Title:
ARTIFICIAL RNA RESTRICTION ENZYME
Document Type and Number:
WIPO Patent Application WO/2017/104796
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing an artificial RNA restriction enzyme with which it is possible to disable an RNA virus such as an influenza virus by cleavage. The invention is an artificial RNA restriction enzyme formed by linking an RNA-binding protein that binds with RNA and an enzyme that cleaves a predetermined site of the RNA, via a peptide linker. The invention is also characterized in that the length of the peptide linker is five amino acids when the enzyme linked via the peptide linker is an SNase and the length of the peptide linker is five amino acids or ten amino acids when the enzyme linked via the peptide linker is a PIN.
Inventors:
SERA TAKASHI (JP)
Application Number:
PCT/JP2016/087538
Publication Date:
June 22, 2017
Filing Date:
December 16, 2016
Export Citation:
Assignee:
NAT UNIV CORP OKAYAMA UNIV (JP)
International Classes:
C12N9/22; A61K38/00; A61K38/43; A61K47/42; A61K47/50; C12N15/09; A61P31/14
Domestic Patent References:
| WO2015163037A1 | 2015-10-29 | |||
| WO2014180754A1 | 2014-11-13 |
Foreign References:
| JP2008540342A | 2008-11-20 | |||
| JP2009528816A | 2009-08-13 |
Other References:
CHOUDHURY, R. ET AL.: "Engineering RNA endonucleases with customized sequence specificities", NATURE COMMUNICATIONS, vol. 3, no. 1, 2012, XP055499173
WANG, C. C. ET AL.: "Inhibitor Binding Increases the Mechanical Stability of Staphylococcal Nuclease", BIOPHYSICAL JOURNAL, vol. 100, no. 4, 2011, pages 1094 - 1099, XP028361310, DOI: doi:10.1016/j.bpj.2011.01.011
ARCUS, V. L. ET AL.: "The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array,", PROTEIN ENGINEERING DESIGN & SELECTION, vol. 24, no. 1-2, 2011, pages 33 - 40, XP055598814
MORI, T. ET AL.: "Cleavage of influenza RNA by using a human PUF-based artificial RNA-binding protein -staphylococcal nuclease hybrid", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 479, no. 4, 28 September 2016 (2016-09-28), pages 736 - 740
MELEKHOVETS, Y. F. ET AL.: "Fusion with an RNA binding domain to confer target RNA specificity to an RNase: design and engineering of Tat-RNase H that specifically recognizes and cleaves HIV-1 RNA in vitro", NUCLEIC ACIDS RESEARCH, vol. 24, no. 10, 1996, pages 1908 - 1912, XP002904225, DOI: doi:10.1093/nar/24.10.1908
WANG, C. C. ET AL.: "Inhibitor Binding Increases the Mechanical Stability of Staphylococcal Nuclease", BIOPHYSICAL JOURNAL, vol. 100, no. 4, 2011, pages 1094 - 1099, XP028361310, DOI: doi:10.1016/j.bpj.2011.01.011
ARCUS, V. L. ET AL.: "The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array,", PROTEIN ENGINEERING DESIGN & SELECTION, vol. 24, no. 1-2, 2011, pages 33 - 40, XP055598814
MORI, T. ET AL.: "Cleavage of influenza RNA by using a human PUF-based artificial RNA-binding protein -staphylococcal nuclease hybrid", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 479, no. 4, 28 September 2016 (2016-09-28), pages 736 - 740
MELEKHOVETS, Y. F. ET AL.: "Fusion with an RNA binding domain to confer target RNA specificity to an RNase: design and engineering of Tat-RNase H that specifically recognizes and cleaves HIV-1 RNA in vitro", NUCLEIC ACIDS RESEARCH, vol. 24, no. 10, 1996, pages 1908 - 1912, XP002904225, DOI: doi:10.1093/nar/24.10.1908
Attorney, Agent or Firm:
SIKS & CO. (JP)
Download PDF:
Previous Patent: SLIDE BEARING
Next Patent: RADICAL GENERATING CATALYST, METHOD FOR PRODUCING RADICAL, METHOD FOR PRODUCING OXIDATION REACTION P...
Next Patent: RADICAL GENERATING CATALYST, METHOD FOR PRODUCING RADICAL, METHOD FOR PRODUCING OXIDATION REACTION P...