60/279,949, filed March 29,2001.

FIELD OF THE INVENTION The present invention relates to anti- bacterial compositions, like personal care composi- tions, having high antibacterial effectiveness and excellent esthetic properties, such as foam genera- tion, foam stability, and a capability of imparting skin care properties to cleansed skin. More partic- ularly, the present invention relates to antibac- terial compositions comprising an antibacterial agent, a surfactant, a hydrotrope, a hydric solvent, esthetics-enhancing ingredients, and optional skin care ingredients, and that provide a substantial reduction, e. g., greater than 99%, in Gram positive and Gram negative bacteria populations within one minute.

BACKGROUND OF THE INVENTION Antibacterial personal care compositions are known in the art. Especially useful are anti- bacterial cleansing compositions, which typically are used to cleanse the skin and to destroy bacteria

and other microorganisms present on the skin, es- pecially the hands, arms, and face of the user.

Antibacterial compositions are used, for example, in the health care industry, food service industry, meat processing industry, and in the private sector by individual consumers. The wide- spread use of antibacterial compositions indicates the importance consumers place on controlling bac- teria and other microorganism populations on skin.

It is important, however, that antibacterial com- positions provide a substantial and broad spectrum reduction in microorganism populations quickly and without problems associated with toxicity and skin irritation.

In particular, antibacterial cleansing compositions typically contain an active antibac- terial agent, a surfactant, and various other ingre- dients, for example, dyes, fragrances, pH adjusters, thickeners, and the like, in an aqueous carrier.

Several different classes of antibacterial agents have been used in antibacterial cleansing composi- tions. Examples of antibacterial agents include a bisguanidine (e. g., chlorhexidine digluconate), diphenyl compounds, benzyl alcohols, trihalocarban- ilides, quaternary ammonium compounds, ethoxylated phenols, and phenolic compounds, such as halo-sub- stituted phenolic compounds, like PCMX (i. e., p- chloro-m-xylenol) and triclosan (i. e., 2,4,4'-tri- chloro-2'hydroxy-diphenylether). Present-day anti- microbial compositions based on such antibacterial agents exhibit a wide range of antibacterial activity, ranging from low to high, depending on the

microorganism to be controlled and the particular antibacterial composition.

Most commercial antibacterial composi- tions, however, generally offer a low to moderate antibacterial activity. Antibacterial activity is assessed against a broad spectrum of microorganisms, including both Gram positive and Gram negative microorganisms. The log reduction, or alternatively the percent reduction, in bacterial populations provided by the antibacterial composition correlates to antibacterial activity. A log reduction of 3-5 is most preferred, a 1-3 reduction is preferred, whereas a log reduction of less than 1 is least pre- ferred, for a particular contact time, generally ranging from 15 seconds to 5 minutes. Thus, a high- ly preferred antibacterial composition exhibits a 3- 5 log reduction against a broad spectrum of micro- organisms in a short contact time. Prior disclo- sures illustrate attempts to provide such antibac- terial compositions, which, to date, do not provide the rapid, broad range control of microorganisms desired by consumers.

It should be noted that high log reduc- tions have been achieved at pH values of 4 and 9, but such log reductions are attributed at least in part to these relatively extreme pH values. Compo- sitions having such pH values can irritate the skin and other surfaces, and, therefore, typically are avoided. It has been difficult to impossible to achieve a high log reduction using an antibacterial composition having a neutral pH of about 5 to about 8, and especially about 6 to about 8.

However, highly efficacious antibacterial compositions suffer in comparison to regular (i. e., nonantibacterial) personal care compositions with respect to acceptable consumer properties, especial- ly foam characteristics and imparting skin care properties, such as skin conditioning. It also is difficult to provide phase stable, highly effica- cious antibacterial compositions having consumer- acceptable esthetics. Further, present-day antibac- terial personal care compositions do not provide an effective antibacterial activity, especially against pathogenic Gram negative bacteria. Thus, a need exists for phase stable, efficacious antibacterial personal care compositions containing skin care ingredients, and that further are consumer accept- able.

An example of patents and published appli- cations disclosing compositions comprising triclo- san, surfactants, solvents, chelating agents, thick- eners, buffering agents, and water is WO 98/01110.

WO 98/01110 is directed to reducing skin irritation by employing a reduced amount of surfactant.

Fendler et al. U. S. Patent No. 5,635,462 discloses compositions comprising PCMX and selected surfactants. The compositions disclosed therein are devoid of anionic surfactants and nonionic surfac- tants.

WO 97/46218 and WO 96/06152 disclose com- positions based on triclosan, organic acids or salts, hydrotropes, and hydric solvents.

EP 0 505 935 discloses compositions con- taining PCMX in combination with nonionic and an-

ionic surfactants, particularly nonionic block co- polymer surfactants.

WO 95/32705 discloses a mild surfactant combination that can be combined with antibacterial compounds, like triclosan.

WO 95/09605 discloses antibacterial comp- ositions containing anionic surfactants and alkyl- polyglycoside surfactants.

WO 98/55096 discloses antimicrobial wipes having a porous sheet impregnated with an antibac- terial composition containing an active antimicrobi- al agent, an anionic surfactant, an acid, and water, wherein the composition has a pH of about 3.0 to about 6.0.

Glenn, Jr. et al. U. S. Patent No.

5,885,948 discloses a stress stable, lathering skin cleansing composition containing about one to 30 parts lipid skin moisturizing agents.

Beerse et al. U. S. Patent Nos. 5,968,539; 6,106,851; and 6,113,933 disclose antibacterial compositions having a pH of about 3 to about 6. The compositions contain an antibacterial agent, an an- ionic surfactant, and a proton donor.

N. A. Allawala et al., J. Amer. Pharm.

Assoc.--Sci. Ed., Vol. XLII, no. 5, pp. 267-275, (1953) discusses the antibacterial activity of active antibacterial agents in combination with surfactants.

A. G. Mitchell, J. Pharm. Pharmacol., Vol.

16, pp. 533-537, (1964) discloses compositions con- taining PCMX and a nonionic surfactant that exhibit antibacterial activity. The compositions disclosed in the Mitchell publication exhibit antibacterial

activity in at least 47 minutes contact time, thus the compositions are not highly effective.

Prior disclosures have not addressed the issue of providing an antibacterial composition that (a) affords an effective, fast, and broad spectrum control of bacteria at a neutral pH of about 5 to about 8, and especially at about 6 to about 8, (b) is phase stable, (c) exhibits excellent esthetic properties, such as a stable, copious foam genera- tion, and (d) imparts skin care properties to cleansed skin. In addition to the above, prior disclosures also have not addressed providing a composition of sufficiently low viscosity for use with a self-foaming pump.

An efficacious antibacterial composition has been difficult to achieve because of the prop- erties of the antibacterial agents and the effects of a surfactant, a hydrotrope, and a hydric solvent on an antibacterial agent. One such efficacious antibacterial composition is discussed in Taylor et al. U. S. Patent No. 6,107,261, incorporated herein by reference. This patent discloses a highly effi- cacious antibacterial composition against Gram nega- tive and Gram positive bacteria, and containing a high percent (at least 25%) saturation of a phenolic antibacterial agent. The positive effects of a higher percent of saturation of antibacterial agent is fully discussed in U. S. Patent No. 6,107,261.

A need now exists for an antibacterial composition that is highly efficacious against a broad spectrum of Gram positive and Gram negative bacteria in a short time period, wherein the anti- bacterial activity is attributed primarily, or

solely, to the presence of the active antibacterial agent in the composition, and has consumer-accept- able esthetic properties with respect to phase stability, feel, foam generation and stability, and imparting skin care properties. The present inven- tion is directed to such efficacious and esthet- ically pleasing antibacterial compositions.

The development of such compositions is difficult because of factors such as a) the need for a high antimicrobial efficacy even in the presence of esthetic enhancing and skin care additives, b) the need to maintain a relatively high % saturation of the antibacterial agent, and c) the difficulty in formulating a high-foaming composition in the pres- ence of significant amounts of a hydrotrope and hydric solvent. Unlike present-day commercial com- positions and compositions disclosed in the prior art, the variety, type, and amounts of esthetic en- hancing and skin care additives that can be incor- porated in the present compositions are varied and unexpected, and a high percent saturation of anti- bacterial agent can be maintained.

In addition, antibacterial composition viscosity also is critical for particular applica- tions. For example, a preferred method of using the composition is with a self-foaming pump. If the viscosity of the composition is too high (e. g., greater than about 50 centipoise), the composition cannot be pumped through a preferred foaming device.

Finally, foam generation and stability also are important for consumer acceptability. Compositions of the present invention exhibit excellent viscos- ity, enhanced foam volume, creaminess, and slip dur-

ing human use tests. This is especially important for application of the antibacterial composition to dry hands through a foaming pump, followed by about 30 seconds lathering, and completed by rinsing with water. This type of application provides the high- est antibacterial effect.

SUMMARY OF THE INVENTION The present invention is directed to anti- bacterial compositions that provide a substantial reduction in Gram positive and Gram negative bac- teria in less than about one minute. More partic- ularly, the present invention is directed to anti- microbial compositions containing an active anti- bacterial agent, a surfactant, and water, in addi- tion to ingredients such as emollients, humectants, and foam stabilizers to impart esthetics to the composition and skin care properties to cleansed skin. The antibacterial agent is present in the composition in an amount of at least 25% of satura- tion, when measured at room temperature. The pres- ent antimicrobial compositions are phase stable, and can be designed to have a viscosity suitable for a variety of end uses, including a composition for use with a self-foaming pump and a composition that is applied to the skin neat, lathered to cleanse the skin and kill bacteria, followed by rinsing from the skin.

Accordingly, one aspect of the present in- vention is to provide an antibacterial composition, wherein the composition comprises:

(a) about 0. 001% to about 10%, by weight, of an antimicrobial agent ; (b) about 0. 1% to about 40%, by weight, of a surfactant selected from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an ampholytic surfactant, and mixtures thereof; (c) about 1% to about 40%, by weight, of a hydrotrope; (d) about 1% to about 25%, by weight, of a water-soluble hydric solvent; and (e) 0% to about 5%, by weight, of a skin care agent; (f) 0% to about 5%, by weight, of a foam stabilizer; (g) 0% to about 5%, by weight, of a humectant; and (h) water, wherein the composition contains at least one of (e), (f), and (g), and wherein the antimicro- bial agent is present in the composition in an amount of at least 25% of saturation concentration, when measured at room temperature.

Another aspect of the present invention is to provide an antibacterial composition that ex- hibits a log reduction against Gram positive bac- teria (i. e., S. aureus) of at least 2 after 30 seconds of contact.

Still another aspect of the present inven- tion is to provide an antibacterial composition that exhibits a log reduction against Gram negative bac- teria (i. e., E. coli) of at least 2.5 after 30 seconds of contact.

Another aspect of the present invention is to provide an antibacterial composition that exhib- its a substantial log reduction against Gram posi- tive and Gram negative bacteria, and has a pH of about 5 to about 8.

A present antibacterial composition is phase stable, and typically has a viscosity of about 0.1 to about 50 centipoise (cp). However, in the presence of an optional thickener, the viscosity can be up to about 10,000. The present compositions also exhibit excellent esthetic properties, such as foam height and foam stability. The present comp- ositions further impart skin conditioning and im- proved skin feel to cleansed skin. These improved esthetic and skin care properties are unexpected in antibacterial compositions because skin care and esthetic ingredients are difficult to incorporate into antibacterial compositions, and especially difficult'to incorporate without adversely affecting the antibacterial efficacy of the composition.

Another aspect of the present invention is to provide consumer products based on an antibac- terial composition of the present invention, for example, a skin cleanser, a body splash, a surgical scrub, a wound care agent, a hand sanitizer gel, a disinfectant, a mouth wash, a pet shampoo, a hard surface sanitizer, and the like. The present comp- ositions can be applied, then either rinsed off, wiped off, or allowed to remain on the skin.

A further aspect of the present invention is to provide a method of reducing the Gram positive and/or Gram negative bacteria populations on animal tissue, including human tissue, by contacting the

tissue, like the dermis, with a composition of the present invention for a sufficient time, such as about 15 seconds to 5 minutes, to reduce the bac- teria level to a desired level.

The above and other novel aspects and ad- vantages of the present invention are illustrated in the following, nonlimiting detailed description of the preferred embodiments.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1A and 1B are graphs of number of panelists vs. wash session showing the number of panelists that terminated the study; Fig. 2 contains bar graphs showing the change in baseline for a skin redness study using Composition C and HCPHW-E; and Fig. 3. contains bar graphs showing the water loss change for a study using Composition C and HCPHW-E.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Personal care products incorporating an active antibacterial agent have been known for many years. Since the introduction of antibacterial personal care products, many claims have been made that such products provide antibacterial properties.

However, to be most effective, an antibacterial com- position should provide a high log reduction against a broad spectrum of organisms in as short a contact time as possible.

The antibacterial composition also should exhibit excellent esthetic properties and impart skin care properties in order to achieve consumer acceptance. The features of antibacterial efficacy, esthetics, and skin care often are competing, where- in enhancing one feature is detrimental to the other. The present invention is directed to anti- bacterial compositions that unexpectedly exhibit all of these features.

As presently formulated, most commercial liquid antibacterial soap compositions provide a poor to marginal time kill efficacy, i. e., rate of killing bacteria. Table 1 summarizes the kill effi- cacy of commercial products, each of which contains about 0.2% to 0.3%, by weight, triclosan (an anti- bacterial agent).

Table 1 Time Kill Efficacy of Commercial Liquid Hand Soaps (log reduction after 1 minute contact time) Gram Gram Gram Product positive negative negative S. aureus E. Coli K. pneum. Commercial Product A 1.39 0.00 0.04 Commercial Product B 2.20 0.00 0.01 Commercial Product C 1.85 0.00 0.00 Commercial Product D 2.79 0.26-- Present-day products especially lack efficacy against Gram negative bacteria, such as E. coli, which are of particular concern to human health. For example, note that Commercial Product D

of Table 1, referred to as a"moisturizing antimi- crobial"product is ineffective versus E. coli in a time-kill test. The present invention, therefore, is directed to antibacterial compositions having an exceptionally high broad spectrum antibacterial efficacy, as measured by a rapid kill of bacteria (i. e., time kill), which is to be distinguished from persistent kill, and that provides consumer-accept- able esthetic properties.

The present antibacterial compositions provide significantly improved time kill efficacy compared to prior compositions. The basis of this improved time kill is the discovery that the anti- microbial efficacy of an active agent can be corre- lated to the rate at which the agent has access to an active site on the microbe. The driving force that determines the rate of agent transport to the site of action is the difference in chemical poten- tial between the site at which the agent acts and the external aqueous phase. Alternatively stated, the microbicidal activity of an active agent is proportional to its thermodynamic activity in the external phase. Accordingly, thermodynamic activ- ity, as opposed to concentration, is the more important variable with respect to antimicrobial efficacy. Thermodynamic activity is conveniently correlated to the percent saturation of the active antibacterial agent in the continuous aqueous phase of the composition. This feature is discussed fully in U. S. Patent No. 6,107,621, incorporated herein by reference.

The present compositions are antibacterial compositions having an improved effectiveness

against both Gram negative and Gram positive bac- teria, that exhibit a rapid bacteria kill, that exhibit excellent esthetics, and that impart skin conditioning and improved feel to cleansed skin. As illustrated in the following embodiments, an anti- bacterial composition of the present invention com- prises: (a) about 0. 001% to about 10%, by weight, of an antibacterial agent; (b) about 0.1% to about 40%, by weight, of a surfactant; (c) about 1% to about 40%, by weight, of a hydrotrope; (d) about 1% to about 25%, by weight, of a hydric solvent; (e) 0% to about 5%, by weight, of a skin care agent; (f) 0% to about 2%, by weight, of a foam stabilizer; (g) 0% to about 5%, by weight, of a humectant; and (h) water, wherein the composition contains at least one of (e), (f), and (g).

The compositions have a percent saturation of antibacterial agent in the continuous aqueous phase of at least about 25%, when measured at room temperature. The compositions exhibit a log reduc- tion against Gram positive bacteria of at least about 2 after 30 seconds contact. The compositions exhibit a log reduction against Gram negative bac- teria of at least about 2.5 after 30 seconds con- tact. The compositions also exhibit excellent comp- osition esthetics, e. g., foam characteristics, and viscosity. The compositions further impart skin conditioning properties and improved feel to cleansed skin.

Embodiments of the present invention com- prise (a) an active antibacterial agent, (b) a sur- factant, (c) a hydrotrope, (d) a hydric solvent, (e) at least one of a skin care agent, a foam stabil-

izer, and a humectant, and (f) water. The presence of a hydric solvent, hydrotrope, skin care agent, foam stabilizer, and humectant do not adversely affect the antimicrobial properties of the composi- tion. The compositions are phase stable, and exhib- it excellent esthetic properties, such as foam gen- eration and stability, and impart excellent skin conditioning properties and skin feel. The composi- tions can further include additional optional ingre- dients disclosed hereafter, such as thickeners, preservatives, pH adjusters, dyes, and perfumes.

In particular, the present invention is directed to antibacterial compositions, especially for use in personal care, but also suitable as dis- infectants, surgical scrubs, hospital hand wash products, hand sanitizer gels, wound care agents, and the like. The present compositions comprise about 0.001% to about 10% of a phenolic antibacter- ial agent, preferably triclosan or PCMX, dissolved in an aqueous vehicle and further containing a sur- factant, solvent, hydrotrope, and at least one of a skin care agent, foam stabilizer, and humectant.

The surfactant, solvents, and hydrotropes are pres- ent in amounts such that the percent saturation of the phenolic antibacterial agent in the composition is at least 25%, preferably greater than about 50%, and most preferably greater than about 95% (see U. S.

Patent No. 6,107,261). The foam stabilizing, skin care, and humectant additives are selected from com- pounds including, but not limited to, polymers, pro- tein derivatives, silicone derivatives, ethoxylated derivatives, long-chain fatty materials, and lipid- like materials. The present compositions exhibit

new and unexpected properties, like mildness, skin after-feel, foaming properties, and other properties required, or at least desired, by consumers.

As demonstrated in more detail hereafter, a preferred embodiment contains, by weight, about 0.3% to about 1.0% triclosan, about 5% to about 15% dipropylene glycol, about 10% to about 40% sodium xylene sulfonate, about 0.5% to about 5% ammonium lauryl sulfate, 0% to about 5% cocamidopropyl be- taine, and one or more of 0% to about 3% sodium PCA, 0% to about 0.5% cetyl or cetearyl alcohol, 0% to about 0.5% polyquaternium-10,0% to about 5% glycer- in, and 0% to about 1% aloe.

A. Antibacterial Accent An antibacterial agent is present in a composition of the present invention in an amount of about 0. 001% to about 10%, and preferably about 0. 01% to about 5%, by weight of the composition. To achieve the full advantage of the present invention, the antibacterial agent is present in an amount of about 0.05% to about 2%, by weight, of the composi- tion.

The antibacterial compositions can be ready to use compositions, which typically contain 0.001% to about 2%, preferably 0.01% to about 1.5%, and most preferably about 0.05% to about 1%, of an antibacterial agent, by weight of the composition.

The antibacterial compositions also can be form- ulated as concentrates that are diluted before use with one to about 100 parts water to provide an end use composition. The concentrated compositions

typically contain greater than about 0.1% and up to about 10%, by weight, of the antibacterial agent.

Applications also are envisioned wherein the end use composition contains greater than 2%, by weight, of the antibacterial agent.

As discussed in U. S. Patent No. 6,107,261, the absolute amount of antibacterial agent present in the composition is not as important as the amount of available antibacterial agent in the composition.

The amount of available antibacterial agent in the composition is related to the identity and amount of ingredients in the composition.

To achieve the desired bacteria kill in a short contact time, like 15 to 60 seconds, the com- position contains an amount of antibacterial agent that is at least about 25%, and preferably at least about 50%, of the saturation concentration of the antibacterial agent in the composition, when mea- sured at room temperature. To achieve the full ad- vantage of the present invention, the composition is at least 75%, and more preferably about 95% to 100%, saturated with the antibacterial agent. The method of determining percent saturation of antibacterial agent in the composition is disclosed hereafter.

The antimicrobial agents useful in the present invention are phenolic compounds exemplified by the following classes of compounds:

(a) 2-Hydroxydiphenyl compounds wherein Y is chlorine or bromine, Z is SO2H, Nô2, or C1-C4 alkyl, r is 0 to 3, o is 0 to 3, p is 0 or 1, m is 0 or 1, and n is 0 or 1.

In preferred embodiments, Y is chlorine or bromine, m is 0, n is 0 or 1, o is 1 or 2, r is 1 or 2, and p is 0.

In especially preferred embodiments, Y is chlorine, m is 0, n is 0, o is 1, r is 2, and p is 0.

A particularly useful 2-hydroxydiphenyl compound has the structure: having the adopted name, triclosan, and available commercially under the tradename IRGASAN DP300, from Ciba Specialty Chemicals Corp., Greensboro, NC.

Another useful 2-hydroxydiphenyl compound is 2,2'- dihydroxy-5,5'-dibromo-diphenyl ether. Additional

bisphenolic compounds are disclosed in U. S. Patent No. 6,113,933, incorporated herein by reference.

(b) Phenol derivatives wherein Ri is hydro, hydroxy, Cl-C4 alkyl, chloro, nitro, phenyl, or benzyl; R2 is hydro, hydroxy, C1-C6 alkyl, or halo; R3 is hydro, Cl-C6 alkyl, hydroxy, chloro, nitro, or a sulfur in the form of an alkali metal salt or ammonium salt; R4 is hydro or methyl, and R. is hydro or nitro. Halo is bromo or, prefer- ably, chloro.

Specific examples of phenol derivatives include, but are not limited to, chlorophenols (o-, m-, p-), 2,4-dichlorophenol, p-nitrophenol, picric acid, xylenol, p-chloro-m-xylenol, cresols (o-, m-, p-), p-chloro-m-cresol, pyrocatechol, resorcinol, 4- n-hexylresorcinol, pyrogallol, phloroglucin, carvacrol, thymol, p-chlorothymol, o-phenylphenol, o-benzylphenol, p-chloro-o-benzylphenol, phenol, 4- ethylphenol, and 4-phenolsulfonic acid. Other phenol derivatives are listed in WO 98/55096 and U. S. Patent No. 6,113,933, incorporated herein by reference.

(c) Diphenyl Compounds wherein X is sulfur or a methylene group, R1 and R' are hydroxy, and R2, R'2, R3, R'3, R4, R'4, R5, and Ru-,, independent of one another, are hydro or halo.

Specific, nonlimiting examples of diphenyl compounds are hexachlorophene, tetrachlorophene, dichloro- phene, 2,3-dihydroxy-5,5'-dichlorodiphenyl sulfide, 2,2'-dihydroxy-3,3', 5,5'-tetrachlorodiphenyl sul- fide, 2,2'-dihydroxy-3,5', 5,5', 6,6'-hexachlorodi- phenyl sulfide, and 3,3'-dibromo-5,5'-dichloro-2,2'- dihydroxydiphenylamine. Other diphenyl compounds are listed in WO 98/55096, incorporated herein by reference.

B. Surfactant In addition to the antibacterial agent, a present antimicrobial composition also contains a surfactant. The surfactant is present in an amount of about 0.1% to about 40%, and preferably about 0.3% to about 20%, by weight, of the composition.

To achieve the full advantage of the present inven- tion, the antibacterial composition contains about 0.5% to about 15%, by weight, of the surfactant.

Ready-to-use compositions typically con- tain about 0.1% to about 10%, preferably about 0.3% to about 5%, and most preferably, 0.5% to about 3%, by weight, of the composition. Concentrated com- positions suitable for dilution typically contain greater than about 5%, by weight, of a surfactant.

The amount of surfactant present in the composition is related to the amount and identity of the antibacterial agent in the composition and to the identity of the surfactant. The amount of sur- factant is determined such that the percent satura- tion of the antibacterial agent in the composition is at least about 50%, preferably at least about 75%, and most preferably at least about 95% up to 100%.

The surfactant can be an anionic surfac- tant, a cationic surfactant, a nonionic surfactant, or a compatible mixture of surfactants. The sur- factant also can be an ampholytic or amphoteric sur- factant, which have anionic or cationic properties depending upon the pH of the composition.

The antibacterial compositions, therefore, can contain any anionic surfactant having a hydro- phobic moiety, such as a carbon chain including about 8 to about 30 carbon atoms, and particularly about 12 to about 20 carbon atoms, and further has a hydrophilic moiety, such as sulfate, sulfonate, carbonate, phosphate, or carboxylate. Often, the hydrophobic carbon chain is etherified, such as with ethylene oxide or propylene oxide, to impart a par- ticular physical property, such as increased water solubility or reduced surface tension to the anionic surfactant.

Therefore, suitable anionic surfactants include, but are not limited to, compounds in the classes known as alkyl sulfates, alkyl ether sul- fates, alkyl ether sulfonates, sulfate esters of an alkylphenoxy polyoxyethylene ethanol, alpha-olefin sulfonates, beta-alkoxy alkane sulfonates, alkylaryl sulfonates, alkyl monoglyceride sulfates, alkyl monoglyceride sulfonates, alkyl carbonates, alkyl ether carboxylates, fatty acids, sulfosuccinates, sarcosinates, octoxynol or nonoxynol phosphates, taurates, fatty taurides, fatty acid amide polyoxy- ethylene sulfates, isethionates, or mixtures thereof. Additional anionic surfactants are listed in McCutcheon's Emulsifiers and Detergents, 1993 Annuals, (hereafter McCutcheon's), McCutcheon Division, MC Publishing Co., Glen Rock, NJ, pp. 263- 266, incorporated herein by reference. Numerous other anionic surfactants, and classes of anionic surfactants, are disclosed in Laughlin et al. U. S.

Patent No. 3,929,678, incorporated herein by refer- ence.

Examples of anionic surfactants include a Cl-ci alkyl sulfate, a C8-C18 fatty acid salt, a Cl-ci alkyl ether sulfate having one or two moles of ethoxylation, a C8-C18 alkamine oxide, a C8-C18 alkyl sarcosinate, a Cg-Cis sulfoacetate, a Cl-ci sulfo- succinate, a C8-C18 alkyl diphenyl oxide disulfonate, a Cl-ci alkyl carboxylate, a Cg-Cig alpha-olefin sulfonate, a methyl ester sulfonate, and mixtures thereof. The Ca-C18 alkyl group contains eight to sixteen carbon atoms, and can be straight chain (e. g., lauryl) or branched (e. g., 2-ethylhexyl).

The cation of the anionic surfactant can be an

alkali metal (preferably sodium or potassium), ammonium, C1-C4 alkylammonium (mono-, di-, tri), or C1-C3 alkanolammonium (mono-, di-, tri-). Lithium and alkaline earth cations (e. g., magnesium) can be used, but antibacterial efficacy is reduced.

Specific surfactants include, but are not limited to, lauryl sulfates, octyl sulfates, 2- ethylhexyl sulfates, lauramine oxide, decyl sul- fates, tridecyl sulfates, cocoates, lauroyl sarcos- inates, lauryl sulfosuccinates, linear C,. o diphenyl oxide disulfonates, lauryl sulfosuccinates, lauryl ether sulfates. (1 and 2 moles ethylene oxide), myristyl sulfates, oleates, stearates, tallates, cocamine oxide, decylamine oxide, myristamine oxide, ricinoleates, cetyl sulfates, and similar surfac- tants.

The antibacterial compositions also can contain nonionic surfactants. Typically, a nonionic surfactant has a hydrophobic base, such as a long chain alkyl group or an alkylated aryl group, and a hydrophilic chain comprising a sufficient number (i. e., 1 to about 30) of ethoxy and/or propoxy moieties. Examples of classes of nonionic surfac- tants include ethoxylated alkylphenols, ethoxylated and propoxylated fatty alcohols, polyethylene glycol ethers of methyl glucose, polyethylene glycol ethers of sorbitol, ethylene oxide-propylene oxide block copolymers, ethoxylated esters of fatty (Cs-Cl8) acids, condensation products of ethylene oxide with long chain amines or amides, and mixtures thereof.

Exemplary nonionic surfactants include, but are not limited to, methyl gluceth-10, PEG-20 methyl glucose distearate, an alkyl polyglucoside

(APG), like decyl polyglucoside or lauryl polyglu- coside, PEG-20 methyl glucose sesquistearate, C, 1-15 pareth-20, ceteth-8, ceteth-12, dodoxynol-12, laureth-15, PEG-20 castor oil, polysorbate 20, steareth-20, polyoxyethylene-10 cetyl ether, poly- oxyethylene-10 stearyl ether, polyoxyethylene-20 cetyl ether, polyoxyethylene-10 oleyl ether, poly- oxyethylene-20 oleyl ether, an ethoxylated nonyl- phenol, ethoxylated octylphenol, ethoxylated dodecylphenol, or ethoxylated fatty (C6-C22) alcohol, including 7 to 20 ethylene oxide moieties, polyoxy- ethylene-20 isohexadecyl ether, polyoxyethylene-23 glycerol laurate, polyoxy-ethylene-20 glyceryl stearate, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, polyoxyethylene-20 sorbitan mono- esters, polyoxyethylene-80 castor oil, polyoxyethyl- ene-15 tridecyl ether, polyoxy-ethylene-6 tridecyl ether, PEG 600 dioleate, PEG 400 dioleate, and mixtures thereof.

Numerous other nonionic surfactants are disclosed in McCutcheon's Detergents and Emulsi- fiers, 1993 Annuals, published by McCutcheon Divi- sion, MC Publishing Co., Glen Rock, NJ, pp. 1-246 and 266-272; in the CTFA International Cosmetic Ingredient Dictionary, Fourth Ed., Cosmetic, Toi- letry and Fragrance Association, Washington, D. C.

(1991) (hereinafter the CTFA Dictionary) at pages 1- 651; and in the CTFA Handbook, at pages 86-94, each incorporated herein by reference.

In addition to anionic and nonionic sur- factants, cationic, ampholytic, and amphoteric sur- factants can be used in the antimicrobial composi- tions.

Ampholytic surfactants can be broadly described as derivatives of secondary and tertiary amines having aliphatic radicals that are straight chain or branched, and wherein one of the aliphatic substituents contains from about 8 to 18 carbon atoms and at least one of the aliphatic substituents contains an anionic water-solubilizing group, e. g., carboxy, sulfonate, or sulfate. Examples of com- pounds falling within this description are sodium 3- (dodecylamino) propionate, sodium 3- (dodecylamino)- propane-1-sulfonate, sodium 2- (dodecylamino) ethyl sulfate, sodium 2- (dimethylamino) octadecanoate, di- sodium 3- (N-carboxymethyl-dodecylamino) propane-l- sulfonate, disodium octadecyliminodiacetate, sodium 1-carboxymethyl-2-undecylimidazole, and sodium N, N- bis(2-hydroxyethyl)-2-sulfato-3-dodecoxypropylamine.

More particularly, one class of ampholytic surfactants include sarcosinates and taurates having the general structural formula wherein R1 is Cll through C21 alkyl, R2 is hydrogen or C1-C2 alkyl, Y is CO2M or S03M, M is an alkali metal, and n is a number 1 through 3.

Another class of ampholytic surfactants is the amide sulfosuccinates having the structural formula

The following classes of ampholytic sur- factants also can be used: 0 CH2CO2-Na+ R1CNHCH2CH2t CH2CH20H alkoamphoglycinates 0 fH2c02~Na 11 1 R11CNHCH2CH2NCH2CO2H CH2CH20H alkoamphocarboxyglycinates 0 CH2CH2CO2-Na+ R11CNHCH2CH2N CH2CH20H alkoamphopropionates

0CH2CH2CO2-Na+ 11 1 R1CNHCHCH2NCH2C02H CH2CH20H alkoamphocarboxypropionates OH CH2CHCH2S03-Na+ R1CNHCH 2CH2N CH2CH20H alkoamphopropylsulfonates 0 CH3 R1CNH (CH2) 3N+-CH2C02 CH3 alkamidopropyl betaines O CH3 OH R'CN'H (CH2) 3N+-cH2cHcH CH3 alkamidopropyl hydroxysultaine

0 o RlNHCH2CH2C-O~Na+ alkylaminopropionates CH2CH2COa- I RNH CH2CH2CO2H alkyliminopropionates.

Additional classes of ampholytic surfactants include the phosphobetaines and the phosphitaines.

Specific, nonlimiting examples of ampholytic surfactants useful in the present inven- tion are sodium coconut N-methyl taurate, sodium oleyl N-methyl taurate, sodium tall oil acid N- methyl taurate, sodium palmitoyl N-methyl taurate, cocodimethylcarboxymethylbetaine, lauryldimethyl- carboxymethylbetaine, lauryldimethylcarboxyethyl- betaine, cetyldimethylcarboxymethylbetaine, lauryl- bis- (2-hydroxyethyl) carboxymethylbetaine, oleyldi- methylgammacarboxypropylbetaine, lauryl-bis- (2-hy- droxypropyl)-carboxyethylbetaine, cocoamidodimethyl- propylsultaine, stearylamidodimethylpropylsultaine, laurylamido-bis- (2-hydroxyethyl) propylsultaine, disodium oleamide PEG-2 sulfosuccinate, TEA oleamido PEG-2 sulfosuccinate, disodium oleamide MEA sulfo- succinate, disodium oleamide MIPA sulfosuccinate,

disodium ricinoleamide MEA sulfosuccinate, disodium undecylenamide MEA sulfosuccinate, disodium wheat germamido MEA sulfosuccinate, disodium wheat germ- amido PEG-2 sulfosuccinate, disodium isostearamideo MEA sulfosuccinate, cocoamphoglycinate, cocoampho- carboxyglycinate, lauroamphoglycinate, lauroampho- carboxyglycinate, capryloamphocarboxyglycinate, cocoamphopropionate, cocoamphocarboxypropionate, lauroamphocarboxypropionate, capryloamphocarboxy- propionate, dihydroxyethyl tallow glycinate, cocamido disodium 3-hydroxypropyl phosphobetaine, lauric myristic amido disodium 3-hydroxypropyl phos- phobetaine, lauric myristic amido glyceryl phospho- betaine, lauric myristic amido carboxy disodium 3- hydroxypropyl phosphobetaine, cocoamido propyl mono- sodium phosphitaine, lauric myristic amido propyl monosodium phosphitaine, and mixtures thereof.

The surfactant also can be a cationic alkamine oxide surfactant. An alkamine oxide useful in the present invention contains at least one long hydrocarbon chain containing at least eight carbon atoms. One class of amine oxides is the alkyl di- (lower alkyl) amine oxides, wherein the alkyl group contains 8 to 22, and preferably about 10 to about 16, carbon atoms, and can be straight or branched chain, saturated or unsaturated. The lower alkyl groups contain 1 to 7 carbon atoms, and typically are methyl. Specific examples include, but are not limited to, lauryl dimethyl amine oxide, myristyl dimethyl amine oxide, dimethyl cocoamine oxide, dimethyl (hydrogenated tallow) amine oxide, myrist- yl/palmityl dimethyl amine oxide, myristyl/lauryl dimethyl amine oxide, cetyl dimethyl amine oxide,

stearyl dimethyl amine oxide, and myristyl/cetyl dimethyl amine oxide. These alkamine oxides have a general structural formula Another class of useful amine oxides in- cludes alkyl di (hydroxy lower alkyl) amine oxides in which the alkyl group contains 8 to 22, and prefer- ably about 10 to about 16 carbon atoms, and can be straight or branched chain, saturated or unsatur- ated. Specific examples, include, but are not limited to, bis (2-hydroxyethyl) cocoamine oxide, bis- (2-hydroxyethyl) tallow amine oxide, and bis (2-hy- droxyethyl) stearylamine oxide. These alkamine oxides have a general structural formula Additional useful amine oxides are termed alkamidopropyl di (lower alkyl) amine oxides in which the alkyl group contains 8 to 22, and preferably about 10 to about 16 carbon atoms, and can be

straight or branched chain, saturated or unsatur- ated. Examples are cocoamidopropyl dimethyl amine oxide and tallowamidopropyl dimethyl amine oxide.

These alkamine oxides have a general structural formula Further useful amine oxides are termed alkylmorpholine oxides in which the alkyl group con- tains 8 to 22, and preferably about 10 to about 16, carbon atoms, and can be straight or branched chain, saturated or unsaturated. Alkamine oxides are commercially available, for example, from Stepan Co., Northfield, IL, and Lonza Inc., Fairlawn, NJ.

The above classes of alkamine oxide sur- factants contain a C8-C22alkyl group selected from, for example, octyl, decyl, undecyl, lauryl, tri- decyl, myristyl, cetyl, stearyl, isostearyl, oleyl, and mixtures thereof. Examples of amine oxide sur- factants include, but are not limited to, decyl dimethylamine oxide, lauryl dimethylamine oxide, stearyl dimethylamine oxide, oleyl dimethylamine oxide, coco dihydroxyethylamine oxide, cetyl N, N- dihydroxyethylamine oxide, oleyl N, N-dihydroxyethyl- amine oxide, cocamine oxide, cocamidopropylamine oxide, lauramidopropylamine oxide, oleamine oxide,

oleamidopropylamine oxide, wheat germamidopropyl- amine oxide, isostearamidopropylamine oxide, stear- amine oxide, stearamidopropylamine oxide, cocomorph- oline oxide, decylamine oxide, dihydroxyethyl Cg-Clo- alkoxypropylamine oxide, dihydroxyethyl Cg-Cllalkoxy- propylamine oxide, dihydroxyethyl C12-Cl5alkoxypropyl- amine oxide, dihydroxyethyl cocamine oxide; dihy- droxyethyl stearamine oxide, dihydroxyethyl tallow- amine oxide, hydrogenated tallow amine oxide, hy- droxyethyl hydroxypropylC12-Clsalkoxypropylamine oxide, isostearamidopropyl morpholine oxide, myrist- amidopropylamine oxide, myristamine oxide, palmit- amidopropylamine oxide, palmitamine oxide, PEG-3 lauramine oxide, tallow amidopropylamine oxide, tallow amine oxide, undecylenamidopropylamine oxide, and mixtures thereof. Preferred alkamine oxide sur- factants are the alkyl di (lower alkyl) amine oxides in which the alkyl group contains about 12 to about 16 carbon atoms, including lauramine oxide, myrist- amine oxide, cocamine oxide, cetamine oxide, and mixtures thereof. Most preferably, the alkamine oxide surfactant comprises lauramine oxide.

Additional cationic surfactants include a quaternary surfactant having a structural formula

a quaternized phosphate ester, such as PHOSPHOLIPID SV, available from Mona Industries, Paterson, NJ, e. g., stearamidopropyl phosphatidyl PG-dimonium chloride, linoleamidopropyl phosphatidyl PG-dimonium chloride, coco phosphatidyl PG-dimonium chloride, cocamidopropyl phosphatidyl PG-dimonium chloride, borageamidopropyl phosphatidyl PG-dimonium chloride, and cocohydroxyethyl phosphatidyl PG-imidazolinium chloride; and other quaternized phosphate esters disclosed in Mayhew et al. U. S. Patent No.

4,209,449. Additional quaternary ammonium surfac- tants can be found in the CTFA Handbook at pages 40- 42, incorporated herein by reference.

C. Hydric Solvent and Hydrotrope The present invention also contains about 1% to about 25%, by weight, of a hydric solvent, and 1% to about 40%, by weight, of a hydrotrope.

Preferred embodiments contain about 2% to about 20%, by weight, of a hydric solvent and about 2% to about 25%, by weight, of a hydrotrope. Most preferred embodiments contain about 5% to about 15%, by weight, of a hydric solvent and about 5% to about 20%, by weight, of a hydrotrope.

As defined herein, the term"hydric sol- vent"is a water-soluble organic compound containing one to six, and typically one to three, hydroxyl groups. The term"hydric solvent,"therefore, en- compasses water-soluble alcohols and diols. Spe- cific examples of hydric solvents include, but are not limited to, methanol, ethanol, isopropyl alco- hol, n-butanol, n-propyl alcohol, ethylene glycol,

propylene glycol, diethylene glycol, dipropylene glycol, tripropylene glycol, hexylene glycol, butyl- ene glycol, PEG-4, and similar hydroxyl-containing compounds.

A hydrotrope is a compound that has the ability to enhance the water solubility of other compounds. A hydrotrope utilized in the present invention lacks surfactant properties, and typically is a short-chain alkyl aryl sulfonate. Specific examples of hydrotropes includes, but are not limited to, sodium cumene sulfonate, ammonium cumene sulfonate, ammonium xylene sulfonate, potassium toluene sulfonate, sodium toluene sulfonate, sodium xylene sulfonate, toluene sulfonic acid, and xylene sulfonic acid. Other useful hydrotropes include sodium polynaphthalene sulfonate, sodium polystyrene sulfonate, sodium methyl naphthalene sulfonate, and disodium succinate.

D. Skin Care Axent An antibacterial composition of the pres- ent invention also can contain 0% to about 5%, and preferably 0.1% to about 3%, by weight, of a skin care agent. To achieve the full advantage of the present invention, the composition contains about 0.2% to about 2.5%, by weight, of a skin care agent.

The identity of the skin care agent is not particularly limited, as long as the agent does not adversely affect the stability or efficacy of the composition. One important class of skin care agents is emollients. Emollients are cosmetic in- gredients that help to maintain a soft, smooth, and

pliable skin appearance. Emollients function by remaining on the skin surface or in the stratum corneum to act as lubricants, to reduce flaking, and to improve skin appearance.

In general, the skin care agent includes polymers (e. g., polyvinylpyrrolidine), protein derivatives (e. g., derivatized hydrolyzed wheat protein), ethoxylated fatty ethers, cellulosics (e. g., hydroxyethylcellulose), and similar skin care agents. For example, suitable skin care agents in- clude, but are not limited to, esters comprising an aliphatic alcohol having 2 to about 18 carbon atoms condensed with an aliphatic or aromatic carboxylic acid including 8 to about 20 carbon atoms, e. g., isopropyl myristate, decyl oleate, and cetearyl isononanate. The ester is either straight chained or branched. Preferably, the ester has a molecular weight of less than about 500 and provides emollient properties.

Nonlimiting examples of other skin care agents include, but are not limited to, polyvinyl- pyrrolidone, polyquaternium-4, polyquaternium-7, polyquaternium-10, guar gum derivatives, hydroxy- propylmethylcellulose, hydroxyethylcellulose, a polyethylene glycol, a methyl ether of a polyethyl- ene glycol, quaternium-79, wheat germamidopropyl hydroxypropyl dimonium hydrolyzed wheat protein, stearyl methicone, dimethicone copolyol, dimethicone propyl PG betaine, poly (sodium styrene sulfonate), sorbitan oleate, steareth-2, steareth-21, isoceteth- 20, PEG-7 glyceryl cocoate, PEG-75 lanolin, glycer- eth-26, PPG-5-ceteth-20, a Cl2-C20 alcohol, canola oil, glyceryl laurate, triglyceryl monostearate, glyceryl

monostearate, vitamin E acetate, sunflower seed amidopropylethyldimonium ethylsulfate, sodium PEG-7 olive oil carboxylate, PPG-1 hydroxyethyl capryl- amide, PPG-2 hydroxyethyl cocamide, mineral oil, petrolatum, aloe barbadensis, isostearamidopropyl- morpholine lactate, strontium acetate, and palmit- amidopropyltrimonium chloride. Additional skin care agents are listed in Appendix A. The above skin care agents can be used alone or in admixture.

E. Foam Stabilizer An antibacterial composition of the pres- ent invention also can contain 0% to about 2%, and preferably about 0.05% to about 1.5%, by weight, of a foam stabilizer. To achieve the full advantage of the present invention, the composition contains about 0. 1% to about 1%, by weight, of the foam stabilizer.

The identity of the foam stabilizer is not particularly limited, as long as the stabilizer does not adversely affect the stability and efficacy of the composition. Preferred foam stabilizers are C1o -C22 fatty alcohols (e. g., cetyl alcohol) and C10-C22 fatty acids (e. g., stearic acid). Nonlimiting examples of foam stabilizers include, but are not limited to, behenyl alcohol, Cg. n alcohols, C12-, 3 alcohols, Cl2ls alcohols, C12-,, alcohols, C,,-,, alco- hols, capyrlic alcohol, arachidic acid, arachidonic acid, coconut acid, corn acid, cottonseed acid, hydrogenated coconut acid, hydrogenated menhaden acid, hydrogenated tallow acid, hydroxystearic acid, isostearic acid, cetearyl alcohol, cetyl alcohol,

coconut alcohol, decyl alcohol, isocetyl alcohol, isostearyl alcohol, lauryl alcohol, behenic acid, capric acid, lauric acid, linoleic acid, linolenic acid, linseed acid, myristic acid, oleic acid, palmitic acid, pelargonic acid, octyldodecanol, undecylenyl alcohol, undecylpentadecanol, myristyl alcohol, oleyl alcohol, palm kernel alcohol, stearyl alcohol, tallow alcohol, tridecyl alcohol, caproic acid, caprylic acid, ricinoleic acid, soy acid, stearic acid, tall oil acid, tallow acid, undecanoic acid, undecylenic acid, and mixtures thereof.

F. Humectant An antibacterial composition of the pres- ent invention also can contain 0% to about 2%, and preferably about 0.1% to about 3%, by weight, of a humectant. To achieve the full advantage of the present invention, the composition contains about 0.15% to about 2%, of a humectant.

The identity of the humectant is not par- ticularly limited as long as the humectant does not adversely affect the stability and efficacy of the composition. A humectant typically is a water-sol- uble compound of low volatility, and containing a plurality (i. e., two or more) hydroxyl groups.

Nonlimiting examples of humectants, include, but are not limited to, ascorbic acid, ascorbyl dipalmitate, acetamide MEA, glucose glutamate, glucuronic acid, TEA-lactate, TEA-PCA, corn syrup, fructose, glucose, glycerin, glycol, 1,2,6-hexanetriol, sodium lactate, sodium PCA, hydrogenated starch hydrolysate, inosi- tol, lactic acid, lactose, mannitol, PCA, PEG-10

propylene glycol, polyamino sugar condensate, propylene glycol, pyridoxine dilaurate, saccharide hydrolysate, hydroxystearyl methylglucamine, glucamine, maltitol, mannitol, methyl gluceth-10, methyl gluceth-20, riboflavin, PEG-4, PEG-6, PEG-8, PEG-9, PEG-10, PEG-12, PEG-14, PEG-16, PEG-18, PEG-20, PEG-32, PEG-40, glutamic acid, glycereth-7, glycereth-12, glycereth-26, saccharide isomerate, sorbeth-20, sorbitol, sucrose, thioglycerin, tris- (hydroxymethyl) nitromethane, tromethamine, histi- dine, PEG-75, PEG-135, PEG-150, PEG-200, PEG-5 pentaerythritol ether, polyglyceryl sorbitol, sorbitol, urea, xylitol, and mixtures thereof.

G. Carrier The carrier of the composition comprises water.

H. Optional Ingredients An antibacterial composition of the pres- ent invention also can contain optional ingredients well known to persons skilled in the art, such as dyes and fragrances, that are present in a suffi- cient amount to perform their intended function and do not adversely affect the antibacterial efficacy of the composition. Such optional ingredients typically are present, individually, from 0% to about 5%, by weight, of the composition, and, collectively, from 0% to about 20%, by weight, of the composition.

Classes of optional ingredients include, but are not limited to, dyes, fragrances, pH ad- justers, preservatives, thickeners, viscosity modi- fiers, buffering agents, antioxidants, foam en- hancers, chelating agents, opacifiers, and similar classes of optional ingredients known to persons skilled in the art.

Specific classes of optional ingredients include alkanolamides as foam boosters; parabens as preservatives; inorganic phosphates, sulfates, and carbonates as buffering agents; EDTA and phosphates as chelating agents; and acids and bases as pH ad- justers.

Examples of preferred classes of basic pH adjusters are ammonia; mono-, di-, and tri-alkyl amines; mono-, di-, and tri-alkanolamines; alkali metal and alkaline earth metal hydroxides; and mixtures thereof. However, the identity of the basic pH adjuster is not limited, and any basic pH adjuster known in the art can be used. Specific, nonlimiting examples of basic pH adjusters are ammonia; sodium, potassium, and lithium hydroxide; monoethanolamine; triethylamine; isopropanolamine; diethanolamine; and triethanolamine.

Examples of preferred classes of acidic pH adjusters are the mineral acids and polycarboxylic acids. Nonlimiting examples of mineral acids are hydrochloric acid, nitric acid, phosphoric acid, and sulfuric acid. Nonlimiting examples of polycarbox- ylic acids are citric acid, glycolic acid, and lactic acid. The identity of the acidic pH adjuster is not limited and any acidic pH adjuster known in the art, alone or in combination, can be used.

An alkanolamide to provide foam enhance- ment can be, but is not limited to, cocamide MEA, cocamide DEA, soyamide DEA, lauramide DEA, oleamide MIPA, stearamide MEA, myristamide MEA, lauramide MEA, capramide DEA, ricinoleamide DEA, myristamide DEA, stearamide DEA, oleylamide DEA, tallowamide DEA, lauramide MIPA, tallowamide MEA, isostearamide DEA, isostearamide MEA, and mixtures thereof.

A present antibacterial composition also can contain a preservative in an amount of 0% to about 0.5% by weight. Examples of preservatives in- clude, but are not limited to, sorbic acid, potassi- um sorbate, the parabens (like benzylparaben), imid- azolinylurea, methylchloroisothiazolinone, and the hydantoins, like DMDM hydantoin. Additional pre- servatives as disclosed in the CTFA Handbook at page 78, incorporated herein by reference.

A present antibacterial composition fur- ther can contain an antioxidant and/or an ultra- violet light (W) absorber, each independently in an amount of 0% to about 0.5% by weight. Examples of antioxidants of UV absorbers include, but are not limited to, BHA, BHT, sodium ascorbate, potassium sulfite, erythorbic acid, benzophenone-1 through benzophenone-12, and PABA. Additional antioxidants and W absorbers can be found in the CTFA Handbook at pages 78 and 98, incorporated herein by refer- ence.

In addition, the antibacterial composi- tions of the present invention do not rely upon a low pH or a high pH to provide a rapid reduction in bacterial populations. Antibacterial compositions

of the present invention can have a pH of about 4 to about 9, but at the two extremes of this pH range, the compositions can be irritating to the skin or damaging to other surfaces contacted by the composi- tion. Accordingly, antibacterial compositions of the present invention preferably have a pH of about 5 to about 8, and more preferably about 6 to about 8. To achieve the full advantage of the present invention, the antibacterial compositions have a pH of about 6.5 to about 7.5.

To demonstrate the new and unexpected re-. sults provided by the antibacterial compositions of the present invention, the examples in Appendix A were prepared, and the ability of the compositions to control Gram positive and Gram negative bacteria was determined. The weight percentage listed in each of the examples represents the actual, or active, weight amount of each ingredient present in the composition. The compositions were prepared by blending the ingredients, as understood by those skilled in the art.

The following materials were used as in- gredients in the examples. The source of each in- gredient and its abbreviation are summarized below:

Abbrevi- Chemical Name Trade Name Supplier ation Surfactants Ammonium Lauryl STANDAPOL A Cognis ALS Sulfate (28.3% active) Corporation Ambler, PA Sodium Lauryl STANDAPOL ES-2 Cognis SLES2 Ether Sulfate (25.71% active) Corporation (2-mole) Ammonium Cocyl JORDAPON ACI-BASF ACI Isethionate 30G Isethionate Corporation (25% active) Mount Olive, NJ Cocamidopropyl-MACKAM 35-HP McIntyre Group CAPB betaine (about 30% Chicago, IL active) Hydrotropes Sodium Xylene STEPANATE SXS Stepan Company SXS Sulfonate (40-42% active) Northfield, IL Hydric Solvents Dipropylene Dipropylene Ashland DPG Glycol Glycol Chemical Co. (100% active) Covington, KY Polymers Polyvinylpyrrol-PVP K-15 International PVPK15 idone (98-99% active) Specialty Products Wayne, NJ Polyvinylpyrrol-PVP K-30 International PVPK30 idone (98-99% active) Specialty Products Guar Gum, 2-JAGUAR C13S Rhodia JAGC13S Hydroxy-3- (Tri- (88-94% active) Cranbury, NJ methylammonio)- Propyl Ether Chloride Guar Gum, 2-Hy-JAGUAR C14S Rhodia JAGC14S droxy-3- (Tri- (88-94% active) methylammonio)- Propyl Ether Chloride Guar Gum, 2-Hy-JAGUAR C162 Rhodia JAGC162 droxy-3- (Tri- (91% active) methylammonio)- Propyl Ether Chloride Guar Gum, 2-Hy-JAGUAR HP8 Rhodia JAGHP8 droxypropyl (88-94% active) Ether Guar Gum, 2-Hy-JAGUAR HP60 Rhodia JAGHP60 droxypropyl (87-94% active) Ether Guar Gum, 2-Hy-JAGUAR HP105 Rhodia JAGHP105 droxypropyl (90-97% active) Ether

Abbrevi- Chemical Name Trade Name Supplier ation Guar Gum, 2-Hy-JAGUAR HP120 Rhodia JAGHP120 droxypropyl (91-95% active) Ether Polyquaternium-7 MERQUAT 550 Calgon MQ550 (9% active) Corporation Pittsburgh, PA Polyquaternium-4 CELQUAT SC-230M National Starch CQSC230M (100% active) & Chemical Bridgewater, NJ Polyquaternium-CELQUAT SC-240C National Starch CQSC240C 10 (100% active) & Chemical Polyquaternium-4 CELQUAT H-100 National Starch CQH100 (100% active) & Chemical Hydroxypropyl-METHOCEL 40-100 Dow Chemical MCL40100 methylcellulose (90-95% active) Co. Midland, MI Hydroxyethyl-NATROSOL 250 Aqualon/NATSOL250 cellulose HHR Hercules HHR (95-100% Wilmington, DE active) PEG-6 & PEG-32 CARBOWAX Sentry Dow Chemical CWAX540 Polyethylene Co. Glycol 540 Midland, MI (100% active) PEG-18 CARBOWAX Sentry Dow Chemical CWAX900 Polyethylene Co. Glycol 900 (100% active) MethoxyPEG-1000 CARBOWAX Dow Chemical MET5000 Methoxypolyeth-Co. ylene glycol 5000 (100% active) MethoxyPEG-40 CARBOWAX Dow Chemical MET2000 Methoxypolyeth-Co. yleneglycol 2000 (100%active)

Abbrevi- Chemical Name Trade Name Supplier ation PEG-100 CARBOWAX Dow Chemical PG4600 Polyethylene-Co. glycol 4600 (100% active) PEG-6ME PEG6ME Dow Chemical PEG6ME (100% active) Co. PEG-45M POLYOX WSR-N-60 Amerchol WSRN60 (100% active) Institute, WV PEG-14M POLYOX WSR-205 Amerchol WSR205 (100% active) PEG-14M POLYOX WSR-N-Amerchol WSR-N- 3000 3000 (99% active) Poly (sodium FLEXAN 130 National Starch FLEX130 styrene (30% active) Chemical sulfonate) Protein Derivatives Wheatgermamido-MACKPRO WWP McIntyre Group WWP propyl Hydroxy- (35% active) propyl Dimonium Hydrolyzed Wheat Protein Quaternium-79 MACKPRO NLW McIntyre Group NLW Hydrolyzed Wheat (33% active) Protein Silicone Derivatives Dimethicone ABIL B 9950 Goldschmidt DIMETHPGB Propyl PG (29-31% active) Hopewell, VA Betaine Stearyl SILCARE 41M30 Clariant STMETH Methicone (88% active) Gainesville, FL Dimethicone Dow Corning 193 Dow Corning DC193 Copolyol (100% active) Auburn, MI Humectants Glycerine Glycerin, USP Cognis/Emery GLY (100% active) Cincinnati, OH Sodium PCA AJIDEW NL-50 Ajinomoto NaPCA (50% active) Teaneck, NJ Steareth-2 Polyoxyethyl-ICI Americas BRIJ72 ene- (2) stearyl Bridgewater, NJ ether (BRIJ 72) (99% active) Steareth-21 Polyoxyethyl-ICI Americas BRIJ721 ene- (21) stear- yl ether (BRIJ 721) (99% active) Isoceteth-20 ARLASOLVE ICI Americas ARL200 200 (73% active)

Abbrevi- Chemical Name Trade Name Supplier ation PEG-7Glyceryl CETIOL HE Cognis PEG7GC Cocoate (100% active) Corporation PEG-75 Lanolin FANCOR LAN AQUA Fanning PEG75LAN 501 Corporation (100% active) Chicago, IL Sorbitan Oleate ARLACEL 80 ICI Americas ARL80 (100% active) Cocoglucoside LAMESOFT PO-65 Cognis LMSFT and Glyceryl (65% active) Corporation Oleate Glycereth-26 JEECHEM GL-26 Jeen Inter- (100% active) national Corp. Little Falls, NJ PPG-5-Ceteth-20 PROCETYL AWS Croda PPG5CET20 (100% active) Parsippany, NJ Long-chain Fatty Materials Cetyl alcohol Cetyl alcohol Aldrich CETOH (100% active) Milwaukee, WI Cetearyl alcohol STENOL 1618 Cognis CETEAROH (100% active) Corporation Stearic Acid Stearic Acid Aldrich StAC (100% active) Isopropyl KESSCO IPM Stepan Company IPM Myristate (100% active) Decyl Oleate CETIOL V Cognis DCYLOL (100% active) Corporation Cetearyl CETIOL SN Cognis CETISONON Isononanate (100% active) Corporation Lipid-like Materials Canola Oil Canola Oil Procter & CANOL (100% active) Gamble Cincinnati, OH Glyceryl Laurate LAURICIDIN Med-Chem Labs, LRCDN (100% active) Inc. Galena, IL Triglyceryl TGMS Monostearate Glyceryl EMEREST 2400 Cognis GMS Monostearate (100% active) Corporation Other Materials Mackalene 1216 MACKERNIUM 1216 McIntyre Group MAC1216 (24%active) Sunflower seed MACKERNIUM SFES McIntyre Group SFES amidopropyleth- (80% active) yldimonium ethylsulfate Sodium PEG-7 OLIVEM 400 B&T OL400 Olive Oil (35% active) Milano, IT Carboxylate Vitamin E Vitamin E Roche VitEAc Acetate Acetate Nutley, NJ (100% active)

Abbrevi- Chemical Name Trade Name Supplier ation PPG-1 PROMIDIUM CC Uniquema PCC Hydroxyethyl (100% active) Paterson, NJ Caprylamide PPG-2 PROMIDIUM CO Uniquema PCO Hydroxyethyl (100% active) Cocamide Mineral Oil Mineral Oil Mallinckradt MO (100% active) Hazelwood, MO Petrolatum PETR Aloe Barbadensis ACTIVERA 104 Active Organics ALOE Leaf Juice active) Lewisville, TX Isostearamido-MACKALENE 426 McIntyre Group ISML propylmorpholine (25% active) Lactate Strontium Sr (OAC) 2 Aldrich Sr (OAC) 2 Acetate (100% active) Palmitamido-VARISOFT PATC Goldschmidt VRSFT propyltrimonium (57-61% active) Chloride Antimicrobial Agent Triclosan IRGASAN DP-300 Ciba Specialty TCS (100% active) Chemicals Corp. Greensboro, NC The following methods were used in the preparation and testing of the examples: a) Determination of Rapid Germicidal (Time Kill) Activity of Antibacterial Products. The activity of antibacterial compositions was measured by the time-kill method, whereby the survival of challenged organisms exposed to an antibacterial test composition is determined as a function of time. In this test, a diluted aliquot of the com- position is brought into contact with a known population of test bacteria for a specified time period at a specified temperature. The test compo- sition is neutralized at the end of the time period, which arrests the antibacterial activity of the com- position. The percent or, alternatively, log reduc-

tion from the original bacterial population is calculated. In general, the time kill method is known to those skilled in the art.

The composition can be tested at any con- centration from 0-100%. The choice of which concen- tration to use is at the discretion of the investi- gator, and suitable concentrations are readily determined by those skilled in the art. For exam- ple, viscous samples usually are tested at 50% dilu- tion, whereas nonviscous samples are not diluted.

The test sample is placed in a sterile 250 mL beaker equipped with a magnetic stirring bar, and the sample volume is brought to 100 mL, if needed, with sterile, deionized water. All testing is performed in triplicate, the results are combined, and the average log reduction is reported.

The choice of contact time period also is at the discretion of the investigator. Any contact time period can be chosen. Typical contact times range from 15 seconds to 5 minutes, with 30 seconds and 1 minute being typical contact times. The con- tact temperature also can be any temperature, typ- ically room temperature, or about 25 degrees Celsius.

The bacterial suspension, or test inoc- ulum, is prepared by growing a bacterial culture on any appropriate solid media (e. g., agar). The bac- terial population then is washed from the agar with sterile physiological saline, and the population of the bacterial suspension is adjusted to about 108 colony forming units per mL (cfu/mL).

The table below lists the test bacterial cultures used in the following tests and includes

the name of the bacteria, the ATCC (American Type Culture Collection) identification number, and the abbreviation of the name of the organism used here- after. Organism Name ATCC # Abbreviation Staphylococcusaureus 6538 Sa Escherichia coli 11229 Ec Serratia marcescens 14756 Sm Klebsiella neumoniae 10031 Kp Staphylococcus aureus is a Gram positive bacteria, whereas Escherichia coli and Serratia marcescens are Gram negative bacteria. Many form- ulations were screened for antibacterial efficacy using Serratia marcescens because Sm is relatively difficult to kill rapidly and is used as a test organism in the"Health Care Personnel Hand wash Test"described in"21 CFR Parts 333 and 369 Tentative Final Monograph for Heath Care Antiseptic Drug Products; Proposed Rule" (Food and Drug Admin- istration, Federal Register, Vol. 59, No. 116, Friday, June 17,1994 Proposed Rules).

The beaker containing the test composition is placed in a water bath (if constant temperature is desired), or placed on a magnetic stirrer (if ambient laboratory temperature is desired). The sample then is inoculated with 1.0 mL of the test bacterial suspension. The inoculum is stirred with the test composition for the predetermined contact time. When the contact time expires, 1.0 mL of the test composition/bacteria mixture is transferred into 9.0 mL of Tryptone-Histidine-Tween Neutralizer

Solution (THT). Decimal dilutions to a countable range then are made. The dilutions can differ for different organisms. Selected dilutions are plated in triplicate on TSA+ plates (TSA+ if Trypitcase Soy Agar with Lecithin and Polysorbate 80). The plates then are incubated for 252 hours, and the colonies are counted for the number of survivors, and the percent or log reduction is calculated. The control count (numbers control) is determined by conducting the procedure as described above with the exception that THT is used in place of the test composition.

The plate counts are converted to cfu/mL for the numbers control and samples, respectively, by standard microbiological methods. The log reduction is calculated using the formula Log reduction=Logl, (numbers control) -log10 (test sample survivors).

The following table correlates percent reduction in bacterial population to log reduction. % Reduction Log Reduction 90 1 99 2 99.9 3 99.99 4 99.999 5

b) Physical Stability Screening. The stability of test compositions was determined by observing the compositions several days after prep- aration to determine whether phase separation oc- curred. This screening test was used to determine whether the test composition would be tested fur- ther. c) Foam Property Screening. The foam properties and end use performance enhancement of the compositions was determined by the following two methods: 1) Bottle Shake Foam Test. This test was performed by inverting bottles containing test compositions and timing the persistence of the foam head. In a typical test, eight to ten composi- tions (each contained in a capped, 1L, French square bottle) are tested as a set. Each set includes a control which has the same base formula as the others, but does not contain any performance-enhanc- ing additives. The set of samples first is allowed to equilibrate at a common temperature (usually about 25°C). The bottles then are arranged in a row and inverted five times each, all within about 1 minute. The bottles then are allowed to stand for about 1 to 3 hours, and the time of foam collapse (as judged by an opening in the foam head equal to about 2.5 cm) is recorded. The foam collapse times are compared to the control and summarized as shown in the table below:

Foam Rating (Bottle or Pump Test) Description +++ Bottle Foam stable for several days ++ Foam persisted longer than the test time + Foam persisted longer than control sample, but less than total test time 0 Foam collapsed at the same time as control Foam collapsed sooner than control Foam collapsed almost immediately NT Not tested 2) Pump Foam Test. Because a pre- ferred route of application is use of a self-foaming pump, this test assesses stability of test sample foam ejected from this type of pump. The self-foam- ing pump used in this test is manufactured by Air- spray International B. V., Alkmaar, Holland (model Airspray 1.65 ml TT Pump with EVA (PIB) liner). This test was performed by ejecting one pump stroke of foam on a precleaned watch glass (100mm, Corning Glass Works, #9985) and observing the time of foam collapse. In a typical test, 8 to 10 samples (each contained in a plastic bottle equipped with a foam- ing pump) are tested as a set. As in the Bottle Foam Test, each set includes a control which has the same base formula as the others, but does not con- tain any performance-enhancing additives. The set of samples first is allowed to equilibrate at a common temperature (usually about 25°C). The pumps/ bottles and corresponding watch glasses are arranged in two parallel rows. The pumps are primed with

three strokes just prior to the test. One pump- stroke of foam is ejected onto the corresponding watch glass of each sample, all within about 1 min- ute. The foam samples then are allowed to stand for about 1 to 3 hours, and the time of foam collapse (as judged by circle of bubbles about 5 mm or less) is recorded., The foam collapse times are compared to the control and summarized as shown in the table above. d) Preparation of Samples. The prepara- tion of all samples involved equipment and proce- dures normally employed in formula development laboratories. All percents were by weight based on the active level of each ingredient. e) Summary formula descriptions in exam- ple tables. A typical table entry for a test compo- sition is"0. 6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/0.2- PVPK15."This entry is defined as 0.6% triclosan (TCS), 5% dipropylene glycol (DPG), 15% sodium xylene sulfonate (SXS), 1.5% ammonium lauryl sulfate (ALS), 0.5% cocamidopropyl betaine (CAPB), 0.2% polyvinylpyrrolidone polymer (PVP K-15), and the re- mainder of the formula is water (typically with 0.2%, by total weight, of a citrate/phosphate buffer designed to provide a pH of about 6). f) Preparation of saturated solutions of TCS in water. A four-liter flask was equipped with a 3-inch magnetic stir bar and charged with approx- imately 7.5 grams (g) TCS and 3 liters (L) of water.

The flask then was placed in a water bath, stirred, and heated (40-45°C) for at least 8 hours. The flask containing the resulting TCS/water suspension was removed from the water bath, and the warm sus-

pension filtered through a Coors #32-H porcelain Buchner funnel equipped with Whatman #40 (5.5 cm) filter paper. The filtering assembly was attached to a two-liter vacuum filter flask, and filtration was conducted in batches. The filtrate then was transferred to another four-liter flask and allowed to cool. Typically, fine needles of TCS crystals formed after the filtrate was stored at room temper- ature for a few days.

For some time-kill studies, the TCS solu- tion was refiltered at room temperature before use in the study. For other time-kill studies, a small amount of crystalline TCS was allowed to remain in the test container to ensure saturation in the event of a temperature change. It was assumed that TCS crystals present in the time-kill test vessel would not affect test results because crystalline TCS is unavailable to act on the bacteria (i. e., is not solubilized).

To determine the concentration of TCS in the water solutions, filtered samples (in tripli- cate) were analyzed by HPLC. The apparatus used to filter the solutions was a Whatman AUTOVIAL@, with 0.45 urn PTFE membrane and glass microfiber pre- filter, cat. No. AV125UORG. TCS concentrations were calculated using a linear regression line fit (Microsoft EXCELS software) to TCS/IPA standards included on the same HPLC run.

The following examples demonstrate that the new and unexpected results achieved by the pres- ent invention are attributed (in part) to a selec- tion of esthetic enhancing and skin care additives

which maintain a phase-stable system, do not hinder antibacterial activity, and contribute to composi- tion performance and esthetics.

EXAMPLE 1A Phase stability and foam performance attributed to polyvinylpyrrolidone (PVP) polymer additives--The compositions in this example demon- strate the phase stability and performance observed during testing of compositions containing PVP poly- mer additives. In this test, PVP K-15 failed to improve foam properties in the base formula evalu- ated, whereas PVP K-30 exhibited foam property im- provement at higher surfactant levels. Stable Bottle Pump (S)/Not Foam Foam Polymers Comment Formula Stable Test Test (NS) PVPK15 MW=8,000 0. 6TCS/5DPG/15SXS/ S-- 1.5ALS/0.5CAPB/ 0.2PVPK15 PVPK30 MW=38, 000 0. 3TC5/5DPG/15SXS/ NS NT NT 0.75ALS/0.05PVPK30 PVPK30 MW=38,000 0.3TCS/5DPG/15SXS/ NS NT NT 0.75ALS/0.02PVPK30 PVPK30 MW=38,000 1.0TCS/5DPG/15SXS/ S ++ ++ 2.5ALS/0.75CAPB/ 0.1PVPK3 EXAMPLE 1B Phase stability and foam performance attributed to modified guar polymer additives--The compositions in this example demonstrate the phase

stability observed during testing of compositions containing modified guar polymer additives. The nonionic 2-hydroxypropyl ether guar gum polymers were successfully incorporated into the composi- tions. However, two moderately charged cationic polymers (JAGUAR C13S and C14S) were not stable in the base formula. JAGUAR C162, a similar polymer having less charge density, was effectively in- corporated into compositions of the present inven- tion. JAGUAR HP-60-containing compositions exhibited excellent slip properties for dry applica- tion, when the polymer is present in a sufficient amount to provide a perceivable esthetic improve- ment, but not an amount such that the composition is too slippery and too thick for use with a self- foaming pump. Stable (S)/ Bottle Form Pump Foam Not Stable Test Test Polymers Comment Formula (NS) JAGUAR HP-8 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S 2-Hydroxypropyl 0.5HP8 + + Ether JAGUAR HP-60 Guar Gum, 1.0TCS/5DPG/15SXS/2.5ALS/ S 0 2-Hydroxypropyl 0.75CAPB/0.2HP60 + Ether JAGUAR HP-60 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S - 2-Hydroxypropyl 0.2HP60 + Ether JAGUAR HP-60 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S 2-Hydroxypropyl 0.1HP60 + + Ether JAGUAR HP-60 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S 2-Hydroxypropyl 0.1HP60 + + Ether JAGUAR HP-60 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S - 2-Hydroxypropyl 0.05HP60 + Ether JAGUAR HP-60 Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S - - 2-Hydroxypropyl 0.075HP60 Ether JAGUAR HP- Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S - 2-Hydroxypropyl 0.5HP105 + Ether Stable (S) / Bottle Foam Pump Foam Not Stable Test Test Polymers Comment Formula (NS) JAGUAR HP- Guar Gum, 0.3TCS/5DPG/15SXS/0.75ALS/ S 120 2-Hydroxypropyl 0.05HP120 + + Ether JAGUAR C13S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.5JAGC13S methylammonio) - propyl ether chloride JAGUAR C13S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.5JAGC13S methylammonio) - propyl ether chloride JAGUAR C13S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.2JAGC13S methylammonio) - propyl ether chloride JAGUAR C14S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.5JAGC14S methylammonio) - propyl ether chloride JAGUAR C14S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.5JAGC14S methylammonio) - propyl ether chloride Stable (S)/ Bottle Foam Pump Foam Not Stable Test Test Polymers Comment Formula (NS) JAGUAR C14S Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ NS NT NT Hydroxy-3- (tri- 0.5JAGC14S methylammonio) - propyl ether chloride JAGUAR C162 Guar Gum, 2- 0.3TCS/5DPG/15SXS/0.75ALS/ S - Hydroxy-3- (tri- 0.05C162 methylammonio) - propyl ether chloride

EXAMPLE 1C Phase stability and foam performance attributed to a cationic copolymer containing 50% dimethyl diallyl ammonium chloride (DMAAC) and 50% acrylamide additive--The compositions in this ex- ample demonstrate the phase stability observed by incorporating a highly charged polymer into the composition. It was found that a relatively high surfactant level was required to successfully incorporate a highly charged polymer into the comp- osition, even at a 0.05% polymer. As described in U. S. Patent No. 6,107,261, the highest antimicrobial activity is obtained for compositions having a high % saturation of antimicrobial agent. Thus, raising the surfactant level to accommodate solubilization of the polymer or other additives, requires a higher level of antibacterial agent in the composition to maintain a high % saturation. For example, in the first composition of this example, 1. 0% TCS was re- quired to maintain the desired % saturation vs. 0.3% in compositions containing a lower amount of surfac- tant. Stable (S)/ Bottle Foam Pump Foam Not Stable Test Test Polymers Comment Formula (NS) MERQUAT 550 MW = 1,600,000/- 1.0TCS/5DPG/15SXS/ highly charged 2.5ALS/0.75CAPB/0.2MQ5 cationic copolymer 50 MERQUAT 550 " " " 0.3TCS/5DPG/15SXS/0.75 NS NT NT ALS/0.05MQ550 MERQUAT 550 " " " 0.3TCS/5DPG/15SXS/0.75 NS NT NT ALS /0.05MQ550 MERQUAT 550 " " " 0.3TCS/5DPG/15SXS/0.75 NS NT NT ALS/0.05MQ550

EXAMPLE 1D Phase stability and foam performance attributed to cationic hydroxyethylcellulose polymer additives--CELQUAT SC-230M and SC-240C each have a hydroxyethylcellulose (HEC) backbone further deriva- tized with 2-hydroxy (trimethylammonio) propyl ether to provide a cationic polymer. The average molec- ular weight of the HEC backbone of SC-240C is about 63% that of SC-230M. The performance of these two polymers is similar except SC-230M produced a higher composition viscosity at a lower weight % level.

Thus, SC-240C is preferred polymer for use with a foaming pump because of a lower viscosity, which is attributed to, but not relied upon, a lower molec- ular weight of this polymer compared to SC-230M.

CELQUAT H-100 has an HEC backbone which is derivatized with polyDMDAC, and has a high localized nitrogen charge density. In the present composi- tions, CELQUAT H-100 provides excellent foam stabil- ity, skin feel, and skin care properties. Stable (S)/ Not Stable Bottle Pump Foam Polymers Comment Formula (NS) Foam Test Test CELQUAT MW = 1,750,000 1.0TCS/5DPG/15SXS/2.5ALS/ S ++ + SC-230M 0.75CAPB/0.1CQSC230M CELQUAT MW = 1,750,000 0.3TCS/5DPG/15SXS/0.75ALS/ S + - SC-230M 0.1CQSC230M CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S + - SC-230M 0.5CAPB/0.3CQSC230M CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S + + SC-230M 0.5CAPB/5GLY/0.5CQSC230M CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S - ++ SC-230M 0.75CAPB/0.5CQSC230M CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S + + SC-230M 0.5CAPB/5GLY/0.5CQSC230M CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S + - SC-240M 0.5CAPB/0.5CQSC240C CELQUAT MW = 1,750,000 0.6TCS/5DPG/15SXS/1.5ALS/ S + + SC-240M 0.5CAPB/1.0CQSC240C CELQUAT MW = 1,400,000 0.6TCS/5DPG/15SXS/1.5ALS/ S ++ ++ H-100 0.75CAPB/0.5CQH100

EXAMPLE 1E Phase stability and foam performance attributed to hydroxypropylcellulose (HPC) and hydroxyethylcellulose (HEC) polymer additives-- Compositions containing the HPC polymer exhibited acceptable foam properties, but marginal phase in- stability, at lower surfactant levels. The composi- tion containing the HEC polymer was phase stable, but foam properties were not improved. Stable (S)/ Not Stable Bottle Pump Foam Polymers Comment Formula (NS) Foam Test Test METHOCEL Hydroxypropyl- 0.3TCS/5DPG/15SXS/ S + + 40-100 cellulose 0.75ALS/0.1MCL40100 (turbid) METHOCEL " " " 0.3TCS/5DPG/15SXS/ NS NT NT 40-100 0.75ALS/0.2MCL40100 METHOCEL " " " 0.3TCS/5DPG/15SXS/ S + + 40-100 0.75ALS/0.05MCL40100 (turbid) METHOCEL " " " 1.0TCS/5DPG/15SXS/ S ++ + 40-100 2.5ALS/0.75CAPB/ 0.2MCL40100 NATROSOL Hydroxyethylcellulose 0.3TCS/5DPG/15SXS/ S - - 250 HHR 0.75ALS/ 0.05NATSOL250HHR

EXAMPLE 1F Phase stability and foam performance attributed to polyethylene glycol (PEG) and methoxy- polyethylene glycol (MPEG) polymer additives--The compositions in this example illustrate the effect of increasing polymer chain length on phase stabil- ity, i. e., longer polymer chains decrease composi- tion stability. In addition, while shorter chain polymers provided a stable base formula, foam per- formance was best for the shortest chain polymer (PEG6ME). Stable (S)/ Not Stable Bottle Pump Foam Polymers Comment Formula (NS) Foam Test Test PEG6ME MW = 335 to 365 0.6TCS/5DPG/15SXS/1.5 S ++ - ALS/0.5CAPB/1PEG6-ME CARBOWAX 540 MW = 468 to 534 0.6TCS/5DPG/15SXS/ S - - (PEG-6 & PEG-32) 1.5ALS/0.5CAPB/1.0CWA X540 CARBOWAX 900 MW = 855 to 945 0.6TCS/5DPG/15SXS/ S - - (PEG-18) 1.5ALS/0.5CAPB/1.0CWA X900 Methoxypolyeth- MW = 1800 to 2200 0.6TCS/5DPG/15SXS/ S - - ylene glycol 2000 1.5ALS/0.5CAPB/0.5MET (Methoxy PEG-40) 2000 Polyethylene glycol MW = 4140 to 5060 0.6TCS/5DPG/15SXS/ S - - 4600 (PEG-100) 1.5ALS/0.5CAPB/0.5PG4 600 Methoxypolyeth- MW = 4375 to 5675 0.6TCS/5DPG/15SXS/ S - - ylene glycol 5000 1.5ALS/0.5CAPB/0.5MET (Methoxy PEG 1000) 5000 POLYOX WSR-N-3000 MW = 400,000 0.6TCS/5DPG/15SXS/ S NT NT 1.5ALS/0.5CAPB/1WSR- (slightly N-3000 turbid) POLYOX WSR-205 MW = 600,000 0.6TCS/5DPG/15SXS/ S NT NT 1.5ALS/0.5CAPB/0.5WSR (turbid) 205 POLYOX WSR-N-60 MW = 2,000,000 0.6TCS/5DPG/15SXS/ NS NT NT 1.5ALS/0.5CAPB/0.05WS RN60

EXAMPLE 1G Phase stability and foam performance attributed to a poly (sodium styrene sulfonate) poly- mer additive--This example illustrates the perform- ance of an anionic polymer additive. This polymer provided a stable composition, but marginal lather performance. Stable (S)/ Bottle Foam Pump Foam Test Not Stable (NS) Test Polymers Comment Formula FLEXAN 130 0.6TCS/5DPG/15SXS/1.5ALS/ S - - 0.5CAPB/1.0FLEX130

EXAMPLE 2 Phase stability and foam performance attributed to protein derivative additives--A major- ity of the compositions evaluated in this example were phase stable and exhibited moderate foam prop- erty enhancement. NLW was solubilized more easily by the base composition than WWP. Stable (S)/ Protein Not Stable Bottle Pump Foam Derivative Comment Formula (NS) Foam Test Test MACKPRO WWP Wheatgermamidopropyl 0.3TCS/5DPG/15SXS/ S - + Hydroxypropyl Dimonium 0.75ALS/0.1WWP Hydrolyzed Wheat Protein MACKPRO WWP " " " 1.0TCS/5DPG/15SXS/ NS NT NT 2.5ALS/0.75CAPB/0.2WWP MACKPRO WWP " " " 0.3TCS/5DPG/15SXS/ S + - 0.75ALS/1.0WWP MACKPRO WWP " " " 0.3TCS/TDPG/15SXS/ S + + 0.75ALS/0.5WWP MACKPRO NLW Quaternium-79 Hydrolyzed 0.3TCS/5DPG/15SXS/ S + - Wheat Protein 0.75ALS/0.2NLW MACKPRO NLW " " " 1.0TCS/5DPG/15SXS/ S 0 + 2.5ALS/0.75CAPB/0.1NLW MACKPRO NLW " " " 1.0TCS/5DPG/15SXS/ S + + 2.5ALS/0.75CAPB/0.2NLW

EXAMPLE 3 Phase stability and foam performance attributed to humectant additives--Two humectants, glycerin and sodium pyrrolidone carboxylate (sodium PCA), were evaluated. This example shows that phase stability is not adversely affected by these humec- tants, and that the amount of humectant can be ad- justed for optimum foam properties. Stable (S)/ Not Stable Bottle Pump Foam Humectant Comment Formula (NS) Foam Test Test Glycerin 1.0TCS/5DPG/15SXS/ S - + 2.5ALS/0.75CAPB/5GLY Glycerin 0.6TCS/5DPG/15SXS/ S - - 1.5ALS/0.75CAPB/10GLY Glycerin 0.3TCS/2DPG/15SXS/ S - - 0.75ALS/20GLY Sodium PCA 0.3TCS/2DPG/15SXS/ S - - 0.75ALS/1.0PCA Sodium PCA 0.3TCS/5DPG/15SXS/ S + + 0.75ALS/0.5PCA

EXAMPLE 4 Phase stability and foam performance attributed to ethoxylated additives--Ethoxylated additives provide an advantage because of a wide variety of raw materials and the ability to pre- determine properties by a judicious selection of the level of ethoxylation of the additive. It was ob- served that ethoxylated compounds having a rela- tively low level of ethoxylation (e. g., additives having an HLB about 4 to 8) were difficult to solubilize in the compositions, but gave excellent foam properties. Ethoxylated compounds having a higher level of ethoxylation (e. g., HLB about 8 to 17) were more easily solubilized, and also exhibited good to excellent foam properties. A mixture of ethoxylate compounds having an HLB about 12 also exhibited excellent foam properties demonstrated by the"+++"bottle foam test result. Stable (S)/ Bottle Pump Foam Ethoxylated Additive Comment Formula Not Stable Foam Test Test (NS) Polyoxyethylene HLB = 4.9 0.6TCS/5DPG/15SXS/1.5ALS/ S +++ ++ (2) stearyl ether 0.4BRIJ72 (BRIJ 72) Polyoxyethylene HLB = 4.9 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT (2) stearyl ether 0.6BRIJ72 (BRIJ 72) Polyoxyethylene HLB = 4.9 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT (2) stearyl ether 0.8BRIJ72 (BRIJ 72) Polyoxyethylene HLB = 4.9 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT (2) stearyl ether 1.0BRIJ72 (BRIJ 72) Polyoxyethylene HLB = 0.6TCS/5DPG/15SXS/1.5ALS/ S ++ + (21) stearyl ether 15.5 1.0BRIJ721 (BRIJ 721) BRIJ72/BRIJ721 Est. HLB 0.6TCS/5DPG/15SXS/1.5ALS/ S +++ ++ = 12.5 0.75CAPB/0.4BRIJ72/1.0BRIJ721 ARLASOLVE 200 HLB = 0.6TCS/5DPG/15SXS/1.5ALS/ S ++ ++ 15.7 0.75CAPB/ 1.0ARL200 Stable (S)/ Bottle Pump Foam Ethoxylated Additive Comment Formula Not Stable Foam Test Test (NS) PEG7 Glyceryl 1.0TCS/5DPG/15SXS/2.5ALS/ S - + Cocoate 0.75CAPB/0.2PEG7GC PEG7 Glyceryl 0.3TCS/5DPG/15SXS/0.75ALS/ S + + Cocoate 0.2PEG7GC PEG7 Glyceryl 0.3TCS/5DPG/15SXS/0.75ALS/S + + Cocoate 0.5PEG7GC JEECHEM GL-26 0.3TCS/5DPG/15SXS/0.75ALS/ S 0 0 0.2JCHMGL26 ARLACEL 80 HLB = 4.3 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/ 1.0ARL80 ARLACEL 80 HLB = 4.3 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/0.5ARL80 ARLACEL 80 HLB = 4.3 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/0.3ARL80 ARLACEL 80 HLB = 4.3 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/0.1ARL80 LAMESOFT 0.3TCS/5DPG/15SXS/0.75ALS/ S - - 0.1LMSFT

EXAMPLE 5 Phase stability and foam performance attributed to long-chain fatty materials--The com- positions of Example 5 show that cetyl alcohol gave outstanding performance in stabilizing foam. In some cases, foam generated in the bottle test lasted several days (vs. under an hour for the control).

The amount of cetyl alcohol incorporated into the base formula was 0.05% to 0.5%, by weight. Stearic acid provided improvement in foam properties, and was more difficult to solubilize. The fatty esters generally were more difficult to solubilize in the compositions. Stable (S) / Long-chain Not Stable Bottle Pump Foam Fatty Material Comment Formula (NS) Foam Test Test Cetyl alcohol 0.6TCS/5DPG/15SXS/ S + + 1.5ALS/0.5CAPB/0.05CETOH Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS/ S ++ ++ 0.5CAPB/0.2CETOH Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS/ S ++ ++ 0.5CAPB/0.2CETOH Cetyl alcohol 0.6TCS/5DPG/15SXS/2.5ALS/ S ++ ++ 0.75CAPB/0.5CETOH Cetyl alcohol 0.3TCS/5DPG/0.75ALS/ S ++ ++ 0.1CETOH Cetyl alcohol 1.0TCS/5DPG/15SXS/2.5ALS/ S +++ ++ 0.75CAPB/0.3CETOH Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS S - + 0.5CAPB/0.5CETOH Stearic Acid 1.0TCS/5DPG/15SXS/2.5ALS/ S + + 0.75CAPB/0.15STAC Stearic Acid 1.0TCS/5DPG/15SXS/2.5ALS/ NS NT NT 0.75CAPB/0.2STAC Stearic Acid 0.6TCS/5DPG/15SXS/1.5ALS/ S - + 0.75CAPB/0.2STAC Stable (S) / Long-chain Not Stable Bottle Pump Foam Fatty Material Comment Formula (NS) Foam Test Test Isopropyl 1.0TCS/5DPG/15SXS/2.5ALS/ NS NT NT Myristate 0.75CAPB/0.2IPM Decyl Oleate 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/1.0DCYLOL Decyl Oleate 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/0.5DCYLOL Decyl Oleate 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT 0.75CAPB/1.0DCYLOL Cetearyl 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT Isononanate 0.75CAPB/1.0CETISONON Cetearyl 0.6TCS/5DPG/15SXS/1.5ALS/ NS NT NT Isononanate 0.75CAPB/0.5CETISONON Cetearyl 0.6TCS/5DPG/15SXS/1.5ALS/NS NT NT Isononanate 0.75CAPB/0.25CETISONON

EXAMPLE 6 Phase stability and foam performance attributed to other additives--ISML (isostearyl- morpholine lactate) was a useful additive in these tests. Petrolatum was difficult to solubilize in the base formulae of the invention. Petrolatum also is a component of at least two recently introduced commercial antimicrobial hand wash products (see Table 2 below). Commercial product E also contains dimethicone. Stable (S) / Bottle Pump Other Comment Formula Not Stable Bottle Pump Foam Additives (NS) Test Test MACKERNIUM 0.3TCS 5DPG/15SXS/0.75ALS/0.1MAC1216 S - + 1216 MACKERNIUM 0.3TCS 5DPG/15SXS/0.75ALS/0.5SFES S - + SFES OLIVEM 400 0.3TCS/5DPG/15SXS/0.75ALS/0.5OL400 S - + PROMIDIUM CC 0.6TCS/5DPG/15SXS/1.5ALS/1PCC S - 0 PROMIDIUM CO 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/0.4PCO S NT NT Mineral Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/0.5MO NS NT NT Mineral Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/0.25MO NS NT NT Mineral Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/0.13MO NS NT NT Mineral Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/0.05MO NS NT NT Mineral Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT 0.025MO Petrolatum 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ NS NT NT 0.05PETR Petrolatum 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/0.1PETR NS NT NT ISML 0.3TCS/5DPG/15SXS/0.75ALS/0.1ISML S + + ISML 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/ S - + 0.21ISML Sr (OAc)2 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/ NS NT NT 0.2Sr(OAc)2 Sr (OAc)2 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/ NS NT NT 0.2Sr(OAc)2 Table 2--Ingredients Statements for Commercial Antimicrobial Hand Washer Commercial Product E commercial Product F (ingredients statement on label)) (ingredients statement on label) ACTIVE INGREDIENT: Triclosan ACTIVE INGREDIENT: 0.25% TRICLOSAN OTHER INGREDIENTS: INACTIVE INGREDIENTS: WATER WATER SODIUM LAURETH SULFATE PETROLATUM COCAMIDOPROPYL BETAINE SODIUM LAUROYL SARCOSINATE PETROLATUM SODIUM LAUROAMPHOACETATE DIMETHICONE AMMONIUM LAURYL SULFATE LAURIC ACID AMMONIUM LAURETH SULFATE DECYL GLUCOSIDE LAURIC ACID ACRYLATES/C10-30 ALKYL ACRYLATE CROSSPOLYMER TRIHYDROXYSTEARIN HYDROXYPROPYL METHYLCELLULOSE GUAR HYDROXYPROPYLTRIMONIUM CHLORIDE TRIETHANOLAMINE CITRIC ACID FRAGRANCE SODIUM BENZOATE DMDM HYDANTOIN DISODIUM EDTA POLYQUATERNIUM-39 FRAGRANCE POLYQUATERNIUM-7 GLYCERIN TETRASODIUM EDTA PEG-90 STEARATE D&C ORANGE NO. 4 METHYLCHLOROISOTHIAZOLINONE FD&C RED NO. 40

EXAMPLE 7 Phase stability and foam performance attributed to combinations of additives--Several tested compositions, especially those containing a combination of cetyl (or cetearyl) alcohol, stearic acid, and/or glycerin, exhibited excellent foam stability. Stable (S) / Long-chain Not Stable Bottle Pump Foam Comment Formula (NS) Foam Test Test Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ S +++ ++ 5GLY/0.2CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ S +++ ++ 10GLY/0.2CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S +++ ++ 10GLY/0.2CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S +++ ++ 10GLY/0.2CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT 10GLY/0.3CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT 10GLY/1.0CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT 10GLY/0.4CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S +++ ++ 10GLY/0.2CETOH Glycerin/Stearic Acid 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT 10GLY/0.15STAC Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S +++ ++ Acid/Cetyl Alcohol 10GLY/0.05STAC/0.15CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13ALS/0.37CAPB/ S +++ ++ Acid/Cetyl Alcohol 5GLY/0.05STAC/0.15CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ S +++ ++ Acid/Cetyl Alcohol 10GLY/0.025STAC/0.15CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13ALS/0.37CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.05STAC/0.15CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.05STAC/0.2CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13ALS/0.37CAPB/ NS NT NT Acid/Cetyl Alcohol 7.5GLY/0.2CETOH/0.05STAC Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.2CETOH/0.05STAC Stable (S) / Not Stable Bottle Pump Foam Comment Formula (NS) Foam Test Test Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.0ALS/0.5CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.15CETOH/0.05STAC Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.1STAC/0.2CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.2CETOH/0.05STAC Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ NS NT NT Acid/Cetyl Alcohol 10GLY/0.2CETOH/0.05STAC SLES2/Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13SLES2/0.37CAPB/ S +++ ++ Acid/Cetyl Alcohol 10GLY/0.05STAC/0.15CETOH SLES2/Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ S +++ ++ Acid/Cetyl Alcohol 10GLY/0.05STAC/0.15CETOH SLES2/Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13SLES2/0.37CAPB/ NS NT NT Acid/Cetyl Alcohol 7.5GLY/0.05STAC/0.2CETOH Cetyl Alcohol/PROMIDIUM CC 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S +++ ++ 0.3CETOH/0.5PCC Cetyl Alcohol/PROMIDIUM CC 0.6TCS/5DPG/15SXS/0.5CETOH/1.5PCC NS NT NT Stearic Acid/Cetyl Alcolol 0.6TCS/5DPG/1.5ALS/0.75CAPB/ S +++ ++ 0.15CETOH/0.025STAC Stearic Acid/Cetyl Alcolol 0.6TCS/5DPG/1.5ALS/0.5CAPB/ S +++ ++ 0.15CETOH/0.05STAC Sodium PCA/Glycerin 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ S - - 10GLY/.5PCA JAGHP60/MACKALINE SFES 0.3TCS/5DPG/15SXS/0.75ALS/ S + + 0.5JACGP60/0.5SFES Sodium PCA/JAGHP60 0.3TCS/5DPG/15SXS/0.75ALS/ S 0 - 0.05JAGHP60/1.0PCA Stable (S)/ Not Stable Bottle Pump Foam Combinations Formula (NS) Foam Test Test JAGHP60/OLIVEM 400 0.3TCS/5DPG/15SXS/0.75ALS/ S - + 0.05JAGHP60/0.5OLIVM400 Lauricidin/MACKALINE SFES 0.3TCS/5DPG/15SXS/0.75ALS/0.5SFES/ NS NT NT 0.5LRCDN Polyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.25PQ10/ S +++ ++ Alcohol/Glycerin/NaPCA 0.1CETOH/3GLY/1.5NaPCA LAMESOFT/Glycerin/NaPCA/ 0.4TCS/5DPG/15SXS/0.75ALS/0.5LAMSFT/ S +++ ++ JAGHP60 2.5GLY/1.5NaPCA/0.04JAGHP60/0.1CETOH NATROSOL HEC/PEG-75 0.3TCS/5DPG/15SXS/0.75ALS/ S ++ + Lanolin/PPG-5-Ceteth20 0.5NTSLHEC/0.5PEG-75LAN/0.5PPG5CET20 NATROSOL HEC/Sunflower oil 0.3TCS/5DPG/15SXS/0.75ALS/ S - 0 0.05NTSLHEC/1.0SUNFLWR NATROSOL HEC/GlycerinPOE 0.3TCS/5DPG/15SXS/0.75ALS/ S - - 0.05NTSLHEC/0.5GLYPOE NATROSOL HEC/NaPCA 0.3TCS/5DPG/15SXS/0.75ALS/ S - - 0.05ntslhec/1.0NaPCA JAGHP60/Sunflower oil 0.3TCS/5DPG/15SXS/0.75ALS/ S - + 0.04JAGHP60/1.0SUNFLWR JAGHP60/Sunflower oil/VitE 0.3TCS/5DPG/15SXS/0.75ALS/ S - + 0.03JAGHP60/1.0SUNFLWR/0.01VitE JAGHP60/NaPCA 0.3TCS/5DPG/15SXS/0.75ALS/J S + + 0.05JAGHP60/0.5NaPCA NaPCA/MACKPRO WLW/JAGHP60/ 0.3TCS/5DPG/15SXS/0.75ALS/ S + - Aloe Vera 0.04JAGHP60/1.0NaPCA/0.5WLW/0.01AV NaPCA/ISML/JAGHP60/Aloe 0.3TCS/5DPG/15SXS/0.75ALS/ S ++ + Vera 0.04JAGHP60/1.0NaPCA/0.5ISML/0.01AV NaPCA/MACKALENE 1216/ 0.3TCS/5DPG/15SXS/0.75ALS/ S + + JAGHP60/VitaminEOAc 0.04JAGHP60/1.0NaPCA/0.5M1216/0.01Vit E Stable (S)/ Not Stable Bottle Pump Foam Combinations Formula (NS) Foam Test Test NaPCA/Glycerin/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/ S +++ ++ Alcohol 1.5NaPCA/ 3.8GLY/0.1CETOH/0.1ALOE NaPCA/Polyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.5NaPCA/ S +++ NT Alcohol/LAMESOFT 0.25CQSC240C/0.1CETOH/0.5LMSFT NaPCA/Polyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.5NaPCA/ S +++ NT Alcohol/VARISOFT 0.25CQSC24OC/0.1CETOH/0.5VRSFT NaPCA/Polyquat10/Cetyl 0.3TCS/5DPG/15SXS/0.75ALS/0.5LMSFT/ S ++ ++ Alcohol/Glycerin/LAMESOFT 0.5NaPCA/3GLY/0.1CETOH/0.25CQSC240C NaPCA/Polyquat10/Cetyl 0.3TCS/5DPG/15SXS/0.75ALS/1.0NaPCA/ S ++ ++ Alcohol/Glycerin 3GLY/0.1CETOH/0.25CQSC240C

EXAMPLE 8 Antimicrobial Performance in Time Kill Tests. Results for antimicrobial efficacy are summarized in the following table--Unless otherwise indicated, the values are for log reduction of Serratia marcescens at 30 seconds. Values for"Sa," "Ec,"and"Kp"refer to Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, respec- tively, at 30 seconds. The log reduction value for the test composition appears first, followed by the log reduction value for an appropriate control sample (in the table below,"// (cna)" means control not available). A log reduction value within about 1 log of the control sample is considered highly efficacious. Values for Serratia marcescens vary somewhat, between about log 2 to >log 4 reduction, for control samples. Description Formula Tima Kill Results Control Formula 1 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB >4.14 (Sa30) />4.60 (Ec30) Control Formula 2 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB 4.73/--- Control Formula 3 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB 2.74/-- Control Formula 4a 0.3TCS/5DPG/15SXS/0.75ALS >4.91/--- Control Formula 4b 0.3TCS/5DPG/15SXS/0.75ALS >4.86/--- Control Formula 4c 0.3TCS/5DPG/15SXS/0.75ALS 3.15/--- Control Formula 4d 0.3TCS/5DPG/15SXS/0.75ALS >4.83/--- Control Formula 4e 0.3TCS/5DPG/15SXS/0.75ALS 3.17/--- Control Formula 4f 0.3TCS/5DPG/15SXS/0.75ALS >4.90 (Sa)/--- >5.00 (Ec)/ --- 4.47 (Kp)/ --- 2.97 (Sm)/ --- Control Formula 4g 0.6TCS/5DPG/15SXS/1.5ALS/1PCC 4.28 Sodium PCA/JAGHP60 0.3TCS/5DPG/15SXS/0.75ALS/0.05JAGHP60/ 2.99/--- (used as an "approximate" 1.0PCA control) (Control 5) Primary Surfactants Sodium Lauryl Ether 1.0TCS/5DPG/15SXS/2.5SLES2/0.75CAPB >4.69 (Sa30) /4.54 (Ec30) Sulfate (2-mole) Ammonium Cocyl 1.0TCS/5DPG/15SXS/2.5ACI/0.75CAPB >4.69 (Sa30) /4.29 (Ec30) Isethionate // (cna) Polymers PVP K30 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/ >4.14 (Sa) />4.60 (Ec) // >4.14/ 0.1PVPK30 >4.60 (1) JAGUAR HP-60 0.3TCS/5DPG/15SXS/0.75ALS/0.1HP60 >4.86/>4.86 (4b) CELQUAT SC-230M 0.3TCS/5DPG/15SXS/0.75ALS/0.1CQSC230M >4.86/>4.86 (4b) CELQUAT SC-230M 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ 4.38/4.73 (2) 0.5CQSC230M CELQUAT H-100 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ 4.38/4.73 (2) 0.5CQH100 Description Formula Time kill Results NATROSOL 250 HHR 0.3TCS/5DPG/15SXS/0.75ALS/0.05NATSOL250HHR >4.86/>4.86 (4b) CARBOWAX 540 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ 4.73/>4.73 (4d) 1.0CWAX540 CARBOWAX 900 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ 4.73/>4.73 (4d) 1.0CWAX900 FLEXAN 130 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ 4.73/>4.73 (4d) 1.0FLEX130 Protein Derivatives MACKPRO WWP 0.3TCS/5DPG/15SXS/0.75ALS/1.0WWP 3.41/>4.91 (4a) MACKPRO WWP 0.3TCS/5DPG/15SXS/0.75ALS/0.5WWP 3.95/>4.91 (4a) MACKPRO NLW 0.3TCS/5DPG/15SXS/0.75ALS/0.2WWP >4.86/>4.86 (4b) Silicone Derivatives Dimethicone Propyl PG 0.3TCS/5DPG/15SXS/0.75ALS/0.2DIMETHPGB >4.86/>4.86 (4b) Betaine Stearyl Methicone 0.3TCS/5DPG/15SXS/0.75ALS/0.05STMETH 3.60/>4.91 (4a) Dow Corning 193 0.3TCS/5DPG/15SXS/0.75ALS/0.2DC193 4.41/>4.86 (4b) Humectants Glycerin 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/5GLY >4.14 (Sa) />4.60 (Ec) //>4.14/ >4.60 (1) Sodium PCA 0.3TCS/2DPG/15SXS/0.75ALS/0.5PCA >4.86/>4.86 (4b) Ethoxylated Derivative Polyoxyethylene (2) 0.6TCS/5DPG/15SXS/1.5ALS/0.4BRIJ72 2.31/4.28 (4g) stearyl ether (BRIJ 72) Polyoxyethylene (21) 0.6TCS/5DPG/15SXS/1.5ALS/1.0BRIJ721 2.33/4.28 (4g) stearyl ether (BRIJ 721) ARLASOLVE 200 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/ 1.0ARL200 Description Formula Time Kill Results PEG7 Glyceryl Cocoate 0.3TCS/5DPG/15XSX/0.75ALS/0.5PEG7GC >4.86/>4.86 (4b) LAMESOFT 0.3TCS/5DPG/15SXS/0.75ALS/0.1LMSFT 3.73/>4.91 (4a) Long-chain Fatty Materials Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/0.05CETOH 2.56/2.74 (3) Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/0.2CETOH 2.70/2.74 (3) Cetyl alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/0.1CETOH 2.65/2.74 (3) Stearic Acid 1.0TCS/5DPG/15SXS/2.5ALS/0.7CAPb/0.2StAc >4.14 (Sa)/>4.60 (Ec) //>4.14/ >4.60(3) Lipid-like Materials Canola Oil 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/10GLY/ 2.96/3.15 (4c) 0.1CANOL Other Emollients MACKERNIUM 1216 0.3TCS/5DPG/15SXS/0.75ALS/0.1MAC1216 >4.86/>4.86 (4b) PROMIDIUM CC 0.6TCS/5DPG/15SXS/1.5ALS/1PCC 4.28/4.28 (4g) ISML 0.3TCS/5DP/15SXS/0.75ALS/0.1ISML 4.76/>4.86 (4b) ISML 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/0.21SML >4.69 (Sa30)/4.06 (Ec30) //(cna) Sr(OAc)2 1.0TCS/5DPG/15SXS/2.5ALS/0.75CAPB/ >4.69 (Sa30)/3.24 (Ec30) 0.2Sr(OAc)2 //(cna) JEECHEM GL-26 0.3TCS/5DPG/15SXS/0.75ALS/0.2JCHMGL26 >4.86/>4.86 (4b) Combinations Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/5GLY/ 2.77/2.74 (3) 0.2CETOH Glycerin/Getyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/10GLY/ 3.00/2.74 (3) 0.2CETOH Glycerin/Cetyl Alcohol 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/10GLY/ 2.38/3.15 (4c) 0.2CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.5ALS/0.75CAPB/10GLY/ 2.46/3.15 (4c) Acid/Cetyl Alcohol 0.05STAC/0.15CETOH Glycerrin/Stearic 0.6TCS/5DPG/15SXS/1.13ALS/0.37CAPB/5GLY/ 2.41/3.15 (4c) Acid/Cetyl Alcohol 0.05STAC/0.15CETOH Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13ALS/0.37CAPB/10GLY/2.70/3.15 (4c) Acid/Cetyl Alcohol 0.05STAC/0.15CETOH Descritpion Formula Time Kill Results SLES2/Glycerin/Stearic 0.6TCS/5DPG/15SXS/1.13SLES2/037CAPB/ 2.30/3.15 (4c) Acid/Cetyl Alcohol 10GLY/0.05STAC/0.15CETOH SLES2/Glyceri/Stearic 0.6TCS/5DPG/15SXS/1.5SLES2/0.5CAPB/10GLY/ 2.31/3.15 (4c) Acid/Cetyl Alcohol 0.05STAC/0.15CETOH Stearic Acid/Cetyl 0.6TCS/5DPG/15SXS/1.5ALS/0.5CAPB/ 2.59/3.15 (4c) Alcohol 0.15CETOH/0.05STAC JAGHP60/MACKALINE SFES 0.35CS/5DPG/15SXS/0.75ALS/0.05JAGHP60/ 1.22/2.99 (5) 0.5SFES JAGHP60/OLIVEM 400 0.3TCS/5DPG/15SXS/075ALS/0.05JAGHP60/ 1.37/2.99 (5) 0.5OLIVM400 Sodium PCA/JAGHP60 0.3TCS/5DPG/15SXS/0.75ALS/0.05JAGHP60/ 2.99/2.99 (5) 1.0PCA Polyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/0.25CQS 4.30/>4.83 (4d) lcohol/Glycerin/NaPCA/ C240C/0.1CETOH/3GLY/1.5NaPCA/0.1ALOE Aloe Vera LAMESOFT/Glycerin/NaPCA/ 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/ 4.53/>4.83 (4d) JAGHP60/Aloe Vera 0.5LAMSFT/2.5GLY/1.5NAPC/0.04JAGHP60/ 0.1ALOE NATROSOL HEC/PEG-75 0.3%CS/5DPG/15SXS/0.75ALS/0.05NTSLHEC/ 0.79/2.99 (5) Lanolin/PPG-5-Ceteth20 0.5PEG-75LAN/0.5PPG5CET20 NATROSOL HEC/Sunflower 0.3TCS/5DPG/15SXS/0.75ALS/0.05NTSLHEC/ 1.22/2.99 (5) oil 1.0SUNFLWR NATROSOL HEC/GlycerinPOE 0.3TCS/5DPG/15SXS/0.75ALS/0.05NTSLHEC/ 1.53/2.99 (5) 0.5GLYPOE NATROSOL HEC/NaPCA 0.3TCS/5DPG/15SXS/0.75ALS/0.05NTSLHEC/ 2.16/2.99 (5) 1.0NaPCA JAGHP60/Sunflower oil 0.3TCCS/5DPG/15SXS/0.75ALS/0.04JAGHP60/ 1.43/2.99 (5) 1.0SUNFLWR JAGHP60/SunflowerOil/VitE 0.3TCS/5DPG/15SXS/0.75ALS/0.03JAGHP607 2.16/2.99 (5) 1.0SUNFLWER/0.01VitE JAGHP60/NaPCA 0.3TCS/5DPG/15SXS/0.75ALS/0.05JAGHP60/ 3.95/>4.91 (4a) 0.5NaPCA Description Formula Time Kill Results NaPCA/MACKPROWLSW/JAGHP60/ 0.35TCS/5DPG/15SXS/0.75ALS/0.04JAGHP60/ 3.14/3.17 (4e) Aloe Vera 1.0NaPCA/0.5WLW/0.01AV NaPCa/ISML/JAGHP60/Aloe 0.3TCS/5DPG/15SXS/0.75ALS/0.04JAGHP60/ 3.00/3.17 (4e) Vera 1.0NaPCA/0.5ISML/0.01AV NaPCa/Mackalene1216/ 0.3TCS/5DPG/15SXS/0.75ALS/0.04JAGHP60/ 1.88/3.17 (4e) JAGHP60/VitaminEOAc 1.0NaPCA/0.5M1216/0.1VitE NaPCA/Glycerin/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPE/ 4.58/>4.83 (4d) Alcohol/Aloe Vera 1.5NaPCA/3.8GLY/0.1CETOH/0.1ALOE NaPCA/Polquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/>4.73/>4.73 (4d) Alcohol/LAMESOFT/Aloe 0.5NaPCA/025CQSC240C//0.1CETOH/0.5LMSFT/ Vera 0.1ALOE NaPCA/POlyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/ 4.25/>4.73 (4d) Alcohol/VARISOFT/Aloe 0.5NaPCA/0.25CQSC240C//0.1CETOH/0.5VRSFT/ Vera 0.1ALOE NaPC/Polyquat10/Cetyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/ >4.90 (Sa) />4.90 (4f) Alcohol/Glycerin/Aloe 1.0NaPCA/3GLY/0.1CETOH/0.25CQSC240C/ >5.00 (Ec) />5.00 (4f) Vera 0.1ALOE 4.65 (Kp) /4.47 (4f) 2.94 (Sm) /2.97 (4f) NaPCa/Polyquat10/Cetearyl 0.4TCS/5DPG/15SXS/0.75ALS/0.75CAPB/ >4.90 (Sa) />4.90 (4f) Alcohol/Glycerin/Aloe 0.5LMSFT/0.5NaPCA/3GLY/0.1CETOH/ >5.00 (Ec) />5.00 (4f) Vera 0.25CQSC240C/0.1ALOE 4.70 (Kp) / 4.47 (4f) 2.85 (Sm) /2.97 (4f)

EXAMPLE 9 Antimicrobial Performance Tests (Broad Spectrum Efficacy)--The following embodiment of the present invention was tested: COMPOSITION A (by weight): 0.46 TCS/- 5DPG/15SXS/0.75CAPB/0.129 Disodium Phos- phate/0.066 Citric Acid, buffer (pH-6)/0. 1 Cetyl Alcohol/0.05 fragrance/1 Sodium PCA/2.97GLY/0.25 Polyquaternium-100/0.1 Aloe Vera Gel/0.15 Methyl Paraben/0.05 Propylparaben/0.00005 FD&C Red #4/0. 000025 Yellow &num 5.

Time kill tests were performed to compare Composition A of the present invention to several commercially available Health Care Personnel Hand Wash products (i. e., HCPHW-E, F, G, H, I, J) and to several commercially available retail antibacterial hand wash products. Three nonmedicated retail liquid hand soaps also were tested for comparison.

The tests evaluated efficacy against a broad spectrum of twenty-four different microorganisms.

Test organisms were selected to represent both transient and resident organisms, Gram negative bacteria (such as Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium), and Gram positive bacteria (such as Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes). The compositions were tested with sampling taking place at 30 seconds and 1 minute.

The test organisms represented a broad spectrum of both Gram positive and Gram negative organisms commonly associated with nosocomial infec- tions. For the health care products, five addition- al test organisms were added as a result of a health care survey, including several antibiotic resistant strains of bacteria. The following Time Kill Summary charts summarize bacterial kill results for Composition A vs. several Health care Personnel Hand wash Products.

The Time Kill Summary charts summarize data for both Health care Personnel Hand washes and retail liquid hand soaps, and includes a number of organisms of the total tested that were reduced by greater than 3,2, or 1 log within 30 seconds.

Antimicrobial potential can be classified based on a product's ability to reduce the number of organisms in logarithms. A product that is unable to achieve a 1 log reduction shows minimal activity against that specific organism. A one log reduction is con- sidered moderate activity, whereas a greater than 2 or 3 log reduction is considered strong antibacter- ial activity in vitro.

The summarized results demonstrate a sig- nificantly superior efficacy for Composition A versus the twenty-four test organisms (30 second time-kill). Composition A performed significantly better than each of the commercially available Health Care Personnel Hand Wash products tested (i. e., HCPHW-E through J) at reducing the number and type of microorganisms encountered in health care settings. Further, compared to the leading liquid hand soaps and health care products, Composition A

was superior at reducing more types of test organisms by greater than 3 logs within 30 seconds.

Composition A reduced 19 of 24 organisms tested by greater than 3 log units within 30 seconds. The closest comparative composition, HCPHW-I, reduced 16 of 24 organisms greater than 3 log units. The re- maining comparative compositions showed moderate to minimal antimicrobial activity.

Time Kill Summary-I<BR> Log Reduction Commercial Commercial Commercial Commercial Commercial Composition Product Product Product Product Products Test Organisms A HCPHW-E P|HCPHW-F HCPHW-G HCPHW-E HCPHW-I 30 1 30 1 30 1 30 1 30 1 30 1 Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Staphylococcus aureus 5.07 >5.17 1.31 2.14 1.05 1.43 2.98 4.38 1.75 2.55 2.94 4.23 (ATCC 6538) Staphylococcus 3.35 4.41 0.46 0.47 0.23 0.42 0.38 0.65 0.78 1.00 5.02 4.66 ]epidermis (ATCC 12228) Stphylococcus aureus 0.98 1.93 0.05 0.17 0.10 0.12 0.31 0.50 0.19 0.23 1.73 2.80 MRSA (ATCC 33592) Streptococcus >3.07 >3.07 >3.07 >3.07 >3.07 >3.07 >3.07 >3.07 >3.07 >3.07 1.91 1.55 pneumoniae (ATCC 6303) Streptococcus Pyogenes >4.11 >4.11. 4.01 >4.11 3.80 >4.11 >4.98 >3.98 >4.11 >4.11 >4.97 >3.97 (ATCC 19615) Pseudomonas aeruginosa >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 >5.23 (clinical isolate) Pseudomonas aeruginosa >5.25 >5.25 >5.25 >5.25 >5.25 >5.25 >5.25 >5.25 >5.25 >5.25 >4.21 >4.21 (ATCC 9027) Klebsiella pneumoniae >5.07 >5.07 2.26 2.76 4.52 >5.07 5.02 >5.07 3.45 4.18 >4.27 >4.27 (ATCC 11296) Burkholderia cepacia >4.92 >4.92 0.00 0.05 1.56 4.59 2.08 4.92 0.05 0.47 2.42 1.65 (ATCC 25416) Serratia marcescens 3.96 >5.47 0.16 0.27 0.02 0.07 0.15 0.51 0.18 0.23 >4.59 >4.59 (ATCC 14756) Time Kill Summary-I<BR> Log Reduction Commercial Commercial Commercial Commercial Commercial Composition Product Product Product Product Products Test Organisms A HCPHW-E HCPHW-F HCPHW-G HCPHW-H HCPH-I 30 1 30 1 30 1 30 1 30 1 30 1 Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Shigella sonnei 4.22 >5.23 3.75 5.03 0.63 1.63 2.33 5.23 3.65 >5.23 4.18 >5.36 (ATCC 11060) Salmonella choleraseuis >5.27 >5.27 1.83 3.66 0.67 1.32 5.07 >5.27 1.31 2.91 >5.64 >5.64 (ATCC 13076) Salmonella choleraseuis >5.20 >5.20 2.02 >5.20 0.93 3.56 5.04 >5.20 3.14 >5.20 >4.59 >4.59 (typhi) (ATCC 6539) Stenotrophomonas >5.11 >5.11 4.95 4.89 >5.11 4.95 >5.11>5.11 >5.11 >5.11 >4.97 >4.97 maltophilia (ATCC 13637) Enterobacter aerogenes 2.61 >5.23 0.43 0.63 0.30 0.45 0.56 1.46 0.60 0.73 2.60 3.78 (ATCC 13048) Escherichia coli >5.14 >5.14 0.72 1.06 0.66 0.96 1.10 1.89 2.08 2.70 >4.32 >4.32 (ATCC 11229) Escherichia coli 2.51 >4.98 0.96 2.71 0.45 1.29 2.29 >4.98 1.32 2.65 >4.22 >4.22 O:157H:& (ATCC 43888) Citrobacter freundii 3.46 >4.88 0.64 1.56 0.97 1.50 4.66 >4.88 0.44 0.61 >4.28 >4.28 (ATCC 43864) Enterococcus faecium 3.37 4.26 0.53 1.38 0.32 0.72 2.67 4.05 0.72 1.95 0.30 0.54 (ATCC 51559) Enterococcus faecalis >5.98 >5.98 0.78 2.30 1.08 2.06 4.23 5.41 1.08 2.38 0.75 1.20 (ATCC 51299) Time Kill Summary-I<BR> Log Reduction Commercial Commercial Commercial Commercial Commercial Composition Product Product Product Product Products jTest Organisms A HCPHW-E HCPHW-F HCPHW-G HCPHW-H HCPHW-I 30 1 30 1 30 1 30 1 30 1 30 1 Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Sec. Min. Clostridium difficile 3.44 3.56 >4.14 >4.14 >4.14 >4.14 >4.14 >4.14 >4.14 >4.14 >4.14 >4.14 (ATCC 9689) Candida albicans 1.78 3.05 0.44 1.08 0.09 0.33 0.20 1.08 0.37 0.83 2.07 2.63 (ATCC 10231) Candida tropicalis 2.01 2.85 1.12 2.12 0.25 0.42 0.28 0.79 1.32 2.36 >4.76 >4.76 (ATCC 750) Shodotorula rubra >5.14 >5.1 2.05 2.68 2.60 4.32 4.91 >5.14 2.52 3.33 >4.74 >4.74 (ATCC 9449) Time Kill Summary--II @ 30 seconds Composition A Formula AA-1 Retail-CS nonmed.) 19 organisms>3 log or 99.9% 16>3 log 2>3 log 2 organisms>2 log or 99% 2>2 log 0>2 log 2 organisms>1 log or 90% 1>1 log 1>1 log 1 organism<1 log 0<1 log 16<1 log HCPHW-I HCPHW-J Retail-EAB 16 organisms>3 log or 99.9% 8>3 log 1>3 log 4 organisms>2 log or 99% 2>2 log 2>2 log 2 organisms>1 log or 90% 2>1 log 1>1 log 2 organisms<l log 7<1 log 15<1 log HCPHW-G Retail-SAB Retail-SSA 10 organisms>3 log or 99.9% 5>3 log 1>3 log 5 organisms>2 log or 99% 1>2 log 2>2 log 1 organism>1 log or 90% 1>1 log 1>1 log 8 organisms<l log 12<1 log 15<1 log HCPHW-H Retail-KAB Retail-SSM (nonmed.) 9 organisms>3 log or 99.9% 3>3 log 1>3 log 2 organisms>2 log or 99% 1>2 log 0>2 log 5 organisms>1 log or 90% 6>1 log 1>1 log 8 organisms<1 log 9<1 log 17<1 log HCPHW-E Retail-PAB Retail-ILS (nonmed.) 7 organisms>3 log or 99.9% 3>3 log 1>3 log 3 organisms>2 log or 99% 1>2 log 1>2 log 3 organisms>1 log or 90% 5>1 log 1<1 log 11 organisms<1 log 10<1 log 16<1 log HCPHW-F Retail-SSP 7 organisms>3 log or 99.9% 2>3 log 1 organism>2 log or 99% 1>2 log 3 organisms>1 log or 90% 1>1 log 13 organisms<l log 15<1 log FORMULA AA-1 is a retail antibacterial formula produced in accordance with U. S. Patent No. 6,107,261.

EXAMPLE 10 Antimicrobial Performance Tests (Health Care Personnel Hand Wash Test)--The FDA issued a tentative final monograph (June 17,1994) setting forth a health care personnel hand wash method to determine the effectiveness of antibacterial cleansing products. The following embodiment of the present invention was tested using this method: Composition B (by weight): 0.04 TCS/- 5DPG/15SXS/0.75ALS/0.75CAPB/0.129 Disodium Phosphate/0.066 Citric Acid, buffer (pH=6)/0. 1 Cetyl Alcohol/0.05 fragrance/- 1.0 Sodium PCA/2.97GLY/0.25 Polyquatern- ium-100/0.1 Aloe Vera Gel/0.15 Methyl Paraben/0.05 Propylparaben/0.00005 FD&C Red #4/0. 000025 Yellow #5.

The in-use antibacterial efficacy of Com- position B was determined by a health care personnel hand wash study. The study was performed according- to the current revision of ASTM E-1174-00, Standard Test Method for Evaluation of the Effectiveness of Health Care Personnel or Consumer Hand wash Formula- tions, incorporated herein by reference. The revi- sion to the test method provides procedures to assure adequate rapid neutralization of the antimi- crobial in the hand wash formulation. A neutralizer was incorporated at both sampling points. The study is designed to measure the reduction of transient microbial flora following routine hand washing with

an antibacterial product. In this study, a broth culture of Serratia marcescens ATCC 14756 was used as an artificial contaminant bacteria on the hands.

Activity was measured by comparing the microbial counts of the marker organism removed after a single use of the test composition to the baseline number, i. e., the number of organisms recovered from contam- inated, unwashed hands. Additional comparisons were made following the tenth wash of a multiple wash procedure.

Prior to each of the eleven washes, the hands were artificially contaminated with S. marcescens. In addition to testing Composition B, HCPHW-I also was included in the study. A suffi- cient number of subjects fulfilling the study criteria were preenrolled to ensure the required number of subjects (45), 30 for Composition B and 15 for HCPHW-I. During a one-week wash out period, the subjects refrained from using antimicrobial-contain- ing products. On the test day, subjects'hands were contaminated with S. marcescens and a baseline sam- pling was performed. Following washing with the test composition, and following treatments 1 and 10, the subjects'hands were sampled for a post-treat- ment count. The sampling fluid was enumerated for recovery of S. marcescens. Results from the Health Care Personnel Hand Wash study were evaluated by comparing bacteria counts recovered from the hands following product treatment vs. the baseline counts.

The bacteria counts were converted into log10 counts.

The log counts of each subject's left and right hand were averaged. The following log10 reductions were observed:

Product Description WASH 1 WASH 10 Composition B 3.47 3.58 2) HCPHW-I 2.50 3.78 2) No statistical difference between the test compositions.

For antiseptic hand wash or health care personnel hand wash products, as proposed in the Tentative FDA Monograph (Health care Antiseptic Drug Products), the following criteria should be met: a 2 log reduction of the marker organism on each hand within 5 minutes after the first wash and a 3 log reduction of the marker organism on each hand within 5 minutes following the tenth wash.

Composition B met and surpassed both of these criteria. When compared to HCPHW-I, Composi- tion B performed significantly better with respect to reducing the concentration of the marker organism after one wash, and was equally effective following the tenth wash. The demonstrated log reductions illustrate that the present compositions are effec- tive as Health Care Personnel Hand wash products.

EXAMPLE 11 Repeat Application Soap Chamber Test--A soap chamber irritation test was performed to deter- mine the mildness of Composition A vs. several com- mercially available Health Care Personnel Hand Wash products. The tests showed that: (a) all test com-

positions were significantly less irritating than the positive control, i. e., a dilute solution of sodium lauryl sulfate (SLS), and (b) ranking prod- ucts from highest irritation potential to lowest is: SLS>HCPHW-E>HCPHW-H>HCPHW-G>HCPHW-F>Compositi on A>Negative Control.

Methodology Twelve male and female subjects between the ages of 18 and 65, who were in good health, were enrolled in the test. Dilute solutions of all test compositions were made each day of patching.

Patches were totally occlusive chambers, 12 mm in diameter, applied to the volar forearm for a total of six days. Expert visual gradings, using a four- point scale for erythema, scaling, and fissuring were used as the objective measure of observation for this study. Grading was performed at baseline (i. e., when panelists were enrolled), 30 minutes after patch removal on days one to six, and at 24 hours on days one to six. A maximum for each tested characteristic was established as a"3"score.

Summary The rating for the commercially available hand wash products, from highest irritation potential to the lowest, was: Positive Control> HCPHW-E>HCPHW-H>HCPHW-G>HCPHW-F>Composition A>Negative Control. Significant differences were noted overall between HCPHW-E and HCPHW-H, compared to HCPHW-G, HCPHW-F, and Composition A. Directional

differences existed between Composition A, HCPHW-F, and HCPHW-G, with Composition A demonstrating the lowest irritation potential as measured under the conditions of the test.

Table 1--Professional Products Statistical Groupings Mean Summary Statistical Grouping Product Score Positive Control 11.8 I HCPHW-E 3.9 II III HCPHW-H 2.2 II III IV HCPHW-G 1.3 III IV V HCPHW-F 1.0 IV V Composition A 0.9 IV V Negative Control 0.0 V EXAMPLE 12 Occupational Hand Wash Study (Health care Personnel)--The following embodiment of the present invention was used in this test: Composition C (by weight): 0.40 TCS/- 5DPG/15SXS/0.75ALS/0.75CAPB/0.129 Disodium Phosphate/0.066 Citric Acid, buffer (ph~6)/0. 1 Cetyl Alcohol/0.05 fragrance/1 Sodium PCA/2.97GLY/0.25 Polywaternium- 100/0.1 Aloe Vera Gel/0.00005 FD&C Red #4/0. 000025 Yellow #5.

Composition C was tested vs. commercially available HCPHW-E in an occupational hand wash study. It is expected that a health care worker

would have a greater exposure over an extended time period to a hand wash than the general public.

Accordingly, this test was designed to mimic the population demographics and hand wash patterns likely to be encountered in a health care setting.

HCPHW-E was selected based on its prior acceptance in the health care industry as being an efficacious and mild health care personnel hand wash.

Methodology The study demographics were selected to mimic a population cross section encountered in a health care setting. Thirty-eight volunteers, who were in good general health, participated in the study. The panel included nine volunteers with clinically assessed"dry skin,"and twenty-nine volunteers with clinically assessed"normal skin." These determinations were made by an expert grader following a two-week preconditioning period during which all volunteers washed with a commercially available mild skin care soap bar and discontinued the use of all topically applied moisturizers, creams, lotions, and antibacterial products. Each panelist was qualified for participation after the two-week preconditioning period. The age range of the panelists was between 20 and 55 years of age, and the sex distribution was three males and thirty- five females.

The test compositions were coded and sent to an independent laboratory for testing. The test was a single-blind study in which only the wash monitors were aware of the coded product assignments

when the products were applied to the hand and volar forearm. All wash procedures were conducted in a separate area in order to maintain blinding of the expert grader and instrument operators. The test materials were dispensed by a wash monitor into the hand of the panelists during the wash procedure.

Using appropriate randomization, panelists were assigned a wash partner for"skin-to-skin" friction. Composition C was applied to dry skin and spread over the hand and forearm for 30 seconds.

Immediately thereafter, the panelists were instruc- ted to rinse the hand and forearm for 15 seconds.

The skin was patted dry with a disposable towel.

HCPHW-E was applied to wetted skin and spread over the designated hand and forearm for 30 seconds.

Immediately following, panelists were instructed to rinse the hand and forearm for 15 seconds. The skin was patted dry with a disposable towel. The time between wash cycles was approximately five minutes.

The time between the tenth cycle and grading was approximately twenty minutes. These protocols were chosen to represent typical in-use scenarios en- visioned for both samples used as commercial prod- ucts.

To determine the effects that the two test compositions had on panelists'skin, both visual expert grading and instrumental evaluations were used. Expert grading involved the"Dryness," "Erythema,"and"Tactile Roughness"scales summarized below. Base line expert gradings and instrumental measurements were taken between the start of the first wash cycle on day one. Each panelist was graded, then participated in ten (10)

wash cycles in the morning, graded again, and then subjected to ten (10) wash cycles in the afternoon.

Instrumental measurements were taken at termination of use of a composition, or at completion of the study.

Dryness 0=None 1=Slight flaking or occasional small lifting of scales 2=Moderate flaking/scaling 3=Marked scaling/slight fissuring, cracking, lifting of scales 4=Severe scaling, cracking, and fissuring Erythema 0=None 1=Mild diffuse erythema, limited to a small area 2=Moderate pinkness, more extensive area 3=Marked erythema, may include deeper areas of erythema/slight edema 4=Severe erythema, or presence of edema, fissuring, possible erosions Tactile Roughness 0=Normal 1=Slight roughness 2=Moderate roughness 3=Severe roughness 4=Extreme roughness At the end of the study, the subjects completed a questionnaire directed to their percep- tion of dryness, tightness, itching, and burning for each hand/arm. The scale used for rating was:

Self-assessment No dryness 0 1 2 3 4 severe dryness No tightness 0 1 2 3 4 severe tightness No itching 0 1 2 3 4 severe itching No burning/soreness 0 1 2 3 4 severe burning/soreness Results The tests used in this study are summarized in the following table. Observation of number of indicates panelists'ability panelists able to complete to tolerate composition in test high use situation; more panelists able to complete test=milder product Visual Expert Grading dryness, erythema, roughness; lower reading=milder product Panelist Self-assessment perception of dryness, tightness, itching,, burning/soreness ; lower reading=milder product Minolta Chromameter instrumental reading of skin redness; lower reading=skin less irritated Transepidermal Water Loss instrumental assessment of (TEWL) skin barrier function; lower reading=less damage to skin barrier function- Number of Panelists Able to Complete Test The number of panelists able to complete all washings was significantly greater with Compo- sition C than with HCPHW-E. In addition, the total number of washings completed without significant

redness, dryness, and roughness was higher for Com- position C than for HCPHW-E. Less dryness and red- ness was observed on forearms washed with Composi- tion C than forearms washed with HCPHW-E. These results are illustrated in the graphs of Fig. 1A and Fig. 1B.

Visual Expert Grading Expert Grader Evaluations were performed using a four-point scale on panelist dorsal hands, webbing of fingers, and volar forearms for qualita- tive measurements of dryness, erythema (redness), and tactile roughness. The"Total Panel"consisted of all panelists, i. e., those with normal skin and with dry skin. Less dryness and redness were ob- served on forearms washed with Composition C than those washed with HCPHW-E. For dry skin subjects, the expert grader assessed determined that the panel experienced less redness while using Composition C was used. The results are illustrated in the following two tables.

Expert Grader Assessment of Total Panel Webbing of WEbbing of Fingers Fingers Dorsal Hand Dorsal Hand Volar Forearms Volar Foreamrs Composition C HCPHW-E Composition C HCPHW-E Composition C HCPHW-E Dryness Mean 1.2 1.0 1.2 1.0 1.0 1.1 Standard 0.9 0.7 0.8 0.8 0.7 0.7 Deviation Paired T-Test 0.35 0.34 0.81 Roughness Mean 1.9 1.5 1.7 1.5 1.1 1.1 Standard 0.7 0.7 0.8 0.7 0.4 0.4 Deviation Paired T-Test 0.00 0.01 0.86 Erythema Mean 1.0 1.1 1.6 1.6 2.1 2.8 Standard 0.6 0.7 0.6 0.5 0.9 0.6 Deviation Paired T-Test 0.45 0.45 0.00 Expert Grader Assessment of Panelists with Dry Skin (Termination and EndPoint Scores) Webbing of Webbing of Fingers Fingers Dorsal Hand Dorsal Hand Volar Forearms Volar Foreamrs Composition C HCPHW-E Composition C HCPHW-E Composition C HCPHW-E Dryness Mean 1.2 0.2 1.4 1.5 1.0 1.0 Standard 0.54 0.53 0.77 1.2 1.0 0.74 Deviation Paired T-Test 0.02 0.97 0.95 Roughness Mean 1.9 1.9 2.3 2.3 1.1 1.1 Standard 0.46 0.38 0.65 0.36 0.51 0.49 Deviatiion Paired T-Test 0.86 0.63 1.0 Erythema Mean 1.0 1.1 1.4 2.0 1.9 2.8 Standard 0.46 0.60 .049 0.78 0.98 0.51 Deviation Paired T-Test 0.12 0.10 0.04

Panelist Self-Assessment Panelist perception of the test composi- tions was obtained at the end of the study. The scale used by the subjects to assess the composition as set forth in the methodology section. Panelists were asked to rank their overall impression of the two test composition for four characteristics: dry- ness, tightness, itching, and burning. The percep- tion for the total panel ranked Composition C as being significantly less drying and experiencing significantly less tightness, less itching sensa- tion, and less burning than HCPHW-E. The results are summarized in the following tables.

Self-Assessment at End of Study Dryness Dryness Tightness Tightness Itching Itching Burning Burning Composition C HCPHW-E Composition C HCPHW-E Composition C HCPHW-e Composition C HCPHW-E Mean 2.1 2.6 2.1 2.7 1.3 2.2 1.7 2.9 Std. 1.1 1.3 1.2 1.2 1.2 1.2 1.3 1.4 1.3 Dev. Paired 0.0039 0.0056 0.0002 0.0000 T-test Self-Assessment at End of Study (Dry Skin Panelists Only) Dryness Dryness Tightness Tightness Itching Itching Burning Burning Composition C HCPHW-E Composition C HCPHW-E Composition C HCPHW-E Composition C HCPHW-E Mean 2.3 2.4 2.1 2.3 1.8 2.1 1.8 2.8 Std. 0.7 1.2 1.2 1.4 1.2 1.8 1.4 1.3 Dev. Paired 0.799 0.729 0.594 0.067 T-test

Minolta Chromameter A Minolta Chromameter was used to quantify the change in surface redness of skin exposed to the wash cycles on both the dorsal hand and volar fore- arm surface. Measurements are taken along a red color spectrum, with increasing irritation repre- sented by increasing redness along the color spec- trum. Both the dorsal hand and volar forearm mea- surements were consistent with the Expert Grader assessments. The dorsal hand surface was signifi- cantly less red for sites washed with Composition C than sites washed with HCPHW-E. The volar forearm demonstrated an even greater difference between sites washed with Composition C and HCPHW-E. In particular, Composition C exhibited very minor changes in redness at the sites where measurements were taken. Chromameter values at end-point and termination show that Composition C is significantly less irritating the HCPHW-E. The results are summarized in Fig. 2.

Transepidermal Water Loss (TEWL) Transepidermal Water Loss (TEWL) values for the total panel, at termination of the test, demonstrate that Composition C causes significantly less damage to the skin surface than HCPHW-E.

Normally, the skin surface has barrier functions, both protecting from external influences and pre- serving internal balances. TEWL is a measurement that quantifies the amount of water escaping from the skin surface as a result of damage due to

washing with a surfactant. Composition C produced significantly less damage to the skin surface, when quantified by water loss, than HCPHW-E on both the dorsal hand and volar forearm. The results are summarized in Fig. 3.

Summary Under the conditions used in this example, Composition C is milder than HCPHW-E. Total panel self-assessments reported experiencing less dryness, tightness, itching, and burning when using Composi- tion C. For dry skin subjects, expert grader assessments determined that the panel experienced less redness when using Composition C, and a greater ability to complete more washes when using Composi- tion C. Dry skin panelists in the self-assessments, also reported experiencing less dryness, tightness, itching, and burning when using Composition C. In- strumental assessments for the whole panel signifi- cantly favored Composition C because of imparting significantly less damage to skin functions than HCPHW-E.

The examples show the unexpected benefits achieved by compositions of the present invention.

The data presented above illustrate that a present antibacterial composition can contain ingredients to enhance product esthetics and to impart skin care properties, and can exhibit a log reduction of at least about 2 (after 30 seconds) or at least about 3 (after 60 seconds) vs. S. aureus, or of at least

about 2.5 (after 30 seconds) or at least about 3.5 (after 60 seconds) vs. E. coli.

The antibacterial compositions of the present invention have several practical end uses, including hand cleansers, mouthwashes, surgical scrubs, body splashes, hand sanitizer gels, and similar personal care products. Additional types of compositions include foamed compositions, such as creams, mousses, and the like, and compositions containing organic and inorganic filler materials, such as emulsions, lotions, creams, pastes, and the like. The compositions further can be used as an antibacterial cleanser for hard surfaces, for exam- ple, sinks and countertops in hospitals, food ser- vice areas, and meat processing plants. The present antibacterial compositions can be manufactured as dilute ready-to-use compositions, or as concentrates that are diluted prior to use. The compositions can be applied to a surface, then either rinsed from, wiped from, or allowed to remain on the treated surface.

The compositions also can be incorporated into a web material to provide an antibacterial wiping article. The wiping article can be used to clean and sanitize skin or inanimate surfaces.

The present antimicrobial compositions provide the advantages of a broad spectrum kill of Gram positive and Gram negative bacteria in short contact times. The short contact time for a sub- stantial log reduction of bacteria is important in view of the typical 15 to 60 second time frame used to cleanse and sanitize the skin and inanimate sur- faces.

The present compositions are effective in short contact time because the antibacterial agent is present in the aqueous continuous phase of the composition, as opposed to surfactant micelles. The antibacterial agent, therefore, is available to immediately begin reducing bacterial populations, and further is available to deposit on the skin to provide residual antibacterial efficacy. In addi- tion, because the antibacterial agent is in solution as opposed to surfactant micelles, the absolute amount of antimicrobial agent in the composition can be reduced without adversely affecting efficacy, and the antibacterial agent is not rinsed from the skin with the surfactant prior to performing its antibac- terial function. In addition, the amount of sur- factant in the present antibacterial compositions typically is low, thereby providing additional en- vironmental benefits. Furthermore, the present compositions exhibit excellent esthetic properties, especially with respect to foam generation and foam stability, making the compositions useful in pump foam dispersers.

Obviously, many modifications and varia- tions of the invention as hereinbefore set forth can be made without departing from the spirit and scope thereof, and, therefore, only such limitations should be imposed as are indicated by the appended claims.

APPENDIX A <BR> <BR> SKINCARE AGENTS Acetyl Troctyl Citrate ppncot Kernel M PEG-6 Esters Butyl Acety Ri@@oleate Butyl Mynstate ButyOleate Butyl Stearate C18-36 Acid GlycolEster C12-15 Alcohols Benzoate C12-15 Alcohols Lactate C12-15 AlcoholsOctanoate C15-C18 Glycol C18-20 Glycol Isostearate C14-16 Alycol Palmitate C11-15 Pareth-3 Oleate C11-15 Pareth-3Stearate C11-15 Pareth-12 Stearate C12-15 Pareth-9 Hydrogenated Taitowate C12-15 Pareth-12 Oleate Caprylic/Capric/Diglycerylsucrate Caprylic/Capric Glycendes Caprylic/Capric/Isostearic/Adipic Triglycendes Cetyl Acetate @etylarachidol Cocoqlycendes Com Oi PEG-6 Esters CottonseedGlycende Dibutyl Adipate DibutylSebacate Di-c12-15 Alcohols A@@pate Dicapryl Aclipate NicetyAdipate Diethylene Glycol Dibenzoate Diethyl Palm@toyl Aspartat DiethylSebacate Dihexyl Adipate Dihydrocholesteryl Octyldecancate DihyOropMytosteryt Octyidecanoate Dihydroxyethyl Soyamine Dioleate Dihydroxyethyl Tallowamine O@eate D@sobutylAdipate D@socetylAdipate D@sodecylActuate DiisopropylAclipate DiisopropylD@noleate D@sopropyl Sebacate DipropyleneGlycol Dioenzoate Dit@decyl Adipate Ethyl Arachidonate EthylLaurate Ethyl Linoteate Ethyl Linolenate EthylMorrhuate EthylMynstate Ethyl Palmtate Ethyl Petargonate Ethyl Persate EthylStearate Fish Glycendes GyceryiBenenate Glyceryl Caprate Glyceryl Caprylate Gtyry Caprytate/Caprate GlycerylCocoate Glyceryl Diaurate Glyceryl Dioleate Glycery Distearate Glyceryl Eruate Glyceryl Hydroxystearate Glyceryllsostearate Glyceryl Lanolate Glyceryl Laurate Glyceryl Unoleate GlycerylMynstate Glyceryl Oleate Glyceryl PalmitateLactate GlycerylRicnoleate Glyceryl Sesquoleate GlycerylStearate Glyceryl Stearate Citrate Glyceryl Stearate Lactate Glyceryl Tnacetyl Hydroxystearate Glyceryl Triacetyl Ricinoleate GlycerylTrioctanoate Glyceryl Triundecanoate Glycol Dioctanoate GlycolHydroxystearate Glycol Oleate Glycol Ricinoleate Glycol Stearate Heptylundecanol Hexyl Laurate Hydrogenated Coco-Glycerides Hydrogenated Lard Glycende Hydrogenated Lard Glycendes Hydrogenated Palm Glycendes Hyorogenated Palm Kernel Glycendes Hyarogenatecf Palm Oil Glycende Hydrogenated Palm Oil Glycendes Hyarogenateo Palm/Palm Kernel Oil PEG-6 Esters Hydrogenated Polyisobutene HydrogenatedSoybean Oil Glycendes Hyarogenated Soy Glycende HydrogenatedTallow Glycence Hydrogenated Tallow Glycerine Citrate Hydrogenated Tallow Glycerine Lactate Hydrogenated Tallow Glycenoes Hydrogenated Tallow Glycendes Citrate Hydrogenated Vegetable Glyceride Hydrogenated Vegetable Glycerides Hydrogenated Vegetable Glycerides Phospnate HydrogenatedLanolin Hydroxyoctacosanyl Hydroxystearate Isoamy Laurate isobutyl Mynstate IsobutylPatate IsoDuty1 Pelargonate Isobutyl Stearate isohexyl Laurate IsohexyPalmtate Isopropyl Isostearate IsopropylLanotate Isopropy Laurate isopropyl Linoleate <BR> <BR> tsopropy ! Methoxyannamate IsopropylMynstate Isopropyl Oleate Isopropyl Palmitate Isopropyl Ricnoleate Isopropyl Stearate IsopropylTallowate IsostearyAlconol Isostearyl Benzoate IsostearylIsostearate Isostearyl Lactate IsostearylNeopentanoate IsostearylPatmtate Isotndecyl Isononanoate Aneth-9Acetate Laneth-10Acetate Lanohn Lard Glycendes Laureth-2Benzoate laityisostearate LaurylLactate Methy Acetyl Ricinoleate Methyt Caproate MethylCaprytate Methyl Caprylate/Caprate MethylCocoate MethylDehydroabietate Methyl Glucose Sesquioleate Methyl Glucose Sesquistearate Methyl Hydrogenated Rosinate Methyl Hydroxystearate Methyl Laurate Methyl Linoleate MethylMynstate Methyl Oleate MethylPalmitate MethylPelargonate MethylRicnoleate MethylRosnate MethylStearate Myreth-3 Caprate Myreth-3 Laurate Myreth-3Myristate Myreth-3Palmitat Neopentyl Glycol Dicaprate Neopentyl Glycol Dictanoate NonylAcetate OctylAcetoxystearate iOctyldodecylNeodecanoate octalHydroxystearate Octyl Isononanoate Palm Kemei Glycendes Palm Oil Glycendes PEG-6 Caprylic/Capric Glycendes PEG-2 Castor oa PEG-3 Castor Oi PEG-4 Castor Oil PEG-5 Castor Oil PEG-8 Castor Oil PEG-9 Castor Oil PEG-10 Castor Oil PEG-10 Coconut Oil Esters PEG-5Glyceryl Trisostearate PEG-5 Hydrogenated Castor Oil PEG-7 Hydrogenated Castor Oil PEG-5 Hydrogenated Com Gtyeenaes PEGS Hydrogenated Fish Glycendes PEG-20 Metthyl Glucose Sesqustearate Pentaerytnnty Roseate PentaerythntylTetraoctanoate Pentaerythntyl Tetraoleate PPG-4-Ceteth-1 PPG-8-Ceteth-1 PPG-4-Ceteth-2 PPG-10 Cetyl Ether PPG10 Cetyl Ether Phosphate PPG-28 Cetyl Ether PPG-30 Cetyl Ether PPG-50 Cetyl Ether PPG-17 Dioleate PPG-3 Hyorogenatec Castor Ou PPG-30 Isocetyl Ether PPG-5Lanoiate PPG-2 Lanolin Alcohol Ether PPG-5 Lanolin Alcohol Ether PPG-10 Lanolin Alcohol Ether PPG-20 Lanolin Alcohol Ether PPG-30 Lanoin Alcohol Ether PPG-5 Lanolin Wax PPG-5 Lanolin Wax Glycende PPG-9 Laurate PPG4 Lauryl Ether PPG-3 Mynstyl Ether PPG-4 Mynstyl Ether PPG-26 Oieate PPG-36 Oleate PPG-10 Oleyl Ether PPG-20 Oleoyl Ether PPG-23 Oleoyl Ether PPG-30 Oleat Ether PPG-37 Oleat Ether PPG-50Oleyl Ether PPG-9-Steareth-3 PPG-11 StearylEther PPG-15Stearyl Ether Propène Glycol Isostearate Propylene Glycol Hyaroxystearate Propylene GlycolLaurate Propylene Glycol Mynstate PropyleneGlycol Mynstyl Ether Propylene Glycol Mynstyl Ether Acetate Propylene Glycol Oleate Propylene Glycol Ricinoleate Propylene GlycolSoyate Propylene Glycol Stearate SoySterol Soy Sterol Acetate Soualene Stearoxytnmethylsitane SucroseDtstearate Su@@unzed Jojoba Oil Sunflower Seed Oil Glycendes Tail Oil Glycendes TalowGlycende TallowGlycendes TriisocetylCitrate Trilsosteann PEG-6 Esters Trimethylsilylamodimeth@cone Triolen PEG-6 Esters Tris(Tributoxysiloxy)Methylsilane Vegetate Glycenodes Phospnate Wheat Germ Glycendes Adenos@@e Phosphate <BR> <BR> Adenos@@eTnpnosphate<BR> <BR> <BR> <BR> Alarme Aidioxa <BR> <BR> <BR> Allantoin<BR> <BR> <BR> <BR> A@@anto@@Ascorbate<BR> <BR> <BR> <BR> Alantonbots) Atantoin Calcium Pantothenate AlantonGalacturonic Acid Aliantoin Glycyrrhetinic Acid Alantoin Polygaiacturomc Acid Aloe Animal Collagen Amino Aads Animal Elastin Amino AaUs Animal Keratin Amno Acids Arginine Asparagne Aspartic Acid Camphor Caprylyl/Capryl Gucoside casas CetylBetaine Chlorodeceth-14 Cholesterol Cocamidopropyl Lauryl Ether Cysteine CysteneHa Cystine Desamido Ainmal Collagen DicapryloylCystine Diethyl Aspartate MethyleneTricaseinamide DiethylGlutamate Dihydrocholesterol Dipamitoyl Hydroxyproline Disodium Aaenosne Tnphosphate Ethyl Aspartate Ethyl Ester of Hydrolyzed Animal Protein EthylGlutamate EthylSennate EthylUrocanate Fois rad Fructose Glutamic Acid Outamtne Glyceryl Lanolate Glycine Gtycogen Guanosne Hexamethyldisiloxane HexyNicotinate Histcine Human Placenta Protein Hyaturonc Aad Hydrogenated Animal glycende <BR> <BR> HyorogenatedLaneth-5<BR> <BR> <BR> <BR> HyorotyzedAnimal Elastn Hydrolyzed Animal Keratin HycfotyzM Animal Protein Hydrolyzed Casein HyOrotyzeO Human Placental Protein Hydrolyzed Mucopolysacchandes HydrolyzedSik Hydrolyzed Soy Protein Hydrolyzed Vegetable Protein Hydrolyzed Yeast Proton HydrolyzedLanolin Hydroxyproline isoleucine Keratin Laneth-4 Phosphate Laneth-5 Lanoinamoe DEA Lanosteroi LarOGlycenaes Lauramidopropyl Be@aine LaurylAminoprop@iglycine <BR> <BR> Lauryl Diethyleneckam@oglyc@@e<BR> <BR> <BR> <BR> Lecith@@<BR> <BR> <BR> <BR> Leuc@@e Lysne Magnesum Aspartate Magnsium Lanolate MEA-Hydrolyzed Animal Protein Methionine <BR> <BR> 2-Methyl-4-Hydroxypyrrolidine<BR> <BR> @<BR> <BR> <BR> <BR> <BR> M@xed Isopropanolamines Lanolate MixedMucopolysacchandes Monosaccnaride Lactate Convens Niaonamiae Norvaline Otey) Betane @rotic Acid Palmtoyl Animal Collagen Amino Acide PEG Hydrogenated Lanolin PEG-10 Hyarogenatea Lanolin PEG-2M@@x Solids PEG-6 Soya Sterol Undecylenate Phenylalanine Polyglyceryl-2 Lanolin Alcohol Ether Potassium Aspartate PotassiumCasemate Potassium DNA PPG-2-Buteth-3 PPG-3-Buteth-5 PPG-5-Buteth-7 PPG-7-Buteth-10 PPG-9-Buteth-12 PPG-12-Buteth-16 PPG-15-Buteth-20 PPG-20-Buteth-30 PPG-24-Buteth-27 PPG-26-Buteth-26 PPG-28-Buteth-35 PPG-33-Buteth-45 PPG-4ButylEther PPG-5 Buty) Ether PPG-9 Butyl Ether PPG-14Butyl Ether PPG-15 Butyl Ether PPG-16 Butyl Ether PPG-18 Butyl Ether PPG-22Buty ! Ether PPG-24 Butyl Ether PPG-30 Butyl Ether PPG-33 Butyl Ether PPG-40 ButA Ether PPG-53 Butyl Ether PPG-2Isostearate PPG-10 Methyl Glucose Ether PPG-20 Methyl Glucose Ether PPG-20 Methyl Glucose Ether Acetat PPG-2 Mynstyl Ether Propionate Pregnenolone Acetate <BR> <BR> Pro@ne<BR> <BR> <BR> <BR> Pyridoxine Pyndoxine Dicaprytate Pyndoxine Dilaurate Pyndoxine Dioctenoate PyndoxneDipatmftate Pyridoxine HCl PyridoxineTripalmitate ResorrndAcetate Retint Retinal Acetate Retinyl Palmitate RibonudeicAcza Ricin oleamidopropyl Betaine SalicylicAcid seme Serum Albumin SerumProteins Silk Amino Acids SodiumCasenate Sodium Chondrortin Sulfate Sodium DNA SodiumGtuconate SodiumGlutamate Sodium Hyaluronate Sodium Lactate Methylsiland Sodium Laneth Sonate Sodium Mannuronate Methylsiland Sodium PCA Methyslianol SodiumRiboflavin Phosphate Sodium Urocanate Soluble Animal Collagen Sorortol Soyaethyl Morpholinium Ethosutfate Soy Protein Sulfurized Jojoba Oil Tat 01 Sterol ThamineHCI Theme Nitrate Threonine TocopherolAcetate Tocopheryl Linoleate TocopherolNicotinate TocopherylSuccinate TridecylSaticytate Tndecyl Stearate Tryptophan Tyrosine unc Acid UrocanicACid Wheat Germamidopropyl Dmettiytamne Lactate Whey Protein Octylacrylamide/Acrylates/Butylaminoethyl Metnaaytate Copolymer Octylacrylamide/Acrylates Copolymer PEG-22/Dodecyl Glycol Copolymer PEG-45/DodecylGlycol Copolymer PEI-7 <BR> <BR> PEI-30<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> PEI-30<BR> PEI-45 PEI-275 PEI-700 PEI-1000 PEI-1500 PEI-2500 Polyacrylamkide Polyacrylamidomethylpropane Sutfonic AOQ PolyacrylicAaa Polyaminopyropyl Biguanide Polyamno Sugar Condensate Polyquatemium-1 Polyquatemium-2 Polyquatemium-4 Polyquatemium-5 Polyquatemium-6 Polyquatemium-7 Polyquatemium-8 Polyquatemium-9 Polyquatemium-10 Polyquatemium-11 Polyquatemium-12 Polyquatemium-13 Polyquatemium-14 Polyquatemium-15 Potyvinyl Alcohol Potywiyt Butyrat PolyvinylImidazo@nium Acetate PolyvinylLaurate Polyvinyl Methyl Ether PVM/MA Copolymer PVP PVP/Dimethylaminoethylmethacrylate Copolymer PVP/Ethyl Methacrylate/Methacrylic AcidCopolymer PVP/HexadeceneCopolymer Sodium Polystyrene Sulfonate Sodium Styrene/Acrylate/PEG-10 Dimaleate Copolymer Starch/Acrylates/Acrylamide Copolymer Stearytvinyl Ether/Maleic Anhydride Copolymer Styrene/PVP Copolymer Sucrose Benzoate/Sucrose Acetate Isobu- tyrate/Butyl Benzyl Phthalate Copolymer Sucrose Benzoate/Sucrose Acetate Isobu- tyrate/Butyl Benzyl Phthalate/Methyl MettcrytateCopolymer Sucrose Benzonate/Sucrose Acetate Isobutyrate Copolymer Meroxapol 105 Meroxapol 108 Meroxapol171 Meroxapol 172 Meroxapol174 Meroxapol178 Meroxaoo)251 Meroxapol 252 Meroxapol254 Meroxapol255 Meroxapol258 Meroxapol311 <BR> <BR> Meroxapol 312<BR> <BR> <BR> <BR> Meroxapol 314 PEG-4 PEG-6 <BR> <BR> PEG-A<BR> <BR> <BR> PEG-9 PEG-10 <BR> <BR> PEG 12<BR> <BR> <BR> <BR> PEG-14 PEG-16 PEG-18 PEG-20 PEG-32 PEG-40 PEG-6-32 PEG-75 PEG-135 PEG-150 PEG-200 PEG-350 <BR> <BR> <BR> <BR> <BR> <BR> PEG-2M<BR> <BR> <BR> PEG-5M<BR> PEG-7M PEG-9M PEG- PEG-20M PEG-23M PEG-45M PEG- PEG-115M PEG/PPG-17/6 Copolymer PEG/PPG-18/4Copolymer PEG/PPG-23/50Copolymer PEG/PPG-35/9 Copolymer PEG/PPG-125/30Copolymer Poloxamer101 Poloxamer 105 Poloxamer 108 Poloxamer 122 Poloxamer 123 Potoxamer 124 Poloxamer 181 Poloxamer 182 <BR> <BR> Poloxamer 183<BR> Poloxamer 184 Poloxamer 185 Poloxamer 188 Poloxdamer 212 Poloxamer 215 Poloxamer 217 Poloxamer 231 Po)234 <BR> <BR> Poloxamer 235<BR> <BR> <BR> <BR> Poloxamer 237 Poloxamer238 Poloxamer282 Poloxamer 284 Poloxamer288 Poloxamer 331 Poloxamer333 Poloxamer 334 Poloxamer 335 Poloxamer 338 Poloxamer 401 Poloxamer 402 Poloxamer 403 Poloxamer 407 PPG-9 PPG-12 PPG-15 PPG-17 PPG-20 <BR> <BR> PPG-26<BR> PPG-30 PPG-34 <BR> <BR> Acacia<BR> Agar<BR> <BR> <BR> Algin Alginic Acid AmmoniumAlginate Calcium Alguate Calcium Carrageenan c Gum<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> Demer<BR> Dextran Dextrn Carboxymethyl Hydroxyethylcelulose CarooxymetnylHyoroxypropyt Guar Carrageenan Ethylce@@ulose Gelatin Guar Gum GuarHydroxypropyttnmonium Chloride Gum BenzOin Hydroxybutyl Methylcellulose Hydroxyethylcellulose Hydroxyethyl Ethylcellulose Hydroxypropylcellulose Hydroxyprop)Guar Hydroxypropyl Methylcellulose . Resin Karaya Gum Kelp Locust Bean Gum Maltodextnn Methylcellulose Otanum Pectin Potassiumagnate PotassiumCarrageenan Propylene Glycol Alginate 8andarac Gum SodiumCarboxymethyl Dextran Sodium Carrageenan SodiumCellulose Sulfate Tragacanth Gum Xanthan Gum Acrylamides Copolymer Acrylamide/Sodium Acrytate Copolhymer Acrytate/Acrylamide Copolymer Acrylate/Ammonium Methaaylate Copolymer AcrYlatesCOpolymer Acrylates/DiacetoneacrylamideCopolymer Acrytates/Steareth-20 Methacrylate Copolymer Acrylic/Acrylate Copolymer Adipic Acid/Dimethylaminohydroxypropyl Diethylenetnamine Copolymer Adipic Acid/Epoxypropyl Diethylenetnamine Copolymer Aryl Stearate Copolymer AminoethylacrylatePhosphate/Acrytate Copolymer Ammonium Acrylates Copolymer Ammonium Styrene/Acrytate Copolymer AmmoniumVinyl Acetate/Acrylates Copolymer AMP Acrylates/Diacetoneacrylaminde Copolymer AMPDAcrytates/Diacetoneacryamide Copolymer BenzoeAcid/Phthalic Anhydride/Pentaerythri- tol/Neopentyl Glycol/Palmitic Acid Copolymer Carbomer 910 Carbomer 934 Carbomer 934P Carbomer 940 Carbomer 941.

Corn Starch/Acrylamide/Sodium Acrylate Copotymer DEA-Styrene/Acrylates/Divinylbenzene Copolymer Diemyene Glycolamine/Epichlorohydrin/ PiperazineCopolymer Dodecanedioic Acid/Cetearyl Alcohol/Glycol Copolymer Ethylene/Acrylate Copolymer HydroxyethylPEI-1 000 HydroxyethylPEl-1500 Isobutylene/Maleic Anhydride Copolymer isopropy Ester of PVM/MA Copolymer Methacryloyl Ethyl Betaine/Methacrylates CoPolymer Methoxy PEG-22/Dodecyl Glycol Copolymer