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Title:
APPLICATION OF HUMIDITY, OZONE AND SEVERAL CHEMICALS TOGETHER ON FRESH FRUIT AND VEGETABLES
Document Type and Number:
WIPO Patent Application WO/2013/126024
Kind Code:
A1
Abstract:
The invention is related to the application system and implementation method of humidity, ozone, various chemicals and/or different physical applications together on fresh fruit and vegetables.

Inventors:
ILHAN, Kadir (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
SEHIRLI, Sercan (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
KARABULUT, Ozgur (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
Application Number:
TR2013/000063
Publication Date:
August 29, 2013
Filing Date:
February 20, 2013
Export Citation:
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Assignee:
ILHAN, Kadir (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
SEHIRLI, Sercan (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
KARABULUT, Ozgur (Gorukle Kampusu Uludag Universitesi, Ziraat Fakultesi Bitki Koruma Bolumu, Bursa, 16059, TR)
International Classes:
A23L3/3445; A23B7/152; A23B7/154; A23L3/3463
Foreign References:
US20060130498A12006-06-22
US20050161636A12005-07-28
CN101530124A2009-09-16
JP2009171889A2009-08-06
JP2004173904A2004-06-24
JP2007040681A2007-02-15
CN201028914Y2008-02-27
TR200904998U2009-06-26
TR200504202U2005-10-20
TR200708002A
Other References:
MCCLURKIN, J.D.; MAIER, D.E: 'Half-life time of ozone as a function of air conditions and movement' 10TH INTERNATIONAL WORKING CONFERENCE ON STORED PRODUCT PROTECTION 2010,
OZKAN, R.; SMILANICK, J.L.; KARABULUT, O.A.: 'Toxicity of ozone gas to conidia of Penicillium digitatum, Penicillium. italicum, and Botrytis cinerea and control of graymold on table grapes' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 60, 2011, pages 47 - 51
MINAS, L.S.; KARAOGLANIDIS, G.S.; MANGANARIS, G.A.; VASILAKAKIS M.: 'Effect of ozone application during cold storage of kiwifruit on the development of stem-end rot caused by Botrytis cinerea' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 58, 2010, pages 203 - 210
PALOU, L.; CRISOSTO, C.H.; SMILANICK, J.L.; ADASKAVEG, J.E.; ZOFFOLI, J.P: 'Effects of continuous 0.3ppm ozone exposure on decay development and physiological responses of peaches and table grapes in cold storage' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 24, 2002, pages 39 - 48
TZORTZAKIS, N.; TAYBI, T.; ROBERTS, R.; SINGLETON, I.; BORLAND, A.; BARNES, J.: 'Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 61, 2011, pages 152 - 159
SELMA, V.M.; IBANEZ, M.A.; CANTWELL, M.; SUSLOWA, T.: 'Reduction by gaseous ozone of Salmonella and microbial flora associated with fresh-cut cantaloupe' FOOD MICROBIOLOGY vol. 25, 2008, pages 558 - 565
GRACIA-GARZA, J. A.; FRAVEL, D. R.: 'Effect of relative humidity on sporulation of Fusarium oxysporum in various formulations and effect of water on spore movement through soil' PHYTOPATHOLOGY vol. 88, 1998, pages 544 - 549
JOHNSON, E. M.; SUTTON, T. B.: 'Response of two fungi in the apple sooty blotch complex to temperature and relative humidity' PHYTOPATHOLOGY vol. 90, 2000, pages 362 - 367
SMILANICK, J. L.; MANSOUR, M. F.: 'Influence of temperature and humidity on survival of Penicillium digitatum and Geotrichumcitri-aurantii' PLANTDIS vol. 91, 2007, pages 990 - 996
CARROLL, J. E.; WILCOX, W. F.: 'Effects of humidity on the development of grape vine powdery mildew' PHYTOPATHOLOGY vol. 93, 2003, pages 1137 - 1144
ERKAN, M.; WANG, S.Y,.; WANG, C.Y.: 'Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 48, 2008, pages 163 - 171
OBANDE, M.A.; TUCKER, G.A.; SHAMA, G.: 'Effect of preharvest UV-C treatment of tomatoes (Solanum lycopersicon Mill.) on ripen ingand pathogen resistance' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 62, 2011, pages 188 - 192
GUAN, W.; FAN, X.; YAN, R.: 'Effects of UV-C treatment on inactivation of Escherichia coli 0157:H7, microbial loads, and quality of button mushrooms' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 64, 2012, pages 119 - 125
ROTEM, J.; WOODING, B.; AYLOR, D.E.: 'The role of solar radiation, especially ultraviolet, in the mortality of fungal spores' PHYTOPATHOLOGY vol. 75, 1985, pages 510 - 514
POMBO, M.A.; ROSLI, H.G.; MARTINEZ, G.A.; CIVELLO, P.M.: 'UV-C treatment affects the expression and activity of defense genes in strawberry fruit (Fragaria ananassa, Duch.' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 59, 2011, pages 94 - 102
CHENA, Z.; ZHUB, C.: 'Combined effects of aqueous chlorine dioxide and ultrasonic treatments on postharvest storage quality of plum fruit (Prunus salicina L.' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 61, 2011, pages 117 - 123
FLITSANOV, U.; MIZRACH, A.; LIBERZON, A.; AKERMAN, M.; ZAUBERMAN, G.: 'Measurement of avocado softening at various temperatures using ultrasound' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 20, 2000, pages 279 - 286
MIZRACH, A.: 'Nondestructive ultrasonic monitoring of tomato quality during shelf-life storage' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 46, 2007, pages 271 - 274
CAO, S.; HU, Z.; PANG, B.: 'Optimization of; postharvest ultrasonic treatment of strawberry fruit' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 55, 2010, pages 150 - 153
DAUS, A.; HOREV, B.; DVIR, 0.; SHALOM, S.; LICHTER, A.: 'The efficacy of ultrasonic fumigation for disinfestation of storage facilities against postharvest pathogens' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 62, 2011, pages 310 - 313
MIZRACH, A.: 'Ultrasonic technology for quality evaluation of fresh fruit and vegetables in pre- and postharvest processes' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 48, 2008, pages 315 - 330
GABLER, F.M.; SMILANICK, J.L.; MANSOUR, M.F.; KARACACA, H.: 'Influence of fumigation with high concentrations of ozone gas on postharvest gray mold and fungicide residues on table grapes' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 55, 2010, pages 85 - 90
KARACA, H.; WALSE, S.S.; SMILANICK, J.L.: 'Effect of continuous 0.3 pUL gaseous ozone exposure on fungicide residues on table grape berries' POSTHARVEST BIOLOGY AND TECHNOLOGY vol. 64, 2012, pages 154 - 159
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Attorney, Agent or Firm:
DESTEK PATENT, INC. (Konak Mah. Lefkose Cad. NM Ofis Park B, Blok No: 36 / 5 Besevler Nilufer, Bursa, 16110, TR)
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Claims:
CLAIMS

1. The invention is related to the system of application of humidity, ozone, various organic and inorganic chemicals and/or different physical applications together on fresh fruit and vegetables, and it is characterized in that it contains;

- humidity and humidifying unit (10) facilitating pathogens on fresh fruit and vegetables to become sensitive and to increase efficacy of ozone gas ,

- ozone gas and ozone gas connection unit (13) facilitating preservation of fresh fruit and vegetables for longer periods and much healthier way and to eliminate, sterilize and disinfection of pathogens ,

- treatment cabinet (17) facilitating to supply aforementioned application in combinations and to make measurements .

2. It is a treatment system complying with Claim , and it is characterized in that it contains; disinfectant inlet (9) facilitating supplying of disinfectants desired to be used inside the said treatment cabinet (17).

3. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the fan (24) facilitating the uniformed gas distribution inside said treatment cabinet (17) and the fan airflow conduction unit (25) facilitating transmission of the air sucked by said fan (24).

4. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the palette system (15) facilitating placing products desired to be treated inside the said treatment cabinet (17).

5. It is a treatment system complying with Claim 1 and 3, and it is characterized in that it contains; the collapsible cover (26) facilitating to make partitions in preferred volumes from the preferred part of said treatment cabinet (17).

6. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the air pump unit (21) facilitating to increase the air pressure inside said treatment cabinet (17) and the external air suction unit (22) facilitating supplying air from environment to be pumped by said air pump unit (21 ).

7. It is a treatment system complying with Claim , and it is characterized in that it contains; the water reservoir (1) to be used in humidifying inside the said treatment cabinet ( 7).

8. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the cooling mechanism (3) facilitating lowering temperature at will within the said treatment cabinet (17). 9. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the solid material inlet (16) facilitating to place antimicrobial compounds inside the said treatment cabinet (17).

10. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the connection unit for gas, liquid and disinfectants (7) enabling to supply gas, liquid and disinfectants into the said treatment cabinet (17) and the alternative gas connection unit (8) facilitating connection of alternative gas sources.

11. It is a treatment system complying with Claim 1 to 10, and it is characterized in that it contains; the pressure sensor (20) enabling to measure gas pressure in the said treatment cabinet (17) and the alternative gas sensor (6) enabling to measure alternative gas concentrations that may be supplied into the said cabinet. 2. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; the UV source (27) and the ultrasonic source (28) facilitating optional simulation with antimicrobial effect and IR (induced resistance) in the said treatment cabinet (17).

13. It is a treatment system complying with Claim 1 , and it is characterized in that it contains; an enclosed space, a modified atmosphere package (MAP) or a modified atmosphere palette cover (MAPo) where in the treatment cabinet ( 7) those products to be treated are to be placed in the said treatment cabinet (17).

14. It is a treatment system complying with Claim 1 to 13, and it is characterized in that it contains; said treatment cabinet (17) is portable and collapsible.

15. The invention is related to the application method of firstly humidity, and then ozone gas and those disinfectants or chemicals in various physical states, those compounds with antimicrobial effects, and different physical applications on to fresh fruit and vegetables and it is characterized in that it contains operation steps of;

- facilitation of supplying humidity at a certain levels to the system through the humidifying unit (10) ensuring increase of efficiency of ozone gas and the conversion of resistant pathogen spores on said fresh fruit and vegetables to sensitive vegetative cells,

- entrance of ozone gas into the system through the ozone gas connection unit (13) in order for facilitating to prevent pathologic and physiologic factors creating disease on said fresh fruit and vegetables, elimination of food-borne pathogens, decomposition of pesticide residues, microorganisms producing carcinogenic compounds and mycotoxins produced by those metabolites and fungi produced thereby,

- facilitation of aforementioned applications into the treatment cabinet (17) in combinations, and making measurements.

16. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of disinfectant entrance into the said treatment cabinet ( 7) through the disinfectant inlet (9). 17. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of placing those products to be treated into the said treatment cabinet (17) via the palette system (15).

18. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of supplying ambient air from environment through the external air suction unit (22) and increasing the air pressure in the treatment cabinet (17) through the air pump unit (21).

19. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of lowering the temperature in the treatment cabinet (17) through the cooling mechanism (3). 20. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of placing antimicrobial solid compounds into the treatment cabinet (17) through the solid material inlet (16).

21. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stages of supplying the mixture of gas, liquid and disinfectants into the treatment cabinet (17) through the connection unit for gas, liquid and disinfectants (7), and connecting alternative gas sources through the alternative gas connection unit (8). 22. It is a treatment system complying with Claim 15 and 21 , and it is characterized in that it contains the stages of measuring gas pressure in the treatment cabinet (17) through the by the pressure sensor (20), and alternative gas concentrations by the alternative gas sensor (6). 23. It is a treatment system complying with Claim 15 and 22, and it is characterized in that it contains the stage of discharging the gas in the treatment cabinet (17) through the vacuum system (18) after the treatment carried out for a given time and dose. 24. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of realization all aforementioned application with the collapsible cover (26), open and/or closed, in the treatment cabinet (17).

25. It is a treatment system complying with Claim 15, and it is characterized in that it contains the stage of facilitating antimicrobial effect into the said treatment cabinet (17) through the UV source (27) and the ultrasonic source (28).

Description:
DESCRIPTION

APPLICATION OF HUMIDITY, OZONE AND SEVERAL CHEMICALS TOGETHER

ON FRESH FRUIT AND VEGETABLES

Field of Invention

The invention is in regards to a treatment model enabling fresh fruit and vegetables harvested in fresh fruit and vegetables sector to be preserved in longer period of times with lesser pathological and physiological losses. The invention is related particularly with the treatment model enabling fresh vegetables and fruit harvested to be preserved long periods of time and with less pathological and physiological losses by means of applying different physical treatments together (application of Ultraviolet (UV) source- ultrasonic source- heat) as well as humidity, ozone and various chemicals.

Background of Invention

Presently, ozone gas is used for the purpose of performing sterilization of laboratory equipments, surgical tools and equipments, and such places as houses, work places, hospitals and hotel rooms, by combining at various humidity levels. In addition, ozone applications are used for elimination of microorganisms in agriculture and agricultural industry, which produce carcinogenic compounds increasing risk of carcinogenesis particularly in human beings. In background of the invention, there are various applications dealing with the issue. There are such disinfection and sterilization methods as ozone gas treatments, disinfection treatments in various physical conditions, ultraviolet methods, physical control methods, etc. However, due to that efficiency levels of application fields of such sterilization and disinfection methods are restricted; those technical problems encountered are not solved completely. Similarly, although there are treatments such as combining those aforementioned methods, due to both having them not reached to adequate efficacy and imposing both additional cost and labor as well as applicability issues, combined applications did not bring successes either. The most important disadvantages encountered in the background of the inventions are that capacity of sterilization and disinfection are either insufficient or ineffective. Nevertheless, having no preservation and control methods to overcome restricting factors such as particularly residue problems in sterilization and disinfection methods performed food products, paying attention to health of human, food and environment, using such methods suitable to practical and processing procedures is another important disadvantage. For overcoming aforementioned problems, it is necessary to have much more reliable and effective sterilization and disinfection methods or the combinations thereof. Another disadvantage of the existing technique is that since almost all of the existing applications are related to either sterilization or elimination of microorganisms producing carcinogenic matters, they cannot sufficiently prevent spoilage, deterioration and deformation in agricultural products caused by plant pathogens, and accordingly, preservation times for agricultural products are shortened. Such shortening of preservation times, in turn, causes commercial losses to take place.

In the background of the invention there are some applications for increasing efficacy of ozone gas which some treatments applied by combining with disinfectants. Nonetheless, no success was achieved either as anticipated from combinations of ozone gas - disinfectants. Due to having inadequate efficacy level and sterilization capacities, existing treatments have a disadvantage in terms of efficacy level. In the background of the invention, there are treatments where moisturizing procedure and supplying ozone gas are conducted simultaneously. However, supplying ozone gas simultaneously with humidity increases efficacy of ozone gas by very small ratios, and causes need of more ozone gas production. If it is needed to explain a little the disadvantage of the system, it is necessary to mention, first of all, such disadvantage encountered with supplying ozone gas and moisture simultaneously. In the present state, with the application of ozone gas simultaneously with humidification, efficacy of ozone gas will increase gradually in line with gradual increase of humidity level, and throughout the application period, it will not be possible to utilize efficacy level of ozone gas in full capacity. Likewise, in line with increase of ozone gas efficacy in time, longer application periods will be needed and there will be both economical and time losses because there will be need of more ozone gas production. One of the patents encountered with during patent searches in the literature with respect to the subject is the treatment entitled as "portable compact ozone generator" numbered TR2009/04998. In the summary of the invention, it is counted as related to portable compact ozone generator, and that the portable compact ozone generator being the subject matter of the invention is used for purposes of disinfecting water and beverage bottling, iron and manganese refining, food premises, cold storages, slaughterhouses, poultry breeding farms, poultry slaughter houses, fish processing facilities, fisheries, fruit & vegetable storage, denim discoloring, odor elimination, swimming pools, and such other places. Another treatment in relation with the subject is related to the invention entitled "development accomplished in ozone generator" and numbered TR2005/04202. In the summary of the said invention it is counted that the invention is related to the improvement made in the ozone generators used in disinfection of water, and for the purposes of disinfection in food industry, cold storages, swimming pools, medical and health services, waste water treatment, pharmaceutical factories, factories of chemical materials, fish processing facilities, rubber oil processing facilities, paper factories, textile sector, nitric ammonia elimination, iron & manganese elimination, pesticide elimination, odor elimination, discoloring, in short disinfection of every place of where we live and work. Another patent application related to the subject is the one entitled "procedure applied for treatment of vegetable substances with ozone" numbered TR2007/08002. In the summary of said application, it is mentioned of being related to a procedure containing at least the following stages for treating vegetables substances; preliminary moistening stage of vegetable substance by adding one volume of water, the conditioning stage for a vegetable substance moistened in this way, and the stage of contacting vegetable substance with ozone. During the search in the foreign databases in the literature, some patent applications conducted in relation to the subject were come across. In the first of these, as numbered CN101530124, it was mentioned of the method of preserving fruit and vegetables fresh using ozone. Beside the one above, in the application numbered as JP2009171889, also said treatments made with application of moistening of surfaces and ozone or alcohol facilitating sterilization and disinfection fruit and vegetables. Another patent application encountered with during patent searches in the literature with respect to the subject is the treatment entitled as "Method and Device for Ozone Sterilizing/Deodorizing" numbered JP2004173904A. In the summary of said patent application, it is expressed supplying ozone gas and humidity simultaneously. The application method of the invention is to supply ozone gas by bringing through a conduction channel and humidity from another conduction channel after combining them. The system states that efficacy of humidity and ozone gas is increased, but in the place of application, particularly humidity having values of 80% or more will increase both water consumption and the need of more production of ozone gas. However, it is expressed that where this is performed as ozone-humidity combination in much smaller particles, it is much effective and reliable method and that consumption of ozone gas and water will be reduced. In addition, there are some connection points in the system to enable vaporizing different liquid compounds and supplying them into an enclosed volume.

One of the basic disadvantages of the said system is that synergistic effect to take place interaction of ozone gas and humidity does not take place completely. In general, as result of interaction of ozone gas with water in the system, a sudden breakdown of ozone gas takes place and it loses its oxidative potential by the time it reaches to target area. On the other hand, direct supply of ozone gas not interacted with water particles does not make any difference from existing systems and bring any innovation to the same. That undissolved ozone gas displays insufficient efficacy as is the case in existing systems reduces the performance of the system. Moreover, misting ozone and humidity combination means the moving from gas phase to liquid phase. In such circumstances, while mist displays sterilization and disinfection effect where it comes to contact to target organisms or areas, it cannot do the same on those surfaces that it has not come to contact with, and accordingly, this causes reduction of efficacy due to lack of homogeneity. Another patent application encountered with during patent searches in the literature with respect to the subject is the treatment entitled as "Ozone Mist Generator and Refrigerator" numbered JP2007040681A. In the summary of this patent application, it is stated that ozone gas and water (mist) combination to be supplied together into vegetable compartment of refrigerators. To summarize the study of the patent- subject invention in general terms, the system transmits ozone gas obtained from an ozone production source and water supplied from a water chamber in the system into a compartment behind the vegetable compartment through a conduction line. After the ozone-water (mist) combination vaporized into the said chamber is performed through absorption via a conduction line, it is sprayed into vegetable compartment. Similarly, the system mixes ozone and water sources taken inside through 2 different lines in one conduction line, and facilitates the mixture to be sprayed directly into vegetable compartment through the said single line. Some of ozone gas that is supplied into water line is diffused in water. Part of ozone gas that is not diffused in water is broken down by ozone breakdown system and is discarded out of the system. Similarly, hydroxyl radicals are formed by the rapid breakdown of ozone gas inside the ozone-water line. The structure that is turned into ozone-water in the water line is delivered into vegetable compartment from ultrasonic disintegrators. On the other hand, as a different production technique, water is first of all vaporized into a different chamber through an ultrasonic disintegrator. Also by injecting ozone gas into the chamber where water misting is performed, more effective and lesser concentrated mist is obtained by speeding up in the water molecules the dissolution of ozone gas which has smaller particle structure. In this system, primarily procedures of ozone gas production and water production or misting of water take place simultaneously. In our invention for the patent application subject, primarily supplying ozone gas into the designated space is performed after the saturation of target space with humidity. Although there is no simultaneous supply of ozone gas and humidity, as mentioned, between the patent application numbered JP2007040681A and our application, it is aimed to have oxidative characteristic of ozone gas displayed directly on target organisms or compounds. In addition, misting after dissolution of ozone gas in water or misting after combining in a given environment are both completely different than our application model. In the other systems mentioned, ozone gas half-life is rapidly reduced by interaction of ozone gas with water, and the efficacy thereof gradually decreases. With the shortening of half- life, those compounds required to display efficacy (hydrogen peroxide, hydroxyl radicals, etc.) lose their oxidative characters partially or completely before reaching to target organisms or spaces. Moreover, in the aforementioned systems, there occurs very low concentration levels of ozone gas production and such concentrations are insufficient with respect to capacities to break down target organisms or compounds. Another patent application encountered with during patent searches in the literature with respect to the subject is the treatment entitled as "Utility model modified atmosphere vacuum conditioning cabinet" numbered CN201028914Y. In the patent summary, it is stated of performing sterilization with supplying ozone gas in a cabinet facilitating preservation of produces. In the implementation method of said patent application, it is recounted of moisturizing procedure for preventing water loss and maintaining freshness of such products, as particularly vegetables and fruit, that loss water during preservation. Of the three different storage shelves, on the topmost shelf vegetables and fruit, on the middle shelf meat and meat products, and on the lowest shelf such products as grain are stored. For sterilization on those products stored inside the cabinet, each cabin is given ozone gas and sterilization is performed using ozone gas. In the patent application numbered CN201028914Y, ozone is applied inside an enclosed volume. Although no sufficient efficacy is obtained from ozone gas with only ozone treatment or without using treatments increasing efficacy of ozone gas, it is necessary to apply treatments at very much higher ozone gas concentrations for obtaining better efficacy in comparison to those treatments of ozonization carried out under moistened conditions. Due to the said requirement, more energy is consumed as well as the costs thereof increased depending on more ozone gas production. Similarly, not enough oxidative potential and antimicrobial effect may be reached as well as better efficacy not being obtained in comparison to supplying ozone gas in moistened conditions when and if high concentrations are used. In the aforementioned patent applications, half-life of ozone is shortened by moistening or providing water simultaneously with supplying ozone gas, and its oxidative potential is decreased or completely diminished. Moreover, simultaneous supply procedure performed in general for increasing the amounts of hydrogen peroxide or hydroxyl radicals is valid neither in theory nor in practice. Breakdown of ozone gas becomes more of an issue in terms of both human health and environmental safety. In line with -this purpose, ozone gas is generally supplied together with water. However, in the patent applications summarized in background of the invention, sterilization capacity of ozone gas depending on its oxidative character is reduced and its breakdown is ensured by performing ozonization and moistening procedures at the same time. Existing inventions means that such simultaneous supply is applied for forming of hydroxyl radicals. However, although oxidative potentials of hydroxyl radicals are higher than ozone gas, half-lives of the former are much shorter comparing to that of ozone gas. Although half-life of ozone gas varies depending on the environmental conditions and despite it is generally stated in minutes, half-lifes of hydroxyl radicals are in much shorter times, such as 10 "9 seconds. As result of this, where utilization of sterilization or oxidative characters of hydroxyl radicals is thought, it is necessary to ensure that hydroxyl radicals occur in contact with target organisms or areas. The summarized patent applications in background of the invention do not overcome the problem mentioned in relation to half-life and efficacy level of hydroxyl radicals in general, and sterilization and oxidation eliminate or reduce the utilization chance from high efficacy of these radicals. Objective of the Invention

The present invention is related to a treatment model facilitating preservation of fresh vegetables and fruit harvested for longer period of time and with lesser loss by means of applying humidity, ozone and various chemicals or different physical treatments all together on fresh vegetables and fruit, a treatment that meets the aforementioned requirements, eliminating all disadvantages and bringing some additional advantages.

One goal of the invention is to eliminate by ozone gas those microorganisms causing reduction in preservation period for fresh vegetables and fruit, and to ensure extension of preservation period for produces by breaking down ethylene gas, which causes aging of produces, using ozone by means of the subject treatment of the invention. Another goal of the invention is to ensure prevention of spoilage of fresh vegetables and fruit, originating from microbial activities by means of the subject treatment of the invention. Another goal of the invention is to ensure elimination of animal and vegetable food- borne pathogenic microorganisms causing immune system problems and various diseases in human beings and animals. Another goal of the invention is to prevent spoilage originating from microbial activities by applying firstly humidity and then ozone gas as well as disinfectants or chemicals, compounds with antimicrobial effect in various physical forms, different physical treatments, plant growth regulators or inhibitors, ethylene absorbent, compounds with IR (Induced Resistance) together under controlled or modified atmosphere conditions.

Another goal of the invention is to ensure breakdown of pesticide and chemical residues remaining on food products and fresh vegetables and fruit by means of the compounds with high oxidative potentials.

Another aim of the invention is to prevent ethylene production, which increases aging and susceptibility of harvested fresh vegetables and fruit to pathogens and is produced by the latter during preservation, and to break down ethylene in the environment by making use of oxidative character of ozone gas and other compounds formed.

A goal of the invention is to reduce percentage of spoilage originating from aforementioned plant diseases by means of the subject treatment of the invention. Another goal of the invention is to develop a treatment cabinet for realization of said treatment, in which said treatment is to be performed.

Another goal of the invention is to ensure disinfection of such produces and sterilizing the same by the subject treatment of the invention.

The subject invention and the treatment method of the patent application is designed to ensure for obtaining the optimal oxidative and sterilization efficacy. The subject treatment of the invention is a treatment beyond the background of the invention or solving the technical defects and failures of the background of the invention, and the most important difference of which is to enable sterilization and oxidation capacity thereof having remained at the optimal level under all the conditions. Similarly, it uses three different sterilization and oxidation mechanism at the same time by utilizing oxidative characters of all, ozone gas, hydrogen peroxide and hydroxyl radicals. Similarly, it reduces half-life of ozone gas and minimizes adverse effects of ozone gas as well as utilization of oxidative features of ozone gas. By combining humidity and ozone gas on the target it is ensured to utilize of efficacy of those compounds (hydrogen peroxide, hydroxyl radicals, etc.) with high oxidative potentials at the maximum levels. Besides, by the interaction of humidity and ozone gas within the target area and after increase of relative humidity levels, gradual increase in the efficacy of ozone gas under increased humidity conditions is prevented so that it becomes possible to utilize the efficacy of ozone gas in the shortest time and at the highest levels possible. It is known that efficacy of ozone gas increases with humidity. The reason for this is that ozone gas converts into such compounds with high oxidative potentials. However, if the procedure of increasing the humidity or moistening in the environment is applied simultaneously with ozone gas, efficacy of ozone gas also increases gradually in line with increasing humidity levels. As result of such applications, basically time is wasted. The main reason for losing time is that ozone gas may only reach to such concentrations for displaying its efficacy only after humidity reaches to certain levels, and in the duration until humidity level increases, ozone gas is not utilized sufficiently. Similarly, efficacy of ozone gas will not be utilized at high levels during the time humidity concentrations reach to desired levels. The primary reason for this is that when supply of ozone gas is provided simultaneously with humidity, half-life of ozone gas is rather shortened and concentration of ozone gas broken down is reduced in the unit volume. Accordingly, in order to increase ozone gas concentration in the unit volume or to have it go up to such desired concentrations, it is necessary to supply ozone gas from the ozone gas generator for longer periods and at higher amounts. In order to facilitate increasing the concentration of ozone gas in the unit volume, it is necessary to produce more ozone gas. In said process, much more energy consumption takes places and accordingly cost of use increases as result of utilizing additional time as well as depleting necessary sources for ozone gas production. In the application of the subject invention, its is ensured of increasing susceptibilities of target microorganisms as well as increasing efficacy of ozone gas by supplying humidity firstly. When these microorganisms are dormant they are more resistant against ozone gas or oxidative compounds. However, by supplying humidity into the environment where microorganisms are present such microorganisms are simulated and turn to be much more sensitive. Consequently, ozone gas reaches to much higher sterilization capacity. On the other hand, supplying humidity into the area of treatment or utilization before ozone gas and increasing relative humidity are used in all the fields where produce preservation is made commercially and are performed for reducing water loss during the preservation period of produces. Also in the said application of the subject invention, supplying humidity firstly complies with the conditions of commercial usage, it's not creates additional cost factor when used. In conclusion, having provided facilitating the utilization of ozone gas efficacy at optimum levels; ensuring to make use of efficacy of such compounds with oxidative potentials at the most appropriate place and time, and accordingly, increasing sterilization capacity; reducing the costs; it eliminates those technical and practical disadvantages of all the patent applications that were come across during the literature search conducted in relation to the subject. Structural and characteristic features and all the advantages of the invention will be much more clearly understood with the figures given below and the detailed explanations written by referencing to those figures and it is therefore necessary to do the evaluation by taking into account those figures and the detailed explanations.

Brief Description of the Drawings

In order for understanding the constructing of the present invention and the advantages thereof together with its additional elements, it is necessary to evaluate it together with the figures that are explained below.

Figure 1 : A schematic display of the subject treatment of the invention on fresh vegetables and fruit, by means of firstly humidity and then ozone gas as well as disinfectants or chemicals, compounds with antimicrobial effect in various physical forms, different physical treatments, plant growth regulators or inhibitors, ethylene absorbent, compounds with IR (Induced Resistance) under controlled or modified atmosphere conditions. Figure 2: Schematic presentation of the application of the subject invention on fresh vegetables and fruit with humidity, ozone or various chemicals within a modified atmosphere package (MAP) or a modified atmosphere pallet cover (MAPo).

Drawings are not necessarily to be scaled and in order for understanding the present invention, unnecessary details may have been omitted. In addition, such elements at least substantially identical or at least having substantially identical functions are marked with the same number.

Reference Numbers

1. Water reservoir

2. Temperature sensor

3. Cooling mechanism

4. Water connection unit

5. Fan airflow inlet unit

6. Alternative gas sensor

7. Connection unit for gas, liquid and disinfectant

8. Alternative gas connection unit

9. Disinfectant inlet

10. Humidifying unit

11. Humidity sensor

12. Ozone sensor

13. Ozone gas connection unit

14. Ozone gas decomposition unit

15. Palette system

16. Solid matter inlet

17. Treatment cabinet

18. Vacuuming system

19. Vacuuming system control valve

20. Pressure sensor 21. Air pump unit

22. External air suction unit

23. Fan suction unit

24. Fan

25. Fan airflow conduction unit

26. Collapsible cover

27. UV Source

28. Ultrasonic Source

29. Door

30. Automatic locking system

31. Automatic locking system - red warning lamp indicating the locking system is active.

32. Automatic locking system - green warning lamp indicating the locking system is inactive.

33. Gas outlet unit

Detailed Explanation of Invention

In this detailed explanation, preferred structures in the system of applying humidity, ozone or various chemicals or different physical treatments together on fresh vegetables and fruit as in the invention subject are explained only for better understanding of the subject as not to create any limiting effect.

If the application of the invention is approached on the basis of elements, firstly, it has a treatment cabinet (17) in which all the treatments are to be performed in combination and the measurements are to be made. The said treatment cabinet (17) is in form of an enclosed volume in the preferred applications of the invention and all the treatment process takes place therein. In various applications for the invention, the said treatment cabinet (17) may be made of different materials and of different forms. In the invention, water reservoir (1 ) is the water source to be used in humidifying process. For lowering the temperature in the treatment cabinet (17), there is a cooling mechanism (3 therein. The temperature inside the treatment cabinet (17) is measured via a temperature sensor (2). In the application of the subject invention, connections of alternative gas-sources to inside of the treatment cabinet (17) are performed by an alternative gas connection unite (8). Measuring the concentrations of such alternative gases that may be supplied inside the treatment cabinet (17) is performed by an alternative gas sensor (6). In addition, there is a connection unit (7) for supplying compounds of gas, liquid and disinfectant inside the said treatment cabinet (17).

In the application of the subject invention, there is a disinfectant inlet (9), to which disinfectants to be used in the treatment cabinet are to be connected. In addition, there is a humidifying unit (10) providing humidity to be supplied into the treatment cabinet (17) at any desired level. Level of humidity inside the treatment cabinet (17) is measured by a humidity sensor ( 1 ). Beside the said humidity sensor (1 1), there is an ozone sensor (12) that measures ozone concentrations in the treatment cabinet (17). There is an ozone gas connection unit (13) facilitating inside the treatment cabinet (17) the supply of ozone gas, the concentration of which is measured by the said ozone sensor (12). There is an ozone gas decomposition unit (14) facilitating decomposing down ozone gas inside the treatment cabinet (17) while it goes out of the cabinet (17) after the treatment. Another important part developed by the invention is pallet system (15). Such pallet system (15) is the part facilitating to place those produces to be treated on pallets inside the treatment cabinet (17). In the treatment cabinet (17), there is a solid matter inlet (16) facilitating to place solid compounds to be utilized for antimicrobial efficacy thereof. In the said treatment cabinet (17), there is a vacuuming system (18) used for lowering any increased gas pressure. Such vacuuming system (18) is controlled by a vacuuming system control valve (19). The gas pressure inside the said treatment cabinet (17) is measured by a pressure sensor (20). There is an improved air pump unit (21 ) for pumping air toward inside to increase air pressure that is lowered in the treatment cabinet (17). For pumping the air toward inside, there is an external air suction unit (22) enabling to get air from external environment. Beside these, there is a fan (24) distributing gas homogeneously in the treatment cabinet (17). The inlet of this fan (24) inside the treatment cabinet (17) which is to be used for distributing gases homogeneously inside the treatment cabinet (17) is fan air flow inlet unit (5). The fan (24) providing such homogeneous gas distribution has a fan suction unit (23) sucking air from inside the treatment cabinet (17). In addition, this fan system (24) has a fan air flow conduction unit (25) that lets the air sucked from inside the cabinet (17) to the treatment cabinet (17) again through connection pipes. There are gases accumulated in the treatment cabinet (17) after the treatment. Ozone gas among such gases is broken down by ozone gas decomposition unit (14) and is thrown out through the vacuuming system together with all other gases.

Application Method of the Invention; In the invention, fresh vegetables and fruit into the treatment cabinet (17) are placed on pallets, in bulk or in crates. Afterwards, humidity supply from humidifying unit (10) and ozone gas from ozone gas connection unit (13) is transferred into the system when the door (29) of the treatment cabinet (17) is closed. During this procedure, supplying humidity in the first place is very important for the treatment. In the treatment cabinet (17) mentioned in the preferred applications of the invention, firstly humidity is brought to a certain relative ratio, and then ozone gas is transferred into the system.

It is a scientific reality that the efficacy of ozone gas increases with humidity.

Diagram- 1a: Reaction diagram showing the compounds originating as interaction of ozone gas with humidity.

0 3 + H 2 0 HO + OH H 2 0 + 0 2

(ozone)

Diagram- 1 b: Reaction diagram showing the compounds originating as result of interaction of ozone gas with hydrocarbon compounds. OH "

(hydrocarbon)

After ozone gas interacts with humidity, ozone gas causes the formation of hydrogen peroxide (H2O2) and hydroxyl radicals (OH " ) that have strong oxidative and antimicrobial effect. This chemical conversion is repeatable and continuous conversion, and after interaction of ozone gas with humidity, antimicrobial and oxidative characteristics of both ozone gas (O3) and hydrogen peroxide (H 2 0 2 ) as well as of hydroxyl radicals (OH " ) and ions are utilized in environment. In conclusion, efficacy is increased (J. Chen and P. Wang, 2005). Likewise, there are scientific literature indicating that fungi toxic activity of ozone gas is increased at relative humidity concentrations of 70% and more (Ozkan at al., 2011). It is known that bactericide and virucide activity of ozone gas is high. At the same time, hydroxyl radicals and hydrogen peroxide also have very strong antimicrobial and high oxidative properties. These compounds are formed as result of interaction of ozone gas with humidity. With the subject treatment model, oxidative and antimicrobial properties of not only ozone gas but also hydrogen peroxide and hydroxyl radicals are utilized and the efficacy thereof increases only depending on ozone gas utilization. The reason for increase in sterilization capacity of ozone gas is that ozone gas converts much more to such compounds as hydrogen peroxide and hydroxyl radicals displaying stronger oxidative potentials during interaction with humidity, and increase in its conversion rate. Thus, it is possible to utilize three compounds with more oxidative potential at the same time, and sterilization capacity increases. In the subject treatment method, another main reason for using ozone gas together with particularly humidity is to utilize efficacy of aforementioned compounds. Moreover, as result of scientific researches, it has been found that oxidative effect of ozone gas doses at increasing relative humidity levels during interaction of ozone gas and humidity is more than the oxidative effect of the same ozone doses at lower relative humidity levels. In the light of the information provided in the background of the invention, supplying ozone gas and humidity simultaneously restricts efficacy of ozone gas. The reason for this is that relative humidity will increase gradually in an environment to where mixture of ozone and humidity is supplied, and during such gradual increase, ozone gas activity will also gradually increase. Supplying ozone gas together with humidity will shorten half-lifes of ozone gas and hydroxyl radicals and consequently, the efficacy thereof will be reduced. For ozone gas and humidity are supplied together, when ozone gas meets humidity as soon as coming out of ozone source, ozone gas will react with humidity very quickly and the half-life will start in a very short time. During the time elapsed from the environment where ozone gas and humidity interact to where the application will take place, ozone gas and hydroxyl radicals will continuously half-life. In conclusion, where ozone gas and humidity are supplied simultaneously, it is not possible to be fully benefited from the efficacy of ozone gas. Therefore, to increase relative humidity of the environment prior to supplying ozone gas increases efficacy of ozone gas more. Besides, another reason for supplying ozone gas into treatment cabinet (17) only after humidity reaches to certain level is that particularly resistant pathogen spores will become much sensitive with the effect of increasing humidity (J. A. Gracia-Garza and D. R. Fravel 1998, Johnson, E. M. and Sutton, T. B. 2000, Smilanick, J. L. and Mansour, M. F. 2007). Moreover, particularly for storage of fresh vegetables and fruit after harvest, having lost water physiologically increases both pathogen sensitivity of products and shortens the storage times of products. That the products interact firstly with humidity will lengthen the storage times thereof. Humidifying on the places where produces are stored or directly onto produces is performed for purposes of preventing water loss caused by respiration and evaporation of produces. Similarly, during preservation of produces, storage conditions generally humidify under commercials conditions. Thus, there will be no difficulty or any additional cost in transferring the invention to the industry with respect to particularly performing j!moistening. Therefore, in the preferred application of the invention, supplying ozone gas into the system only after the environment is humidified sufficiently at approximately 60-100%. In the preferred application of the invention relative humidity is 90-100%. Increase in humidity and proper humidity conditions are monitored by humidity sensor (11). As stated in the subject of the invention, combinations of the following are applied; together with said humidity and ozone gas, disinfectants or chemicals, compounds with antimicrobial effect in various physical forms, different physical treatments, plant growth regulators or inhibitors, ethylene absorbents, compounds with IR (Induced Resistance) under controlled or modified atmosphere conditions. Such disinfectants are supplied into the treatment cabinet (17) through disinfectant inlet (9). In addition, combinations of gas, liquid and disinfectant are given inside the treatment cabinet (17) through a connection unit (7) for such combinations of gas, liquid and disinfectant.

In the experimental studies in the Table 1 below, fungal decay rates as given % fruit decay in comparison to control fruit as result of different treatments. In the table are given spoilage amounts and percentages in fruit infected with the plant pathogen agent, Botrytis cinerea (gray mold) causing spoilage in almost all vegetables and fruit that have very high saprophytic property and are polyphagous (having multi-host).

Those fruit used as control group are the ones not treated with ozone gas alone, humidity-ozone gas or disinfectant. In the experimental study conducted, all fruit were inoculated with the pathogen, Botrytis cinerea and the effects of ozone gas alone, humidity-ozone gas, disinfectant and the combinations thereof on gray mold are given as % fruit decay. As result of the experiment carried out, efficacy of humidity- ozone gas and disinfectant was tried one by one and as combinations of each other at different durations. In conclusion, gray mold-based % fruit decay in all treatments was reduced in comparison to control fruit. The most effective results however were obtained from humidity-ozone gas and disinfectant combination. In the trial, it is observed that humidity-ozone gas treatments reduced percentage of fruit decay much more effectively comparing to the treatment of ozone gas alone. With the treatment of humidity-ozone gas and disinfectant (PAA, peracetic acid) together in combination, there was much more efficacy of humidity-ozone gas or disinfectant than displayed by them separately, and a synergistic effect was observed among them in reducing fruit decays. In conclusion, it was observed that besides having much more effective comparing to those treatments of ozone gas alone, humidity- ozone gas treatments obtained much more successful results, and the efficacy increased more with the addition of disinfectant to humidity-ozone treatments.

Table 1 : Number and percentage of decay fruit in 2 different kinds of pear, (Santa Maria and Deveci), treated with ozone gas together with humidity and disinfectants for different periods and inoculated with Botrytis cinerea (gray mold).

* PAA, Peracetic acid.

"minutes, treatment time in minutes

In the trial, all the fruit was inoculated through wounded made at 3 mm in depth and 1 mm in width from all around of each fruit after disinfection procedure with gray mold at concentrations of 1x10 5 spores/ml.

Fruit was taken into treatment after inoculation procedure, excluding controls.

After treatment, fruit was stored at 20°C for 3 days and then, decayed fruit was counted.

Relative humidity used in ozone treatments is 95%; only ozone treatment is conducted at 45% humidity level. Water requirement needed for humidifying procedure in the application of the subject invention is provided by water reservoir (1). In the treatment, fan (24) has an important role. Said fan (24) facilitates homogeneous gas distribution inside the treatment cabinet (17). The gas is sucked by the fan suction unit (23) facilitating such homogeneous distribution by suction in the cabinet and the sucked gas is again conducted to inside the treatment cabinet (17) by the fan air flow conduction unit (25). The conducted gas therein enters into the treatment cabinet (17) by means of the fan air flow inlet unit (5). Besides this, increased gas pressure inside the treatment cabinet (17) is lowered by the vacuuming system (18). Such vacuuming system (18) is controlled by the vacuuming system control valve (19) . The gas pressure inside the said treatment cabinet (17) is measured by a pressure sensor (20). When air pressure in the treatment cabinet (17) is low (due to low pressure occurrence in the treatment cabinet by extracting gases accumulated inside the treatment cabinet after the treatment process), pumping air into the treatment cabinet (17) is performed by air pumping unit (21). Such air needed for this purpose is supplied by the external air suction unit (22).

In the application of the subject invention, for lowering the temperature in the treatment cabinet (17) when needed, a cooling mechanism (3) is used. The temperature inside the treatment cabinet is measured via a temperature sensor (2).

In the preferred application of the invention, it is enabled to place solid matters to be benefited from antimicrobial properties through the solid matter inlet (16) in the treatment cabinet ( 7).

In the preferred application of the invention, UV treatment is performed inside the treatment cabinet (17) by means of the UV source (27) facilitating simulation with antimicrobial effect and IR (Induced Resistance). Such UV source (27) facilitates radiations of 520 nm and lower wavelengths. With the radiations of such wavelengths (185 nm and lower wavelengths) from UV source (27), water (H 2 0) breakdoen into its (H + , OH " ) and yields to formation of such compounds with high oxidative potential. In addition, such UV source (27) breaks down ozone gas (0 3 ) at such wavelengths (185-300 nm), and converts it to oxygen (0 2 ) and oxygen atom (O " ). Oxygen atom emerging by breakdown of ozone gas reacts with water and provides formation of such compounds with high oxidative potentials (hydroxyl radicals, etc.). In addition, with the radiation from the UV source (27) at such wavelengths, antimicrobial effect (253,7 nm) is facilitated and thus, facilitates resistance to produces against pathogens by providing IR (induced resistance) in produces. Another advantage of UV source (27) is to maintain freshness of preserved produces by providing continuity of photosynthesis (400-520 nm). In the preferred applications of the invention, ultrasonic treatment is performed by means of the ultrasonic source (28) facilitating simulation with antimicrobial effect and IR (Induced Resistance) after produces are placed inside the treatment cabinet (17).

During the preferred application of the invention, automatic locking system (30) is activated after the door (29), which is used for placing and removing produces in and out of the treatment cabinet (17), is closed upon placing produces to be treated inside. All during the application period, the door (29) of the treatment cabinet (17) is locked by the automatic lock system (30) and is not opened until the treatment is completed. All during this process, a red warning light (31) on the door is on indicating that the lock is active and the treatment is in process. After completion of the treatment, the red warning light (31) of active lock-system continues to be on until all ozone gas in the treatment cabinet (17) is broken down by the ozone gas decomposition unit (14). After all the ozone gas inside the treatment cabinet (17) is broken down by the ozone-gas decomposition unit (14), the automatic lock system (30) is deactivated and the green warning light (32) of the automatic lock system becomes on and the door is unlocked.

There is an alternative gas connection unit (8) to which alternative gas sources to be connected in the invention. Concentration of such alternative gases is measured by means of the alternative gas sensor (6). In the application of the subject invention, the treatment cabinet (17) may be used as whole or as divided. Divided use of the application in the subject invention is performed by means of collapsible cover (26). This collapsible cover (26) may be places to any place to form any volume inside the treatment cabinet (17). Such divided application facilitates enabling the treatment of ozone gas and humidity combination to be supplied in form of shocking (certain dosage to be given suddenly). The basic principle here is to make use of the inverse proportion of (volume x dosage). After any desired humidity level is reached when the collapsible cover (26) is closed, the treatment cabinet is filled with high concentrations of ozone gas. Afterwards, the collapsible cover (26) is opened and ozone concentration is distributed into the treatment cabinet (17) homogeneously. This divided application provides to use such application dosages that would not be possible to reach or would take time to reach if a whole volume application is performed. The time needed for while reaching to high concentrations by closing the collapsible cover (26) is not necessary and any desired amount of ozone is directly supplied to the system. This way, the chance of phytotoxicity (damages occurring on produces as result of external treatments) on produces decreases.

Into the treatment cabinet (17), when the collapsible cover (26) is open, firstly humidity and then ozone gas, disinfectants or chemicals, compounds with antimicrobial effect in various physical forms, different physical treatments, plant growth regulators or inhibitors, ethylene absorbent, compounds with IR (Induced Resistance) are in direct contact with produces under controlled or modified atmosphere conditions. As result of this, produces during the preliminary setup time (time needed for ozone concentration to reach to any desired level) interact without losing time with humidity and ozone gas mixture and all other additional compounds and treatments. The total application time to be spent for this purpose is less than what is needed for the divided system. In the preferred application of the invention, the treatment cabinet (17) may be in form of a modified atmosphere pallet cover (MAPo) and a modified atmosphere package (MAP) or in any form to provide formation of an enclosed volume. In this application, under these pallets (15) the bottom part of MAPo is placed and on top of it, pallets or crates in which there are fruit or vegetables are placed. Afterwards, a MAPo in size to cover crates or pallets is covered from top to bottom. Following this, gaps are left for the ozone gas connection unit (13) and the humidifying unit (10) at the joints of two MAPo covers. Except these gaps, top and bottom parts of MAPo are sealed with sealing tapes. From both side of that MAP cover, holes are opened for the fan (24) to facilitate homogeneous gas distribution. Firstly humidity is given into the treatment cabinet (17) and then, when humidity reaches to the desired level, ozone gas is given. Optionally, efficacy of the basic application process may be increased by connecting alternative gases with antimicrobial effect to the system through the alternative gas connection unit (8) and/or through the disinfectant inlet (9) for disinfectants. From the beginning to the end of this process, the fan system (24) runs actively. At any desired dosage and times, entries from the treated pallet (15) are extended, and the holes prepared are sealed. Later on, the top and bottom of MAPo's of the pallets (15) treated and for which the application is completed are sealed to each other with tapes and the pallets or crates with MAPo holding produces are sealed with sealing tapes at the points of ozone and humidity entries, and the treatment process is completed. With the preferred application of the invention, reaction of ozone gas with humidity is utilized for longer period of time. MAP application enables to utilize oxidative property of ozone gas first of all until ozone gas reaches to a given concentration with the supply of ozone gas and humidity combination, and then during the application duration at desired dosage, and finally, even after completion of applications at desired levels eventually. By the virtue of this application, oxidative property of ozone gas stays in packaged and enclosed MAP or MAPo and continues to display its effect. When it is necessary to release gas, it is released through the gas outlet unit (33).

In the preferred applications of the invention, the treatment cabinet (17) may be mobile or portable. The application dimensions of the system may be adjustable depending on the usage capacity and allows operating in all desired volumes. The application cabinet (17) may be in cold storage places, in the cooling cabinets wherein heat insulation is maintained or on trucks, trailers and containers. It is possible to use them in slaughterhouses, processing plants for milk and dairy products, food processing and packaging plants. Similarly, it is possible to use them in all structures having a cooling unit and a certain enclosed volume used for preservation of produces. It is also possible to apply the same working principle by making mobile and small-scale models of such treatment cabinets (17).

In the preferred application of the invention, disinfectants applied in such treatment cabinet (17) are peracetic acid (PAA), hydrogen peroxide (H2O 2 ), sodium hypochlorite (NaOCI) and other hypo chlorites, chlorine dioxide (CIO 2 ), citric acid (C6H 8 0 7 ), acetic acid (CH3COOH), carboxylic acid, alcohols, alcohol derivatives, methyl alcohol (methanol), ethyl alcohol (EtOH), organic acids whether or not vegetable or animal origin and chemical disinfectants. In the preferred applications of the invention, alternative gases applied in such treatment cabinet (17) are sulfur dioxide (S0 2 ), carbon dioxide (C0 2 ), oxygen (O2), nitrogen dioxide (N0 2 ) and nitrogen, iodine gas and iodic compounds and biofumigants. Those solid matters with antimicrobial effect to be used in the preferred applications of the invention are Sodium bicarbonate (NaHC0 3 ) and similar salts, Sodium carbonate (Na 2 C0 3 ), Potassium monophosphate (KH 2 P0 4 ), Potassium diphosphate (K 2 HP0 4 ), Sodium silicate and similar salts, Potassium iodide and those compounds causing formation of iodine and halogen derivatives as a reaction result.

The concentration of ozone gas to be supplied into such treatment cabinet (17) may vary between 0.01 and 50.000 ppm. In addition, although duration of the treatment may change depending basically on physiology, sensitivity to form phytotoxic reaction and ripeness of produces to be treated, it has a very wide range of time interval varying between 5 seconds and 1 year (Palou et al., 2001 ; Palou et al., 2002; Tzortzakis et al., 2006; Minas et al., 2010; McClurkin, Maier et al., 2010; Minas et al., 2010).

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