AUTOMATIC CULTURE DEVICE

Title:

AUTOMATIC CULTURE DEVICE

Document Type and Number:

WIPO Patent Application WO/2012/020458

Kind Code:

A1

Abstract:

In order to uniformly sow cells on the culture surface of a cartridge-type closed culture container and remove bubbles contaminating a liquid culture medium in the course of automatic culture, an automatic culture device is provided with a movement-controlling mechanism section for solving the non-uniformity in cell distribution by, after filling up a culture space in the cartridge-type closed culture container, said cartridge-type closed culture container being in an upright position, with a cell suspension, turning the cartridge-type closed culture container into a horizontal position, and then repeatedly supplying the cell suspension and sucking the same multiple times to thereby create a mixing flow in the cell suspension. In this process, the liquid in channels is efficiently sent by applying a reduced pressure and an elevated pressure respectively to a channel on one side and a channel on the opposite side, using two syringes and check valves connected to the channels, to thereby load forces to the liquid from both sides. The liquid can be sent without any cell loss by conducting the same operations of the movement-controlling mechanism section in a tank and in a cell bag.

Inventors:

NOZAKI TAKAYUKI (JP)
KOBAYASHI TOYOSHIGE (JP)
MATSUOKA SHIZU (JP)
NAKAJIMA RYOTA (JP)

Application Number:

PCT/JP2010/005056

Publication Date:

February 16, 2012

Filing Date:

August 12, 2010

Export Citation:

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Assignee:

HITACHI LTD (JP)
NOZAKI TAKAYUKI (JP)
KOBAYASHI TOYOSHIGE (JP)
MATSUOKA SHIZU (JP)
NAKAJIMA RYOTA (JP)

International Classes:

C12M3/00

Foreign References:

JP2010104299A	2010-05-13
JP2004129568A	2004-04-30
JP2004208665A	2004-07-29
JP2008113600A	2008-05-22
JP2004089138A	2004-03-25
JP2006320304A	2006-11-30
JP2006149237A	2006-06-15
JP2006141328A	2006-06-08
JP2004208665A	2004-07-29

Other References: