Title:
ATTENUATED SALMONELLA CARRYING EFFECTIVE PLASMID AND ITS ANTITUMORIGENIC USE
Document Type and Number:
WIPO Patent Application WO/2008/014668
Kind Code:
A1
Abstract:
The present invention relates to attenuated salmonella and its use in treating
tumors. The present invention belongs to biological technique field. The present
invention discloses the constructions of pH1Si-Stat3, pGRIM-19, and pGRIM-19-Si-Stat3
recombinant plasmid, which coexpresses GRIM-19 and siRNA-Stat3. An attenuation
salmonella electrotransformated with the recombinant plasmid is disclosed.
The coexpression of GRIM-19 and siRNA-Stat3 has a good synergistic inhibition
effect on the growth and metastases of tumors in the experiment in vivo. Attenuated
salmonella has phagorytosis effect on tumors, and the progenitive specificity
in tumortissue is more than 1000 times as that in normal tissue. The attenuated
salmonella carrying effective plasmid, which can coexpresses siRNA-Stat3
and GRIM-19, can reach the deep part of tumors, and thus markedly inhibit their
growth and metastases and evidently prolong the survival time of mice.
Inventors:
ZHAO, Xuejian (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
赵雪俭 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHANG, Ling (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
张灵 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
赵雪俭 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHANG, Ling (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
张灵 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
Application Number:
CN2007/002157
Publication Date:
February 07, 2008
Filing Date:
July 13, 2007
Export Citation:
Assignee:
JILIN UNIVERSITY (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
吉林大学 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHAO, Xuejian (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
赵雪俭 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHANG, Ling (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
吉林大学 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHAO, Xuejian (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
赵雪俭 (中国吉林省长春市新民大街2号, Jilin 1, 130021, CN)
ZHANG, Ling (No 2 Xinmin St. Changchun, Jilin 1, 130021, CN)
International Classes:
C12N1/21; A61K35/74; A61K38/17; A61K48/00; A61P35/00; C12N15/11; C12N15/113; C12N15/74; C12N15/12; C12R1/42; C12N1/21; A61K35/66; A61K38/17; A61K48/00; A61P35/00; C12N15/11; C12N15/113; C12N15/74; C12N15/12
Attorney, Agent or Firm:
JILINCHANGCHUN NEW CENTURY PATENT AGENT LTD. (No. 132, Renmin StreetChangchun, Jilin, Jilin, CN)
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