Title:
ANTIMICROBIAL AGENTS
Document Type and Number:
WIPO Patent Application WO/2010/023207
Kind Code:
A3
Abstract:
The present invention relates to endolysin variants comprising an endolysin to which a peptide stretch with membrane or LPS disrupting activity is fused. Moreover, the present invention relates to nucleic acid molecules encoding said modified endolysin variant, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to a method for producing said endolysin variant. Further, the present invention relates to said modified endolysin variant for use as a medicament, in particular for the treatment or prevention of Gram- negative bacterial infections, as diagnostic means, disinfectant or as cosmetic substance. The present invention also relates to the removal or reduction or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to the use of said endolysin variant as a diagnostic means in medicinal, food or feed or environmental diagnostic. Finally, the present invention relates to a pharmaceutical composition comprising said modified endolysin variant
Inventors:
BRIERS YVES (CH)
LAVIGNE ROB (BE)
VOLCKAERT GUIDO (BE)
LAVIGNE ROB (BE)
VOLCKAERT GUIDO (BE)
Application Number:
PCT/EP2009/060947
Publication Date:
July 15, 2010
Filing Date:
August 25, 2009
Export Citation:
Assignee:
UNIV LEUVEN KATH (BE)
BRIERS YVES (CH)
LAVIGNE ROB (BE)
VOLCKAERT GUIDO (BE)
BRIERS YVES (CH)
LAVIGNE ROB (BE)
VOLCKAERT GUIDO (BE)
International Classes:
C12N9/50; A61K38/43; A61P31/04; C12N9/52
Foreign References:
| DE102006061002A1 | 2008-06-26 | |||
| EP0510907A2 | 1992-10-28 | |||
| US20020127220A1 | 2002-09-12 | |||
| US5985271A | 1999-11-16 |
Other References:
ARIMA H ET AL: "Bactericidal action of lysozymes attached with various sizes of hydrophobic peptides to the C-terminal using genetic modification", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL LNKD- DOI:10.1016/S0014-5793(97)01071-5, vol. 415, no. 1, 22 September 1997 (1997-09-22), pages 114 - 118, XP004261147, ISSN: 0014-5793
IBRAHIM H R ET AL: "Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 18 FEB 1994 LNKD- PUBMED:8106483, vol. 269, no. 7, 18 February 1994 (1994-02-18), pages 5059 - 5063, XP002579297, ISSN: 0021-9258
LOESSNER M J: "Bacteriophage endolysins - current state of research and applications", CURRENT OPINION IN MICROBIOLOGY, CURRENT BIOLOGY LTD, GB LNKD- DOI:10.1016/J.MIB.2005.06.002, vol. 8, no. 4, 1 August 2005 (2005-08-01), pages 480 - 487, XP004989436, ISSN: 1369-5274
ORITO Y ET AL: "Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY JUL 2004 LNKD- PUBMED:14714151, vol. 65, no. 1, July 2004 (2004-07-01), pages 105 - 109, XP002579253, ISSN: 0175-7598
MORITA M ET AL: "Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL LNKD- DOI:10.1016/S0014-5793(01)02587-X, vol. 500, no. 1-2, 29 June 2001 (2001-06-29), pages 56 - 59, XP004251377, ISSN: 0014-5793
IBRAHIM H R ET AL: "Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 18 FEB 1994 LNKD- PUBMED:8106483, vol. 269, no. 7, 18 February 1994 (1994-02-18), pages 5059 - 5063, XP002579297, ISSN: 0021-9258
LOESSNER M J: "Bacteriophage endolysins - current state of research and applications", CURRENT OPINION IN MICROBIOLOGY, CURRENT BIOLOGY LTD, GB LNKD- DOI:10.1016/J.MIB.2005.06.002, vol. 8, no. 4, 1 August 2005 (2005-08-01), pages 480 - 487, XP004989436, ISSN: 1369-5274
ORITO Y ET AL: "Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY JUL 2004 LNKD- PUBMED:14714151, vol. 65, no. 1, July 2004 (2004-07-01), pages 105 - 109, XP002579253, ISSN: 0175-7598
MORITA M ET AL: "Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL LNKD- DOI:10.1016/S0014-5793(01)02587-X, vol. 500, no. 1-2, 29 June 2001 (2001-06-29), pages 56 - 59, XP004251377, ISSN: 0014-5793
Attorney, Agent or Firm:
DEHMEL, Albrecht (Munich, DE)
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