Title:
ANTIDOTE
Document Type and Number:
WIPO Patent Application WO/2020/122090
Kind Code:
A1
Abstract:
The present invention relates to: a toehold sequence-added nucleic acid aptamer wherein in a nucleic acid aptamer consisting of a single-stranded loop portion and a double-stranded stem portion, a nucleic acid sequence (toehold sequence), which is not complementary to the sequence of the nucleic acid aptamer, is connected to an end of one of two strands of the stem portion; or an antidote wherein in a nucleic acid that has a nucleic sequence complementary to the nucleic acid aptamer consisting of the single-stranded loop portion and the double-stranded stem portion, a nucleic acid having a nucleic acid sequence complementary to the toehold is connected to an end different from said end.
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Inventors:
FURUSHO HITOSHI (JP)
YOSHIMOTO KEITARO (JP)
WAKUI KOJI (JP)
YOSHITOMI TORU (JP)
MIYAKAWA MASATO (JP)
YOSHIMOTO KEITARO (JP)
WAKUI KOJI (JP)
YOSHITOMI TORU (JP)
MIYAKAWA MASATO (JP)
Application Number:
PCT/JP2019/048382
Publication Date:
June 18, 2020
Filing Date:
December 11, 2019
Export Citation:
Assignee:
NISSAN CHEMICAL CORP (JP)
UNIV TOKYO (JP)
UNIV TOKYO (JP)
International Classes:
A61K31/7088; A61K48/00; A61P39/02; C12N15/115
Domestic Patent References:
| WO2018053427A1 | 2018-03-22 | |||
| WO2016016259A1 | 2016-02-04 | |||
| WO2019146676A1 | 2019-08-01 |
Foreign References:
| US20110275701A1 | 2011-11-10 | |||
| US20110172292A1 | 2011-07-14 |
Other References:
TASSET, D. M. ET AL.: "Oligonucleotide Inhibitors of Human Thrombin that Bind Distinct Epitopes", J. MOL. BIOL., vol. 272, 1997, pages 688 - 698, XP004453692, DOI: 10.1006/jmbi.1997.1275
ZHQU, J. ET AL.: "Aptamers as targeted therapeutics: current potential and challenges", NATURE REVIEWS DRUG DISCOVERY, vol. 16, 2017, pages 181 - 202, XP055649283, DOI: 10.1038/nrd.2016.199
LI, J. ET AL.: "Aptamer proximity recognition- dependent strand translocation for enzyme-free anc amplified fluorescent detection of thrombin via catalytic hairpin assembly", ANALYTICA CHIMICA ACTA, vol. 1038, 6 July 2018 (2018-07-06), pages 126 - 131
WAKUI, K. ET AL.: "Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX", MOLECULAR THERAPY: NUCLEIC ACIDS, vol. 16, June 2019 (2019-06-01), pages 348 - 359, XP055604239, DOI: 10.1016/j.omtn.2019.03.002
ZHQU, J. ET AL.: "Aptamers as targeted therapeutics: current potential and challenges", NATURE REVIEWS DRUG DISCOVERY, vol. 16, 2017, pages 181 - 202, XP055649283, DOI: 10.1038/nrd.2016.199
LI, J. ET AL.: "Aptamer proximity recognition- dependent strand translocation for enzyme-free anc amplified fluorescent detection of thrombin via catalytic hairpin assembly", ANALYTICA CHIMICA ACTA, vol. 1038, 6 July 2018 (2018-07-06), pages 126 - 131
WAKUI, K. ET AL.: "Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX", MOLECULAR THERAPY: NUCLEIC ACIDS, vol. 16, June 2019 (2019-06-01), pages 348 - 359, XP055604239, DOI: 10.1016/j.omtn.2019.03.002
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
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