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Title:
ASSAY METHOD AND ASSAY KIT FOR HEPATITIS B VIRUS S ANTIGEN
Document Type and Number:
WIPO Patent Application WO/2019/107279
Kind Code:
A1
Abstract:
Disclosed are a high-sensitivity HBsAg assay method and assay kit that do not involve a strong acid or alkaline treatment during sample pretreatment and are not readily affected by autoantibodies. An assay method for the hepatitis B virus s antigen in a sample that has been isolated from an organism, the method including: a pretreatment step for mixing the sample with a pretreatment reagent that includes a reducing agent to reduce the hepatitis B virus s antigen; and an immunoassay step for performing an immunoassay for hepatitis B virus s antigen on the pretreated sample using an antibody that can achieve an antigen-antibody interaction with a reduced peptide that comprises the 98th–179th amino acids of hepatitis B virus s antigen and/or an antigen-binding fragment of the antibody.

Inventors:
OHUE CHIHARU (JP)
IIDA KUMIKO (JP)
AOYAGI KATSUMI (JP)
YAGI SHINTARO (JP)
Application Number:
PCT/JP2018/043229
Publication Date:
June 06, 2019
Filing Date:
November 22, 2018
Export Citation:
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Assignee:
FUJIREBIO KK (JP)
International Classes:
G01N33/576; C07K14/02
Domestic Patent References:
WO2008053901A12008-05-08
WO2014115878A12014-07-31
Foreign References:
JP4430677B22010-03-10
JP4430677B22010-03-10
EP2088431A12009-08-12
JPS6026564B21985-06-24
Other References:
V. D. SIEGLERVOLKER BRUSS: "Role of Transmembrane Domains of Hepatitis B Virus Small Surface Proteins in Subviral-Particle Biogenesis", J. VIROLOGY, vol. 87, no. 3, 2013, pages 1491 - 1496, XP055106308, DOI: 10.1128/JVI.02500-12
HIROAKI OKAMOTO: "Molecular Hepatitis Virology-Fundamental, Clinical, and Prophylactic Aspects. Hepatitis A, B, D, and E Viruses", JAPANESE JOURNAL OF CLINICAL MEDICINE, 26 October 1995 (1995-10-26), pages 212 - 222
"New Biochemistry Experiments Lecture"
See also references of EP 3719499A4
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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