Title:
ARTIFICIAL GENE AND GENE MUTATION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/029391
Kind Code:
A1
Abstract:
An artificial gene in which the abundance of 15N exceeds the natural abundance thereof in bases of at least a part of the DNA. A gene mutation method comprising: a first step for preparing a situation where 15N is eccentrically located in a specific DNA in a living cell; and a second step for irradiating a proton beam at such an energy that 15N causes a resonance nuclear reaction.
Inventors:
IWATA YASUSHI (JP)
TOMITA KANAKO (JP)
SUZUKI IWANE (JP)
MORIOKA RYO (JP)
YANG TIANJING (JP)
TOMITA KANAKO (JP)
SUZUKI IWANE (JP)
MORIOKA RYO (JP)
YANG TIANJING (JP)
Application Number:
PCT/JP2020/030476
Publication Date:
February 18, 2021
Filing Date:
August 07, 2020
Export Citation:
Assignee:
AIST (JP)
UNIV TSUKUBA (JP)
UNIV TSUKUBA (JP)
International Classes:
A01H1/06; C12N15/00
Foreign References:
| US6258567B1 | 2001-07-10 | |||
| US20150284783A1 | 2015-10-08 | |||
| JP2002502249A | 2002-01-22 | |||
| JP2018000129A | 2018-01-11 |
Other References:
LOUIS, JOHN M. ET AL.: "Preparation of Uniformly Isotope-labeled DNA Oligonucleotides for NMR Spectroscopy", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 4, 1998, pages 2374 - 2378, XP002166183, DOI: 10.1074/jbc.273.4.2374
BABER JAMES L., LEVENS DAVID, LIBUTTI DANIEL, TJANDRA NICO: "Chemical Shift Mapped DNA-Binding Sites and 15N Relaxation Analysis of the C-Terminal KH Domain of Heterogeneous Nuclear Ribonucleoprotein K", BIOCHEMISTRY, vol. 39, 2000, pages 6022 - 6032, XP055793326, DOI: 10.1021/bi000105e
GROMIHA, MICHAEL M.: "Influence of DNA stiffness in protein-DNA recognition", JOURNAL OF BIOTECHNOLOGY, vol. 117, 2005, pages 137 - 145, XP027663425, DOI: 10.1016/j.jbiotec. 2004.12.01 6
FUKUTANI, KATSUYUKI; MURATA, YOSHITADA: "Fundamentals and Present Aspects of Ion Beam Technology. V. Application of Ion Beam 1. Application to Physical Sciences. 1.2 Surface hydrogen detection", RADIOISOTOPES, vol. 44, no. 7, 15 July 1995 (1995-07-15), JP , pages 486 - 489, XP009534761, ISSN: 0033-8303, DOI: 10.3769/radioisotopes.44.7_486
HASE, YOSHIHIRO: "Characteristics and Industrial Application of Ion Beam Breeding Technique", KAGAKU TO SEIBUTSU , vol. 52, no. 10, 30 November 2013 (2013-11-30), JP , pages 659 - 664, XP009534494, ISSN: 0453-073X, DOI: 10.1271/kagakutoseibutsu.52.659
YUSUKE KAZAMA, TOMONARI HIRANO, SHUHEI OTA, YOICHI SATO, KOJI MURAI, SHIGEYUKI KAWANO, KATSUNORI ONO, TOMOKO ABE: "Green Innovation via Heavy-Ion Breading", JOURNAL OF CROP RESEARCH, vol. 61, 2016, pages 73 - 79, XP055793332
BABER JAMES L., LEVENS DAVID, LIBUTTI DANIEL, TJANDRA NICO: "Chemical Shift Mapped DNA-Binding Sites and 15N Relaxation Analysis of the C-Terminal KH Domain of Heterogeneous Nuclear Ribonucleoprotein K", BIOCHEMISTRY, vol. 39, 2000, pages 6022 - 6032, XP055793326, DOI: 10.1021/bi000105e
GROMIHA, MICHAEL M.: "Influence of DNA stiffness in protein-DNA recognition", JOURNAL OF BIOTECHNOLOGY, vol. 117, 2005, pages 137 - 145, XP027663425, DOI: 10.1016/j.jbiotec. 2004.12.01 6
FUKUTANI, KATSUYUKI; MURATA, YOSHITADA: "Fundamentals and Present Aspects of Ion Beam Technology. V. Application of Ion Beam 1. Application to Physical Sciences. 1.2 Surface hydrogen detection", RADIOISOTOPES, vol. 44, no. 7, 15 July 1995 (1995-07-15), JP , pages 486 - 489, XP009534761, ISSN: 0033-8303, DOI: 10.3769/radioisotopes.44.7_486
HASE, YOSHIHIRO: "Characteristics and Industrial Application of Ion Beam Breeding Technique", KAGAKU TO SEIBUTSU , vol. 52, no. 10, 30 November 2013 (2013-11-30), JP , pages 659 - 664, XP009534494, ISSN: 0453-073X, DOI: 10.1271/kagakutoseibutsu.52.659
YUSUKE KAZAMA, TOMONARI HIRANO, SHUHEI OTA, YOICHI SATO, KOJI MURAI, SHIGEYUKI KAWANO, KATSUNORI ONO, TOMOKO ABE: "Green Innovation via Heavy-Ion Breading", JOURNAL OF CROP RESEARCH, vol. 61, 2016, pages 73 - 79, XP055793332
Attorney, Agent or Firm:
INABA, Yoshiyuki et al. (JP)
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