Title:
APPARATUS AND METHOD FOR SEPARATING AN ANALYTE
Document Type and Number:
WIPO Patent Application WO2003019172
Kind Code:
A3
Abstract:
The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an immobilized chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.
Inventors:
MICHEL PHILIPPE (CH)
REYMOND FREDERIC (CH)
ROSSIER JOEL STEPHANE (CH)
REYMOND FREDERIC (CH)
ROSSIER JOEL STEPHANE (CH)
Application Number:
PCT/EP2002/010369
Publication Date:
December 18, 2003
Filing Date:
August 30, 2002
Export Citation:
Assignee:
DIAGNOSWISS SA (CH)
MICHEL PHILIPPE (CH)
REYMOND FREDERIC (CH)
ROSSIER JOEL STEPHANE (CH)
MICHEL PHILIPPE (CH)
REYMOND FREDERIC (CH)
ROSSIER JOEL STEPHANE (CH)
International Classes:
B01D57/02; B03C5/00; C07K1/28; C12M1/34; C12M1/38; C12M1/42; G01N27/447; (IPC1-7): G01N27/447
Domestic Patent References:
| WO2001086279A1 | 2001-11-15 | |||
| WO2001075432A2 | 2001-10-11 | |||
| WO2001068225A1 | 2001-09-20 | |||
| WO2000074850A2 | 2000-12-14 |
Other References:
See also references of EP 1421373A2
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