Field of the invention

The present invention relates to an application device for positioning an absorptive matrix (AM) -element within a cavity of a human or animal body and to a collection kit comprising the application device . The present invention further relates to the use of the application device or the collection kit for collecting nasal secretion .

Background of the invention

Nasal secretion from the nasal cavity comprises a number of proteins . These proteins are , for example , biological markers of inflammatory processes . Accordingly, the analysi s of nasal secretion is a field of scienti fic and medical interest . For investigating inflammatory processes and allergies , the analysis is generally performed on secretion collected from the lower portion and the middle portion of the nasal cavity .

For collecting nasal secretion, di f ferent sampling techniques such as nose-blowing, suction, nasal lavage and nasosorption are used . For nasosorption, an absorptive material such as a sponge , a cotton swab or a synthetic absorptive matrix ( SAM) -element is placed within the nasal cavity for a short period of time , for example for one minute . For collecting nasal secretion from the upper portion of the nasal cavity, more speci fically from the olfactory cleft , positioning the absorptive material is generally challenging because the ol factory cleft is located in the upper part of the nose , which is di f ficult to reach and dangerously close to the base of the skull .

It is known that the di f ferent sampling techniques lead to a considerable variability in the amount of proteins captured . For example , nasal lavage leads to an uncontrollable dilution of the secretion and to a possible loss of markers via the mouth/naso- pharynx . This hinders further research in the field since data from di f ferent studies using di f ferent sampling techniques cannot be compared . Even the same sampling technique may cause variability in the nasal secretion collected . In the case of nasosorption, for example , variability may result from the di fficulty of correctly and reproducibly positioning the absorptive material at the intended target position, for example in the olfactory cleft , and/or from contamination of the absorptive material caused by inj ury or irritation of the nasal mucosa during the insertion of the material into the nose .

Despite the known problems associated with collecting nasal secretion, there is no standardi zed procedure for col lecting nasal secretion, in particular for collecting nasal secretion from the upper portion of the nasal cavity . Likewise , there is no tool for collecting nasal secretion in a standardi zed manner .

Therefore , new tools for collecting nasal secretion are needed .

Summary of the invention

In a first aspect , the present invention relates to an application device applicable for positioning an absorptive matrix (AM) -element within a cavity of a human or animal body, the application device comprising : - a storage and release tube having a distal end and a proximal end, the tube is configured to store and release an AM- element ;

- the AM-element being stored within an inner space of the tube ;

- a release element being slidably supported with respect to the tube along a longitudinal axis of the tube , the release element is configured to release the AM-element from the tube ;

- at least one deflection means arranged at the distal end of the tube , the deflection means is capable to bend the AM- element away from the longitudinal axis of the tube when the AM-element is released from the tube , wherein the AM-element is not attached to any of the tube , the release element and the deflection means .

In a second aspect , the present invention relates to a collection kit applicable for collecting biological secretion from a cavity of a human or animal body comprising the application device of the invention, wherein the collection kit further comprises a vessel arrangement for storing the AM-element after the collection of secretion .

In a third aspect , the present invention relates to the use of the application device of the invention or the collection kit of the invention for collecting nasal secretion .

Brief description of the figures

Fig . 1 shows a perspective view of a human nasal cavity in which an AM-element has been released for collecting secretion . The perspective view is shown in a semitransparent manner . Fig . 2A shows a perspective view of a first embodiment of an application device according to the invention with an AM-element in its storage ( retracted) position and an AM-element in its partially released position .

Fig . 2B shows an enlarged view of a detail X of Fig . 2A.

Fig . 3A shows a perspective view of a second embodiment of an application device according to the invention with an AM-element in its partially released position .

Fig . 3B shows an enlarged view of a detail Y of Fig . 3A.

Fig . 4 shows a perspective view of a third embodiment of an application device according to the invention with an AM-element in its storage ( retracted) position .

Fig . 5 shows an embodiment of an AM-element suitable for the application device according to the invention .

Fig . 6 shows a cross sectional view of a vessel arrangement suitable for storing and/or transporting an AM-element after the collection of secretion .

Detailed description of the invention

In a first aspect , the present invention relates to an application device applicable for positioning an absorptive matrix (AM) -element within a cavity of a human or animal body, the application device comprising :

- a storage and release tube having a distal end and a proximal end, the tube is configured to store and release an AM- element ; - the AM-element being stored within an inner space of the tube ;

- a release element being slidably supported with respect to the tube along a longitudinal axis of the tube , the release element is configured to release the AM-element from the tube ;

- at least one deflection means arranged at the distal end of the tube , the deflection means is capable to bend the AM- element away from the longitudinal axis of the tube when the AM-element is released from the tube , wherein the AM-element is not attached to any of the tube , the release element and the deflection means .

The application device of the invention is applicable for positioning the AM-element within the cavity of the human or animal body . The AM-element is to be positioned within the cavity, for example , for collecting biological secretion from the cavity in a non-invasive manner . The secretion is collected by absorption of the secretion by the AM-element . Accordingly, the application device can also be referred to as collecting device or collection device . The collection process can also be referred to as extraction process .

The absorptive matrix, also referred to as absorptive material , of the AM-element can be a synthetic absorptive matrix, a nonsynthetic absorptive matrix, or a mixture of synthetic and nonsynthetic absorptive matrices .

In case of a synthetic absorptive matrix, the AM-element is also referred to as synthetic absorptive matrix ( SAM) -element .

In case of a non- synthetic ( i . e . natural ) absorptive matrix, the matrix preferably is a cotton matrix or a cellulose matrix . The cavity preferably is a nasal cavity . The application device is particularly suitable for positioning the AM-element in the upper portion of the nasal cavity, more speci fically in the olfactory cleft , and thus for collecting nasal secretion from this region . The ol factory cleft is a narrow passage located in the upper part of each the left nasal cavity and the right nasal cavity . The ol factory cleft comprises a special type of covering, namely the ol factory epithelium, that is di f ferent from other regions of the nasal cavity . Accordingly, with few exceptions , a di f ferent type of mucous membrane surface is found in the remaining portion of the nasal cavity . The septum, the medium turbinates and the lower turbinates of the nasal cavity are not covered by the ol factory epithelium .

Accordingly, one of the most relevant applications of the application device can be seen in the positioning of the AM-element in the upper portion of the nasal cavity for collecting nasal secretion from this region . The nasal secretion collected from this region includes nasal secretion located on and in the upper layers of the ol factory epithelium . The dimensions of the AM- element are selected in a manner that facilitates collecting the nasal secretion along the entire sagittal extension and the entire width of the ol factory cleft . The analysis of nasal secretion is a field of growing scienti fic and medical interest . For example , for some diseases , the analysis of nasal secretion may be used as a non-invasive diagnostic approach that is broadly available .

The nasal secretion is preferably collected from the ol factory cleft of each the left nasal cavity and the right nasal cavity of a subj ect . To do so , one application device per cavity is applied . The collection may be performed at the same time . After collection, the secretion of the left nasal cavity and the secretion of the right nasal cavity may be combined for further analysis .

In another example , the cavity is an oral cavity . The application device is suitable for positioning the AM-element , for example , between the buccal mucosa of the cheek and the teeth in the upper or lower alveolar ridge for collecting secretion from this region . In this case , the biological secretion that is collected is saliva . After collection, the saliva may be analyzed, for example , for the presence of human papilloma virus (HPV) . HPV- associated tumors of the oral cavity, such as oral cavity squamous cell carcinoma, are a common form of cancer . The saliva may also or alternatively be analyzed for Herpes simplex virus-associated proteins .

In yet another example , the cavity is an auditory canal . In this case , the biological secretion that is collected is ceruminal liquid .

The storage and release tube has a distal end and a proximal end . The term "distal end" as used herein refers to the end which points away from the operator of the device and towards the cavity in which the device is to be inserted . Accordingly, the AM-element is to be released from the tube at the distal end or at a distal end portion of the tube . The term "proximal end" as used herein refers to the opposite end of the tube , i . e . to the end which points towards the operator of the device and away from the cavity in which the device is to be inserted . The proximal end of the tube is thus closer to the operator of the device than the distal end of the tube . The tube is configured to store and release the AM-element . The AM-element is stored within an inner space of the tube . The AM- element is to be released from the tube after insertion of the tube into the cavity . The AM-element is to be released from the distal end or from a distal end portion of the tube . The release of the AM-element is mediated by the release element . The release element is slidably supported with respect to the tube along a longitudinal axis of the tube . By releasing the AM-element from the tube , the AM-element is deposited at its target position within the cavity .

The AM-element is intended to be stored in the tube in an essentially linear form, i . e . in an essentially straight manner, with preferably no angles . The AM-element is intended to be bent only once , namely when the AM-element is released from the tube . This will be further described in connection with the description of the deflection means below .

The AM-element may not extend along the entire longitudinal extension of the inner space of the tube . The tube may have a longitudinal extension that is considerably larger compared to the longitudinal extension of the AM-element . In this case , the AM-element is preferably stored towards the distal end or in the distal end portion of the tube since the AM-element is to be released from the distal end or from the distal end portion of the tube .

The tube may be essentially straight or the tube may have a slight curvature in a proximal end portion of the tube . In case the tube has the curvature and the AM-element is intended to be positioned in the upper portion of the nasal cavity for collecting nasal secretion located in the ol factory cleft , the curvature of the tube is configured to point towards the septum ( i . e . medial ) during the insertion of the tube into the nasal cavity . In other words , the proximal end portion of the tube is bent towards the septum . Such a curvature facilitates the correct positioning of the tube , in particular by facilitating the correct insertion route of the tube along the septum towards the upper portion of the nasal cavity . Speci fically, the curvature avoids deviating from the correct insertion route in a lateral direction . In case the tube has the curvature in the proximal end portion of the tube , an angle p between the septum and the proximal end of the tube preferably is smaller than or equal to 45 ° , more preferred smaller than or equal to 30 ° , further preferred between 5 ° and 30 ° , further preferred around 20 ° . It is preferred that the curvature in the proximal end portion of the tube does not cause the AM-element stored within the tube to be bent within the tube . This can be achieved, for example , by storing the AM-element in the distal end portion of the tube .

The application device allows positioning the AM-element within the cavity in a targeted and standardi zed manner, which in turn allows collecting the secretion in a standardi zed manner . This signi ficantly decreases variability in the type and amount of secretion that i s absorbed by the AM-element when compared to other means of positioning the AM-element such as a positioning by hand .

The design of the application device allows positioning the AM- element within the cavity in a targeted, easy and reliable manner . In line with this , the design of the application device minimi zes the risk of inj ury of the patient during the positioning of the AM-element , thereby increasing the comfort of the patient and reducing variations in the collected secretion that are caused by inj ury or irritation ( such as traces of tissue or blood) . This is particularly relevant in case the AM-element is to be positioned in the ol factory cleft , i . e . in general above the lower and the middle nasal turbinates , lateral to the upper nasal turbinates and below the ol factory fibers , since this region is very sensitive . In some patients , the anatomy of the nasal cavity is such that the ol factory cleft is located lateral to the middle nasal turbinates rather than lateral to the upper nasal turbinates .

The ef fect of reliably positioning the AM-element while minimizing the risk of inj ury is primarily achieved by causing the AM-element to be bent and/or deflected upon release of the AM- element from the tube . The deflection means is capable to bend the AM-element away from the longitudinal axis of the tube when the AM-element i s released from the tube . The AM-element thus has a curvature during its release from the tube . The deflection means is capable to bend the AM-element away from the longitudinal axis of the tube in a predefined direction . This ensures that the AM-element will be positioned at its intended target position within the cavity . By causing the AM-element to be bent and/or deflected upon release of the AM-element from the tube, contact and contact forces between the AM-element and a mucosa ( or other anatomical boundary) lining the cavity are minimi zed . This increases the safety and comfort of the patient .

Each element which causes the AM-element to be bent and/or deflected upon release of the AM-element from the tube can be regarded as a deflection means . The bending or deflection leads to a release direction of the AM-element that is inclined with respect to the longitudinal axis o f the tube by an angle a > 0 ° .

In case the AM-element is to be positioned in the upper portion of the nasal cavity for collecting nasal secretion located in the ol factory cleft , the deflection means cause the AM-element to bend in a cranial and medial anatomical direction .

The deflection means render the application device suitable for positioning the AM-element in the upper portion of the nasal cavity for collecting nasal secretion located in the ol factory cleft . To reach this region of the nasal cavity, an AM-element that is not caused to be bent during its release , i . e . that is released in a straight or linear form in line with the longitudinal axis of the tube , would not be suitable . Due to the anatomy of the nasal cavity, a straight form upon release may be suitable for reaching the lower portion or the middle portion of the nasal cavity, but not for reaching the upper portion in which the olfactory cleft is located .

Depending on the degree of deflection of the AM-element upon release of the AM-element , the application device of the invention may also be used for positioning the AM-element in the lower portion or the middle portion of the nasal cavity .

The release direction of the AM-element is inclined with respect to the longitudinal axis of the tube by the angle a > 0 ° . The angle a is predefined by the design of the application device , in particular by the design of the deflection means .

For positioning the AM-element in the upper portion of the nasal cavity, the angle a preferably is > 0 ° and smaller than or equal to 50 ° , preferably between 20 ° and 45 ° .

For positioning the AM-element in the middle portion of the nasal cavity, the angle a preferably is > 0 ° and smaller than or equal to 30 ° , preferably between 5 ° and 15 ° . For positioning the AM-element in the lower portion of the nasal cavity, the angle a preferably is > 0 ° and smaller than or equal to 20 ° .

Depending on the individual anatomy of the nasal cavity of a given patient , the operator of the application device may adapt the positioning of the tube to ensure that the AM-element reaches its intended target position upon its release from the tube . For example , the operator may choose a steeper degree of insertion of the tube into the cavity .

For easily adapting the positioning of the tube according to the individual anatomy of the patient , the distal end portion of the tube can preferably be easily bent .

Due to the deflection means , the distal end or the distal end portion of the tube does not need to be positioned such that it directly points to the intended target position of the AM-element . It is rather possible to halt the insertion of the tube before directly pointing to the target position at a location and a distance that is suitable for positioning the AM-element at the target position upon its release from the tube .

Regarding the safety of the patient , in addition to the deflection means , the risk of inj ury is preferably further reduced by at least one stoppage land protruding from the outer circumferential surface of the tube . The stoppage land limits the insertion depth of the tube in the cavity . Another preferred measure for minimi zing the risk of inj ury is to choose a material of the tube in such a manner that the deformability of the tube at its distal end is higher when compared to the deformability at its proximal end . The distal end of the tube is preferably made of a soft material that is bendable in a rubberlike manner . These optional measures are particularly useful for minimi zing the risk of inj ury of the base of the skull in case the AM-element is to be positioned in the ol factory cleft .

A further advantage of the application device is that the secretion that is absorbed by the AM-element after its release from the tube is exclusively absorbed from the intended target position of the AM-element and is thus very pure . The AM-element is protected from any unintended contacts within the cavity by being stored in the tube during its insertion into the cavity and along the entire route for reaching the location that is suitable for pos itioning the AM-element at the intended target position, for example in the upper part of the nasal cavity . Therefore , there is no risk of contamination of the absorptive matrix of the AM-element by secretion or fluids absorbed during insertion of the AM-element into the cavity . Likewise , there is no risk of dilution of the secretion or of bacterial or dust contamination of the secretion by fluid absorbed during insertion of the AM-element into the cavity, in particular in case the tube is inserted into the nasal cavity through the respective nostril . For example , in case the AM-element is to be positioned in the ol factory cleft by hand rather than by us ing the application device of the invention, contamination and dilution of the target secretion easily occurs by wiping the AM-element along the mucosa in the lower parts of the nasal cavity during its insertion into the nose . Such types of contamination and dilution are a maj or source of variability in the type and amount of secretion that is absorbed by the AM-element . The extent of such types of contamination and dilution also depends on the experience of the examiner who collects the secretion . Using the application device for positioning the AM-element at its intended target position avoids undesired contamination and dilution of the secretion collected by the AM-element . This results in a much better quality of the secretion collected . It also results in a higher reproducibility of secretion collection . This contributes to collecting the secretion in a standardi zed manner .

Avoiding undesired contamination and dilution of the secretion collected by the AM-element also increases the sensitivity of the subsequent analysis of the secretion .

By having the AM-element protected from any unintended contacts within the cavity by being stored in the tube during its insertion into the cavity, there is also no risk of incomplete absorption of secretion at the intended target position . Such incomplete absorption could be caused by a reduction of the absorptive capacity of the AM-element at the target position due to fluid absorbed during insertion of the AM-element into the cavity .

During the removal of the AM-element from the cavity after the collection of secretion, undesired contamination and dilution of the secretion collected by the AM-element is less likely since the absorptive capacity of the AM-element is strongly reduced at this time .

In a preferred embodiment , the dimensions of the AM-element are selected in a manner such that the absorptive capacity of the AM-element is close to zero after collecting the intended secretion for an intended collection time . This further avoids undesired contamination and dilution of the secretion collected by the AM-element during the removal of the AM-element from the cavity . Due to the usage of the application device for positioning the AM-element at its intended target position, the secretion collected is very pure , non-diluted und well defined . This cannot be achieved by known sampling techniques .

The AM-element i s not attached to any of the tube , the release element and the deflection means . In other words , the AM-element is not attached to any other part of the application device of the invention . This allows leaving the AM-element within the cavity, in particular within the nasal cavity, without the need to leave the storage and release tube within the cavity at the same time . Accordingly, after the release of the AM-element from the tube , the tube can be removed from the cavity while the AM- element remains in the cavity .

In one embodiment , the tube can then be refilled with a second AM-element for further use , for example for positioning the second AM-element in a second nostril of the patient after having positioned a first AM-element in a first nostril of the patient by using the application device .

However, in a preferred embodiment , the application device of the invention is a single-use device . For practical and hygienic reasons , it is preferable that the application device is a single-use product . Accordingly, a first application device is used for positioning a first AM-element in a first nasal cavity through a first nostril of the patient and a second application device is used for positioning a second AM-element in a second nasal cavity through a second nostril of the patient . After collection, the secretion of the first nasal cavity and the secretion of the second nasal cavity may be combined for further analysis . The removal of the tube from the cavity after the AM-element is released and thereby deposited at its target position allows the AM-element to be left in the cavity for a relatively long period of time , for example for 15 minutes , without discomfort for the patient caused by the tube . It thus allows longer collection times compared to a situation in which the tube also needs to remain within the cavity . This is an important advantage of the application device of the invention, in particular in case the AM-element is used for collecting nasal secretion from the olfactory cleft . Given that the amount of nasal secretion is very low, a long collection time , which correspond to a long residence time of the AM-element within the cavity, such as , for example , 15 minutes , may be needed in order to collect a suf ficient amount of nasal secretion for subsequent analysis . Such long collection times can only be achieved by removing the tube from the nasal cavity while the AM-element remains in the cavity . Having the tube in the nose would present a constant danger of the tube causing damage to the scull base and/or bleeding of the nasal mucosa . Further, the tube would cause a permanent irritation of the nose since the nasal cavity is narrow and very sensitive , especially in the region of the ol factory cleft . It would thus not be comfortable for the patient to have the tube in the nose during the whole time of secretion collection . In addition, due to the permanent irritation caused by the tube , the patient would not be able to sit still for the required collection time without anesthesia .

Extended collection times such as , for example , 15 minutes , cannot be achieved by known sampling techniques , in particular in case nasal secretion from the upper portion of the nasal cavity is to be collected . When using the application device of the invention, there is no time limit for the collection period .

Taken together, the application device of the invention has improved characteristics in at least three aspects . The first aspect concerns the positioning of the AM-element and thus the location of the AM-element in the cavity . As described above , the application device allows positioning the AM-element within the cavity in a targeted and standardi zed manner, which in turn allows collecting the secretion in a standardi zed manner . As also described above , the application device is particularly suitable for positioning the AM-element in the upper portion of the nasal cavity, more speci fically in the ol factory cleft . The second aspect concerns the purity of the secretion collected . As described above , the secretion collected is very pure , non-di- luted und well defined . The third aspect concerns the time of secretion collection . As described above , there is no time limit for the collection period .

In a preferred embodiment , a first application device for positioning a first AM-element in a right nasal cavity and a second application device for positioning a second AM-element in a left nasal cavity are provided . The first and the second application device preferably are mirror-inverted to each other . For example , the location of a rotational mark provided on the outer circumferential surface of the tube or visible from the outside of the tube pre ferably is mirror-inverted when comparing the first and the second application device . The rotational mark will be described in more detail further below . In case the tube has the curvature towards the septum in the proximal end portion of the tube , the curvature may also be mirror-inverted when comparing the first and the second application device . The application device can preferably be easily bent and squeezed without causing too strong contact forces in case of contact of the device with a mucosa lining the cavity . This increases the safety and comfort of the patient . Accordingly, the storage and release tube is preferably made from a flexible material that can be easily deformed . For example , the storage and release tube can be made from soft plastic or soft silicone . The material is also selected to be biocompatible and to be generally suitable for medical devices .

In a preferred embodiment , at least in a pre-usage stage of the device , a release opening of the tube is closed by a closure . The release opening is preferably closed by the closure until the release process of the AM-element . The closure additionally protects the AM-element , in particular the distal end of the AM- element , from potential contamination through the release opening . The release opening may be located at the distal end of the tube or in a side wall of the tube at a distal end portion of the tube .

In a preferred embodiment , the closure and at least a distal end portion of the AM-element are configured to open the closure by pushing forward the distal end of the AM-element when the AM- element is released from tube . The closure protects the AM- element from potential contamination until the release of the AM-element from the tube . In particular, the closure protects the AM-element from potential contamination during its insertion into the cavity .

The closure may be a film attached to the release opening . The film may be attached, for example , by gluing or welding . The film, for example a thin plastic sheet , needs to stay connected to the tube after the AM-element has been pushed through the film so that the film does not get lost within the cavity .

In a preferred embodiment , the closure is a tongue closure comprising a plurality of tongues . The plurality of tongues may form a tapered tip . The advantage of closing the release opening of the tube by a tongue closure is that the tongue closure can be opened by a pushing force applied to the AM-element only . This is due to a bendability of the tongues of the tongue closure . No additional opening means for opening the closure are necessary .

In a preferred embodiment , the application device further comprises at least one shroud means located within a transition region of the tube and the deflection means . The transition region is the region in which a contact between the storage and release tube and the nasal mucosa is most likely . The provision of the shroud means further minimi zes contact and/or contact forces between the storage and release tube and the anatomical boundaries of the cavity, such as contact and/or contact forces between the tube and the nasal mucosa of the nasal cavity . The shroud means also serves as a damper means to soften a possible contact between the storage and release tube and the nasal mucosa . This further increases the comfort of the patients during the insertion of the device and the release of the AM-element and, more importantly, decreases the risk of inj ury of the patient . Likewise , it further increases the acceptance of the procedure by the patients .

In a preferred embodiment , the shroud means comprises at least one cushion element arranged along an outer circumferential surface of the transition region . The shroud means preferably comprises a plurality of cushion elements arranged along the outer circumferential surface of the transition region . By forming the shroud means of one or a plurality of cushion elements , which are arranged along the outer circumferential surface of the transition region, softening a possible contact between the storage and release tube and the nasal mucosa can be achieved .

The cushion element may be formed as a ring-like element surrounding the outer circumferential surface . Alternatively, a plurality of single cushion elements are arranged along the outer circumferential surface . The cushion element ( s ) is/are arranged at positions of the application device that may contact the anatomical boundaries of the cavity such as the nasal mucosa of the nasal cavity . A ring-like element or a ring-like arrangement of the plurality of cushion elements is preferred since it allows using the application device for both the left and the right nasal cavity .

In a preferred embodiment , the storage and release tube is at least partially coated with a soft material that serves as the damper means to soften a possible contact between the storage and release tube and the nasal mucosa .

In a preferred embodiment , the application device further comprises a spreader device having spreading arm means , wherein the application device is attachable to one of the spreading arm means and can thereby be arranged in a gap between the spreading arm means . The spreading arm means are preferably connected with or mounted to the application device . The spreading arm means ensure a suf ficient gaping of the opening of the cavity . This facilitates the insertion of the application device into the cavity . This is particularly relevant for the nasal cavity which is often rather narrow . The spreading arm means may be configured to open the nose in a vertical direction by pushing the nostril upward .

In a preferred embodiment , a scale of marks is provided on the tube along a longitudinal direction of the tube , the scale of marks indicating the insertion depth of the tube into the cavity of the human or animal body . The scale of marks is located within a suitable region of the storage and release tube . When compared with a visible boundary or edge of the cavity of interest , the scale of marks provides an information about the insertion depth of the application device in the cavity . Thus , the operator can easily detect the insertion depth of the tube . In this way, the tube can be easily and controllably inserted at an insertion depth suitable for positioning the AM-element . The controlled insertion also decreases the risk of inj ury of the patient .

In a preferred embodiment , at least one stoppage land protruding from the outer circumferential surface of the tube is provided, wherein the stoppage land is a ring land or at least one platelike land protruding from the outer circumferential surface in the vicinity of the proximal end of the tube . The stoppage land limits the insertion depth of the tube in the cavity, thereby decreasing the risk of inj ury of the patient .

In a preferred embodiment , the at least one stoppage land is arranged proximal of the scale of marks .

In a preferred embodiment , the deflection means is formed by an extended wall portion of the tube protruding from the distal end of the tube and inclined with respect to the longitudinal axis towards the longitudinal axis . The extended wall portion of the tube thus protrudes from one side of the distal end of the tube and bends inward towards the longitudinal axis of the tube . The extended wall portion can also be referred to as an extended end wall portion . The extended wall portion is a simple way of forming and providing the deflection means . It can also be easily integrated into the tube during the production of the tube . The extended wall portion is preferably formed integrally with the storage and release tube so that there is no ris k of loosening of the deflection means within the cavity .

In a further preferred embodiment , the extended wall portion is inclined with respect to the longitudinal axis towards and past the longitudinal axis of the tube . In other words , the extended wall portion bends inward towards and past the longitudinal axis of the tube .

In a preferred embodiment , a tongue closure comprising a plurality of tongues forming a tapered distal tip is provided and is constituting the distal end of the tube . The advantage of closing the distal end of the tube by a tongue closure is that the tongue closure can be opened by a pushing force applied to the AM-element only . This is due to a bendability of the tongues of the tongue closure . No additional opening means for opening the closure are necessary . Closing the distal end of the tube also protects the AM-element from potential contamination until its release from the tube .

In a preferred embodiment , at least one of the tongues of the tongue closure , when compared with at least one other tongue , has an increased bending sti f fness away from the longitudinal axis thereby forming the deflection means . The tongue closure allows integrating the deflection means within the closure by including at least one tongue that is less flexible than the other tongues , i . e . by including at least one tongue that has an increased bending sti f fness when compared to the other tongues . This will lead to deflection of the AM-element during release of the AM-element through the opening at the distal end of the tube .

In another preferred embodiment , the deflection means is a release opening located in a side wall of the tube at a distal end portion of the tube . The release opening is configured to be passed by the AM-element during the release process of the AM- element . Since the release opening is located in the side wall of the tube , pushing the AM-element through the release opening will cause the AM-element to bend and therefore to deflect from the longitudinal axis of the tube . Since the release opening is located in a side wall of the tube , the distal end of the tube is closed . This reduces the risk of undesired contamination of the AM-element until its release from the tube . In this embodiment , the tube preferably has a rounded distal end, which reduces the risk of inj ury of the patient during the insertion of the tube into the cavity . It is further preferred that the tube is tapered towards its distal end, which enables easier and more accurate access into the patient ' s ol factory cleft .

The release opening is preferably closed until the AM-element is to be released . This protects the AM-element from potential contamination until its release from the tube .

The release opening is preferably closed by a tongue closure comprising a plurality of tongues . The advantage of closing the release opening of the tube by a tongue closure is that the tongue closure can be opened by a pushing force applied to the AM-element only . This is due to a bendability of the tongues of the tongue closure . No additional opening means for opening the closure are necessary . Closing the release opening of the tube also protects the AM-element from potential contamination until its release from the tube . In another embodiment , the release opening is a through hole located in the side wall of the tube at the distal end portion of the tube .

In a preferred embodiment , the release element is a piston type rod having a piston portion that is slidably mounted within the tube . Forming the release element as a piston type rod allows pushing and releasing the AM-element .

In another preferred embodiment , the release element is a push rod reaching through a longitudinal side slot of the tube and the push rod being slidably movable along the slot . By moving the push rod along the slot , the AM-element is moved forward and push forces are applied to the AM-element . This leads to the release of the AM-element . Since there is no risk of kinking of the AM-element upon using the push rod, a soft AM-element can be used .

In a preferred embodiment , the AM-element has a ribbon or leash at its proximal end for removing the AM-element from its target position within the cavity . The ribbon or leash facilitates the removal of the AM-element from the cavity . Of advantage , the removal can be performed without contacting the absorptive matrix itsel f . The ribbon or leash renders the removal process smooth and easy, thereby increasing the comfort of the patient .

The ribbon or leash may be attached to the AM-element at the proximal end of the AM-element or in a region of the proximal end of the AM-element .

In one embodiment , the ribbon or leash unfolds by removing the tube from the cavity after the release of the AM-element . The ribbon or leash unfolds along a removal route of the tube . This ensures that the ribbon or leash will extend to the entrance of the cavity so that the AM-element can be removed from the cavity by pulling at the ribbon or leash . The removal route of the tube will generally be the same route as used for its insertion into the cavity .

In another embodiment , the ribbon or leash passes from the AM- element out through the release opening and then essentially along the outside of the tube towards the proximal end of the tube . The ribbon or leash extends beyond the proximal end of the tube and is preferably held by a notch located on the outer surface of the application device in a region of the proximal end of the application device . In this embodiment , when the AM- element is released from the tube , the ribbon or leash is already outside the tube and does not need to pass through the tube as the tube is removed from the cavity .

In a preferred embodiment , a radiopaque marker is attached to the AM-element , for example in the form of a stripe or a dot . The radiopaque marker facilitates detecting the AM-element in case it gets lost within the cavity .

In a preferred embodiment , the application device comprises an illuminating means capable to illuminate the cavity of the human or animal body during an insertion process of the application device and/or during a release process of the AM-element . I lluminating the cavity facilitates a correct and reproducible positioning of the application device as well as monitoring the release of the AM-element . In this way, the illuminating means also facilitates a correct and reproducible positioning of the AM-element at its intended target position within the cavity . In a preferred embodiment , at least the tube is tapered along at least a portion of its longitudinal dimension, wherein the tapering tapers towards the distal end of the tube . Accordingly, the circumference of the proximal end of the tube i s larger than the circumference of the distal end of the tube . Tapering the tube and/or the complete application device towards its distal end facilitates the insertion process of the application device into the cavity, especially in case the cavity is narrow . For example , in case the AM-element is to be positioned in the region of the ol factory cleft within the nasal cavity, the anatomical surrounding that needs to be passed by the application device gets narrower towards the target position of the AM-element . Tapering at least the tube towards its distal end facilitates finding an appropriate route of inserting the device while minimi zing the risk of inj ury of the patient and increasing the patient ' s comfort .

In a preferred embodiment , a latch mechanism is provided at a latching position, where the AM-element is released capable of latching the release element . The latch mechanism provides a haptic and acoustic feedback to the operator of the application device , the feedback indicating that the AM-element is completely released . This increases the safety of the patient , in particular in case the operator cannot visually confirm the complete release of the AM-element . In case the release element is a piston type rod having a piston portion that is slidably mounted within the tube , the AM-element preferably is released capable of latching the piston portion of the release element . Upon complete release of the AM-element , the piston portion may, for example , engage in a recess arranged within the tube , the engagement being accompanied by an acoustic signal such as a click sound ( acoustic feedback to the operator ) . After engaging in the recess , the piston portion can no longer be moved (haptic feedback to the operator ) . This also ensures that the pushing portion cannot be moved further forward, thereby increasing the safety of the patient .

In a preferred embodiment , on an inner circumferential surface of the tube a screw thread protruding from the inner circumferential surface i s provided, the screw thread being capable of acting together with the AM-element in order to rotate the AM- element along its longitudinal axis during the release process of the AM-element . The internal screw thread or, alternatively, internal snakeli ke wound ribs cause the AM-element to rotate around its longitudinal axis during its release from the tube , thereby facilitating the positioning of the AM-element by a drill-like rotation of the AM-element . The rotation of the AM- element facilitates the positioning of the AM-element at its intended target position .

In a preferred embodiment , a first portion of the tube has an increased deformability when compared to a second portion of the tube , wherein the first portion is intended to be inserted into the cavity and the second portion is not intended to be inserted into the cavity . In line with that , in a preferred embodiment , the deformability of the tube at its distal end is higher when compared to the deformability at its proximal end . The di f ferent levels of deformability render the insertion process of the application device more comfortable for both the operator of the device and the patient .

In a preferred embodiment , wing-like plate elements protrude from the outer circumferential surface of the tube in a region of the proximal end of the tube . The plate elements allow the operator to dose/meter the application force applied to the release element in a more sensitive manner . In this way, the speed of the release process of the AM-element can be controlled more easily . This decreases the risk of inconvenience of the patient which may be caused by a rapid release of the AM-element .

In case the application device is intended for collecting nasal secretion, the wing-like plate elements preferably have a total dimension that is larger than the entrance of a nostril , so that the wing-like plate elements also serve as stoppage means that limit the insertion depth of the tube in the nasal cavity . This decreases the risk of inj ury of the patient such as an inj ury to the base of the skull .

In a preferred embodiment , one or a plurality of rotational marks are provided on the outer circumferential surface of the tube or visible from the outside of the tube , wherein the at least one rotational mark is located such that when the device is positioned correctly with respect to its rotational axis in the nasal cavity, at least one rotational mark is configured to be directed to the forehead or to the chin or to the nasal septum . The rotational mark allows the operator of the device to control the correct positioning of the device within the nasal cavity before initiating the release process of the AM-element .

In a preferred embodiment , the AM-element has an elongated shape .

In a preferred embodiment , the AM-element has a circular, oval or a polygonal cross-sectional shape having rounded edges in case of a polygonal cross-sectional shape . A polygonal cross- sectional shape having rounded edges decreases the risk of rolling-up of the AM-element . It also decreases the ris k of any kind of inj ury during the release of the AM-element from the tube and/or during absorption . A preferred polygonal cross-sectional shape having rounded edges is a triangular cross-sectional shape having rounded edges .

In case the tube is provided with the screw thread protruding from the inner circumferential surface of the tube , a AM-element having a polygonal cross-sectional shape is preferred since it can be gripped more easily by the screw thread . This increases the reliability of the rotation of the AM-element during the release process of the AM-element .

In a preferred embodiment , the AM-element has a constant cross- sectional shape along its longitudinal axis or is tapered towards the distal end of the AM-element . In both cases , the distal end and the proximal end of the AM-element are preferably rounded . The tapered design of the distal end of the AM-element facilitates the positioning of the AM-element at its intended target position . It also decreases the risk of any kind of inj ury during the release of the AM-element from the tube .

In a preferred embodiment , the AM-element is made of an elastic and compressible material . The material may be a fibrous matrix . The material needs to be suitable for collecting secretion for subsequent analysis . Examples of suitable materials comprise nylon flocked, cellulose , cotton, a natural sponge material ( such as a sponge material made from natural cellulose ) , a synthetic sponge material ( such as a sponge material made from polyvinyl alcohol , hydroxylated polyvinyl acetate and/or polyurethane ) and/or a foam material ( such as a foam material made from fiber polymers ) .

In a preferred embodiment , the AM-element has a length of about 2 cm to 6 cm, preferably about 3 cm to 6 cm, further preferred about 4 cm to 6 cm, and a diameter of about 2 mm to 6 mm, preferably about 2 mm . As described above , the AM-element is preferably tapered towards its distal end . In this case , the smallest diameter of the AM-element , at a distal end portion of the AM-element , is preferably about 2 mm, and the largest diameter of the AM-element , at a proximal end portion of the AM- element , is preferably about 3 mm to 6 mm .

In a preferred embodiment , the AM-element is at least partially sti f fened against kinking . To this end, the AM-element preferably has a spine element at least along a portion of its longitudinal dimension, wherein the spine element is bendable but not kinkable within the estimated range of push forces applied to the AM-element during its release from the tube . The spine element increases the kinking sti f fness of the AM-element while maintaining the bendability of the AM-element along its extension direction . In this way, the spine element allows to define the movement of the AM-element during the release process more accurately and to render it more predictable . The spine element is further helpful with respect to avoiding an agglomeration or lumping or rolling-up of the AM-element within the storage and release tube caused by pushing forces applied to the AM-element during the release process . This facilitates the positioning of the AM-element at its target position in a stretched form . The spine element can be arranged, for example , within the AM-element ( such as at the center of the AM-element ) or on one or more sides of the AM-element . In case of a polygonal AM-element , the spine element can be arranged, for example , on each polygonal side .

Alternatively, the AM-element may be sti f fened against kinking due to the sti f fness of the material it is made of . As described above , the material preferably is a compressible material and the sti f fness can be caused by the compression of the material . In a preferred embodiment , the compressible material of the AM- element decompresses upon contact with fluid such as biological secretion, thereby increasing its dimensions and decreasing its sti f fness . The decreased sti f fness increases the comfort of the patient during the collection time and during the removal process of the AM-element .

In a preferred embodiment , as an additional safeguard for the patient , the AM-element is surrounded by a thin stretchable mesh or layer for preventing residues of the AM-element from remaining in the cavity upon removal of the AM-element from the cavity . The mesh or layer ensures the physical integrity of the AM- element . The mesh or layer is designed so that it does not signi ficantly reduce the absorptive surface of the AM-element .

In a second aspect , the present invention relates to a collection kit applicable for collecting biological secretion from a cavity of a human or animal body, the collection kit comprising the application device of the invention, wherein the collection kit further comprises a vessel arrangement for storing the AM-element after the collection of secretion .

The collection kit preferably is applicable for col lecting nasal secretion, in particular nasal secretion from a region above the nasal turbinates , in particular nasal secretion from the ol factory cleft .

The vessel arrangement preferably is configured to fit into a centri fugal device for centri fugation of the AM-element . Centri fugation of the AM-element is an ef ficient way of recovering the secretion from the AM-element for further analysis . Providing a vessel arrangement configured to fit into a centri fugal device eliminates a trans fer step for trans ferring the AM-ele- ment from a transport vessel to a centri fuge vessel . Elimination of the trans fer step saves time and material and reduces the risk of contamination of the sample . It also reduces the risk of loss of sampling material .

In a preferred embodiment , the vessel arrangement comprises an inner vessel and an outer vessel , wherein the inner vessel is configured to receive the AM-element after collection of secretion and has at least one opening such that an inner space of the inner vessel communicates with an inner space of the outer vessel . The inner vessel has a smaller diameter compared to the outer vessel and has a shorter length compared to the outer vessel , such that the inner vessel is completely insertable within the outer vessel . Such a two-shell design of the vessel arrangement having an inner vessel ( smaller vessel ) and an outer vessel ( larger vessel ) facilitates the further processing of the AM-element by centri fugation . The outer vessel is configured to fit into a centri fugal device . During centri fugation, the AM- element remains in the inner space of the inner vessel while the secretion separates from the AM-element and locates to the inner space of the outer vessel .

In a preferred embodiment , the inner vessel is insertable into and lockable with respect to the outer vessel of the vessel arrangement . In this way, a defined mechanical fixation of the inner vessel in relation to the outer vessel is ensured .

Nasal secretion i s preferably collected from each the left nasal cavity and the right nasal cavity of a subj ect . After collection, the secretion of the left nasal cavity and the secretion of the right nasal cavity preferably is combined for further analysis . To this end, the vessel arrangement preferably is configured to store two AM-elements in the same vessel after the collection of secretion . In this case , the secretion collected by the two AM- elements will be easily combined during the recovery of the secretion from the two AM-elements , for example by centri fugation . In this case , it is not necessary to trans fer any of the secretion from one vessel to another vessel in order to combine the secretion of the two AM-elements . In this way, a potential loss of the secretion sample is prevented .

The vessels of the vessel arrangement are generally made of a material that is low-binding and/or non-adsorptive with respect to proteins and other analytes . The vessels can be made of , for example , glass , polyethylene or polypropylene .

In a third aspect , the present invention relates to the use of the application device of the invention or the collection kit of the invention for collecting nasal secretion, in particular for collecting nasal secretion from the ol factory cleft .

The present disclosure further relates to an application device applicable for positioning an absorptive matrix (AM) -element within a cavity of a human or animal body, the application device comprising :

- a storage and release tube having a distal end and a proximal end, the tube is configured to store and release an AM- element ;

- a release element being slidably supported with respect to the tube along a longitudinal axis of the tube , the release element is configured to release the AM-element from the tube ;

- at least one deflection means arranged at the distal end of the tube , the deflection means is capable to bend the AM- element away from the longitudinal axis of the tube when the AM-element is released from the tube .

The application device of the disclosure can be filled with an AM-element . The AM-element is to be positioned within an inner space of the tube .

The features , advantages and preferred embodiments of the application device of the invention as described above likewise apply to the application device of the disclosure .

The present disclosure further relates to an application kit applicable for positioning an AM-element within a cavity of a human or animal body, the application kit comprising the application device of the disclosure and at least one AM-element configured to be insertable into an inner space of the tube .

The present disclosure further relates to a collection kit applicable for collecting biological secretion from a cavity of a human or animal body comprising the application device of the disclosure , wherein the collection kit further comprises a vessel arrangement for storing the AM-element after the collection of secretion .

In a third aspect , the present disclosure relates to the use of the application device of the disclosure or the collection kit of the disclosure for collecting nasal secretion .

The features , advantages and preferred embodiments of the collection kit of the invention as described above likewise apply to the collection kit of the disclosure . The same applies to the use of the application device of the disclosure or the collection kit of the disclosure . The invention will now be described in further detail with reference to the accompanying drawings .

Fig. 1 shows a perspective view of a human nose 1. The perspective view is shown in a semitransparent manner. The human nose 1 has a left nostril 2 and a right nostril 3. Corresponding to the nostrils 2, 3 there is a left nasal cavity 4 and a right nasal cavity 5. The nasal cavities 4, 5 are separated by a nasal septum 6. The nasal cavities 4, 5 are covered by mucosa. At an upper end of the nasal cavities 4, 5 there is a scull base 7, which separates the nasal cavities 4, 5 from an inner space of the scull. In some parts, the scull base 7 is made of very thin bone material and has a porous structure, having a plurality of through holes 8, through which olfactory fibers (not shown) protrude into the upper region of the nasal cavities 4, 5.

Three nasal turbinates 9 are located opposite to the nasal septum 6 in each nasal cavity 4, 5. For collecting nasal secretion from an olfactory cleft that is located in the vicinity of the scull base 7, an absorptive matrix (AM) -element 10 is positioned in the olfactory cleft, i.e. in the narrow space between the scull base 7, the nasal septum 6 and the uppermost nasal turbinate 9, medial the uppermost nasal turbinate 9 and just below the scull base 7. This region of the nasal cavity 4, 5 is difficult to reach from the nostrils 2, 3 by an instrument or a device. The AM-element 10 has been positioned in the olfactory cleft by release from an application device of the invention (not shown in Fig. 1) . The application device has already been removed.

Given that the amount of nasal secretion is very low, a long collection time, which correspond to a long residence time of the AM-element 10 below the scull base 7, such as, for example, 15 minutes , is needed in order to collect a suf ficient amount of nasal secretion for subsequent analysis .

The AM-element 10 has a distal end 11 and a proximal end 12 . A leash 13 is attached to the proximal end 12 . The leash 13 allows pulling out the AM-element 10 from its absorption position shown in Fig . 1 to remove the AM-element 10 from the nasal cavity 4 , 5, passing the respective nostril 2 , 3 .

In the position of the AM-element 10 shown in Fig . 1 , the distal portion of the AM-element 10 is essentially arranged in a horizontal direction below the scull base 7 and the proximal portion of the AM-element 10 bends to a nearly vertical direction with respect to its distal portion . The proximal portion of the AM- element 10 is located such that the proximal end 12 is essentially directed towards the respective nostril 2 , 3 .

Several embodiments of an application device of the invention will now be described with reference to Fig . 2A, 2B, 3A, 3B and 4 .

A first embodiment of the application device of the invention will now be described with respect to Figs . 2A, 2B .

An application device 100 according to the invention has a storage and release tube 101 , which is configured to store an AM- element 10 within an inner space 102 of the storage and release tube 101 . The storage and release tube 101 has a distal end 103 and a proximal end 104 . The storage and release tube 101 has a longitudinal axis L .

At the distal end 103 , the storage and release tube 101 has a release opening 105 , through which the AM-element 10 can be pushed out of the inner space 102 by a release element 106 . The release element 106 is a piston type rod and has a piston portion 107 and a push rod portion 108 . The piston portion 107 is slidably mounted within the inner space 102 of the storage and release tube 101 . The piston portion 107 is configured to be able to act against the proximal end 12 of the AM-element 10 , thereby pushing the AM-element 10 through the release opening 105 out of the storage and release tube 101 . For example , the piston portion 107 is configured to trans fer pressure on the AM-element 10 , thereby pushing the AM-element 10 through the release opening 105 out of the storage and release tube 101 .

The storage and release tube 101 can have guiding means for guiding and stabili zing at least a portion of the piston portion 107 and/or the push rod portion 108 . The guiding means may be formed, for example , by a wall portion of the storage and release tube 101 that has an increased wall thickness or by guiding rails protruding from an inner surface of the tube 101 .

The application device 100 has a latch mechanism that provides haptic feedback to the operator of the application device 100 upon complete release of the AM-element 10 . For example , the storage and release tube 101 has a snap-in point (not shown) into which the release element 106 snaps when the piston portion 107 has completely pushed the AM-element 10 out of the release opening 105 . The latch mechanism may additionally provide acoustic feedback to the operator of the application device 100 upon complete release of the AM-element 10 .

The application device 100 further has a safety mechanism (not shown) that prevents an unintended movement of the release element 106 before the release of the AM-element 10 is intended by the operator of the application device 100 , for example during transport or unpacking of the application device 100 .

The piston portion 107 is provided with an opening (not shown) to enable the leash 13 to pass through the opening from the inner space 102 of the storage and release tube 101 to the outside thereof .

The release opening 105 is located in the region of the distal end 103 of the storage and release tube 101 . A wall portion 109 is protruding from the distal end 103 of the storage and release tube 101 . The wall portion 109 is formed in a manner such that it is inclined with respect to the longitudinal axis L towards the longitudinal axis L . In this way, the wall portion 109 acts as a deflection means 110 , which causes the AM-element 10 to bend away from the longitudinal axis L of the storage and release tube 101 when the AM-element 10 is released from the storage and release tube 101 .

The AM-element 10 is not attached to any of the tube 101 , the release element 106 and the deflection means 110 .

The application device 100 with the AM-element 10 being stored within the storage and release tube 101 can be inserted into one of the nasal cavities 4 , 5 . During insertion, the storage and release tube 101 does not necessarily need to be bent . When inserting the storage and release tube 101 far enough into one of the nasal cavities 4 , 5 in a protruding movement , the AM- element 10 can be released to the desired target region below the scull base 7 and medial the uppermost nasal turbinate 9 by bending of the AM-element 10 . The AM-element 10 is caused to bent by the deflection means 110 , which is a protruding ( extended) wall portion 109 that bends the AM-element 10 during its release through the release opening 105 .

In order to avoid inj uries of the mucosa, a shroud means 112 is provided in a transition region 111 of the storage and release tube 101 close to its distal end 103 . The shroud means 112 comprises at least two cushion elements 113 , which are arranged along an outer circumferential surface 114 of the storage and release tube 101 , more speci fically along the outer circumferential surface of the transition region 111 .

Further, a scale of marks 115 is provided on the outer circumferential surface 114 of the storage and release tube 101 or at least visible from the outside of the storage and release tube 101 . The scale of marks 115 is provided along a longitudinal direction 20 of the storage and release tube 101 . The scale of marks 115 indicates the insertion depth of the storage and release tube 101 into the cavity of the human or animal body, in particular into the nasal cavity 4 , 5 . The scale of marks 115 helps the operator of the application device to estimate the position of the distal end 103 of the storage and release tube 101 for releasing the AM-element 10 to the intended target position .

Further, at least one plate element 116 may be provided at the proximal end 104 of the storage and release tube 101 . The plate element 116 protrudes radially outward from the outer circumferential surface 114 and acts as a support means for the operator for a better holding of the application device 100 during the positioning of the AM-element 10 . Two oppositely protruding plate elements 116 can be formed as a finger flange to be grasped by two fingers of the operator of the application device 100 so that the operator can press in the push rod portion 108 with the operator' s thumb .

Additionally, at least one rotational mark 117 may be provided on the outer circumferential surface of the storage and release tube 101 or at least visible from the outside of the storage and release tube 101 in order to facilitate the correct positioning of the storage and release tube 101 within the nasal cavity 4 , 5 along a rotational direction 21 . The rotational mark 117 may be positioned such that it corresponds to the opening direction of the release opening 105 and/or such that it is directed to the chin and/or the nasal septum 6 or another characteristic portion of the human or animal body, which helps to position the application device 100 correctly with respect to the intended target position of the AM-element 10 .

Further, an illuminating device 118 may be integrated at a suitable position of the application device 100 in order to illuminate the cavity of the human or animal body, in particular the nasal cavity 4 , 5 , during the release process of the AM-element 10 . I lluminating the cavity further facilitates the correct positioning of the AM-element 10 at its intended target position .

In Fig . 2A, the application device 100 is shown in a state in which the AM-element 10 is in its storage ( retracted) position before its release and in a state in which the AM-element 10 is in a partially released position, i . e . in which the AM-element 10 has partially left the storage and release tube 101 and therefore passes through the release opening 105 . In a state before the release of the AM-element 10 , it is preferred that the release opening 105 is closed by a closure (not shown in Fig . 2A) to avoid undesired contamination of the AM-element 10 through the release opening 105 during its insertion into the cavity . The closure may be a film (not shown) attached to the release opening 105 by gluing or welding . It is preferred to configure the closure such that it can be pushed away or pushed open by the distal end 11 of the AM-element 10 when the AM- element 10 is pushed out of the storage and release tube 101 . The film, for example a thin plastic sheet , needs to stay connected to the storage and release tube 101 after the AM-element 10 has been pushed through the film so that the film does not get lost within the cavity .

Further, the storage and release tube 101 may be formed of a material which is sti f fer when going to the proximal end 104 and which is more f lexible/def ormable when going to the distal end 103 in order to minimi ze inj uries of the mucosa . The deflection means 110 , however, is of an increased sti f fness , at least against bendability, in order to ensure the desired deflection of the AM-element 10 away from the longitudinal axis L when the AM-element 10 is released from the tube 101 . To this end, the deflection means 110 may be formed with an increased wall thickness , which increases the sti f fness against bending . Additionally or alternatively, it is possible to design the deflection means 110 in a bowl shape , which is a shape that comprises a higher sti f fness against bending when compared to a flat plane design and is thus suitable to act as a deflection means 110 .

Another alternative is to form the deflection means 110 of a material , which has an increased sti f fness/resistance against bending when compared to the bending sti f fness of the AM-element 10 .

Fig 2B shows an enlarged view of detail X shown in Fig . 2A. The deflection means 110 is formed by the extended wall portion 109 of the storage and release tube 101 protruding from the distal end 103 of the storage and release tube 101 and inclined with respect to the longitudinal axis L towards the longitudinal axis L .

It is to be understood that each element which causes a release direction 22 of the AM-element 10 that is inclined with respect to the longitudinal axis L by an angle a > 0 ° can be seen as an deflection means 110 .

A second and third embodiment of the application device 100 of the invention will now be described with reference to Figs . 3A, 3B and 4 . Elements having the same function and/or the same location will be referred to with the same reference numerals . In order to avoid unnecessary replication, the embodiments of Figs . 3A, 3B and 4 will only be described with respect to their dif ferences between the second/ third embodiment and the first embodiment described above . Statements regarding the function and/or the features of the first embodiment described above are also applicable to the embodiments described below and will therefore not be repeated .

In the embodiment shown in Fig . 3A, the closure is formed as a tongue closure 130 , which is composed of a plurality of tongues 131 . The tongues 131 each have a tongue tip 132 , which is directed towards the distal end 103 of the storage and release tube 101 . The plurality of tongue tips 132 constitute the distal end 103 of the storage and release tube 101 . At least one of the tongues 131 is designed with a higher bending sti f fness to and from the longitudinal axis L of the storage and release tube 101 when compared with the bending sti f fness to and from the longitudinal axis L of the storage and release tube 101 of the other tongues 131 . The one or more tongues 131 that have a higher bending stiffness form the deflection element 110 as these tongues 131 of higher bending stiffness are not bent away or bent away to a smaller extent by the protruding AM-element 10 during its release. In other words, the softer tongues 131, i.e. the tongues 131 having a lower bending stiffness, are bent away from the longitudinal axis L when the AM-element 10 is pushed through the tongue closure 130 during its release and therefore are of a lower resistance when compared to the stiffer tongues 131. As a result, the AM-element 10 will be released from the storage and release tube 101 through the tongue closure 130 in a release direction 22 that is inclined with respect to the longitudinal axis L by the angle a. The release direction 22 also has the inclination angle (deflection angle) a > 0° with respect to the longitudinal axis L. The degree of deflection can be influenced by selecting the degree of difference between the bending stiffness of the stiffer tongues 131 and the bending stiffness of the softer tongues 131.

A further way of influencing the stiffness of the tongues 131 is based on the length of the tongues 131 along the longitudinal direction 20 of the tongues 131. Shorter tongues 131 usually have a higher bending stiffness compared to longer tongues 131 made of the same material and the same geometry.

Fig 3B shows an enlarged view of detail Y shown in Fig. 3A. The stiffer tongue 131' shown in Fig. 3B forms the deflection means 110 as it is stiffer compared to the other tongues 131, which are bent away by the AM-element 10 protruding trough the tongue closure 130. Because of the difference in stiffness of the tongues 131, 131', the AM-element 10 is bent away from the longitudinal axis L of the storage and release tube 101 when it is released from the storage and release tube 101 by a pushing movement of the piston portion 107 .

A further feature shown in Fig . 3A is that the storage and release tube 101 is designed in a tapered way along its longitudinal dimension, tapering towards the distal end 103 of the tube 101 . This is also applicable to the other embodiments described above or below .

In addition, the embodiment shown in Fig . 3A has a ring land 140 , which serves as a stoppage land . The ring land 140 protrudes from the outer circumferential surface 114 of the storage and release tube 101 in the vicinity of the proximal end 104 . The ring land 140 serves as a stoppage land which limits the insertion depth of the tube 101 in the cavity, avoiding an insertion that would be too far and thereby cause inj uries .

To support the bendability of the AM-element 10 and to avoid unintended kinking of the AM-element 10 , the AM-element 10 may comprise a spine element 15 . The spine element 15 renders the AM-element 10 more rigid against kinking but can easily be bent by the deflection means 110 such that the AM-element 10 can be smoothly pushed out of the storage and release tube 101 .

A third embodiment of the application device 100 of the invention will now be described with reference to Fig . 4 .

The application device 100 of the third embodiment comprises a spreader device 200 having spreading arm means 201 , which are connected to actuation arms 202 . The actuation arms 202 are j oined by a pivot 203 in a manner that when the actuation arms 202 are pushed together, the spreading arm means 201 are moved away from each other thereby spreading an opening into which the spreading arm means 201 are inserted ( e . g . an opening of the nasal cavity 4 , 5 ) .

The embodiment of the application device 100 shown in Fig . 4 further di f fers from the embodiments described above by a longitudinal side s lot 150 extending at least partially over the longitudinal direction 20 of the storage and release tube 101 . Within the longitudinal side slot 150 , a push rod 151 is slidably mounted such that the push rod 151 can be moved along the longitudinal side slot 150 by pushing it forward and backward .

One end of the push rod 151 extends into the inner space 102 of the storage and release tube 101 and acts on the AM-element 10 that is stored within the storage and release tube 101 . By pushing the push rod 151 towards the distal end 103 of the storage and release tube 101 , it is possible to release the AM-element 10 from the storage and release tube 101 .

The additional features described above ( spreader device 200 , push rod 151 acting on the AM-element 10 via a longitudinal side slot 150 ) are also applicable to the embodiments described above .

Fig . 5 shows an embodiment of an AM-element 10 suitable for the application device 100 of the invention . The AM-element 10 has the distal end 11 and the proximal end 12 . The leash 13 is attached to the proximal end 12 . Additionally, the AM-element 10 has the spine element 15 located at the center, thereby representing the longitudinal axis Ls of the AM-element 10 . The AM- element 10 is made of a material that is capable of absorbing secretion such as nasal secretion . In order to sti f fen the material of the AM-element 10 against kinking, the spine element 15 is provided . The spine element 15 has a suf ficient sti f fness against kinking while being suf ficiently bendable by the deflection means 110 of the application device 100 .

The spine element 15 may alternatively be located on one or more sides of the AM-element , for example , in case of a polygonal AM- element , on each polygonal side .

All of the embodiments described above may additionally comprise a screw thread protruding inwards from an inner circumferential surface of the storage and release tube 101 . The screw thread (not shown) may have a spiral-like winding along the inner circumferential surface and may interact with the AM-element 10 during its release , thereby causing the AM-element 10 to rotate in the rotational direction 21 along its longitudinal axis Ls during the release process of the AM-element ( 10 ) . This measure facilitates the release of the AM-element 10 as it acts like a drill during its movement to the desired target position .

Fig . 6 shows a cross sectional view of a vessel arrangement 300 that will now be described . The vessel arrangement 300 is suitable for safely storing and/or transporting an AM-element after the collection of secretion 305 . The vessel arrangement 300 is part of an embodiment of the collection kit of the invention, the collection kit comprising the application device 100 of the invention and the vessel arrangement 300 for storing the AM- element 10 after the collection of secretion 305 .

The vessel arrangement 300 is composed of an outer vessel 301 and an inner vessel 302 . The inner vessel 302 has a smaller diameter compared to the outer vessel 301 and has a shorter length compared to the outer vessel 301 , such that the inner vessel 302 is completely insertable within the outer vessel 301 . After the collection of secretion, the AM-element 10 can be stored within the inner vessel 302 , which preferably has an inner diameter equal to or slightly bigger than the outer diameter of the AM-element 10 .

In a preferred embodiment , the vessel arrangement 300 comprises an inner vessel 302 and an outer vessel 301 , wherein the inner vessel 302 is configured to receive the AM-element 10 after collection of secretion 305 and has at least one opening 303 such that an inner space of the inner vessel 302 communicates with an inner space of the outer vessel 301 . Such a two shell design of the vessel arrangement 300 having an inner vessel ( smaller vessel ) 302 and an outer vessel ( larger vessel ) 301 facilitates the further processing of the AM-element 10 by centri fugation . The outer vessel 301 is configured to fit into a centri fugal device . During centri fugation, the AM-element 10 remains in the inner space of the inner vessel 302 while the secretion 305 separates from the AM-element 10 and locates to the inner space of the outer vessel 301 .

The inner vessel 302 has an opening 303 at its bottom end . The inner vessel 302 is arranged within the outer vessel 301 . Both of the vessels 301 , 302 may be closed by a cap 304 . The outer vessel 301 is preferably configured to fit into a rotating carousel of a centri fugal device . By centri fuging the vessel arrangement 300 comprising the AM-element 10 , the secretion 305 separates from the AM-element 10 and passes through the opening 303 by centri fugal forces . The secretion 305 is thus collected at the bottom of the outer vessel 301 when the vessel arrangement 300 is exposed to centri fugal forces . List of reference signs

Human nose

Left nostril

Right nostril

Left nasal cavity

Right nasal cavity

Nasal septum

Skull base

Through holes

Nasal turbinate

AM-element

Distal end ( of the AM-element )

Proximal end ( of the AM-element )

Leash

Spine element

Longitudinal direction

Rotational direction

Release direction

Application device

Storage and release tube

Inner space

Distal end

Proximal end

Release opening

Release element

Piston portion

Push rod portion

Wall portion

Deflection means

Transition region

Shroud means 113 Cushion element

114 Outer circumferential surface

115 Scale of marks

116 Plate element

117 Rotational mark

118 I lluminating device

130 Tongue closure

131 Tongue

131 ’ Sti f fer tongue

132 Tongue tip

140 Ring land

150 Longitudinal side slot

151 Push rod

200 Spreader device

201 Spreading arm means

202 Actuation arms

203 Pivot

300 Vessel arrangement

301 Outer vessel

302 Inner vessel

303 Opening

304 Cap

305 Secretion

L Longitudinal axis

Ls Longitudinal axis of the AM-element a Angle