (s, 3H, IO-CH 3 ), 3.31 (m, IH, -CH-(COOCH 3 ) 2 ) , 3.67, 3.70 (s, 3H, 3H, malonate) . 4.24 - 4.06(m, 2H,20-CH 2 θ) , 4.42(m, IH, 11-H) , 4.66(m, IH, -0-CH-0-) , 6.01(s, IH, 4-H) , 6.27(dd, IH, J=10 and 2 Hz, 2-H) , 7.23(d, IH, J-lOHz, 1-H) .
To a solution of the foregoing product (200 mg, 0.38 mmole) in methanol (10 ml) was added 0.5N HC1 (1 ml) . After standing for 8 hours at room temperature, the reaction mixture was diluted with ethylacetate (200 ml) and washed with water (100 ml) . Drying on anhydrous sodium sulfate, followed by evaporating ethylacetate afforded a white solid (180 mg) . Recrystallization from acetone/ hexane gave 164 mg of white needles of llβ,21-dihydroxy-16-dimal- onylmethyl-l,4-pregnadiene-3,20-dione (83.5%), mp 192-196°C, 'H-NMR (CDC1 3 ) δ 0.98(s, 3H, 13-CH 3 ), 1.45(s, 3H, IO-CH 3 ) , 3.34(m, IH, - CH-(COOCH 3 ) ) , 3.64, 3.70(s, 6H, malonylmethyl) , 4.15 (d, 2H, J=3 Hz, 20-CH 2 ), 4.44(m, IH, 11-H) , 6.01(s, IH, 4-H) , 6.28(dd, IH, J=10 and 2Hz, 20-CH 2 O) , 7.22(d, IH, J=10 Hz, 1-H) .
Exa ple 16
To a solution of the first named product of Example 13 (1.4g, 3.64 mmole) in dimethylsulfoxide (30 ml) and water (5 ml) were added potassium cyanide (260 mg, 4.0 mmole) and ammonium chloride (216 mg, 4.0 mmole) . After stirring for 1.5 hours at 85°C, the reaction mixture was diluted with dichloromethane (600 ml) and washed with water (200 ml), 0.1N HC1 (200 ml) , 0.1N NaHC0 3 (200 ml) and water (200 ml) . After drying on anhydrous sodium sulfate, the organic layer was evaporated to dryness. Recrystalli¬ zation from acetone gave 685 mg colorless needles of 21 acetoxy- 16a-cyano-llβ-hydroxy-l, 4-pregnadiene-3,20-dione (45.7%), m.p. 239-242°C, "H-NMR (CDCl 3 ) δ 0.94 (s, 3H, 13-CH 3 ) . 0.46 (s, 3H, 10- CH 3 ) , 2.20 (s, 3H, 2I-COCH 3 ), 3.60 (m, IH, 16-H) , 4.90 - 4.38 (m, 3H, 20-CH 2 O and 11-H) , 6.04 (s, IH, 4-H) , 6.29 (dd, IH, J=10 and 2Hz, 2-H) , 7.23 (d, IH, J=10Hz, 1-H) . Example 17
To a solution of the product of Example 15 (500 mg, 1.35 mmole) in dimethylsulfoxide (15 ml) was added 20% HC1 (10 ml) . After stirring for 5 hrs at room temperature, the mixture was diluted with dichloromethane (250 ml) and washed with 0.1% NaCHθ 3 (100 ml) and water (100 ml) , followed by evaporating the organics to dryness. Recrystallization from acetone gave 203 mg of white prisms of 16a-cyano-llβ , 21-dihydroxy-l, 4-pregnadiene-3 , 20-dione (40.63%), mp 230-234°C; Rf = 0.52 (CHCl 3 /MeOH = 85:15); Η - NMR (CDCI 3 ) δ 0.90 (s, 3H, I3-CH 3 ) , 0.44 (s, 3H, 10-CH 3 ) , 3.63 (m, IH, 16-H) , 4.25 (s(distorted) , 2H, 20-CH 2 O) , 4.48 (m, IH, 11-H) , 6.04 (S, IH, 4-H) , 6.29 (dd, IH, J=10 and 2Hz, 2-H) , 7.21 (d, IH, J=10Hz, 1-H) . Example 18
The product of Example 16 (960 mg) was converted to the corresponding 21-tetrahydropyranyl compound using the procedure of Example 13. Recrystallized from methanol gave white prisms of 21-tetrahydropyranyl-16a-cyano-ll , 21-dihydroxy-l, 4-pregnadiene- 3,20-dione.
Example 19
To a 200 mg of the product of Example 17 in methanol (30 ml) , IN aOH (3 ml) was added and refluxed for 2 hours. After diluting with water (500 ml) , unreacted and neutral compounds were extracted away with' ethylacetate. The aqueous solution was acidified to pH 1.5 with 0.5N HC1, the carboxylic acids were extracted with ethyl¬ acetate, and washed with water. After drying on anhydrous sodium sulfate, the organic were evaporated to dryness to give 21-tetra- hydropyranyl-16a-carboxy-llβ-hydroxy-l, 4-pregnadiene-3 , 20-dione and the corresponding 16-β-carboxy compound. To the solution of these compounds in methanol, a small amount of H 2 SOtj was added and refluxed for 3 hours. After the usual work-up, 50 mg of white solid was obtained. This solid was subjected to column chroma¬ tography using benzene/ethylacetate 1:1 as a mobile phase. After combining the corresponding fractions, the organics were evaporized to give dryness to give 16 (a and β)-methoxycarbonyl-llβ , 21- dihydroxy-1, 4-pregnadiene-3, 20-dione. in NMR, this product was determined to be a mixture of 16a- and 16β- ketol derivatives. Based on the NMR peak intensity, a/β ratio is 1/1.7 1H-NMR(CDC1 3 ) , δ: 0.98 (13-CH 3 for β) , 1.23 (13-CH 3 for a), 1.42 (10-CH 3 for a), 1.44 (IO-CH 3 for β), 3.65 (s, COOCH 3 for β) 3.67 (s, COOCH 3 for a), 4.21 (m, 2H, 20-CH 2 O- ), 4.43 (m, 11-H) , 601(4-H), 6.26(2-1-1), 7.2(1-H) . Example 20
To a solution of the compounds of Example 18 in pyridine there was added acetic anhydride. After 3 hours at room tempera¬ ture, the reaction mixture was diluted ' with ethylacetate and washed thoroughly with 0.5N HCl and water. Evaporation of the organic solvent gave a white solid with a yield of 75%. Pure forms of 21-acetoxy-16 (a and β)-methoxycarbonyl-llβ, 21-dihydroxy-l, 4- pregnadiene-3,20-dione was obtained by preparative thin layer chromatography with benzene/ethylacetate as developing solvent. 'H-NMR for XVIIa (CDC1 3 ) δ: 1.23(s, 3H, 13-CH 3 ) 1.42 (s, 3H, IO-CH 3 ), 3.67(s, 3H, I6-COOCH 3 ), 4.21(m, 2H, 20-CH 2 O- ), 4.43(m, IH, 11-1-1) ,
6-:01(s, IH, 4-H) , 6.26(dd, IH, J=10 and 2-Hz, 2-H) , 7.20(d, IH, J=10Hz, 1-H) .
Example 21
To a solution of the product of Example 16 in methanol, 20% HC1 was added. After reacting at 60°C for 6 hours, the reaction mixture was diluted with 500 ml of H 2 0 and extracted with ethylacetate. The resulting organic extract was evaporated to dryness. The product was purified on a silica gel column using σhloroform/methanol (85:15, V/V) . The yield obtained from methanol crystallization was 24%. The product was 21-acetoxy- 16a-aminocarbonyl-llβ-hydroxy-l,4-pregnadiene-3,20-dione. Example 22
The intermediate products of- Example 18 having 16a-or β- carboxy were converted to the corresponding 16α-or β-bromo by treatment with hydrogen bromide or copper bromide. To a solution of the 16a-or β-bromo product in methanol (70 mg) was slowly added 2N NaOH solution (4 ml) . After stirring for 3 hours at room tem¬ perature, the reaction mixture was diluted with IL. water, followed by acidification to pH 1.5 with 0.1N HC1. The reaction mixture was extracted with ethylacetate. After drying the organic phase with anhydrous sodium sulfate, the solvent was evaporated to dryness to produce solid llβ, 17 (a and β) , 21-trihydroxy-16α-carboxy-l, 4- pregnadiene-3,20-dione. This product was esterified with ether/ diazomethane. The product was crystallized from methanol with a yield of 35% to produce 16a-methoxycarbonyl-llβ , 17 (α and β) , 21- trihydroxy-1, 4-pregnadiene-3,20-dione. 'H-NMR (CDC1 3 ) δ *. 1.23 (s, 3H, I3-CH 3 ), 1.42(s, ' 3H, IO-CH 3 ) , 3.67(s, 3H, 16-COOCH 3 ) , 4.21 (m, 2H, 20-CH 2 O-) , 4.43(m, IH, 11-H) , 6.01(s, IH, 4-H) , 6.26 (dd, IH, 2-H) , 7.20(d, IH, 1-H) . Example 23
To a solution of methyl-llβ , 17a, 20α— trihydroxy-3-oxo-l, 4-pregnadien-21-oate (lg) in methanol (70 ml) was slowly added 2N sodium hydroxide solution (4 ml) . After stirring for 3 hours at room temperature, the reaction mixture was diluted with water (IL) , followed by acidification to pH 1.5 with 0.1N HC1. The acidified reaction mixture was extracted with ethylacetate. After drying the extract on anhydrous sodium sulfate, the solvent was evaporated to dryness. Recrystallization from methanol gave 740 mg of prisms
of llβ, 17, 20a-trihydroxy-3-oxo-l, 4-pregnadien-21-oic acid mp= 213-214°C; [a] D + 34.7°; λmax 224 nm (ε = 17,000); 1R 3440 (OH) 3250 and 1735 cm "1 (COOH); 'H-NMR (Me 2 SO,d 6 ) δ 1.03(s, 3-H, 13-CH 3 ), 1.39(s, 3H, IO-CH 3 ), 4.19(s, IH, 20-H) , 4.60(m, IH, 11-H) , 5.9(s, IH, 4-H) , 6.13(dd, IH, J=10 and 2 Hz, 2H), ' 7.31(d, IH, J=10Hz, 1-H) , 8.30(s, IH, 20-COOH) ; Anal. (C 21 H 28 O s . CH 3 OH) C, H.
If the 20β-starting material is used and is saponified as above, and recrystallized from aqueous methanol, there is obtained 608 mg of-prisms of llβ, 17, 20β-trihydroxy-3-oxo-l, 4-pregnadien- 21-oic acid mp= 165-167°C; [al D + 18.1°; λmax 243nm (ε = 15,200); 1R 3430 and 1738 cm "1 (COOH); 'H-NMR (Me 2 SO,d 6 ) δ 1.06(s, 3H, 13-CH 3 ), 1.42(s, 3H, IO-CH 3 ), 4.15(s, IH, 20-H) , 4.54(m, IH, 11-H) , 5.89 (s, IH, 4-H) , 6.15(dd, IH, j=10 and 2Hz, 2-H) , 7.33(d, IH, J=10Hz, 1-H) , 8.31(s, IH, 20-COOH) ; Anal. (C 21 H 28 O s - H 2 0) C,H. Example 24
To a ice-cooled solution of either of the 20a- or 20β- hydroxy products of Example 22 (500 mg) in tetrahydrofuran (3 ml) and CH 2 Cl 2 were added N, N'-dicyclohexylcarbodiniimide (270 mg) and 1-hydroxybenzotriazole (310 mg) in tetrahydrofuran (7 ml) . After stirring for 24 hours at 4°C, the reaction mixture was filtered to remove excess N, N'-dicyclohexylcarbodii ide and a primary amine, e.g., methylamine, ethylamine, n-propylamine or benzylamine, (0.8 mmole) was added to the filtrate. Stirring was continued for 24 hours at 4°C. The solvent was evaporated to dryness. After dissolving the residue with 7 ml chloroform/ methanol (9:1), the solution was placed on silica gel column (2 x 60 cm) and eluted with chloroform/methanol (9:1) or (95.5) in case of benzylamine. Fractions corresponding to each amine were combined and evaporated to dryness. Recrystallization from various solvent mixture gave pure products in various yields (37-85%) . The products were 21-(methyl, ethyl, n-propyl, or benzyl) amids-llβ, 17, 20ς-trihydroxy-1, 4-pregnadien -3, 20-dione. The physical data are as follows:
0a-OH-20 -Methylamide, 'H-NMR (Me 2 SO,d s ) δ 0.99(s, 3H, 13-CH 3 ), 1.37(s, 3H, IO-CH3), 2.59(d, 3H, J=4, 5Hz, -NH-CH 3 ) , 3.78(d, IH, J=7Hz, 20-H) , 4.16(m, IH, 11-H) 5.89(s, IH, 4-H) , 6.12(dd, IH, J=10 and 2Hz, 2-H) , 7.30(d, IH, J=10Hz, 1-H) , 7.77(m, IH, -NH-) 0β-OH-20 -Methylamide, 'H-NMR(Me 2 SO,d 6 ) δ 0.99(s, 3H, 13-CH 3 ), 1.38(s, 3H, IO-CH 3 ), 2.57(d, 3H, J=4.5Hz, -NH-CH 3 ) , 3.93 (d, IH, J=8Hz, 20-H) , 4.18(m, IH, 11-H) , 5.88(s, IH, 4-H) , 6.13 (dd, IH, J=10 and 2Hz, 2-H) , 7.30(d, IH, J=10Hz, 1-H) , 7.61(m, IH, -NH-) . a- OH -20 -Ethylamide, 'H-NMR (Me 2 SO,d 6 ) δ 1-1.07(m, 6H, 13-CH 3 and NHCH 2 -CH 3 ), 1.38(s, 3H, IO-CH 3 ), 3.15(m, 2H, NH-CH 2 ), 3.78(d, IH, J=5Hz, 20-H) , 4.16(m, IH, 11-H) , 5.89(s, IH, 4-H) , 6.12(dd, IH, J=10 and 2Hz, 2-H) , 7.29(d, IH, J=10Hz,- 1-H) , 7.77(m, IH, -NH-) β-OH-20 -Ethylamide, 'H-NMR (CDCI 3 ), δ 1.14-1.20(m, 6H, 13-CH 3 and NHCH 2 -CH 3 ), 1.47(S, 3H, 10-CH 3 ), 3.34(m, 2H, NH-CH 2 -) , 4.09 (d, IH, J=5Hz, 20H) , 4.43(m, IH, 11-H) , 6.01(s, IH, 4-H) , 6.19(m, IH, -NH-) , 6.24(dd, IH, J=10 and 2Hz, 2-H) , 7.26 (d, iH, J=10Hz, 1-H) . a-OH-20.-propylamide, 'H-NMR (CDC1 3 ) . δ U.94(t, 3H, J=6Hz, -NH (CH 2 ) 2 -CH 3 ), 1.15(s, 3H, U-CH 3 ), 1.46(s, 3H, 10-CH 3 ), 3.2(m, 2H, -NH-CH 2 -C 2 H 5 ) , 4.06(s, IH, 20-H) , 4.39(m, IH, il-H) , 6.0(s, IH, 4-H) , 6.25(dd, IH, J=10 and 2Hz, 2-H) , 6.9(m, IH, -NH-) , 7.31(d, IH, J=10Hz, 1-H) . β-OH-20 -propylamide, 'H-NMR (CDC1 . , δ 0.94(t, 3H, J=6 Hz, -NH- (CH 2 ) 2 -CH 3 ), 1..14(s, 3H, 13-CH 3 ), 1.46(s, 3H, IO-CH 3 ) , 3.26(m, H, -NH-CH 2 - CH 5 ), 4.10(d, IH, J=Hz, 20-H) , 4.44 (m, IH, 11-H) , 6.02(s, lH, 4-H) , 6.19(m, IH, -NH-) , 6.26 (dd, IH, J=10 and 2Hz, 2-H) , 7.26(d, IH, J=lϋ Hz, 1-H) a-OH-20 -benzylamide, 'H-NMR (Me 2 SO,d s ) , l.04(s, 3H, 13-CH 3 ), 1.37(s, 3H, 10-CH 3 ), 3.91(d, IH, J=5 Hz, 20-H) , 4.14 (m, IH, il-H), 4.2(J-4.46(m, H, -CH 2 -«), 5.89(s, IH, 4-H) , 6.13(dd, IH, J=10 and 2Hz, 2-H) , 7.18-7.34 (m, 6H, and 1-H) , 8.08(m, IH, -NH-)
20a-OH-2Q- ben2 y lamide ' 'H-NMR (Me 2 SO,d 6 ) δ 1.02(s, 3H, 13-CH 3 ) , 1.39(s, 3H, IO-CH 3 ), 4.01(d, IH, J=8 Hz, 20-H) , ' 4.15-4.32 (m, 3H, -CH 2 -Φ, and 11-H) , 5.89(s, IH, 4-H) , 6.13(dd, IH, J=10 and 2Hz, 2-H) , 7.22-7.36(m, 6H, Φ and 1-H) , 8.08 (m, IH, -NH-) Example 25
To a ice-cooled solution of 21-acetoxy-llβ , 17a-dihydroxy- 1, 4-pregnadiene-3,20-dione (7g) in methanol (40 ml), NaBHt, (0.6g) was added slowly. After stirring at 0°C for 30 minutes 0.5N acetic acid solution (350 ml) was added. After extracting with ethylacetate the organic layer was washed with water twice and dried on anhydrous sodium sulfate. The solvent was evaporated under vacuum to give white solid, which was dissolved in small amount of methanol. The above solution was placed on silica gel column and eluted with chloroform/methanol (9:1) . The eluate which is more polar product, followed by crystallization from acetone gave 21-acetoxy-llβ , 17a, 20 α -trihydroxy 1, 4-pregnadiene-3-one (230 mg) which is identical to "Delta-trial acetate". Major portion gave 21-acetoxy-llβ , 17a, 20β-trihydroxy-l, 4-pregnadiene-3-one (4.9g) from acetone. Example 26
Normal saponification treatment of each of the products of Example 24 with 4N NaOH in methanol afforded crude products. Re¬ crystallization of the 20a-product from acetone/methanol gave llβ, 17 α , 20a, 21-tetrahydroxy-l,4-pregnadiene-3-one as needles. Pure llβ, 17 α , 20β, 21-tetrahydroxy-l,4-pregnadiene-3-one was obtained by recrystallization from acetone/ethylacetate. Example 27
The products of Example 24 were treated separately with perchloric acid in acetone to produce respectively, 21-acetoxy- llβ-hydroxy-17, 20 (a or β) acetonide-l,4-pregnadiene-3-one. The a- acetonide product exhibited a m.p. of 213-215°C and the β- acetonide product exhibited a m.p. of _.10 U -213°C. Example 28
To a solution of methyl-llβ, 17 α , 20 (a or β) -trihydroxy-3- oxo-1,4-pregnadien--.l-oate(100 mg) in 10 ml methanol, 82 mg NaBH t , was added slowly. After rerluxing for 20 minutes, 0.5N acetic acid
solution was added. Extraction w th ethylacetate and evapora¬ tion gave a white solid. After separation with silica gel column by chloroform/methanol (9:1), the solvent was evaporated. Recrystallization from methanol gave, respectively, llβ, 17a, 20(a or β) , 21-tetrahydroxy-l,4-pregnadiene-3-one, the same products as produced in Example 25. Example 29
To a solution of the 21-N-propylamide product of Example 23 (300 mg) in 30 ml of tetrahydrofuran a suspension of MπO 2 (30 mg) in 200 ml of tetrahydrofuran was added. The mixture was refluxed overnight with vigorous stirring. The reaction mixture was fil¬ tered and the filtrate was evaporated to dryness. The crude product was subjected to silica gel column chromatography using chloroform/methanol (95.5, v/v) , as the eluting solvent. The steroid was crystallized from methanol in the amount of 10 mg and was 21-N-propylamide-ll , 17α-dihydroxy-l, 4-prednadiene- 3, 20-dione. NMR (CDC1 3 ) δ: 0.8(3H, t, propyl CH 3 ) , 1.1(3H, s, 10-CHg), 1.4(3H, s, 13-CH 3 ) 6.65(2H, m, NH-CH 2 -C 2 H 5 ) , 4.4 (IH, m, 11-H) , 5.95(1H, t, C^-H) , 6.2(1H, d of d, C 2 -H) 6.75 (IH, b, N-H) , 7.17(1H, d of d, C -H) . Example 30
Compounds of this invention were tested for pharmacological evaluation. The following procedures were employed. Adult male Sprague-Dawley rats weighing 120-140 g were maintained on standard laboratory chow with water ad_ libitum and kept under controlled condition for one week prior to their use. Cotton pellets weighing 35 + 1 mg cut from dental rolls were impregnated with steroid sol¬ ution in acetone (0.2 or 0.4 ml) and the solvent was removed by evaporation. The cotton pellets were subsequently injected with 0.2 ml aqueous solution of antibiotics (1 mg penicillin G and 1.3 mg dihydrostreptomycin/ml) . Two cotton pellets were implanted s.c, one in each axilla of the rat under light ether aneasthesia. Cotton pellets containing only the antibiotic solution were sim¬ ilarly implanted in the control rats. Seven days later, the animals were sacrified and the two pellets, with their adhering granulomas, were removed, dried for 58 hours in an oven at 60°C
and weighed. The increment in dry weight (difference between the initial and final pellets weight) is taken as a measure of granuloma formation. The adrenal, thymus and final body weight were also recorded. The adrenal and thymus weights were expressed as relative weights (mg tissue/100 g body weight) .
For local and systemic effects of steroids, one cotton pellet impregnated with tested steroid was implanted in one axilla of the rat and the other cotton pellet containing only the antibiotic was implanted in the other axilla.
The results are shown in Table 1 where systemic and local effects are evaluated. Table 2 reports local activity of certain compounds. All results are compared to a control with no steroid used and to tests with prednisolone as the standard steroid.
TABLE I
DOSAGE DRY WT. OF GRANULOMA RELATIVE WEIGHT
COMPOUND OF mg/cotton GRANULOMA INHIBITION mg EXAMPLE NO. pellet mg. % THYMUS ADRENAL
None(Control) 0.0 40.4±2.6 22.9+9.7 13.9+2.9
Prednisolone 1.0 24.1±1.3 40.5 202.5+13.7 14.8+0.4 0.0 24.7±1.7 38.8
Prednisolone 2.0 17.9+0.5 5b.7 83.0+4.0 15.7+0.3 0.0 26.5+7.0 34.5
4 (2d cmpd) 2.0 31.8+1.6 21.4 228.1+9.6 15.3+1.4 0.0 45.2+7.2 -11.9
16 2.0 24.7±0.8 38.8 229.8+17.8 14.8+0.5 0.0 31.3±3.7 22.6
17 2.0 25.9±2.6 35.6 193.9±10.4 16.2+0.8 0.0 34.0±3.7 15.9
20(16acmpd) 2.0 33.5+1.4 17.3 228.9±17.5 14.7+0.9 0.0 47.6+5.1 -17.6
20(16βcmpd) 1.0 24.1+1.3 40.3 250.9+20.4 14.1±0.5 41.2±4.2 - 1.9
15(1st cmpd) 2.0 25.0+2.8 36.1 227.6±9.0 14.2+0.6 0.0 31.6±2.3 21.9
15(2d cmpd) 2.0 35.ε±3.1 12.3 240.2±22.9 14.3+0.9 0.0 38.3+2.8 5.3
22 2.0 20.2±1.4 50.0 242 + 6.0 12.6±0.8 0.0 39.9±5.5 1.2
Several of the ' compounds in Table 1 show high values for local granuloma inhibition (substantially equivalent to that of prednisolone) while not suppressing systemic granu¬ loma formation nor decreasing the weight of the thymus and adrenal glands to the large extent caused by prednisolone.
TABLE 2
DOSAGE GRANULOMA
COMPOUND OF mg/cotton INHIBITION RELATIVE WEIGH. EXAMPLE NO. pellet % THYMUS ADREΪ IAL
Control 0.0 209.9 ± 13.2 14.2 ± 1.0
Prednisolone 2.5 58.9 84.4 + 15.7 15.0 + 0.6
Prednisolone 1.5 50.9 136.2 + 3.9 15.4 + 1.4
15 (2d cmpd) 2.5 41.8 270.7 + 16.9 13.8 + 0.7
24(N-propyl cmpd) 2.5 57.8 215.0 + 13.4 • 12.8 + 1.0
19 2.5 55.9 206.5 + 14.2 11.1 + 0.6
22 2.5 66.0 101.0 + 6.2 12.5 ± 0.7
7 2.5 62.0 292.8 4- 21.0 14.4 + 0.8
8 1.0 43.4 320.1 + 19.1 16.1 + 1.4
10 1.5 63.3 282.9 + 18.8 15.0 + 0.6
It is apparent from the above results that all of the compounds provide equal or better activity than prednisolone in suppressing granuloma growth and also in maintaining the relative weights of the thymus and adrenal glands.
While the invention has been described with respect to certain specific embodiments, it w ll be appreciated that many modifications and changes may be made by those skilled in the art without departing from the spirit of the invention. It is intended, therefore, by the appended claims to cover all such modifications and changes as fall within the true spirit and scope of the invention.