Title:
ANTI-L1CAM ANTIBODY OR ANTIGEN-BINDING FRAGMENT THEREOF AND CHIMERIC ANTIGEN RECEPTOR COMPRISING SAME
Document Type and Number:
WIPO Patent Application WO/2020/080908
Kind Code:
A1
Abstract:
The present invention relates to an anti-L1CAM antibody specifically binding to L1CAM antigen or an antigen-binding fragment thereof, a chimeric antigen receptor comprising same, and uses thereof. The anti-L1CAM antibody or the antigen-binding fragment of the present invention is excellent in specificity and affinity to L1CAM and thus may be used in the treatment and diagnosis of cancers related to high expression of L1CAM and diseases related to inflammatory disorders. In particular, when the chimeric antigen receptor comprising the anti-L1CAM antibody of the present invention is expressed in effector cells such as T lymphocytes, the chimeric antigen receptor may be effectively used as immunotherapy for cancers related to L1CAM and inflammatory disorders.
Inventors:
CHAE JIN-A (KR)
JEONG JAE-GYUN (KR)
KIM DAE YOUNG (KR)
KIM YU JUNG (KR)
YOO BIN (KR)
JEONG JAE-GYUN (KR)
KIM DAE YOUNG (KR)
KIM YU JUNG (KR)
YOO BIN (KR)
Application Number:
PCT/KR2019/013820
Publication Date:
April 23, 2020
Filing Date:
October 21, 2019
Export Citation:
Assignee:
HELIXMITH CO LTD (KR)
International Classes:
C07K16/28; A61P1/00; A61P29/00; A61P35/00; C07K14/705; C12N5/0783; C12N15/86
Foreign References:
| US20150038684A1 | 2015-02-05 | |||
| KR20140066101A | 2014-05-30 | |||
| KR20100064985A | 2010-06-15 | |||
| KR20180125538A | 2018-11-23 | |||
| US4554101A | 1985-11-19 | |||
| US7049143B2 | 2006-05-23 | |||
| US6451595B1 | 2002-09-17 |
Other References:
HONG, H.: "L1 cell adhesion molecule-specific chimeric antigen receptor- redirected human T cells exhibit specific and efficient antitumor activity against human ovarian cancer in mice", PLOS ONE, vol. 11, no. 1, 13 January 2016 (2016-01-13), pages 1 - 18, XP055703051
SCHAFER, H.: "TGF-beta1-dependent L1CAM expression has an essential role in macrophage-induced apoptosis resistance and cell migration of human intestinal epithelial cells", ONCOGENE, 20 February 2012 (2012-02-20), pages 180 - 189, XP055703082
CHO, S.: "Generation, characterization and preclinical studies of a human anti-LlCAM monoclonal antibody that cross-reacts with rodent L1CAM", MABS, 2016, pages 414 - 425, XP055703160
"Biomedical Technology Development Project (Next-Generation Bio) Immunity Mechanism Control Technology Development", 1 May 2016, SEOUL NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, article "Research on Multifunctional Fusion T Cell Therapy Using Chimeric Antigen Receptor and B Cells"
HAO HONG: "L1 Cell Adhesion Molecule-Specific Chimeric Antigen Receptor-Redirected Human T Cells Exhibit Specific and Efficient Antitumor Activity against Human Ovarian Cancer in Mice", PLOS ONE, vol. 11, no. 1, 2016, pages e0146885, XP055703051, DOI: 10.1371/journal.pone.0146885
WONSIEWICZ, M. J.: "Cellular and Molecular Immunology", vol. 45, 1991, W. B. SAUNDERS CO, pages: 41 - 50
NISONOFF, A.: "Introduction to Molecular Immunology", vol. 4, 1984, SINAUER ASSOCIATES, INC., pages: 45 - 65
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1987, U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
H. NEURATHR. L. HILL: "The Proteins", 1979, ACADEMIC PRESS
SCHEIT: "Nucleotide Analogs", 1980, JOHN WILEY
UHLMANPEYMAN, CHEMICAL REVIEWS, vol. 90, 1990, pages 543 - 584
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIO., vol. 48, 1970, pages 443
PEARSONLIPMAN, METHODS IN MOL. BIOL., vol. 24, 1988, pages 307 - 31
HIGGINSSHARP, GENE, vol. 73, 1988, pages 237 - 44
HIGGINSSHARP, CABIOS, vol. 5, 1989, pages 151 - 3
CORPET ET AL., NUC. ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., COMP. APPL. BIOSCI., vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 31
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
ROSENBERG ET AL., NEW ENG. J. OF MED., vol. 319, 1988, pages 1676
See also references of EP 3868783A4
SCHAFER, H.: "TGF-beta1-dependent L1CAM expression has an essential role in macrophage-induced apoptosis resistance and cell migration of human intestinal epithelial cells", ONCOGENE, 20 February 2012 (2012-02-20), pages 180 - 189, XP055703082
CHO, S.: "Generation, characterization and preclinical studies of a human anti-LlCAM monoclonal antibody that cross-reacts with rodent L1CAM", MABS, 2016, pages 414 - 425, XP055703160
"Biomedical Technology Development Project (Next-Generation Bio) Immunity Mechanism Control Technology Development", 1 May 2016, SEOUL NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, article "Research on Multifunctional Fusion T Cell Therapy Using Chimeric Antigen Receptor and B Cells"
HAO HONG: "L1 Cell Adhesion Molecule-Specific Chimeric Antigen Receptor-Redirected Human T Cells Exhibit Specific and Efficient Antitumor Activity against Human Ovarian Cancer in Mice", PLOS ONE, vol. 11, no. 1, 2016, pages e0146885, XP055703051, DOI: 10.1371/journal.pone.0146885
WONSIEWICZ, M. J.: "Cellular and Molecular Immunology", vol. 45, 1991, W. B. SAUNDERS CO, pages: 41 - 50
NISONOFF, A.: "Introduction to Molecular Immunology", vol. 4, 1984, SINAUER ASSOCIATES, INC., pages: 45 - 65
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1987, U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
H. NEURATHR. L. HILL: "The Proteins", 1979, ACADEMIC PRESS
SCHEIT: "Nucleotide Analogs", 1980, JOHN WILEY
UHLMANPEYMAN, CHEMICAL REVIEWS, vol. 90, 1990, pages 543 - 584
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIO., vol. 48, 1970, pages 443
PEARSONLIPMAN, METHODS IN MOL. BIOL., vol. 24, 1988, pages 307 - 31
HIGGINSSHARP, GENE, vol. 73, 1988, pages 237 - 44
HIGGINSSHARP, CABIOS, vol. 5, 1989, pages 151 - 3
CORPET ET AL., NUC. ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., COMP. APPL. BIOSCI., vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 31
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
ROSENBERG ET AL., NEW ENG. J. OF MED., vol. 319, 1988, pages 1676
See also references of EP 3868783A4
Attorney, Agent or Firm:
YOON, Dae Woong (KR)
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