Title:
AUTOMATED NUCLEIC ACID SAMPLE PREPARATION, DETECTION, AND ANALYSIS SYSTEM
Document Type and Number:
WIPO Patent Application WO/2019/071450
Kind Code:
A1
Abstract:
Described herein is an integrated automated nucleic acid sample preparation, detection and analysis system, along with methods of operating such a system. Also described are methods of methods of analyzing nucleic acid molecules in a sample and methods ofdetermining a melting curve of a nucleic acid sample. The automated system includes a robotic pipettor comprising one or more pipettes movable in a horizontal plane and configured to dispense or withdraw one or more liquids； a robotic arm configured to transport a plurality of connected sample processing tubes； a nucleic acid isolation system comprising a first sample processing tube holder configured to hold the plurality of connected sample processing tubes, and magnet； wherein magnetically-responsive particles contained within each sample processing tube are retained within the sample processing tube upon withdrawing of liquid by the robotic pipettor when the magnet is in an active configuration； and a fluorometer comprising a light source and an optical detector disposed below a second sample processing tube holder configured to hold the plurality of connected sample processing tubes, the second sample processing tube holder having a clear or open bottom, wherein the fluorometer is configured to detect fluorescence emitted from a sample in one or more of the sample processing tubes.
More Like This:
Inventors:
JU JINLIANG (CN)
ZHENG HANWU (CN)
MAO YACHEN (CN)
JIN HAO (CN)
CHEN YI (CN)
ZHENG HANWU (CN)
MAO YACHEN (CN)
JIN HAO (CN)
CHEN YI (CN)
Application Number:
PCT/CN2017/105631
Publication Date:
April 18, 2019
Filing Date:
October 11, 2017
Export Citation:
Assignee:
SHANGHAI RENDU BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/68; C12M1/34; G01N33/533
Foreign References:
| US20060204997A1 | 2006-09-14 | |||
| CN103173434A | 2013-06-26 | |||
| CN203244989U | 2013-10-23 | |||
| CN101869855A | 2010-10-27 | |||
| US20120052560A1 | 2012-03-01 |
Other References:
WANG, L. Q. ET AL.: "Research progress of PCR decontamination methods", PROGRESS IN MODERN BIOMEDICINE, vol. 14, no. 11, 31 July 2011 (2011-07-31), pages 2797 - 2800
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
Download PDF: