Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
ANTIBODY FRAGMENT DEGRADING AND REMOVING ABNORMAL TDP-43
Document Type and Number:
WIPO Patent Application WO/2019/177138
Kind Code:
A1
Abstract:
Disclosed is a modified antibody fragment consisting of an antibody fragment capable of binding to misfolded TDP-43 and a chaperone-mediated autophagy transit signal peptide, wherein: the antibody fragment includes a heavy-chain variable region that comprises heavy chain CDR1 comprising the amino acid sequence GFNIKDYY (SEQ ID NO: 1), heavy chain CDR2 comprising the amino acid sequence IDPEDGET (SEQ ID NO: 2) and heavy chain CDR3 comprising the amino acid sequence TIIYYYGSRYVDY (SEQ ID NO: 3), each sequence optionally having not more than 3 amino acid substitutions, and/or a light-chain variable region that comprises light chain CDR1 comprising the amino acid sequence SSISSSY(SEQ ID NO: 4), light chain CDR2 comprising the amino acid sequence RTS and light chain CDR3 comprising the amino acid sequence QQGSSIPLT (SEQ ID NO: 5), each sequence optionally having not more than 3 amino acid substitutions; and the antibody fragment is scFv, VH or VL.

Inventors:
URUSHITANI MAKOTO (JP)
TAMAKI YOSHITAKA (JP)
Application Number:
PCT/JP2019/010771
Publication Date:
September 19, 2019
Filing Date:
March 15, 2019
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
NATIONAL UNIV CORPORATION SHIGA UNIV OF MEDICAL SCIENCE (JP)
International Classes:
C12N15/13; A61K31/7088; A61P25/00; A61P25/28; A61P43/00; C07K16/18; C07K19/00; C12N15/85
Domestic Patent References:
WO2009008529A12009-01-15
WO2013061163A22013-05-02
Foreign References:
JP2013162772A2013-08-22
JP2014171425A2014-09-22
JP2013162772A2013-08-22
Other References:
TAMAKI, Y. ET AL.: "Degradative intrabody for selective elimination of pathogenic TDP-43 aggregates in vitro and in murine embryos' cerebrum", JOURNAL OF THE NEUROLOGICAL SCIENCES, vol. 381, no. 1984, 2017, pages 715, XP085295317, ISSN: 0022-510X, doi:10.1016/j.jns.2017.08.2014
TAMAKI, YOSHITAKA ET AL.: "Elimination of TDP-43 inclusions linked to amyotrophic lateral sclerosis by a misfolding-specific intrabody with dual proteolytic signals", SCIENTIFIC REPORTS, vol. 8, no. 6030, 16 April 2018 (2018-04-16), XP055638518, ISSN: 2045-2322
SAMBROOKRUSSELL: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3770262A4
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Download PDF: