Title:
ANTIBODIES SPECIFIC FOR HYPERPHOSPHORYLATED TAU AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/009308
Kind Code:
A3
Abstract:
The present invention relates to a novel class of monoclonal antibody that specifically binds the phosphorylated serine 396 residue on pathological hyperphosphorylated (PHF) tau (pS396), as well as to methods of using these molecules and their tau binding fragments in the treatment of Alzheimer's disease and tauopathies.
Inventors:
PEDERSEN JAN TORLEIF (DK)
PEDERSEN LARS ØSTERGAARD (DK)
DÄCHSEL JUSTUS CLAUS ALFRED (DE)
ABDUR-RASHEED ASUNI AYODEJI (DK)
ROSENQVIST NINA (SE)
PEDERSEN LARS ØSTERGAARD (DK)
DÄCHSEL JUSTUS CLAUS ALFRED (DE)
ABDUR-RASHEED ASUNI AYODEJI (DK)
ROSENQVIST NINA (SE)
Application Number:
PCT/EP2016/066470
Publication Date:
April 06, 2017
Filing Date:
July 12, 2016
Export Citation:
Assignee:
H LUNDBECK AS (DK)
International Classes:
A61K39/395; A61K49/00; A61P25/28; C07K16/18; G01N33/68; A61K39/00
Domestic Patent References:
| WO2012045882A2 | 2012-04-12 | |||
| WO2013050567A1 | 2013-04-11 | |||
| WO2002027017A2 | 2002-04-04 |
Other References:
ANONYMOUS: "Mouse anti-Phospho-Tau 396 Mouse anti-Phospho-Tau 396; Catalog No. 35-5300", INVITROGEN CATAOLOGUE CATALOG NO. 35-5300, 1 October 2008 (2008-10-01), XP055308498, Retrieved from the Internet [retrieved on 20161007]
ANONYMOUS: "Product datasheet Anti-Tau (phospho S396) antibody [EPR2731] ab109390", ABCAM PRODUCT CATALOGUE, 1 January 2014 (2014-01-01), XP055308426, Retrieved from the Internet [retrieved on 20161006]
JOËLLE ROSSEELS ET AL: "Tau Monoclonal Antibody Generation Based on Humanized Yeast Models", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 290, no. 7, 24 December 2014 (2014-12-24), US, pages 4059 - 4074, XP055250974, ISSN: 0021-9258, DOI: 10.1074/jbc.M114.627919
DAVID SINGER ET AL: "Characterization of Phosphorylation Dependent Antibodies To Study the Phosphorylation Status of the Tau Protein", INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS ; FORMERLY KNOWN AS LETTERS IN PEPTIDE SCIENCE, KLUWER ACADEMIC PUBLISHERS, DO, vol. 11, no. 4, 1 December 2005 (2005-12-01), pages 279 - 289, XP019287635, ISSN: 1573-3904
ANONYMOUS: "Application Abreviews Notes", 26 March 2014 (2014-03-26), XP055342014, Retrieved from the Internet [retrieved on 20170203]
"Tau [pS199] ABfinity (TM) Recombinant Rabbit Monoclonal Antibody - Purified; Cat.No. 701054", 15 September 2011, NOVEX, article ANONYMOUS: "Tau [pS199] ABfinity (TM) Recombinant Rabbit Monoclonal Antibody - Purified; Cat.No. 701054", pages: 1 - 2, XP055342152
"Anti-phospho-Tau (pSer199/202); Data sheet; Cat.No. T6819", 1 January 2006, SIGMA, article ANNYMOUS: "Anti-phospho-Tau (pSer199/202); Data sheet; Cat.No. T6819", XP055342158
"Tau Phosphorylation Site-Specific Antibody Sampler (Containing Tau pS199, pT205, pT231, pS262, pS356, pS396, pS404, pS409, pS422 Rabbit Polyclonal & Tau [TAU-5] Monoclonal Antibodies, Unconjugated) PRODUCT ANALYSIS SHEET", 1 January 2010, INVITROGEN, article ANONYMOUS: "Tau Phosphorylation Site-Specific Antibody Sampler (Containing Tau pS199, pT205, pT231, pS262, pS356, pS396, pS404, pS409, pS422 Rabbit Polyclonal & Tau [TAU-5] Monoclonal Antibodies, Unconjugated) PRODUCT ANALYSIS SHEET", XP055342248
MICHAEL A. BRISTER ET AL: "OGlcNAcylation and Phosphorylation Have Opposing Structural Effects in tau: Phosphothreonine Induces Particular Conformational Order", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, no. 10, 12 March 2014 (2014-03-12), US, pages 3803 - 3816, XP055308062, ISSN: 0002-7863, DOI: 10.1021/ja407156m
RUDIKOFF S ET AL: "Single amino acid substitution altering antigen-binding specificity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 79, 1 March 1982 (1982-03-01), pages 1979 - 1983, XP007901436, ISSN: 0027-8424, DOI: 10.1073/PNAS.79.6.1979
YEE WAH WONG ET AL: "Structural Requirements for a Specificity Switch and for Maintenance of Affinity Using Mutational Analysis of a Phage-Displayed Anti-Arsonate Antibody of Fab Heavy Chain First Complementarity-Determining Region", THE JOURNAL OF IMMUNOLOGY, THE AMERICAN ASSOCIATION OF IMMUNOLOGISTS, US, vol. 160, 1 January 1998 (1998-01-01), pages 5990 - 5997, XP007916801, ISSN: 0022-1767
JEFFEREY R JACKSON: "In Vitro Antibody Maturation Improvement of a High Affinity, Neutralizing Antibody Against IL-1 beta", THE JOURNAL OF IMMUNOLOGY, vol. 154, 1 January 1995 (1995-01-01), pages 3310 - 3319, XP055033979
ANONYMOUS: "Product datasheet Anti-Tau (phospho S396) antibody [EPR2731] ab109390", ABCAM PRODUCT CATALOGUE, 1 January 2014 (2014-01-01), XP055308426, Retrieved from the Internet
JOËLLE ROSSEELS ET AL: "Tau Monoclonal Antibody Generation Based on Humanized Yeast Models", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 290, no. 7, 24 December 2014 (2014-12-24), US, pages 4059 - 4074, XP055250974, ISSN: 0021-9258, DOI: 10.1074/jbc.M114.627919
DAVID SINGER ET AL: "Characterization of Phosphorylation Dependent Antibodies To Study the Phosphorylation Status of the Tau Protein", INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS ; FORMERLY KNOWN AS LETTERS IN PEPTIDE SCIENCE, KLUWER ACADEMIC PUBLISHERS, DO, vol. 11, no. 4, 1 December 2005 (2005-12-01), pages 279 - 289, XP019287635, ISSN: 1573-3904
ANONYMOUS: "Application Abreviews Notes", 26 March 2014 (2014-03-26), XP055342014, Retrieved from the Internet
"Tau [pS199] ABfinity (TM) Recombinant Rabbit Monoclonal Antibody - Purified; Cat.No. 701054", 15 September 2011, NOVEX, article ANONYMOUS: "Tau [pS199] ABfinity (TM) Recombinant Rabbit Monoclonal Antibody - Purified; Cat.No. 701054", pages: 1 - 2, XP055342152
"Anti-phospho-Tau (pSer199/202); Data sheet; Cat.No. T6819", 1 January 2006, SIGMA, article ANNYMOUS: "Anti-phospho-Tau (pSer199/202); Data sheet; Cat.No. T6819", XP055342158
"Tau Phosphorylation Site-Specific Antibody Sampler (Containing Tau pS199, pT205, pT231, pS262, pS356, pS396, pS404, pS409, pS422 Rabbit Polyclonal & Tau [TAU-5] Monoclonal Antibodies, Unconjugated) PRODUCT ANALYSIS SHEET", 1 January 2010, INVITROGEN, article ANONYMOUS: "Tau Phosphorylation Site-Specific Antibody Sampler (Containing Tau pS199, pT205, pT231, pS262, pS356, pS396, pS404, pS409, pS422 Rabbit Polyclonal & Tau [TAU-5] Monoclonal Antibodies, Unconjugated) PRODUCT ANALYSIS SHEET", XP055342248
MICHAEL A. BRISTER ET AL: "OGlcNAcylation and Phosphorylation Have Opposing Structural Effects in tau: Phosphothreonine Induces Particular Conformational Order", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, no. 10, 12 March 2014 (2014-03-12), US, pages 3803 - 3816, XP055308062, ISSN: 0002-7863, DOI: 10.1021/ja407156m
RUDIKOFF S ET AL: "Single amino acid substitution altering antigen-binding specificity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 79, 1 March 1982 (1982-03-01), pages 1979 - 1983, XP007901436, ISSN: 0027-8424, DOI: 10.1073/PNAS.79.6.1979
YEE WAH WONG ET AL: "Structural Requirements for a Specificity Switch and for Maintenance of Affinity Using Mutational Analysis of a Phage-Displayed Anti-Arsonate Antibody of Fab Heavy Chain First Complementarity-Determining Region", THE JOURNAL OF IMMUNOLOGY, THE AMERICAN ASSOCIATION OF IMMUNOLOGISTS, US, vol. 160, 1 January 1998 (1998-01-01), pages 5990 - 5997, XP007916801, ISSN: 0022-1767
JEFFEREY R JACKSON: "In Vitro Antibody Maturation Improvement of a High Affinity, Neutralizing Antibody Against IL-1 beta", THE JOURNAL OF IMMUNOLOGY, vol. 154, 1 January 1995 (1995-01-01), pages 3310 - 3319, XP055033979
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