Title:
ANTIBODY FUSED WITH LABELING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/025826
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel antibody that has excellent properties inherent to antibodies and enables a labeling protein fused therewith to sufficiently function. Provided is an Fab antibody fused with a labeling protein, wherein one of a pair of peptides that constitute a leucine zipper is added to the C-terminus of the H-chain and the other of said pair is added to the C-terminus of the L-chain respectively and the labeling protein is attached to the H-chain and/or the L-chain via the peptide(s).
Inventors:
NAKANO HIDEO (JP)
MORI AKIHIRO (JP)
KATO TERUYO (JP)
MORI AKIHIRO (JP)
KATO TERUYO (JP)
Application Number:
PCT/JP2017/027791
Publication Date:
February 08, 2018
Filing Date:
August 01, 2017
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
International Classes:
C12N15/09; C07K19/00; C12P21/08
Domestic Patent References:
| WO2016204198A1 | 2016-12-22 |
Foreign References:
| JP2016002009A | 2016-01-12 | |||
| JP2010519894A | 2010-06-10 | |||
| US20090035821A1 | 2009-02-05 |
Other References:
HIDEO NAKANO ET AL.,: "Zipbody: Leucine Zipper o Mochiita Fab Kotai to Sono Riyo", DAI 5 KAI NEO BIO BUNSHI KENKYUKAI YOSHISHU, 10 June 2016 (2016-06-10), Retrieved from the Internet [retrieved on 20170831]
OJIMA-KATO TERUYO. ET AL.: "`Zipbody' leucine zipper-fused Fab in E.coli in vitro and in vivo expression systems", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 29, 21 February 2016 (2016-02-21), pages 149 - 157, XP055604740
BIVONA, L. ET AL.: "Influence of the second amino acid on recombinant protein expression", PROTEIN EXPRESSION AND PURIFICATION, vol. 74, 2010, pages 248 - 256, XP027394798, DOI: doi:10.1016/j.pep.2010.06.005
OJIMA-KATO TERUYO. ET AL.: "`Zipbody' leucine zipper-fused Fab in E.coli in vitro and in vivo expression systems", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 29, 21 February 2016 (2016-02-21), pages 149 - 157, XP055604740
BIVONA, L. ET AL.: "Influence of the second amino acid on recombinant protein expression", PROTEIN EXPRESSION AND PURIFICATION, vol. 74, 2010, pages 248 - 256, XP027394798, DOI: doi:10.1016/j.pep.2010.06.005
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
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