Title:
APPLICATION OF MESENCHYMAL STEM CELL OSTEOGENIC DIFFERENTIATION INDUCTION SOLUTION AND PIPERAZINE
Document Type and Number:
WIPO Patent Application WO/2024/016492
Kind Code:
A1
Abstract:
Provided is a mesenchymal stem cell osteogenic differentiation induction solution, comprising a culture medium and piperazine. On the basis of a traditional induction solution, piperazine is used to replace dexamethasone, achieving the effects of osteogenic differentiation and osteogenic mineralization of mesenchymal stem cells. Piperazine is not considered to be a corticoid, has a relatively small influence on other cell metabolism, and industrial preparation of piperazine is simple and inexpensive. In addition, on the basis of piperazine having the effect of promoting osteogenic differentiation and mineralization, piperazine can be developed as a potential medication for treating bone defects, osteoporosis and other bone-related diseases; moreover, piperazine can be used as a small-molecule substance and loaded onto a carrier to be used for achieving the functions of osteogenic differentiation and mineralization.
Inventors:
RUAN CHANGSHUN (CN)
ZHANG QIAN (CN)
YANG JIRONG (CN)
LIU JUAN (CN)
HU NAN (CN)
ZHANG QIAN (CN)
YANG JIRONG (CN)
LIU JUAN (CN)
HU NAN (CN)
Application Number:
PCT/CN2022/125294
Publication Date:
January 25, 2024
Filing Date:
October 14, 2022
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
SHENZHEN PEOPLES HOSPITAL (CN)
SHENZHEN PEOPLES HOSPITAL (CN)
International Classes:
C12N5/077; A61F2/28; A61K31/495
Foreign References:
| CN104629046A | 2015-05-20 |
Other References:
MA YUFEI, HU NAN, LIU JUAN, ZHAI XINYUN, WU MINGMING, HU CHENGSHEN, LI LONG, LAI YUXIAO, PAN HAOBO, LU WILLIAM WEIJIA, ZHANG XINZH: "Three-Dimensional Printing of Biodegradable Piperazine-Based Polyurethane-Urea Scaffolds with Enhanced Osteogenesis for Bone Regeneration", APPLIED MATERIALS & INTERFACES, AMERICAN CHEMICAL SOCIETY, US, vol. 11, no. 9, 6 March 2019 (2019-03-06), US , pages 9415 - 9424, XP093131127, ISSN: 1944-8244, DOI: 10.1021/acsami.8b20323
RUAN CHANGSHUN, HU NAN, MA YUFEI, LI YUXIAO, LIU JUAN, ZHANG XINZHOU, PAN HAOBO: "The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 7, no. 1, US , XP093131129, ISSN: 2045-2322, DOI: 10.1038/s41598-017-06354-1
LEI, ZHIMIN ET AL.: "Chondrogenic Differentiation and Osteogenesis Gene Expression Following a Short-Time Induction of Adipose-Derived Stem Cells with Bone Morphogenetic Proteins", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH JUNE, vol. 13, no. 32, 6 August 2009 (2009-08-06), pages 6276 - 6278, XP009552076, ISSN: 2095-4344
PANJLA APURVA, QAYOOM IRFAN, KUMAR ASHOK, VERMA SANDEEP: "Anionic diketopiperazine induces osteogenic differentiation and supports osteogenesis in a 3D cryogel microenvironment", CHEMICAL COMMUNICATIONS, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 57, no. 60, 27 July 2021 (2021-07-27), UK , pages 7422 - 7425, XP093131137, ISSN: 1359-7345, DOI: 10.1039/D1CC01985F
RUAN CHANGSHUN, HU NAN, MA YUFEI, LI YUXIAO, LIU JUAN, ZHANG XINZHOU, PAN HAOBO: "The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 7, no. 1, US , XP093131129, ISSN: 2045-2322, DOI: 10.1038/s41598-017-06354-1
LEI, ZHIMIN ET AL.: "Chondrogenic Differentiation and Osteogenesis Gene Expression Following a Short-Time Induction of Adipose-Derived Stem Cells with Bone Morphogenetic Proteins", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH JUNE, vol. 13, no. 32, 6 August 2009 (2009-08-06), pages 6276 - 6278, XP009552076, ISSN: 2095-4344
PANJLA APURVA, QAYOOM IRFAN, KUMAR ASHOK, VERMA SANDEEP: "Anionic diketopiperazine induces osteogenic differentiation and supports osteogenesis in a 3D cryogel microenvironment", CHEMICAL COMMUNICATIONS, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 57, no. 60, 27 July 2021 (2021-07-27), UK , pages 7422 - 7425, XP093131137, ISSN: 1359-7345, DOI: 10.1039/D1CC01985F
Attorney, Agent or Firm:
MING & YUE INTELLECTUAL PROPERTY LAW FIRM (CN)
Download PDF: