Title:
APPLICATION OF PEPTIDYLARGININE DEIMINASE 2 IN PREPARATION OF REAGENT FOR CLINICAL DIAGNOSIS OF TUMORS
Document Type and Number:
WIPO Patent Application WO/2016/082445
Kind Code:
A1
Abstract:
An application of peptidylarginine deiminase 2 in preparation of a reagent or detection object for clinical diagnosis of tumors, wherein the tumors comprise liver cancers, gastric cancers, lung cancers, breast cancers, esophageal cancers, ovarian cancers, uterine cancers and colorectal cancers. In the specific application, an antibody against peptidylarginine deiminase 2 is prepared by a conventional method, and a qualitative or quantitative method and a matching kit for detecting peptidylarginine deiminase 2 are established. A blood diagnosis reagent for diagnosing tumors comprises a peptidylarginine deiminase 2 antibody, an HRP-IgG antibody and conventional reagents in the blood diagnosis reagent, wherein the conventional reagents in the blood diagnosis reagent comprise a carbonate buffer solution, a PBST lotion, a blocking solution, a developing solution and a stop solution. Experiments prove that PAD2 can be applied as a tumor blood marker, and PAD2 has feasibility as the tumor marker.
Inventors:
CHANG XIAOTIAN (CN)
XING YANQIU (CN)
MA FANG (CN)
YANG DONGXIA (CN)
XING YANQIU (CN)
MA FANG (CN)
YANG DONGXIA (CN)
Application Number:
PCT/CN2015/077354
Publication Date:
June 02, 2016
Filing Date:
April 24, 2015
Export Citation:
Assignee:
SHANDONG CHUANGXIN PHARMACEUTICAL RES AND DEV CO LTD (CN)
International Classes:
G01N33/577
Domestic Patent References:
| WO2002029103A2 | 2002-04-11 | |||
| WO2014023957A2 | 2014-02-13 | |||
| WO2014086365A1 | 2014-06-12 |
Foreign References:
| US20050181375A1 | 2005-08-18 | |||
| CN104360070A | 2015-02-18 |
Other References:
MCELWEE, J.L. ET AL.: "Identification of PADI2 as a Potential Breast Cancer Biomarker and Therapeutic Target", BMC CANCER, vol. 12, 30 October 2012 (2012-10-30), pages 500 - 516
FENG, DONGYUN ET AL.: "Citrullination Preferentially Proceeds in Glomerular Bowman's Capsule and Increases in Obstructive Nephropathy", KIDNEY INT., vol. 68, no. 1, 31 July 2005 (2005-07-31), pages 84 - 95
HARVEY, GP: "Expression of Peptidylarginine Deiminase-2 and-4, Citrullinated Proteins and Anti-Citrullinated Protein Antibodies in Human Gingiva", JOURNAL OF PERIODONTAL RESEARCH, vol. 48, no. 2, 16 September 2012 (2012-09-16), pages 252 - 261
FENG, DONGYUN ET AL.: "Citrullination Preferentially Proceeds in Glomerular Bowman's Capsule and Increases in Obstructive Nephropathy", KIDNEY INT., vol. 68, no. 1, 31 July 2005 (2005-07-31), pages 84 - 95
HARVEY, GP: "Expression of Peptidylarginine Deiminase-2 and-4, Citrullinated Proteins and Anti-Citrullinated Protein Antibodies in Human Gingiva", JOURNAL OF PERIODONTAL RESEARCH, vol. 48, no. 2, 16 September 2012 (2012-09-16), pages 252 - 261
Attorney, Agent or Firm:
JINAN LUKE PATENT AGENT CO., LTD. (CN)
济南鲁科专利代理有限公司 (CN)
济南鲁科专利代理有限公司 (CN)
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