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Title:
AVIAN MYELOBLASTOSIS VIRUS REVERSE TRANSCRIPTASE WITH IMPROVED HEAT STABILITY
Document Type and Number:
WIPO Patent Application WO/2014/104094
Kind Code:
A1
Abstract:
Provided are avian myeoblastosis virus (AMV) reverse transcriptase with improved heat stability and a method for producing the same. The AMV reverse transcriptase with improved heat stability can be obtained by substituting an amino acid at a specific position, among the amino acids constituting AMV reverse transcriptase, by another amino acid. The aforesaid reverse transcriptase can be produced by transforming a host with an expression vector containing a polynucleotide that encodes the reverse transcriptase and then culturing the obtained transformant.

Inventors:
SATO HIROSHI (JP)
NOGUCHI ATSUSHI (JP)
NAKANISHI MUTSUMI (JP)
MAKINO YURIKO (JP)
ICHII TAKASHI (JP)
IDE TERUHIKO (JP)
Application Number:
PCT/JP2013/084662
Publication Date:
July 03, 2014
Filing Date:
December 25, 2013
Export Citation:
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Assignee:
TOSOH CORP (JP)
International Classes:
C12N15/09; C12N1/21; C12N9/12; C12Q1/48; C12R1/19
Domestic Patent References:
WO2010016621A12010-02-11
Foreign References:
JP2008500818A2008-01-17
JP2002537817A2002-11-12
JP2005538719A2005-12-22
JP2004511211A2004-04-15
JP2011051943A2011-03-17
JP2013146235A2013-08-01
JP2013126402A2013-06-27
Other References:
KONISHI, A. ET AL.: "Improving the thermal stability of avian myeloblastosis virus reverse transcriptase a-subunit by site-directed mutagenesis", BIOTECHNOL. LETT., vol. 34, March 2012 (2012-03-01), pages 1209 - 1215
JONES, A. ET AL.: "Directed evolution of a maltogenic alpha-amylase from Bacillus sp. TS-25", J. BIOTECHNOL., vol. 134, 2008, pages 325 - 333
BESSLER, C. ET AL.: "Directed evolution of a bacterial alpha-amylase: toward enhanced pH-performance and higher specific activity", PROTEIN SCI., vol. 12, 2003, pages 2141 - 2149
LIU, Y. H. ET AL.: "Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR", J. APPL. MICROBIOL., vol. 113, July 2012 (2012-07-01), pages 541 - 549
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
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