Title:
ANTIBODIES TO NATURAL KILLER CELL AND NON-SPECIFIC CYTOTOXIC CELL RECEPTOR
Document Type and Number:
WIPO Patent Application WO1989003396
Kind Code:
A3
Abstract:
The present invention encompasses a receptor molecule present on the surface of natural killer cells (NK cells) and non-specific cytotoxic cells (NCC) which is involved in non-specific lysis of target cells bearing an antigen recognized by the receptor molecule, an antigen common to the surface of cells recognized and lysed by natural killer cells, monoclonal and heterologous antibodies which bind to the receptor and target cell antigen(s) which are useful in their identification and purification, and methods for altering NK cell-mediated lysis of target cells. The monoclonal antibodies (mAbs) of the present invention were prepared by cell fusions between spleen cells from mice immunized with either non-specific cytotoxic cells (NCC) from catfish (anti-receptor antibodies) or NC-37 human lymphoblastoid B-cells, which are susceptible to lysis by NK cells, and myeloma cells. Eight distinct mAbs have been characterized, four directed against the NK/NCC target cell receptor and four against the NK/NC cell antigen. As used in the present invention "NK cells" will be used to include both natural killer cells of mammalian origin and non-specific cytotoxic cells of fish origin, unless specifically stated otherwise. Two of the mAbs directed against the receptor and two of the mAbs against the NK target cell antigen are able to significantly inhibit NK lysis at low mAb concentrations, as assessed by inhibition of cellular toxicity. Two of the mAbs directed against the receptor and two of the mAbs against the NK target cell antigen bind to the antigen(s) but do not inhibit lysis.
Inventors:
EVANS DONALD L (US)
Application Number:
PCT/US1988/003606
Publication Date:
June 01, 1989
Filing Date:
October 14, 1988
Export Citation:
Assignee:
UNIV GEORGIA RES FOUND (US)
International Classes:
A01K49/00; A61K39/395; C07K1/22; C07K14/705; C07K16/00; C07K16/28; C12N5/10; C07K14/435; C12N15/02; C12P21/08; G01N33/53; G01N33/566; G01N33/577; A61K38/00; C12R1/91; (IPC1-7): C07K15/06; C12N5/00; C12N15/00; C12P21/00; A61K39/395; G01N33/50
Foreign References:
| EP0160486A2 | 1985-11-06 |
Other References:
Biological Abstracts/RRM, volume 34, no. 73001, 1988, D. Harris et al.: "Identification of a putative human natural killer NK cell antigen receptor", see title and terms & Leukocyte Biol. 1987. vol. 42, no. 4, p. 368
Biological Abstracts/RRM, volume 34, no. 117603, 1988, D. Evans et al.: "Characterization of the antigen receptor on fish non-specific cytotoxic cells", see title and terms & 72nd Annual Meeting of the Federation of American Societies for Experimental Biology, Las Vegas, Nevada, USA, May 1-5, 1988
Biological Abstracts/RRM, volume 35, no. 28038, 1988, L. Friedmann et al.: "Identification of a rat NK cell receptor using monoclonal antibodies", see title and terms & FASEB (Fed. Am. Soc. Exp. Biol.) J. 1988, vol. 2, no. 6, p.
Chemical Abstracts, volume 104, no. 23, 9 June 1986, (Columbus, Ohio, US), A. Ythier et al.: "Generation of monoclonal antibodies blocking cytotoxic reactions by human NK clones: further characterization of a 40/80-kDa target cell receptor", see page 563
Chemical Abstracts, volume 103, no. 21, 25 November 1985, (Columbus, Ohio, US), F. Malavasi et al.: "Murine monoclonal antibodies as probes for the phenotypical, functional, and molecular analysis of a discrete peripheral blood lymphocyte population exerting natural killer activity in vitro", see page 549
Biological Abstracts/RRM, volume 34, no. 117603, 1988, D. Evans et al.: "Characterization of the antigen receptor on fish non-specific cytotoxic cells", see title and terms & 72nd Annual Meeting of the Federation of American Societies for Experimental Biology, Las Vegas, Nevada, USA, May 1-5, 1988
Biological Abstracts/RRM, volume 35, no. 28038, 1988, L. Friedmann et al.: "Identification of a rat NK cell receptor using monoclonal antibodies", see title and terms & FASEB (Fed. Am. Soc. Exp. Biol.) J. 1988, vol. 2, no. 6, p.
Chemical Abstracts, volume 104, no. 23, 9 June 1986, (Columbus, Ohio, US), A. Ythier et al.: "Generation of monoclonal antibodies blocking cytotoxic reactions by human NK clones: further characterization of a 40/80-kDa target cell receptor", see page 563
Chemical Abstracts, volume 103, no. 21, 25 November 1985, (Columbus, Ohio, US), F. Malavasi et al.: "Murine monoclonal antibodies as probes for the phenotypical, functional, and molecular analysis of a discrete peripheral blood lymphocyte population exerting natural killer activity in vitro", see page 549
Download PDF: