Title:
ASYMMETRIC CELL DIVISION INHIBITOR, CHROMOSOMAL ANEUPLOIDY INHIBITOR, MEDIUM, IN VITRO FERTILIZATION MEDIUM, CELL TREATMENT KIT, ASYMMETRIC CELL DIVISION INHIBITION METHOD, IN VITRO FERTILIZATION METHOD, TRANSPLANTATION METHOD, INFERTILITY TREATMENT METHOD, AND OFFSPRING ACQUISITION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/100925
Kind Code:
A1
Abstract:
Provided is an asymmetric cell division inhibitor with which infertility due to aging or the like can be ameliorated. The asymmetric cell division inhibitor includes a spontaneous activation inhibitor that inhibits asymmetric cell division caused by the spontaneous activation of cells having pluripotency. When the cells are reproductive cells or ova, spontaneous activation occurs due to aging or exposure to carbon dioxide before fertilization. The spontaneous activation is accompanied by the dispersion of chromosomes. Therefore, the asymmetric cell division inhibitor inhibits asymmetric cell division by inducing the inhibition of spontaneous activation and/or the reaggregation of dispersed chromosomes. Specifically, the spontaneous activation inhibitor may be a proteasome inhibitor, and includes MG132 or ALLN. The treatment in an in vitro fertilization medium containing the collected asymmetric cell division inhibitor can inhibit the asymmetric cell division of reproductive cells or fertilized ova.
Inventors:
HONDA ARATA (JP)
Application Number:
PCT/JP2022/044127
Publication Date:
June 08, 2023
Filing Date:
November 30, 2022
Export Citation:
Assignee:
UNIV JICHI MEDICAL (JP)
International Classes:
C12N5/073; A01K67/02; A61K38/06; A61K45/00; A61P15/00; A61P43/00; C07K5/08; C12N5/074
Other References:
NAKAJIMA NORIAKI, INOMATA TOMO, ITO JUNYA, KASHIWAZAKI NAOMI: "Treatment with Proteasome Inhibitor MG132 during Cloning Improves Survival and Pronuclear Number of Reconstructed Rat Embryos", CLONING AND STEM CELLS, MARY ANN LIEBERT, LARCHMONT, US, vol. 10, no. 4, 1 December 2008 (2008-12-01), US , pages 461 - 468, XP093070025, ISSN: 1536-2302, DOI: 10.1089/clo.2008.0038
QI ZHOU, ET AL.: "Generation of Fertile Cloned Rats by Regulating Oocyte Activation", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 302, 14 November 2003 (2003-11-14), US , pages 1179, XP002277914, ISSN: 0036-8075, DOI: 10.1126/science.1088313
YOU JINYOUNG, LEE EUNSONG, BONILLA LUCIANO, FRANCIS JASMINE, KOH JIN, BLOCK JEREMY, CHEN SIXUE, HANSEN PETER J.: "Treatment with the Proteasome Inhibitor MG132 during the End of Oocyte Maturation Improves Oocyte Competence for Development after Fertilization in Cattle", PLOS ONE, vol. 7, no. 11, pages e48613, XP093070029, DOI: 10.1371/journal.pone.0048613
YOU JINYOUNG, KIM JINYOUNG, LEE HEOWWON, HYUN SANG-HWAN, HANSEN PETER J., LEE EUNSONG: "MG132 treatment during oocyte maturation improves embryonic development after somatic cell nuclear transfer and alters oocyte and embryo transcript abundance in pigs", MOLECULAR REPRODUCTION AND DEVELOPMENT, WILEY-LISS, INC, NEW YORK, NY, US, vol. 79, no. 1, 1 January 2012 (2012-01-01), NEW YORK, NY, US , pages 41 - 50, XP093070039, ISSN: 1040-452X, DOI: 10.1002/mrd.21402
XIAO XIAO;XIANG-DONG ZI;HUI-RAN NIU;XIAN-RONG XIONG;JIN-CHENG ZHONG;JIAN LI;LI WANG;YONG WANG: "Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes", REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, BIOMED CENTRAL LTD., GB, vol. 12, no. 1, 22 April 2014 (2014-04-22), GB , pages 30, XP021182004, ISSN: 1477-7827, DOI: 10.1186/1477-7827-12-30
TAN, X. ET AL.: "Participation of the ubiquitin-proteasome pathway in rat oocyte activation", ZYGOTE, vol. 13, no. 1, 2005, pages 87 - 95, XP009546770
TETSUO ONO; EIJI MIZUTANI; CHONG LI; KAZUO YAMAGATA; TERUHIKO WAKAYAMA: "Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132", GENESIS, WILEY-LISS, HOBOKEN, USA, vol. 49, no. 6, 31 May 2011 (2011-05-31), Hoboken, USA, pages 460 - 471, XP072303681, ISSN: 1526-954X, DOI: 10.1002/dvg.20756
QI ZHOU, ET AL.: "Generation of Fertile Cloned Rats by Regulating Oocyte Activation", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 302, 14 November 2003 (2003-11-14), US , pages 1179, XP002277914, ISSN: 0036-8075, DOI: 10.1126/science.1088313
YOU JINYOUNG, LEE EUNSONG, BONILLA LUCIANO, FRANCIS JASMINE, KOH JIN, BLOCK JEREMY, CHEN SIXUE, HANSEN PETER J.: "Treatment with the Proteasome Inhibitor MG132 during the End of Oocyte Maturation Improves Oocyte Competence for Development after Fertilization in Cattle", PLOS ONE, vol. 7, no. 11, pages e48613, XP093070029, DOI: 10.1371/journal.pone.0048613
YOU JINYOUNG, KIM JINYOUNG, LEE HEOWWON, HYUN SANG-HWAN, HANSEN PETER J., LEE EUNSONG: "MG132 treatment during oocyte maturation improves embryonic development after somatic cell nuclear transfer and alters oocyte and embryo transcript abundance in pigs", MOLECULAR REPRODUCTION AND DEVELOPMENT, WILEY-LISS, INC, NEW YORK, NY, US, vol. 79, no. 1, 1 January 2012 (2012-01-01), NEW YORK, NY, US , pages 41 - 50, XP093070039, ISSN: 1040-452X, DOI: 10.1002/mrd.21402
XIAO XIAO;XIANG-DONG ZI;HUI-RAN NIU;XIAN-RONG XIONG;JIN-CHENG ZHONG;JIAN LI;LI WANG;YONG WANG: "Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes", REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, BIOMED CENTRAL LTD., GB, vol. 12, no. 1, 22 April 2014 (2014-04-22), GB , pages 30, XP021182004, ISSN: 1477-7827, DOI: 10.1186/1477-7827-12-30
TAN, X. ET AL.: "Participation of the ubiquitin-proteasome pathway in rat oocyte activation", ZYGOTE, vol. 13, no. 1, 2005, pages 87 - 95, XP009546770
TETSUO ONO; EIJI MIZUTANI; CHONG LI; KAZUO YAMAGATA; TERUHIKO WAKAYAMA: "Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132", GENESIS, WILEY-LISS, HOBOKEN, USA, vol. 49, no. 6, 31 May 2011 (2011-05-31), Hoboken, USA, pages 460 - 471, XP072303681, ISSN: 1526-954X, DOI: 10.1002/dvg.20756
Attorney, Agent or Firm:
HORI, Shiroyuki et al. (JP)
Download PDF: