Title:
APPLICATION OF POLYINOSINIC ACID AND SARS-COV-2 SPIKE PROTEIN IN CONSTRUCTION OF MOUSE ARDS MODEL
Document Type and Number:
WIPO Patent Application WO/2021/244235
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of biological medicine, and relates to an application of polyinosinic acid and SARS-CoV-2 spike protein in the construction of a mouse ARDS model. The model may be used for lung inflammation research and drug research and development. The preset invention verifies that in an animal model, polyinosinic acid and the SARS-CoV-2 spike protein may cause acute lung injury of a mouse, may simulate acute respiratory distress syndrome (ARDS) symptoms, and may effectively induce neutrophils to infiltrate into the lung and activate. Induction of polyinosinic acid and the SARS-CoV-2 spike protein induces tissue injury caused by the neutrophils, and an obvious inflammatory reaction induction effect exists. The polyinosinic acid and the SARS-CoV-2 spike protein may be further applied to research and development of a treatment drug for ARDS caused by viral pulmonary infection.
Inventors:
DONG ZIGANG (CN)
GU TINGXUAN (CN)
LIU KANGDONG (CN)
ZHAO SIMIN (CN)
GU TINGXUAN (CN)
LIU KANGDONG (CN)
ZHAO SIMIN (CN)
Application Number:
PCT/CN2021/093023
Publication Date:
December 09, 2021
Filing Date:
May 11, 2021
Export Citation:
Assignee:
CHINA US HENAN HORMEL CANCER INST (CN)
International Classes:
A61K38/16; A01K67/027; A61K31/713
Domestic Patent References:
| WO2019177991A1 | 2019-09-19 |
Foreign References:
| CN101745109A | 2010-06-23 | |||
| CN109355334A | 2019-02-19 |
Other References:
GU TINGXUAN, ZHAO SIMIN, JIN GUOGUO, SONG MENGQIU, ZHI YAFEI, ZHAO RAN, MA FAYANG, ZHENG YAQIU, WANG KEKE, LIU HUI, XIN MINGXIA, H: "Cytokine Signature Induced by SARS-CoV-2 Spike Protein in a Mouse Model", FRONTIERS IN IMMUNOLOGY, vol. 11, 28 January 2021 (2021-01-28), XP055877307, DOI: 10.3389/fimmu.2020.621441
GAN TINGTING, YANG YONGLIN, HU FAN, CHEN XICHEN, ZHOU JIAWEI, LI YAN, XU YING, WANG HUIJUAN, CHEN YU, ZHANG MINGSHUN: "TLR3 Regulated Poly I:C-Induced Neutrophil Extracellular Traps and Acute Lung Injury Partly Through p38 MAP Kinase", FRONTIERS IN MICROBIOLOGY, vol. 9, 1 January 2018 (2018-01-01), pages 3174, XP055877303, DOI: 10.3389/fmicb.2018.03174
MAHMUDPOUR MEHDI; ROOZBEH JAMSHID; KESHAVARZ MOHSEN; FARROKHI SHOKROLLAH; NABIPOUR IRAJ: "COVID-19 cytokine storm: The anger of inflammation", CYTOKINE, ACADEMIC PRESS LTD, PHILADELPHIA, PA, US, vol. 133, 30 May 2020 (2020-05-30), US , XP086227981, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2020.155151
JINCUN ZHAO, LI KUN, WOHLFORD-LENANE CHRISTINE, AGNIHOTHRAM SUDHAKAR S., FETT CRAIG, ZHAO JINGXIAN, GALE MICHAEL J., BARIC RALPH S: "Rapid generation of a mouse model for Middle East respiratory syndrome", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 13, 1 April 2014 (2014-04-01), pages 4970 - 4975, XP055212024, ISSN: 00278424, DOI: 10.1073/pnas.1323279111
COHEN JON: "From Mice to Monkeys, Animals Studied for Coronavirus Answers", SCIENCE, 17 April 2020 (2020-04-17), XP055877299
BARILE ELISA, BAGGIO CARLO, GAMBINI LUCA, SHIRYAEV SERGEY A., STRONGIN ALEX Y., PELLECCHIA MAURIZIO: "Potential Therapeutic Targeting of Coronavirus Spike Glycoprotein Priming", MOLECULES, vol. 25, no. 10, 22 May 2020 (2020-05-22), XP055877297, DOI: 10.3390/molecules25102424
Y KUMAKI ET AL.: "Prophylactic and Therapeutic Intranasal Administration with an Immunomodulator, Hiltonol® (Poly IC:LC), in a Lethal SARS-CoV-infected BALB/c Mouse Model", ANTIVIRAL RESEARCH, vol. 139, 9 December 2016 (2016-12-09), XP029901943, DOI: 10.1016/j.antiviral.2016.12.007
GAN TINGTING, YANG YONGLIN, HU FAN, CHEN XICHEN, ZHOU JIAWEI, LI YAN, XU YING, WANG HUIJUAN, CHEN YU, ZHANG MINGSHUN: "TLR3 Regulated Poly I:C-Induced Neutrophil Extracellular Traps and Acute Lung Injury Partly Through p38 MAP Kinase", FRONTIERS IN MICROBIOLOGY, vol. 9, 1 January 2018 (2018-01-01), pages 3174, XP055877303, DOI: 10.3389/fmicb.2018.03174
MAHMUDPOUR MEHDI; ROOZBEH JAMSHID; KESHAVARZ MOHSEN; FARROKHI SHOKROLLAH; NABIPOUR IRAJ: "COVID-19 cytokine storm: The anger of inflammation", CYTOKINE, ACADEMIC PRESS LTD, PHILADELPHIA, PA, US, vol. 133, 30 May 2020 (2020-05-30), US , XP086227981, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2020.155151
JINCUN ZHAO, LI KUN, WOHLFORD-LENANE CHRISTINE, AGNIHOTHRAM SUDHAKAR S., FETT CRAIG, ZHAO JINGXIAN, GALE MICHAEL J., BARIC RALPH S: "Rapid generation of a mouse model for Middle East respiratory syndrome", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 13, 1 April 2014 (2014-04-01), pages 4970 - 4975, XP055212024, ISSN: 00278424, DOI: 10.1073/pnas.1323279111
COHEN JON: "From Mice to Monkeys, Animals Studied for Coronavirus Answers", SCIENCE, 17 April 2020 (2020-04-17), XP055877299
BARILE ELISA, BAGGIO CARLO, GAMBINI LUCA, SHIRYAEV SERGEY A., STRONGIN ALEX Y., PELLECCHIA MAURIZIO: "Potential Therapeutic Targeting of Coronavirus Spike Glycoprotein Priming", MOLECULES, vol. 25, no. 10, 22 May 2020 (2020-05-22), XP055877297, DOI: 10.3390/molecules25102424
Y KUMAKI ET AL.: "Prophylactic and Therapeutic Intranasal Administration with an Immunomodulator, Hiltonol® (Poly IC:LC), in a Lethal SARS-CoV-infected BALB/c Mouse Model", ANTIVIRAL RESEARCH, vol. 139, 9 December 2016 (2016-12-09), XP029901943, DOI: 10.1016/j.antiviral.2016.12.007
Attorney, Agent or Firm:
ZHENGZHOU LIANKE PATENT AGENCY (COMMON PARTNERSHIP) (CN)
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