Title:
ANTIBODY TYPE BIOLOGICAL MAGNETIC MICROSPHERE, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/111661
Kind Code:
A1
Abstract:
Provided are an antibody type biological magnetic microsphere, a preparation method therefor and the use thereof. In the first aspect, provided is a biological magnetic microsphere. At least one polymer with a linear main chain and branched chain is fixed to the outer surface of the magnetic microsphere body, and a tail end of the branched chain of the polymer fixed on the biological magnetic microsphere is connected to an antibody type tag. Further provided are a method for preparing the biological magnetic microsphere provided on the basis of the first aspect and the use thereof. The biological magnetic microsphere is convenient in terms of operation and use and can be rapidly dispersed and rapidly settled in a solution without the need to use large-scale experimental equipment such as a high-speed centrifuge. The biological magnetic microsphere is an antibody magnetic bead, and the antibody type tag can also be connected to the tail ends of the branched chains of the polymer by means of the interaction of an affinity compound. The biological magnetic microsphere can be extensively used, the antibody type label, as a purification medium, has flexible selectivity, and the biological magnetic microsphere is universally used for the separation and purification of a target object on a large scale.
Inventors:
GUO MIN (CN)
XU LIQIONG (CN)
CAO PINSHENG (CN)
TANG LEI (CN)
YU XUE (CN)
XU LIQIONG (CN)
CAO PINSHENG (CN)
TANG LEI (CN)
YU XUE (CN)
Application Number:
PCT/CN2021/133735
Publication Date:
June 02, 2022
Filing Date:
November 26, 2021
Export Citation:
Assignee:
KANGMA HEALTHCODE SHANGHAI BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K1/14; C12P21/00
Foreign References:
| CN113564214A | 2021-10-29 | |||
| CN113564213A | 2021-10-29 | |||
| CN112877387A | 2021-06-01 | |||
| CN112111042A | 2020-12-22 | |||
| CN110261603A | 2019-09-20 | |||
| CN101955231A | 2011-01-26 | |||
| CN103157447A | 2013-06-19 | |||
| CN102500336A | 2012-06-20 | |||
| KR20120077616A | 2012-07-10 |
Other References:
XU PENG;LIANG XUGUANG;CAO XIAOYONG;LIU SONGCAN;WANG LIYING;GAO QINWEI: "Preparation of Fe3O4@SiO2@PAA Multilayer Core-Shell Structural Composite Microspheres and Its Dye Adsorption Performance", JOURNAL OF FORESTRY ENGINEERING, vol. 3, no. 3, 18 May 2018 (2018-05-18), pages 52 - 56, XP055934299, ISSN: 2096-1359, DOI: 10.13360/j.issn.2096-1359.2018.03.009
MARUTANI, E. YAMAMOTO, S. NINJBADGAR, T. TSUJII, Y. FUKUDA, T. TAKANO, M.: "Surface-initiated atom transfer radical polymerization of methyl methacrylate on magnetite nanoparticles", POLYMER, vol. 45, no. 7, 1 March 2004 (2004-03-01), AMSTERDAM, NL, pages 2231 - 2235, XP004493340, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2004.02.005
MARUTANI, E. YAMAMOTO, S. NINJBADGAR, T. TSUJII, Y. FUKUDA, T. TAKANO, M.: "Surface-initiated atom transfer radical polymerization of methyl methacrylate on magnetite nanoparticles", POLYMER, vol. 45, no. 7, 1 March 2004 (2004-03-01), AMSTERDAM, NL, pages 2231 - 2235, XP004493340, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2004.02.005
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