Title:
ANTIBODY AGAINST HUMAN PROSTAGLANDIN E2 RECEPTOR EP4
Document Type and Number:
WIPO Patent Application WO/2012/043634
Kind Code:
A1
Abstract:
Provided is an antibody, which binds to the subtype EP4 of human PGE2 receptor and inhibits the function of EP4, or a functional fragment thereof. Also provided is a drug comprising said antibody or a functional fragment thereof. Mice were immunized with the subtype EP4 of human PGE2 receptor and a monoclonal antibody suppressing an increase in intracellular cAMP due to EP4 was screened. The CDR sequence of the monoclonal antibody thus obtained was determined.
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Inventors:
TAKAYAMA KIYOSHI (JP)
SHIMIZU TOMOKO (JP)
URUSHIBATA YUJI (JP)
SUGIMOTO YUKIHIKO (JP)
SHIMIZU TOMOKO (JP)
URUSHIBATA YUJI (JP)
SUGIMOTO YUKIHIKO (JP)
Application Number:
PCT/JP2011/072190
Publication Date:
April 05, 2012
Filing Date:
September 28, 2011
Export Citation:
Assignee:
NB HEALTH LAB CO LTD (JP)
UNIV KUMAMOTO NAT UNIV CORP (JP)
TAKAYAMA KIYOSHI (JP)
SHIMIZU TOMOKO (JP)
URUSHIBATA YUJI (JP)
SUGIMOTO YUKIHIKO (JP)
UNIV KUMAMOTO NAT UNIV CORP (JP)
TAKAYAMA KIYOSHI (JP)
SHIMIZU TOMOKO (JP)
URUSHIBATA YUJI (JP)
SUGIMOTO YUKIHIKO (JP)
International Classes:
C12N15/09; A61K39/395; A61P29/00; A61P35/00; A61P37/02; C07K16/28; C07K17/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; G01N33/53; C12P21/08
Domestic Patent References:
| WO2010013498A1 | 2010-02-04 |
Foreign References:
| JP3118460B2 | 2000-12-18 | |||
| US4816567A | 1989-03-28 |
Other References:
FORTIER I. ET AL.: "Immunolocalization of the prostaglandin E2 receptor subtypes in human bone tissue: differences in foetal, adult normal, osteoporotic and pagetic bone", PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, vol. 70, 2004, pages 431 - 439, XP055084723
BISWAS, S. ET AL.: "Prostaglandin E2 receptor subtypes, EPI, EP2, EP3 and EP4 in human and mouse ocular tissues-a comparative immunohistochemical study", PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, vol. 71, 2004, pages 277 - 288, XP004571548
STILLMAN B.A. ET AL.: "A conserved threonine in the second extracellular loop of the human EP2 and EP4 receptors is required for ligand binding", EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 357, 1998, pages 73 - 82, XP055084727
FEDYK, E. R. ET AL.: "Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells", PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 10978 - 10983, XP001040639
TAKITA, M. ET AL.: "Prostaglandin E receptor EP4 antagonist suppresses osteolysis due to bone metastasis of mouse malignant melanoma cells", FEBS LETTERS, vol. 581, 2007, pages 565 - 571, XP005907872
LIN, C. ET AL.: "Prostaglandin E2 receptor EP4 contributes to inflammatory pain hypersensitivity", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 319, no. 3, 2006, pages 1096 - 1103, XP008163202
SUGIMOTO ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 11613 - 11617
YAO ET AL., NAT. MED., vol. 15, 2009, pages 633 - 640
SAKATA ET AL., J PHARMACOL SCI., vol. 112, no. 1, 2010, pages 1 - 5
ROBERTSON FM, CANCER., vol. 116, no. 11, 1 June 2010 (2010-06-01), pages 2806 - 14
MARTINET L., BIOCHEM PHARMACOL., vol. 80, no. 6, 15 September 2010 (2010-09-15), pages 838 - 45
SHARMA SD, MOL CANCER THER., vol. 9, no. 3, March 2010 (2010-03-01), pages 569 - 80
SHARMA S ET AL., CANCER RES., vol. 65, no. 12, 15 June 2005 (2005-06-15), pages 5211 - 20
LIN C.-R. ET AL., J PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 319, no. 3, 2006, pages 1096 - 1103
POPP L. ET AL., EUROPEAN JOURNAL OF PAIN., vol. 13, no. 7, 2009, pages 691 - 703
KOHLER; MILSTEIN, NATURE, vol. 256, 1975, pages 495
CLACKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
MARK ET AL., J. MOL. BIOL., vol. 222, 1991, pages 581 - 597
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 6851 - 6855
PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 6851 - 6855
NATURE, vol. 321, 1986, pages 522 - 525
J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
QUEEN C ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 10029 - 10033
TOMIZUKA, K. ET AL., NATURE GENETICS, vol. 16, 1997, pages 133 - 143
KUROIWA, Y., NUC. ACIDS RES., vol. 26, 1998, pages 3447 - 3448
YOSHIDA, H.: "Animal Cell Technology: Basic and Applied Aspects", vol. 10, 1999, KLUWER ACADEMIC PUBLISHERS, pages: 69 - 73
TOMIZUKA, K., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 722 - 727
WORMSTONE, I. M., INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 43, no. 7, 2002, pages 2301 - 8
CARMEN, S., BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS, vol. 1, no. 2, 2002, pages 189 - 203
SIRIWARDENA, D., OPTHALMOLOGY, vol. 109, no. 3, 2002, pages 427 - 431
KABAT ET AL.: "Sequences of Proteins of Immunological Interest, Fifth edition,", 1991, U. S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
See also references of EP 2623594A4
BISWAS, S. ET AL.: "Prostaglandin E2 receptor subtypes, EPI, EP2, EP3 and EP4 in human and mouse ocular tissues-a comparative immunohistochemical study", PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, vol. 71, 2004, pages 277 - 288, XP004571548
STILLMAN B.A. ET AL.: "A conserved threonine in the second extracellular loop of the human EP2 and EP4 receptors is required for ligand binding", EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 357, 1998, pages 73 - 82, XP055084727
FEDYK, E. R. ET AL.: "Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells", PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 10978 - 10983, XP001040639
TAKITA, M. ET AL.: "Prostaglandin E receptor EP4 antagonist suppresses osteolysis due to bone metastasis of mouse malignant melanoma cells", FEBS LETTERS, vol. 581, 2007, pages 565 - 571, XP005907872
LIN, C. ET AL.: "Prostaglandin E2 receptor EP4 contributes to inflammatory pain hypersensitivity", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 319, no. 3, 2006, pages 1096 - 1103, XP008163202
SUGIMOTO ET AL., J. BIOL. CHEM., vol. 282, 2007, pages 11613 - 11617
YAO ET AL., NAT. MED., vol. 15, 2009, pages 633 - 640
SAKATA ET AL., J PHARMACOL SCI., vol. 112, no. 1, 2010, pages 1 - 5
ROBERTSON FM, CANCER., vol. 116, no. 11, 1 June 2010 (2010-06-01), pages 2806 - 14
MARTINET L., BIOCHEM PHARMACOL., vol. 80, no. 6, 15 September 2010 (2010-09-15), pages 838 - 45
SHARMA SD, MOL CANCER THER., vol. 9, no. 3, March 2010 (2010-03-01), pages 569 - 80
SHARMA S ET AL., CANCER RES., vol. 65, no. 12, 15 June 2005 (2005-06-15), pages 5211 - 20
LIN C.-R. ET AL., J PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 319, no. 3, 2006, pages 1096 - 1103
POPP L. ET AL., EUROPEAN JOURNAL OF PAIN., vol. 13, no. 7, 2009, pages 691 - 703
KOHLER; MILSTEIN, NATURE, vol. 256, 1975, pages 495
CLACKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
MARK ET AL., J. MOL. BIOL., vol. 222, 1991, pages 581 - 597
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 6851 - 6855
PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 6851 - 6855
NATURE, vol. 321, 1986, pages 522 - 525
J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
QUEEN C ET AL., PROC. NATL. ACAD. SCI. USA, vol. 86, 1989, pages 10029 - 10033
TOMIZUKA, K. ET AL., NATURE GENETICS, vol. 16, 1997, pages 133 - 143
KUROIWA, Y., NUC. ACIDS RES., vol. 26, 1998, pages 3447 - 3448
YOSHIDA, H.: "Animal Cell Technology: Basic and Applied Aspects", vol. 10, 1999, KLUWER ACADEMIC PUBLISHERS, pages: 69 - 73
TOMIZUKA, K., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 722 - 727
WORMSTONE, I. M., INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 43, no. 7, 2002, pages 2301 - 8
CARMEN, S., BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS, vol. 1, no. 2, 2002, pages 189 - 203
SIRIWARDENA, D., OPTHALMOLOGY, vol. 109, no. 3, 2002, pages 427 - 431
KABAT ET AL.: "Sequences of Proteins of Immunological Interest, Fifth edition,", 1991, U. S. DEPARTMENT OF HEALTH AND HUMAN SERVICES
See also references of EP 2623594A4
Attorney, Agent or Firm:
OKUHARA, Koji et al. (JP)
Yasushi Okuhara (JP)
Yasushi Okuhara (JP)
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