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Title:
ARTIFICIAL BASE PAIR CAPABLE OF FORMING SPECIFIC BASE PAIR
Document Type and Number:
WIPO Patent Application WO/2011/043385
Kind Code:
A1
Abstract:
Disclosed is a double-stranded nucleic acid wherein at least one of the nucleic acids has an artificial base capable of forming a self-complementary base pair or an artificial base capable of forming a base pair of a heat stability at the same level with any natural base. Also disclosed are a method for hybridizing a first nucleic acid, which has an artificial base capable of forming a self-complementary base pair or an artificial base capable of forming a base pair of a heat stability at the same level with any natural base, with a second nucleic acid, and a method for using said first nucleic acid in detecting an SNP or applying the same to a DNA chip, DNA/RNA computing or an in vitro translation system. Also disclosed is a method for manipulating the thermodynamic stability in the hybridization of a nucleic acid by introducing an artificial base into said nucleic acid.

Inventors:
HIRAO, Ichiro (3-8-1-703, Iwadokita Komae-sh, Tokyo 04, 〒2010004, JP)
平尾 一郎 (〒04 東京都狛江市岩戸北3-8-1-703 Tokyo, 〒2010004, JP)
HIRAO, Michiko (Yokohama Institute 7-22, Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 〒2300045, JP)
Application Number:
JP2010/067560
Publication Date:
April 14, 2011
Filing Date:
October 06, 2010
Export Citation:
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Assignee:
RIKEN (2-1 Hirosawa, Wako-shi Saitama, 98, 〒3510198, JP)
独立行政法人理化学研究所 (〒98 埼玉県和光市広沢2番1号 Saitama, 〒3510198, JP)
TagCyx Biotechnologies (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 〒2300045, JP)
タグシクス・バイオ株式会社 (〒45 神奈川県横浜市鶴見区広末町1丁目7番22号 独立行政法人理化学研究所内連絡事務所 Kanagawa, 〒2300045, JP)
HIRAO, Ichiro (3-8-1-703, Iwadokita Komae-sh, Tokyo 04, 〒2010004, JP)
International Classes:
C07H21/02; C07H19/23; C07H19/24; C07H21/04; C12M1/00; C12N15/09; C12P21/02; C12Q1/68
Domestic Patent References:
WO2007066737A1
WO2005026187A1
WO2001005801A1
WO2007066737A1
WO2007066737A1
Foreign References:
US5432272A
US6140496A
US6617106B1
US6977161B2
Other References:
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FUJIWARA, T. ET AL.: "Synthesis of 6-(2-thienyl) purine nucleoside derivatives that form unnatural base pairs with pyridin-2-one nucleosides", BIOORG. MED. CHEM. LETT., vol. 11, no. 16, 2001, pages 2221 - 2223, XP002974103
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MITSUI, T. ET AL.: "Characterization of fluorescent, unnatural base pairs", TETRAHEDRON, vol. 63, 2007, pages 3528 - 3537, XP005929584
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Attorney, Agent or Firm:
ONO, Shinjiro et al. (YUASA AND HARA, Section 206 New Ohtemachi Bldg., 2-1, Ohtemachi 2-chome, Chiyoda-k, Tokyo 04, 〒1000004, JP)
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