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Title:
ANTIFOULING COMPOSITION PREPARED FROM A PSEUDOMONAS PF-11 CULTURE
Document Type and Number:
WIPO Patent Application WO/2016/198950
Kind Code:
A3
Abstract:
This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.

Inventors:
COSTA GONÇALO (PT)
FREIRE PATRICK (PT)
SANTOS ROMANA (PT)
SILVA ANA CRISTINA (PT)
GUINOTE INES (PT)
Application Number:
PCT/IB2016/000913
Publication Date:
February 23, 2017
Filing Date:
June 09, 2016
Export Citation:
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Assignee:
BIOMIMETX S A (PT)
COSTA GONÇALO (PT)
FREIRE PATRICK (PT)
SANTOS ROMANA (PT)
SILVA ANA CRISTINA (PT)
GUINOTE INES (PT)
International Classes:
C12N1/02; A01N63/27
Other References:
ALEMU, F. & ALEMU, T.: "Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia", AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, vol. 7, no. 47, 28 November 2013 (2013-11-28), pages 5364 - 5373, XP002764078
KHEIRANDISH, Z. & HARIGHI, B.: "Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato", BIOLOGICAL CONTROL, vol. 86, 6 April 2015 (2015-04-06), pages 14 - 19, XP002764079
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE; 2004, AHMADZADEH, M. ET AL.: "Study on production of some antimicrobial metabolites by flourescent pseudomonads", XP002764080, Database accession no. 2004:198020
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE; 2003, AHMADZADEH, M. ET AL.: "Effects of fluorescent pseudomonads on Pythium ultimum casual agent of seed rot of common bean", XP002764081, Database accession no. 2004:75062
MIYAJI, T ET. AL.: "Purification and characterization of extracellular alkaline serine protease from Stenotrophomonas maltophilia strain S-1", LETTERS IN APPLIED MICROBIOLOGY, vol. 41, no. 3, 2005, pages 253 - 257, XP002504921
DIXIT, R. ET AL.: "Screening of antagonistic potential and plant growth promotion activities of Trichoderma spp. and fluorescent Pseudomonas spp. isolates against Sclerotinia sclerotiorum causing stem rot of French bean", LEGUME RESEARCH, vol. 38, no. 3, 19 June 2015 (2015-06-19), pages 375 - 381, XP055318235
Attorney, Agent or Firm:
GERSHIK, Gary, J. (US)
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