ASSAY OF MICRO-ORGANISMS IN CELL CULTURES

Title:

ASSAY OF MICRO-ORGANISMS IN CELL CULTURES

Document Type and Number:

WIPO Patent Application WO2001034830

Kind Code:

A3

Abstract:

Micro-organisms which over-produce ATPase, especially mycoplasmas in a sample of a cell culture, are detected by making use of the ATPase activity to convert cellular or externally added ATP to ADP. The decrease in ATP externally added or the increase or absolute level of ADP produced from cellular ATP can then be detected or measured. For example, if a reagent such as phosphenol pyruvate/pyruvate kinase is added to convert that ADP back to ATP, the resulting high level of ATP can be detected bioluminescently using the reaction: Luciferin + luciferase enzyme + ATP } h upsilon + products.

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Inventors:

CROUCH SHARON PATRICIA MARY (GB)
BRADBURY DAWN ANN (GB)
SLATER KEVIN JOHN (GB)

Application Number:

PCT/GB2000/004264

Publication Date:

October 11, 2001

Filing Date:

November 08, 2000

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Assignee:

LUMITECH UK LTD (GB)
CROUCH SHARON PATRICIA MARY (GB)
BRADBURY DAWN ANN (GB)
SLATER KEVIN JOHN (GB)

International Classes:

G01N21/78; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/48; C12Q1/66; C12R1/35; (IPC1-7): C12Q1/04; C12Q1/66

Foreign References:

GB2323167A

1998-09-16

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