Title:
ARGINASE-ACTIVITY MEASURING METHOD, ARGINASE-ACTIVITY DETECTION KIT, AND ARGINASE-RELATED-DISEASE DETECTION KIT
Document Type and Number:
WIPO Patent Application WO/2017/085947
Kind Code:
A1
Abstract:
An arginase-activity measuring method according to the present invention is characterized in that the method includes: a step for activating arginase by adding a sample containing arginase and a solution containing divalent cation to a reaction vessel; a step for performing an enzyme-substrate reaction by adding an arginine solution to the sample containing the activated arginase; and a step for simultaneously performing enzyme deactivation, as well as urea-detection reaction, by adding an acidic solution that has a pH of 1.0-4.0 and that contains a urea-detection reagent to the sample in which the enzyme-substrate reaction has been performed.
Inventors:
MAENO EMI (JP)
Application Number:
PCT/JP2016/061637
Publication Date:
May 26, 2017
Filing Date:
March 31, 2016
Export Citation:
Assignee:
TOKYO OHKA KOGYO CO LTD (JP)
TABATA YASUHIKO (JP)
TABATA YASUHIKO (JP)
International Classes:
G01N33/50; C12Q1/34
Foreign References:
| JP2015516397A | 2015-06-11 | |||
| JP2010500973A | 2010-01-14 |
Other References:
ALMY, J ET AL.: "Urea Analysis for Wines", J. AGRIC. FOOD CHEM., vol. 37, 1989, pages 968 - 970, XP055488045
Attorney, Agent or Firm:
TANAI, Sumio et al. (JP)
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