Title:
APPARATUS FOR ASSAY, SYNTHESIS AND STORAGE, AND METHODS OF MANUFACTURE, USE, AND MANIPULATION THEREOF
Document Type and Number:
WIPO Patent Application WO2002030561
Kind Code:
A3
Abstract:
The invention features methods of making devices, or "platens" having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analizying the contents of the array, including assaying of physical properties of the samples. In various embodiments, the reagents can be contained within the through-holes by capillary action, attached to the walls of the through-hole. The porous material can be, for example, a gel, a bead, sintered glass, or particulate matter, or can be the inner wall of a ghrough-hole that has been chemically etched. In particular embodiments, the arrays can include invidual molecules, complexes of molecules, viruses, cells, groups of cells, pieces of tissue, or small particles or beads. The members of the arrays can aslo, for example, function as transducers that report the presence of an analyte (e.g., by providing an easily detected signal), or they can function as selective binding agents for the retention of analytes of interest. Using these methods, arrays corresponding to a large plurality of human genes (e.g., using nucleic acid probes) can also be prepared.
Inventors:
HESS ROBERT A (US)
KANIGAN TANYA S (US)
BRENAN COLIN J H (US)
OZBAL CAN (US)
LINTON JOHN DUDLEY (US)
KANIGAN TANYA S (US)
BRENAN COLIN J H (US)
OZBAL CAN (US)
LINTON JOHN DUDLEY (US)
Application Number:
PCT/US2001/031770
Publication Date:
May 22, 2003
Filing Date:
October 10, 2001
Export Citation:
Assignee:
BIOTROVE INC (US)
HESS ROBERT A (US)
KANIGAN TANYA S (US)
BRENAN COLIN J H (US)
OZBAL CAN (US)
LINTON JOHN DUDLEY (US)
HESS ROBERT A (US)
KANIGAN TANYA S (US)
BRENAN COLIN J H (US)
OZBAL CAN (US)
LINTON JOHN DUDLEY (US)
International Classes:
G01N21/78; B01D61/00; B01D71/20; B01J19/00; B01L3/00; B01L3/02; B01L99/00; C12M1/00; C12N5/02; C12N15/09; C12Q1/02; C12Q1/68; G01N30/46; G01N35/02; G01N37/00; B81B1/00; C40B60/14; G01N30/60; G01N30/82; G01N30/84; (IPC1-7): B01J19/00
Domestic Patent References:
| WO1999055461A1 | 1999-11-04 | |||
| WO2000056456A1 | 2000-09-28 |
Foreign References:
| US5152060A | 1992-10-06 |
Other References:
PATENT ABSTRACTS OF JAPAN vol. 012, no. 348 (E - 659) 19 September 1988 (1988-09-19)
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