Title:
ANTIFIBROTIC AGENT
Document Type and Number:
WIPO Patent Application WO/2020/226038
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a medicine that is effective for inhibiting hepatic fibrosis. A modified cytoglobin protein comprising: a cytoglobin protein comprising at least one polypeptide selected from among (1) a polypeptide which contains the amino acid sequence represented by SEQ ID NO: 1, (2) a polypeptide which contains an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by substitution, addition, insertion or deletion of one to several amino acid residues and which is capable of inhibiting hepatic fibrosis, and (3) a polypeptide which contains an amino acid sequence showing a sequence identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 1 and which is capable of inhibiting hepatic fibrosis; and an oligohistidine tag and/or a TAT peptide tag attached to the cytoglobin protein. This modified cytoglobin protein is usable as an active ingredient of an antifibrotic agent.
Inventors:
KAWADA NORIFUMI (JP)
LE THI THAN THUY (JP)
LE THI THAN THUY (JP)
Application Number:
PCT/JP2020/016590
Publication Date:
November 12, 2020
Filing Date:
April 15, 2020
Export Citation:
Assignee:
UNIV OSAKA PUBLIC CORP (JP)
International Classes:
A61P1/16; A61K38/17; A61K47/64; A61P35/00; A61P43/00; C07K7/06; C07K7/08; C07K14/47; C07K19/00; C12N15/62
Foreign References:
| CN101863985A | 2010-10-20 |
Other References:
NGUYEN, THI THANH HAI ET AL.: "Protective role of hepatic stellate cell-specific overexpression of cytoglobin against liver fibrosis in in vitro and in vivo", vol. 59, no. 1, 2018, pages A391
NGUYEN, THI THANH HAI ET AL., CYTOGLOBIN OVEREXPRESSION PROTECTS MICE AGAINST TAA-INDUCED LIVER FIBROSIS DEVELOPMENT, vol. 58, no. 3, 2017, pages A782
LE, THI THANH THUY ET AL.: "Cytoglobin suppresses liver injury and fibrosis in bile duct ligated mice by regulating nitrosative and oxidative stress", HEPATOLOGY, vol. 68, no. 1, 2018, pages 128A - 129A
YOSHIZATO KATSUTOSHI, THUY LE THI THANH, SHIOTA GOSHI, KAWADA NORIFUMI: "Discovery of cytoglobin and its roles in physiology and pathology of hepatic stellate cells", PROCEEDINGS OF THE JAPAN ACADEMY, vol. 92, no. 3, 2016, pages 77 - 97, XP055760392, ISSN: 0386-2208
ASAHINA, K. ET AL.: "Characterization of human stellate cell activation-associated protein and its expression in human liver", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1577, 2002, pages 471 - 475, XP004383779, DOI: 10.1016/S0167-4781(02)00477-3
NGUYEN, THI THANH HAI ET AL., CYTOGLOBIN OVEREXPRESSION PROTECTS MICE AGAINST TAA-INDUCED LIVER FIBROSIS DEVELOPMENT, vol. 58, no. 3, 2017, pages A782
LE, THI THANH THUY ET AL.: "Cytoglobin suppresses liver injury and fibrosis in bile duct ligated mice by regulating nitrosative and oxidative stress", HEPATOLOGY, vol. 68, no. 1, 2018, pages 128A - 129A
YOSHIZATO KATSUTOSHI, THUY LE THI THANH, SHIOTA GOSHI, KAWADA NORIFUMI: "Discovery of cytoglobin and its roles in physiology and pathology of hepatic stellate cells", PROCEEDINGS OF THE JAPAN ACADEMY, vol. 92, no. 3, 2016, pages 77 - 97, XP055760392, ISSN: 0386-2208
ASAHINA, K. ET AL.: "Characterization of human stellate cell activation-associated protein and its expression in human liver", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1577, 2002, pages 471 - 475, XP004383779, DOI: 10.1016/S0167-4781(02)00477-3
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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