Title:
ANTIVIRAL COMPOSITION AGAINST HEPATITIS B VIRUS, INCLUDING INTERLEUKIN-32 AS ACTIVE INGREDIENT
Document Type and Number:
WIPO Patent Application WO/2018/101745
Kind Code:
A1
Abstract:
The present invention relates to an antiviral composition against hepatitis B virus, which includes an interleukin-32 (IL-32) gene or an IL-32 protein, which is an inhibitor of expression or activation of hepatocyte nuclear factor 4 alpha (HNF4α) or hepatocyte nuclear factor 1 alpha (HNF1α), as an active ingredient, and a method for screening an antiviral substance against the virus. The inventors experimentally confirmed an inhibitory effect on viral DNA replication and expression by reducing the expression of host transcription factors such as HNF4α and HNF1α, involved in the transcription of the viral gene by increasing ERK1/2 phosphorylation due to IL-32 secretion induced by TNF-α and IFN-γ cytokines secreted from infected cells when host cells were infected with the hepatitis B virus, and therefore an IL-32-mediated antiviral effect on hepatitis B virus and a molecular mechanism thereof were identified. Therefore, the antiviral activity of IL-32 against hepatitis B virus newly identified according to the present invention may provide a new understanding for the development of therapeutic agent against the virus, and may be effectively used in the development of an antiviral agent.
Inventors:
KIM KYUN-HWAN (KR)
KIM DOO-HYUN (KR)
PARK YEONG-MIN (KR)
KIM DOO-HYUN (KR)
PARK YEONG-MIN (KR)
Application Number:
PCT/KR2017/013837
Publication Date:
June 07, 2018
Filing Date:
November 29, 2017
Export Citation:
Assignee:
DANDI BIOSCIENCE INC (KR)
International Classes:
A61K48/00; A61K38/20; C12Q1/68; G01N33/50; G01N33/68
Foreign References:
| CN101703762A | 2010-05-12 |
Other References:
LI, YONGKUI ET AL.: "Inducible interleukin 32 ( IL -32) exerts extensive antiviral function via selective stimulation of interferon lambda1 (IFN- lambda1", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 288, no. 29, 19 July 2013 (2013-07-19), pages 20927 - 20941, XP055491781, Retrieved from the Internet
DATABASE NCBI 10 September 2016 (2016-09-10), Database accession no. NM_001012633.1
DATABASE NCBI 10 September 2016 (2016-09-10), Database accession no. NP_001012651.1
PAN, XINGFEI ET AL.: "Interleukin-32 expression induced by hepatitis B virus protein X is mediated through activation of NF-KB", MOLECULAR IMMUNOLOGY, vol. 48, no. 12, 1 July 2011 (2011-07-01), pages 1573 - 1577, XP028225649, Retrieved from the Internet
DATABASE NCBI 10 September 2016 (2016-09-10), Database accession no. NM_001012633.1
DATABASE NCBI 10 September 2016 (2016-09-10), Database accession no. NP_001012651.1
PAN, XINGFEI ET AL.: "Interleukin-32 expression induced by hepatitis B virus protein X is mediated through activation of NF-KB", MOLECULAR IMMUNOLOGY, vol. 48, no. 12, 1 July 2011 (2011-07-01), pages 1573 - 1577, XP028225649, Retrieved from the Internet
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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