Title:
APPARATUS AND METHOD FOR HIGH THROUGHPUT PARALLEL NUCLEIC ACID SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2020/211069
Kind Code:
A1
Abstract:
A method and an apparatus for nucleic acid sequencing are provided. The method includes immobilizing capturing oligonucleotides with different sequences in reaction wells, immobilizing single-stranded nucleic acid templates in the reaction wells via annealing between the templates and the capturing oligonucleotides, amplifying the immobilized nucleic acid templates and producing a population of template clones annealed with a plurality of sequencing primers. The method further includes sequentially disposing different types of nucleotide trisphosphates, detecting, by ion-sensitive field-effect transistors, ion concentration change in the reaction wells in response to incorporation of one of the nucleotide trisphosphates at 3' end of sequencing primers, when the nucleotide trisphosphates is complementary to a corresponding nucleotide in the template clones, and sequencing the template clones by repeating the sequentially disposing and the detecting. A method for producing single-stranded nucleic acid template clones on a reaction well array is also provided.
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Inventors:
DENG DAVID XINGFEI (US)
Application Number:
PCT/CN2019/083381
Publication Date:
October 22, 2020
Filing Date:
April 19, 2019
Export Citation:
Assignee:
ARDENT BIOMED GUANGDONG CO LTD (CN)
International Classes:
G01N27/414; C12Q1/68
Domestic Patent References:
| WO2000075374A1 | 2000-12-14 | |||
| WO2017218979A1 | 2017-12-21 |
Foreign References:
| CN106460043A | 2017-02-22 | |||
| CN107254510A | 2017-10-17 |
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