Title:
APTAMER SELECTION METHOD AND IMMUNITY ANALYSIS METHOD USING APTAMER
Document Type and Number:
WIPO Patent Application WO/2021/006570
Kind Code:
A1
Abstract:
The present invention relates to an aptamer selection method, and an aptamer selected by the selection method of the present invention is applicable in various fields such as sandwich-type biosensors, as the aptamer binds to an area of a target substance that is different from the area to which an antibody binds, and a significant amount of time can be saved, as no separate pairing selection process is needed. In addition, such aptamer has excellent stability and sensitivity compared to a conventional preparation comprising an antibody, can be mass produced at a low cost in a short amount of time via a chemical synthesis method, and is easily transformed in various ways to increase a binding force. An immunity analysis method using an aptamer, according to the present invention, detects a relative fluorescent signal by selectively amplifying only the aptamer bound to the target substance in a heterogeneous sandwich structure, and can, thereby, sensitively and quickly detect the target substance.
More Like This:
Inventors:
KIM MIN GON (KR)
KANG JU YOUNG (KR)
KANG JU YOUNG (KR)
Application Number:
PCT/KR2020/008759
Publication Date:
January 14, 2021
Filing Date:
July 03, 2020
Export Citation:
Assignee:
SB BIOSCIENCE CO LTD (KR)
International Classes:
C12N15/10; C12N15/115; C12Q1/68; G01N33/543
Foreign References:
| KR20170021424A | 2017-02-28 | |||
| KR20140015455A | 2014-02-06 | |||
| KR20100044787A | 2010-04-30 | |||
| KR20150030655A | 2015-03-20 | |||
| KR102113078B1 | 2020-05-20 | |||
| US7536428A | ||||
| US5475096A | 1995-12-12 | |||
| US5270163A | 1993-12-14 | |||
| US5789163A | 1998-08-04 |
Other References:
KANG JUYOUNG, YEOM GYUHO, HA SU-JI, KIM MIN-GON: "Development of a DNA aptamer selection method based on the heterogeneous sandwich form and its application in a colorimetric assay for influenza A virus detection", NEW JOURNAL OF CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 43, no. 18, 7 May 2019 (2019-05-07), GB, pages 6883 - 6889, XP055773588, ISSN: 1144-0546, DOI: 10.1039/C8NJ06458J
FISCHERNICHOLAS O ET AL.: "Protein detection via direct enzymatic amplification of short DNA aptamers", ANALYTICAL BIOCHEMISTRY, vol. 373, no. 1, 2008, pages 121 - 8
See also references of EP 3995575A4
FISCHERNICHOLAS O ET AL.: "Protein detection via direct enzymatic amplification of short DNA aptamers", ANALYTICAL BIOCHEMISTRY, vol. 373, no. 1, 2008, pages 121 - 8
See also references of EP 3995575A4
Attorney, Agent or Firm:
G1 IP LAW FIRM (KR)
Download PDF: