Title:
ASSAYS AND METHODS TO SEQUENCE MICROBES DIRECTLY FROM IMMUNE COMPLEXES
Document Type and Number:
WIPO Patent Application WO/2012/103337
Kind Code:
A3
Abstract:
Provided herein are MiIP-Seq assays and methods for detecting and/or identifying an immune-stimulating microbe in a patient sample. Also provided herein are methods for diagnosing an infectious disease or identifying a previously uncharacterized microbe in a patient sample. The methods and assays described herein are advantageous over existing methods in that they (i) do not require a culture step for microbe expansion, (ii) are not specific for a particular microbe and can be used to identify a previously uncharacterized microbe, and (iii) permits rapid processing due to the lack of a microbe culture step.
Inventors:
BLUMBERG RICHARD (US)
KASER ARTHUR (GB)
KASER ARTHUR (GB)
Application Number:
PCT/US2012/022727
Publication Date:
November 22, 2012
Filing Date:
January 26, 2012
Export Citation:
Assignee:
BRIGHAM & WOMENS HOSPITAL (US)
BLUMBERG RICHARD (US)
KASER ARTHUR (GB)
UNIV INNSBRUCK (AT)
BLUMBERG RICHARD (US)
KASER ARTHUR (GB)
UNIV INNSBRUCK (AT)
International Classes:
C12Q1/68; C07K1/14; C12Q1/04; G01N33/569; G01N33/68
Foreign References:
| US20090111102A1 | 2009-04-30 |
Other References:
URBACH, E. ET AL.: "Immunochemical detection and isolation of DNA from metabolically active bacteria", APPL. ENVIRON. MICROBIOL., vol. 65, no. 3, March 1999 (1999-03-01), pages 1207 - 1213
FRANK, D. N. ET AL.: "Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases", PNAS U. S. A., vol. 104, no. 34, 21 August 2007 (2007-08-21), pages 13780 - 13785, XP055050956, DOI: doi:10.1073/pnas.0706625104
PETERSON, D. A. ET AL.: "Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseases", CELL HOST MICROBE., vol. 3, no. 6, 12 June 2008 (2008-06-12), pages 417 - 427
FRANK, D. N. ET AL.: "Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases", PNAS U. S. A., vol. 104, no. 34, 21 August 2007 (2007-08-21), pages 13780 - 13785, XP055050956, DOI: doi:10.1073/pnas.0706625104
PETERSON, D. A. ET AL.: "Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseases", CELL HOST MICROBE., vol. 3, no. 6, 12 June 2008 (2008-06-12), pages 417 - 427
Attorney, Agent or Firm:
EISENSTEIN, Ronald, I. et al. (Nixon Peabody LLPBoston, MA, US)
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