Title:
AUTOMATIC ELECTROSPRAY SPRAYING APPARATUS AND METHOD FOR PREPARING MASS SPECTRUM IMAGING SAMPLE
Document Type and Number:
WIPO Patent Application WO/2023/193483
Kind Code:
A1
Abstract:
Disclosed in the present invention are an automatic electrospray spraying apparatus and method for preparing a mass spectrum imaging sample. The automatic electrospray spraying apparatus for preparing a mass spectrum imaging sample comprises: a spray capillary; an injection mechanism, an outlet end of which communicates with an inlet end of the spray capillary; a high-voltage power source, which is electrically connected to the spray capillary; and a mechanical arm, which is used for driving the spray capillary to move. In the present solution, micron-sized tiny fogdrops are generated by means of the electrospray principle, such that the spraying of a derivatization reagent and a matrix can be realized; and compared with the prior art, the present solution has an order-of-magnitude improvement in the degree of fineness of liquid droplets, it is guaranteed that a derivatization reaction does not destroy in-situ information, and it is also guaranteed that crystal grain of matrix crystallization have a small size and uniform distribution, thereby ensuring the mass spectrum imaging quality. In addition, in the present solution, a mechanical arm is further used for operating a spraying process, such that the spraying process is more flexible, the efficiency is higher, and the reagent can be automatically sucked and replaced, thereby realizing full-automatic sample preparation for multi-reagent spraying, such as superposed spraying of the derivatization reagent and the matrix.
Inventors:
LUO QIAN (CN)
DENG KA (CN)
LV YUEGUANG (CN)
DENG KA (CN)
LV YUEGUANG (CN)
Application Number:
PCT/CN2022/142308
Publication Date:
October 12, 2023
Filing Date:
December 27, 2022
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
H01J49/26; H01J49/16
Foreign References:
| CN114724921A | 2022-07-08 | |||
| CN106404885A | 2017-02-15 | |||
| CN204842003U | 2015-12-09 | |||
| CN111257406A | 2020-06-09 | |||
| CN106683973A | 2017-05-17 |
Other References:
YANFENG CHEN, JEREMY ALLEGOOD, YING LIU, ELAINE WANG, BEGOÑA CACHÓN-GONZÁLEZ, TIMOTHY M. COX, ALFRED H. MERRILL,, M. CAMERON SULLA: "Imaging MALDI Mass Spectrometry Using an Oscillating Capillary Nebulizer Matrix Coating System and Its Application to Analysis of Lipids in Brain from a Mouse Model of Tay−Sachs/Sandhoff Disease", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 80, no. 8, 1 April 2008 (2008-04-01), US , pages 2780 - 2788, XP055284276, ISSN: 0003-2700, DOI: 10.1021/ac702350g
Attorney, Agent or Firm:
PURPLEVINE INTELLECTUAL PROPERTY (SHENZHEN) CO., LTD. (CN)
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