Title:
ANTIBACTERIAL SUSCEPTIBILITY TESTING COMPOSITION COMPRISING MICROBIAL NUCLEASE-DETECTING AGENT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/004807
Kind Code:
A1
Abstract:
The present invention relates to an antibacterial susceptibility testing composition comprising a microbial nuclease-detecting agent and a use thereof. The microbial nuclease-detecting agent is a FRET-DNA probe. When antibacterial susceptibility testing for a specific microbe is conducted with the agent according to the FRET-DNA probe-based AST (F-AST) method, bacterial cells are lysed and total proteins including nucleases are released from the lysate as a result of antibiotic-induced bacterial lysis. In this condition, the F-AST probe is cleaved by the nucleases to separate the quencher distant from the fluorophore, with the consequent significant increase of fluorescence signals, whereby the antibacterial susceptibility of microbes can be investigated accurately, rapidly, and easily. Therefore, the F-AST system of the present invention can be easily conducted and is fast and economically advantageous. Furthermore, being designed to be suitable for reading on a microtitration plate reader, the system can readily utilize conventional techniques and provides a basis for clinical therapy.
Inventors:
PARK KYOUNG SOOK (KR)
YI SO YEON (KR)
LEE UI JIN (KR)
KWON JUNG SUN (KR)
SHIN YONG BEOM (KR)
YI SO YEON (KR)
LEE UI JIN (KR)
KWON JUNG SUN (KR)
SHIN YONG BEOM (KR)
Application Number:
PCT/KR2019/005319
Publication Date:
January 02, 2020
Filing Date:
May 03, 2019
Export Citation:
Assignee:
BIONANO HEALTH GUARD RES CENTER (KR)
International Classes:
C12N15/115; C12Q1/44; G01N33/68
Domestic Patent References:
| WO2013033436A1 | 2013-03-07 |
Foreign References:
| KR20160120681A | 2016-10-18 |
Other References:
LEE, K. J. ET AL.: "Simple and rapid detection of bacteria using a nuclease-responsive DNA probe", ANALYST., vol. 143, 2018, pages 332 - 338, XP055668414
GERASIMOVA, Y. V. ET AL.: "Enzyme-assisted target recycling (EATR) for nucleic acid detection", CHEMICAL SOCIETY REVIEWS, vol. 43, 2014, pages 6405 - 6438, XP055324226, DOI: 10.1039/C4CS00083H
ERDEM, S. S. ET AL.: "Rapid, low-cost fluorescent assay of beta-lactamase-derived antibiotic resistance and related antibiotic susceptibility", JOURNAL OF BIOMEDICAL OPTICS, vol. 19, no. 10, October 2014 (2014-10-01), pages 1 - 11, XP060047113, DOI: 10.1117/1.JBO.19.10.105007
PARK, K. ET AL.: "FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis", BIOSENSORS AND BIOELECTRONICS, vol. 130, 2019, pages 225 - 229, XP055668416
GERASIMOVA, Y. V. ET AL.: "Enzyme-assisted target recycling (EATR) for nucleic acid detection", CHEMICAL SOCIETY REVIEWS, vol. 43, 2014, pages 6405 - 6438, XP055324226, DOI: 10.1039/C4CS00083H
ERDEM, S. S. ET AL.: "Rapid, low-cost fluorescent assay of beta-lactamase-derived antibiotic resistance and related antibiotic susceptibility", JOURNAL OF BIOMEDICAL OPTICS, vol. 19, no. 10, October 2014 (2014-10-01), pages 1 - 11, XP060047113, DOI: 10.1117/1.JBO.19.10.105007
PARK, K. ET AL.: "FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis", BIOSENSORS AND BIOELECTRONICS, vol. 130, 2019, pages 225 - 229, XP055668416
Attorney, Agent or Firm:
KIM, Soon Woong (KR)
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