Title:
ATP-VISUALIZING ANIMAL AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/108102
Kind Code:
A1
Abstract:
A non-human mammal capable of expressing a fusion protein in which two fluorescent proteins are bound respectively on an amino terminal side and a carboxy terminal side of an ε-subunit of an ATP synthetase, wherein the two fluorescent proteins can respectively serve as a donor and an accepter in fluorescence resonance energy transfer; and a method for screening for a medicinal agent, such as a prophylactic/therapeutic agent for various diseases, using the non-human mammal.
Inventors:
YAMAMOTO MASAMICHI (JP)
Application Number:
PCT/JP2015/050932
Publication Date:
July 23, 2015
Filing Date:
January 15, 2015
Export Citation:
Assignee:
UNIV GUNMA NAT UNIV CORP (JP)
International Classes:
A01K67/027; C12Q1/02; G01N33/15; G01N33/50; C12N15/00; C12N15/09
Domestic Patent References:
| WO2008117792A1 | 2008-10-02 |
Other References:
THUNEMANN, M. ET AL.: "Transgenic mice for cGMP imaging.", CIRCULATION RESEARCH, vol. 113, no. 4, 2013, pages 365 - 371, XP055212985
ZHANG, J. ET AL.: "In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice.", AM. J. PHYSIOL. HEART CIRC. PHYSIOL., vol. 299, no. 3, 1 September 2010 (2010-09-01), pages H946 - H956, XP055212993
AOKI, K. ET AL.: "Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: basis of biosensor construction, live imaging, and image processing.", DEV. GROWTH DIFFER., vol. 55, no. 4, 2013, pages 515 - 522, XP055213297
ZHANG, J. ET AL.: "In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice.", AM. J. PHYSIOL. HEART CIRC. PHYSIOL., vol. 299, no. 3, 1 September 2010 (2010-09-01), pages H946 - H956, XP055212993
AOKI, K. ET AL.: "Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: basis of biosensor construction, live imaging, and image processing.", DEV. GROWTH DIFFER., vol. 55, no. 4, 2013, pages 515 - 522, XP055213297
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
Yoshiyuki Kawaguchi (JP)
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