Title:
APPARATUS FOR HIGH-THROUGHPUT RAPID TRAPPING OF CIRCULATING TUMOR CELLS AND METHOD FOR PURIFYING CIRCULATING TUMOR CELLS
Document Type and Number:
WIPO Patent Application WO/2018/064933
Kind Code:
A1
Abstract:
An apparatus for high-throughput rapid trapping of circulating tumor cells and a method for purifying circulating tumor cells, relating to the field of biotechnology. The apparatus for high-throughput rapid trapping of circulating tumor cells comprises: a filter module, the filter module comprising a filter, a filter membrane and a negative pressure device which are arranged inside the filter and which are sealed with an inner wall of the filter; the negative pressure device is connected with an end of an outlet of the filter, and used for maintaining a negative pressure within the filter; when a negative pressure is maintained within the filter by the negative pressure device, the filter membrane is configured to trap circulating tumor cells, then stain and wash the trapped cells by using reagents which are automatically loaded by means of a reagent loading module. The apparatus may automatically control loading a sample liquid to the filter as well as the negative pressure device, such that the process in which the circulating tumor cells are trapped by using the filter membrane and then stained has the advantages of being highly automated and having a high throughput (which can be achieved by means of increasing the number of filters).
Inventors:
CHEN JING (CN)
Application Number:
PCT/CN2017/102823
Publication Date:
April 12, 2018
Filing Date:
September 22, 2017
Export Citation:
Assignee:
CHEN JING (CN)
International Classes:
C12M1/36; C12M1/12; C12N5/09
Foreign References:
| CN106282001A | 2017-01-04 | |||
| CN106520543A | 2017-03-22 | |||
| CN206396221U | 2017-08-11 | |||
| CN205398624U | 2016-07-27 | |||
| CN102439131A | 2012-05-02 | |||
| CN105699641A | 2016-06-22 | |||
| CN103347595A | 2013-10-09 | |||
| CN201610881083A | 2016-10-09 | |||
| CN201611157990A | 2016-12-15 |
Other References:
See also references of EP 3524668A4
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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