Title:
ANTIBODY-BOUND NANOWIRE FOR EXOSOME SEPARATION, AND EXOSOME SEPARATION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/146083
Kind Code:
A1
Abstract:
The present invention relates to a composition and chromatography column for separating a high-purity exosome, and an exosome separation method using same. The present invention provides information on the pH, ion concentration conditions, and volume ratio, between an ion exchange resin and a biological sample, at which the exosome can be separated with high purity from the biological sample through the ion exchange resin. When the exosome is separated according to the information, impurities such as lipid proteins and water-soluble proteins can be effectively removed to quickly obtain the high-purity exosome at low cost.
Inventors:
PARK JI-HO (KR)
BACK WOOJIN (KR)
JUNG JIK-HAN (KR)
BACK WOOJIN (KR)
JUNG JIK-HAN (KR)
Application Number:
PCT/KR2021/020300
Publication Date:
July 07, 2022
Filing Date:
December 30, 2021
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12M1/00; B01D15/36; C12N5/078
Domestic Patent References:
| WO2012087241A1 | 2012-06-28 |
Foreign References:
| US20090232883A1 | 2009-09-17 | |||
| US6899863B1 | 2005-05-31 | |||
| KR20160115988A | 2016-10-06 |
Other References:
HEATH NIKKI, GRANT LOIS, DE OLIVEIRA TAIANA MAIA, ROWLINSON RACHEL, OSTEIKOETXEA XABIER, DEKKER NIEK, OVERMAN ROSS: "Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography", SCIENTIFIC REPORTS, vol. 8, no. 1, 1 December 2018 (2018-12-01), pages 1 - 12, XP055829537, DOI: 10.1038/s41598-018-24163-y
Attorney, Agent or Firm:
LEE, Won Hee (KR)
Download PDF: