Title:
APPARATUSES AND METHODS FOR ASSAYING ANALYTES USING PHOTOELECTROCHEMICAL LABELS
Document Type and Number:
WIPO Patent Application WO/2004/046721
Kind Code:
A1
Abstract:
The present invention provides apparatus, kits and methods for assaying analytes using photoelectrochemical molecules as labels. Specifically, the present invention provides a method for assaying an analyte, comprising: a) contacting a sample suspected of containing an analyte with a reactant capable of binding and/or reacting with the analyte under suitable conditions to allow binding of analyte, if present in the sample, to the reactant; and b) assessing binding and/or reacting between the analyte and the reactant to determine presence and/or amount of analyte in the sample, wherein the reactant, analyte, or additional reactant or additional analyte or analyte analog is labeled with a photoelectrochemically active molecule. The assessing step also comprises converting the photoelectrochemically active molecule with light to an excited state in the presence of an electrode, and assessing an electric current generated by an electron transfer between the excited photoelectrochemically active molecule and the electrode.
Inventors:
GUO LIANGHONG (CN)
DONG DONG (CN)
ZHENG DONG (CN)
WANG FUQUAN (CN)
YANG XIQIANG (CN)
CHENG JING (CN)
DONG DONG (CN)
ZHENG DONG (CN)
WANG FUQUAN (CN)
YANG XIQIANG (CN)
CHENG JING (CN)
Application Number:
PCT/CN2003/000327
Publication Date:
June 03, 2004
Filing Date:
May 06, 2003
Export Citation:
Assignee:
CAPITAL BIOCHIP CO LTD (CN)
UNIV TSINGHUA (CN)
GUO LIANGHONG (CN)
DONG DONG (CN)
ZHENG DONG (CN)
WANG FUQUAN (CN)
YANG XIQIANG (CN)
CHENG JING (CN)
UNIV TSINGHUA (CN)
GUO LIANGHONG (CN)
DONG DONG (CN)
ZHENG DONG (CN)
WANG FUQUAN (CN)
YANG XIQIANG (CN)
CHENG JING (CN)
International Classes:
C12Q1/68; G01N33/58; (IPC1-7): G01N33/543; C12Q1/68; G01N27/327
Domestic Patent References:
| WO2004046369A2 | 2004-06-03 |
Foreign References:
| CN1325490A | 2001-12-05 | |||
| CN1249815A | 2000-04-05 | |||
| US4293310A | 1981-10-06 | |||
| US6140138A | 2000-10-31 | |||
| US6066448A | 2000-05-23 | |||
| US4293310A | 1981-10-06 |
Other References:
See also references of EP 1567866A4
WEBER ET AL., CLIN. CHEM., vol. 29, 1983, pages 1665 - 1672
NAKAMURA ET AL.: "Light-to-Electric Conversion as a New Method for DNA Duplex detection", ANALYTICAL SCIENCES, vol. 17, 2001, XP002398433
BARD; FAULKNER: "Electrochemical Methods", 1980, WILEY
WEBER ET AL., CLIN. CHEM., vol. 29, 1983, pages 1665 - 1672
NAKAMURA ET AL.: "Light-to-Electric Conversion as a New Method for DNA Duplex detection", ANALYTICAL SCIENCES, vol. 17, 2001, XP002398433
BARD; FAULKNER: "Electrochemical Methods", 1980, WILEY
Attorney, Agent or Firm:
JEEKAI & PARTNERS (Jinyu Tower A129 West Xuan Wu Men Stree, XiCheng District Beijing 1, CN)
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