AZAINDOLYLPYRIDONE AND DIAZAINDOLYLPYRIDONE COMPOUNDS

FIELD OF THE INVENTION

The invention provides novel azaindolylpyridone and diazaindolylpyridone compounds of formula (I), pharmaceutical compositions containing such compounds, and methods for using such compounds in treatment of diseases including cancer and type II diabetes.

BACKGROUND OF THE INVENTION

Enzymes belonging to the family of phosphatidylinositide 3-kinases (PI3K) are regulators of several important cellular events. The family consists of three classes, I, II and III and while the Class I group has been an interesting drug target for many years, Class II and III are less exploited. The PI3K Class III, vacuolar protein sorting 34 (Vps34, PIK3C3) forms a heterodimer with its regulatory subunit p150 (Vps15) and this dimer takes part in several complexes regulating vesicular trafficking events such as autophagy, endocytosis, exocytosis and micropinocytosis (Amaravadi et al. Clin Cancer Res. 201 1 , 17:654-666; Carpentier et al. 2013, Traffic). The enzyme is responsible for phosphorylation of phosphatidyl inositol (PI) to

phosphatidylinositol (3)-phosphate (PI3P). The ligand binding to PX and FYVE domains results in recruiting and derealization of these effector proteins that lead to vesicular formation, elongation and movement (Backer et al. J Biochem. 2008, 410:1 -17).

Autophagy is a catabolic process where cellular components are targeted for degradation by enclosing them in double-membrane vesicles, auto- phagosomes that are fused with the protease-containing lysosomes. This is a mean for the cell to handle damaged organelles and misfolded proteins and by that maintain cellular function. The pathway is also a way of recirculating cellular content into new building blocks (Boya et al, Nat Cell Biol 2013, 15;713-720). Autophagy is a cellular response to stressful conditions as nutrient deprivation, acidosis and hypoxia but also to drug treatment. Therefore, autophagy inhibition is a means to potentiate cancer drugs and resensitize drug resistant tumors (Nagelkerke et al, Semin Cancer Biol 2014, 31 ; 99-105). Most advanced tumors show a high upregulation of autophagic flux (Leone et al. Trends in Endocrin Metab 2013, 24; 209-217). An established marker for studying autophagic flux is the detection of autophagic puncta in the form of lipidated LC3 protein on the autophagosome. Inhibition of Vps34 results in the inhibition of autophagy as measured by LC3 redistribution into puncta (Dowdle et al., Nat Cell Biol 2014, 16; 1069-79). As recently described, ablation of the regulatory subunit p150 leads to increased insulin sensitivity in vivo due to decreased insulin receptor internalization (Nemazanyy, Nature Commun., 2015, 6:8283). A kinase dead heterozygous animal model confirms this result with increased glucose tolerance and increased insulin sensitivity (WO2013076501 ).

Several disease states could benefit from Vps34 inhibition including cancer, inflammatory diseases, autoimmune diseases, neurodegenerative disorders, cardiovascular disorders, type II diabetes and viral infections (Reviewed in Rubinsztein et al, Nat Rev 2012, 1 1 ;709-730). Cancer forms that would benefit from Vps34 inhibition include, but are not limited to, breast cancer, such as triple negative breast cancer, bladder cancer, liver cancer, cervical cancer, pancreatic cancer, leukemia, lymphoma, renal cancer, colon cancer, glioma, prostate cancer, ovarian cancer, melanoma, and lung cancer as well as hypoxic tumors. There is thus a need for novel and potent inhibitors of Vps34.

Previous disclosures describing Vps34 inhibitors in use to affect diseases include WO2015150555; WO2015150557; WO2015108861 ;

WO2015108881 ; WO2012085815; WO2012085244; WO2013190510;

Farkas, J. Biol. Chem., 201 1 286(45) 38904-12. DESCRIPTION OF THE INVENTION

An object of the invention is to provide novel and potent inhibitors of Vps34. Another object of the invention is to provide novel and potent inhibitors of Vps34 that may be used for treating cancer and other diseases such as type II diabetes.

According to one aspect of the invention, there is provided a compound of Formula (I)

wherein

X is N or CR ¹ ;

R ¹ is selected from H, Ci-C ₃ alkyl, Ci-C ₃ haloalkyl, Ci-C ₃ alkoxyCi-C ₃ alkyl, C ₃ - C6cycloalkyl, cyano, phenyl, monocyclic heteroaryl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one or more substituents selected from halo, N-Ci-C ₃ alkylamino, N,N-diCi-C ₃ alkylamino, C ₃ -C6cycloalkyl , Ci-C ₃ alkoxyCi-C ₃ alkyl, Ci-C ₃ haloalkyl, Ci-C ₃ haloalkoxy, Ci- C ₃ alkoxy and Ci-C ₃ alkyl;

R ² is selected from hydrogen, Ci-C ₃ haloalkyl and Ci-C ₃ alkyl;

R ³ is selected from A, phenyl and monocyclic heteroaryl, said phenyl and said heteroaryl being optionally and independently substituted with one or more

R ⁴ ;

R ⁴ is independently selected from COR ⁵ , halogen, Ci-C6alkyl, Ci-C6alkoxy, Ci-C6haloalkoxy, amino, N-Ci-C ₃ alkylamino, N,N-diCi-C ₃ alkylamino, 1 - pyrrolidinyl, 1 -piperidinyl, 1 -azetidinyl, NHSO2R ⁶ , SO2R ⁷ , hydroxy, C ₃ - C6cycloalkyl, Ci-C ₃ alkoxyCi-C ₃ alkyl, Ci-C ₃ cyanoalkyl and Ci-C6haloalkyl; R ⁵ is selected from Ci-C ₃ alkoxy, N-Ci-C ₃ alkylamino, N,N-diCi-C ₃ alkylamino, 1 -pyrrol id inyl, 1 -piperidinyl and 1 -azetidinyl;

R ⁶ is Ci-C ₃ haloalkyl or Ci-C ₃ alkyl; R ⁷ is selected from R ⁸ , Ci-C6alkyl, N-Ci-C3alkylamino, N,N-diCi-C3alkylamino and Ci-C3alkoxyCi-C3alkyl, said Ci-C6alkyl and said Ci-C3alkoxyCi-C3alkyl being optionally substituted with one R ⁸ and/or one or more halo;

R ⁸ is selected from phenyl, monocyclic heteroaryl, C3-C6cycloalkyl,

heterocyclyl, each optionally substituted with one or more R ⁹ ;

R ⁹ is selected from halo, N-Ci-C3alkylamino, N,N-diCi-C3alkylamino, Ci- C3alkoxyCi-C3alkyl, amino, Ci-C3haloalkyl, Ci-C3alkoxy, Ci-C3haloalkoxy, C3- C6cycloalkyl and Ci-C3alkyl;

A is

R ¹⁰ is selected from hydrogen, halogen, COR ¹¹ , Ci-C6alkyl, Ci-C3alkoxyCi- Csalkyl, Ci-C6alkoxy, C3-C6cycloalkyl, Ci-C3cyanoalkyl, Ci-C3haloalkyl, phenyl and heteroaryl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one or more R ¹² , and provided that when R ¹⁰ is phenyl or heteroaryl, then X is N or CH;

R ¹¹ is selected from Ci-C3alkoxy, N-Ci-C3alkylamino, N,N-diCi-C3alkylamino, 1 -pyrrol id inyl, 1 -piperidinyl and 1 -azetidinyl;

Y is CH ₂ , S, SO, SO ₂ , NR ¹³ , NCOR ⁷ , NCOOR ¹⁴ , NSO2R ⁷ , NCOCH2R ⁷ , O, or a bond;

R ¹² is selected from Ci-C ₆ alkyl, Cs-Cecycloalkyl, Ci-C ₃ alkoxyCi-C ₃ alkyl, Ci- Cshaloalkyl, halogen, N-Ci-C3alkylamino, N,N-diCi-C3alkylamino, Ci- Cshaloalkoxy and Ci-C3alkoxy;

R ¹³ is selected from H, Ci-C ₃ haloalkyl, Ci-C ₃ alkoxyCi-C ₃ alkyl, Ci-C ₃ alkyl, C ₃ - C6cycloalkyl;

R ¹⁴ is selected from R ⁸ , Ci-C ₆ alkyl and Ci-C ₃ alkoxyCi-C ₃ alkyl, said Ci-C ₆ alkyl and said Ci-C3alkoxyCi-C3alkyl being optionally substituted with one R ⁸ and/or one or more halo;

or a pharmaceutically acceptable salt, or pharmaceutically acceptable salts thereof. According to one embodiment of this aspect of the invention, R ² is hydrogen or Ci-C3alkyl .

According to one embodiment of this aspect of the invention, R ¹ is selected from H, Ci-C3alkyl, Ci-C3haloalkyl, C3-C6cycloalkyl, cyano, phenyl, heteroaryl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one or more substituents selected from Ci-C3haloalkyl, halo, C3-C6cycloalkyl and Ci-C3alkyl .

According to one embodiment of this aspect of the invention, R ² is hydrogen.

According to one embodiment of this aspect of the invention, said heteroaryl in R ¹ is selected from pyridyl, oxazolyl, thienyl, and pyrimidinyl, each optionally and independently substituted with one or more substituents selected from halo, cyclopropyl, Ci-C3fluoroalkyl and Ci-C3alkyl .

According to one embodiment of this aspect of the invention, R ¹ is selected from

According to one embodiment of this aspect of the invention, R ¹ is selected from

According to one embodiment of this aspect of the invention, R ³ is selected from A, phenyl and monocyclic heteroaryl selected from pyridyl, thienyl, furyl, pyrimidinyl and pyrazolyl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one or two R ⁴ .

According to one embodiment of this aspect of the invention, R ³ is selected from A, phenyl and monocyclic heteroaryl selected from pyridyl, thienyl and pyrazolyl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one or two R ⁴ . According to one embodiment of this aspect of the invention, R ³ is selected from A, phenyl and pyridyl, wherein said phenyl and said pyridyl are optionally substituted with one R ⁴ .

According to one embodiment of this aspect of the invention, R ³ is selected from A, phenyl and pyridyl, wherein said phenyl and said pyridyl are optionally and independently substituted with one or two R ⁴ .

According to one embodiment of this aspect of the invention, R ⁴ is selected from fluoro, chloro, Ci-C3alkyl, C3-C6cycloalkyl, Ci-C3fluoroalkyl and SO2R ⁷ .

According to one embodiment of this aspect of the invention, R ⁴ is selected from chloro, Ci-C ₃ alkyl, Ci-C ₃ fluoroalkyl and SO2R ⁷ . According to one embodiment of this aspect of the invention, Y is CH2, NSO2R ⁷ , O or a bond. According to one embodiment of this aspect of the invention, R ¹⁰ is selected from hydrogen, Ci-C3alkyl, C3-C6cycloalkyl, phenyl, monocyclic heteroaryl and Ci-C3haloalkyl, wherein said phenyl and said heteroaryl are optionally and independently substituted with one R ¹² ; and

R ¹² is selected from Ci-C3alkyl, cyclopropyl, CF3, halogen, Ci-C3haloalkoxy and Ci-C3alkoxy.

According to one embodiment of this aspect of the invention, R ¹⁰ is selected from hydrogen, Ci-C3alkyl, phenyl, Ci-C3haloalkyl.

According to one embodiment of this aspect of the invention, R ⁷ is selected from R ⁸ , N,N-diCi-C3alkylamino, Ci-C3alkyl and methoxyCi-Csalkyl, said Ci- Csalkyl being optionally substituted with one R ⁸ .

According to one embodiment of this aspect of the invention, R ⁷ is selected from Ci-C3alkyl and fluorophenyl.

According to one embodiment of this aspect of the invention, R ⁷ is selected from Ci-C3alkyl and fluorobenzyl.

According to one embodiment of this aspect of the invention, R ⁸ is selected from phenyl, pyridyl, imidazolyl, isoxazolyl, oxazolyl, cyclopropyl, cyclopentyl, pyrrolidinyl, tetrahydrofuryl, each optionally substituted with one or more substituents selected from cyclopropyl, methyl and fluoro. According to one embodiment of this aspect of the invention, R ³ is selected from

According to one embodiment of this aspect of the invention, R ³ is selected from

According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from Ch , O and a bond;

R ⁴ is selected from CF3, fluoro and chloro, cyclopropyl and methyl; and R ¹⁰ is selected from cyclopropyl, methyl, fluorophenyl, chlorophenyl, methoxyphenyl and CF3.

According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from CH2, O and a bond;

R ⁴ is selected from CF3, chloro, and methyl; and

R ¹⁰ is selected from methyl, phenyl and CF3. According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from Ch , O, NSO2R ⁷ and a bond;

R ⁴ is selected from CF3, fluoro, cyclopropyl and methyl; and

R ¹⁰ is selected from hydrogen, phenyl, cyclopropyl, methyl, and CF3.

According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from CH2, O, NSO2R ⁷ and a bond;

R ⁴ is selected from CF3, chloro and methyl; and

R ¹⁰ is selected from hydrogen, phenyl, methyl, and CF3.

According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from CH2, O and a bond;

R ⁴ is selected from CF3, cyclopropyl, fluoro and chloro; and

R ¹⁰ is CF3 or cyclopropyl.

According to one embodiment of this aspect of the invention, R ³ is selected from

wherein Y is selected from Ch , O and a bond;

R ⁴ is selected from CF3 and chloro; and

R ¹⁰ is CF ₃ .

According to one embodiment of this aspect of the invention, X is N.

According to one embodiment of this aspect of the invention, X is CR ¹ .

According to one embodiment of this aspect of the invention, R ¹ is selected from

R ² is hydrogen; and

R ³ is selected from

According to one embodiment of this aspect of the invention, R ¹ is selected from

R ² is hydrogen; and

R ³ is selected from

R ² is hydrogen; and R ³ is selected from

According to one embodiment of this aspect of the invention, X is N; R ² is hydrogen; and

R ³ is selected from

According to one embodiment of this aspect of the invention, said compound is

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)phenyl]- 1 H-pyridin-2- one;

6-(3-Methyl-4-pyridyl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one;

6-(2-phenylpyrrolidin-1 -yl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one;

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-(3-pyridyl)-1 H-pyridin-2-one;

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-morpholino-1 H-pyridin-2-one;

6-(2-Chlorophenyl)-4-(2-oxazol-5-yl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin- 2-one;

6-(2-Chlorophenyl)-4-[2-(3-pyridyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2- one; 6-(2-Chlorophenyl)-4-(2-methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyndin-2- one;

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -pipendyl]- 1 H-pyridin-2-one;

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoronnethyl)-1 -piperidyl]-1 H-pyridin- 2-one;

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[3-(trifluoromethyl)morpholi n-4-yl]-1 H^ pyridin-2-one;

6-[3-(Trifluoromethyl)morpholin-4-yl]-4-[2-[3-(trifluorometh yl)phenyl]-1 H pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

4-[2-(5-Methyl-2-thienyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-6-[3- (trifluoromethyl)morpholin-4-yl]-1 H-pyridin-2-one;

4-(1 H-pyrazolo[3,4-b]pyridin-4-yl)-6-[2-(trifluoronnethyl)-1 -piperidyl]-1 H-pyridin- 2-one;

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[2-(trifluoronnethyl)pyrinnidin-5-yl]-1 H- pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[6-(tnfluoromethyl)-3-pyndyl]-1 ^ pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[5-(tnfluoromethyl)-3-pyndyl]^ pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

4-(2-cyclopropyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[4-ethylsulfonyl-2- (trifluoronnethyl)piperazin-l -yl]-1 H-pyridin-2-one;

6-[4-ethylsulfonyl-2-(trifluoronnethyl)piperazin-1 -yl]-4-(1 H-pyrrolo[2,3-b]pyridin- 4-yl)-1 H-pyridin-2-one;

6-[4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluoromethyl) piperazin-1 -yl]-4-(1 H- pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one;

6-[4-Ethylsulfonyl-2-(trifluoromethyl)piperazin-1 -yl]-4-(2-methyl-1 H- pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one;

4-[2-[4-Ethylsulfonyl-2-(trifluoronnethyl)piperazin-1 -yl]-6-oxo-1 H-pyridin-4-yl]- 1 H-pyrrolo[2,3-b]pyridine-2-carbonitrile;

4-(2-cyclopropyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoronnethyl)phenyl] - 1 H-pyridin-2-one; 4-[2-Oxo-6-[2-(trifluoromethyl)phenyl]-1 H-pyndin-4-yl]-1 H-pyrrolo[2,3- b]pyridine-2-carbonitrile;

4-(1 H-pyrazolo[3,4-b]pyridin-4-yl)-6-[2-(trifluoromethyl)phenyl] -1 H-pyndin one;

6-[4-Methylsulfonyl-2-(thfluoronnethyl)piperazin-1 -yl]-4-(1 H-pyrazolo[3,4- b]pyridin-4-yl)-1 H-pyhdin-2-one;

According to one embodiment of this aspect of the invention, said compound is

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)phenyl]- 1 H-pyridin-2- one;

6-(3-Methyl-4-pyridyl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one;

6-(2-phenylpyrrolidin-1 -yl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one; 4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-(3-pyridyl)-1 H-pyridin-2-one;

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-morpholino-1 H-pyridin-2-one; 6-(2-Chlorophenyl)-4-(2-oxazol-5-yl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin- 2-one;

6-(2-Chlorophenyl)-4-[2-(3-pyridyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2- one;

6-(2-Chlorophenyl)-4-(2-methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2- one;

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -piperidyl]- 1 H-pyridin-2-one;

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -piperidyl]-1 H-pyridin- 2-one;

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[3-(trifluoromethyl)morpholi n-4-yl]-1 H- pyridin-2-one;

6-[3-(Trifluoromethyl)morpholin-4-yl]-4-[2-[3-(trifluorometh yl)phenyl]-1 H- pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

4-[2-(5-Methyl-2-thienyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-6-[3- (trifluoromethyl)morpholin-4-yl]-1 H-pyridin-2-one;

4-(1 H-pyrazolo[3,4-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -piperidyl]-1 H-pyridin- 2-one; 6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[2-(tnfluoromethyl)pynmi^

pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[6-(tnfluoromethyl)-3-pyn^

pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[5-(trifluoromethyl)-3-pyridyl]-1 H- pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one;

4-(2-cyclopropyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[4-ethylsulfonyl-2- (trifluoronnethyl)piperazin-l -yl]-1 H-pyridin-2-one;

6-[4-ethylsulfonyl-2-(trifluoronnethyl)piperazin-1 -yl]-4-(1 H-pyrrolo[2,3-b]pyridin- 4-yl)-1 H-pyridin-2-one; or

6-[4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluoro H- pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one.

In one aspect of the invention, there is provided a compound according to the present invention, for use in the treatment or prophylaxis of a disease. In one aspect of the invention, there is provided a compound according to the present invention, for use in treating cancer. Typically, said cancer is selected from breast cancer, such as triple negative breast cancer, bladder cancer, liver cancer, cervical cancer, pancreatic cancer, leukemia, lymphoma, renal cancer, colon cancer, glioma, prostate cancer, ovarian cancer, melanoma and lung cancer, as well as hypoxic tumors.

In one aspect of the invention, there is provided a compound according to the present invention, for use in treating hypoxic tumors.

In one aspect of the invention, there is provided a compound according to the present invention, for use in treating type II diabetes. In one aspect of the invention, there is provided a compound according to the present invention, for use in treating a disease selected from inflammatory diseases, autoimmune diseases, neurodegenerative disorders,

cardiovascular disorders and viral infections. In one aspect of the invention, there is provided use of a compound according to the present invention, in the preparation of a medicament for treating cancer. Typically said cancer is selected from breast cancer, such as triple negative breast cancer, bladder cancer, liver cancer, cervical cancer, pancreatic cancer, leukemia, lymphoma, renal cancer, colon cancer, glioma, prostate cancer, ovarian cancer, melanoma and lung cancer, as well as hypoxic tumors.

In one aspect of the invention, there is provided use of a compound according to the present invention, in the preparation of a medicament for treating hypoxic tumors.

In one aspect of the invention, there is provided use of a compound according to the present invention, in the preparation of a medicament for treating type II diabetes.

In one aspect of the invention, there is provided use of a compound according to the present invention, in the preparation of a medicament for treating a disease selected from inflammatory diseases, autoimmune diseases, neurodegenerative disorders, cardiovascular disorders and viral infections.

In one aspect of the invention, there is provided a method of treating cancer, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof. Typically, said cancer is selected from breast cancer, such as triple negative breast cancer, bladder cancer, liver cancer, cervical cancer, pancreatic cancer, leukemia, lymphoma, renal cancer, colon cancer, glioma, prostate cancer, ovarian cancer, melanoma and lung cancer, as well as hypoxic tumors. In one aspect of the invention, there is provided a method of treating hypoxic tumors, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof. In one aspect of the invention, there is provided a compound according to the present invention, for use in treating cancer, wherein said cancer treatment further comprises radiation therapy.

In one aspect of the invention, there is provided a method of treating cancer, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof, in conjunction with radiation therapy.

The compounds of the present invention may also be employed in cancer treatment in conjunction with radiation therapy and/or surgical intervention. Generally, the use of cytotoxic and/or cytostatic agents in combination with a compound or composition of the present invention will serve to:

(1 ) yield better efficacy in reducing the growth of a tumor or even eliminate the tumor as compared to administration of either agent alone,

(2) provide for the administration of lesser amounts of the administered chemotherapeutic agents,

(3) provide for a chemotherapeutic treatment that is well tolerated in the patient with fewer deleterious pharmacological complications than observed with single agent chemotherapies and certain other combined therapies,

(4) provide for treating a broader spectrum of different cancer types in mammals, especially humans,

(5) provide for a higher response rate among treated patients,

(6) provide for a longer survival time among treated patients compared to standard chemotherapy treatments,

(7) provide a longer time for tumor progression, and/or

(8) yield efficacy and tolerability results at least as good as those of the agents used alone, compared to known instances where other cancer agent combinations produce antagonistic effects.

In one aspect of the invention, there is provided a method of treating hypoxic tumors, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof. In one aspect of the invention, there is provided a method of treating type II diabetes, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof.

In one aspect of the invention, there is provided a method of treating a disease selected from inflammatory diseases, autoimmune diseases, neurodegenerative disorders, and viral infections, comprising administering a therapeutically effective amount of a compound according to the present invention, to a patient in need thereof.

In one aspect of the invention, there is provided a pharmaceutical

composition comprising a compound according to the present invention, and a pharmaceutically acceptable diluent, carrier and/or excipient.

In one aspect of the invention, there is provided a pharmaceutical

composition, comprising a therapeutically effective amount of a compound according to the present invention and another anticancer agent selected from alkylating agents, antimetabolites, anticancer camptothecin derivatives, plant-derived anticancer agents, antibiotics, enzymes, platinum coordination complexes, tyrosine kinase inhibitors, hormones, hormone antagonists, monoclonal antibodies, interferons, and biological response modifiers.

As used herein, the term "Ci-C6alkyl" means both linear and branched chain saturated hydrocarbon groups with 1 to 6 carbon atoms. Examples of Ci- Cealkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, t-butyl, n-pentyl, 4-methyl-butyl, n-hexyl, 2-ethyl-butyl groups.

Among unbranched Ci-C6alkyl groups, typical ones are methyl, ethyl, n- propyl, n-butyl, n-pentyl and n-hexyl groups. Among branched alkyl groups, there may be mentioned iso-propyl, iso-butyl, sec-butyl, t-butyl, 4-methyl-butyl and 2-ethyl-butyl groups.

As used herein, the term "Ci-C3alkyl" means both linear and branched chain saturated hydrocarbon groups with 1 to 3 carbon atoms. Examples of Ci- Csalkyl groups include methyl, ethyl, n-propyl and isopropyl groups. As used herein, the term "Ci-C6alkoxy" means the group O-alkyl, where "Ci- Cealkyl" is used as described above. Examples of Ci-C6alkoxy groups include, but are not limited to, methoxy, ethoxy, isopropoxy, n-propoxy, n- butoxy, n-hexoxy, 3-methyl-butoxy groups. As used herein, the term "Ci-C3alkoxy" means the group O-alkyl, where "Ci- Csalkyl" is used as described above. Examples of Ci-C3alkoxy groups include, but are not limited to, methoxy, ethoxy, isopropoxy and n-propoxy.

As used herein, the term "Ci-C6haloalkyl" means both linear and branched chain saturated hydrocarbon groups, with 1 to 6 carbon atoms and with 1 to all hydrogens substituted by a halogen of different or same type. Examples of Ci-C6haloalkyl groups include methyl substituted with 1 to 3 halogen atoms, ethyl substituted with 1 to 5 halogen atoms, n-propyl or iso-propyl substituted with 1 to 7 halogen atoms, n-butyl or iso-butyl substituted with 1 to 9 halogen atoms, and sec-butyl or t-butyl groups substituted with 1 to 9 halogen atoms. As used herein, the term "Ci-C3haloalkyl" means both linear and branched chain saturated hydrocarbon groups, with 1 to 3 carbon atoms and with 1 to all hydrogens substituted by a halogen of different or same type. Examples of Ci-C3haloalkyl groups include methyl substituted with 1 to 3 halogen atoms, ethyl substituted with 1 to 5 halogen atoms, and n-propyl or iso-propyl substituted with 1 to 7 halogen atoms.

As used herein, the term "Ci-C3haloalkoxy" means both linear and branched chain saturated alkoxy groups, with 1 to 3 carbon atoms and with 1 to all hydrogen atoms substituted by a halogen atom of different or same type. Examples of Ci-C3haloalkoxy groups include methoxy substituted with 1 to 3 halogen atoms, ethoxy substituted with 1 to 5 halogen atoms, and n-propoxy or iso-propoxy substituted with 1 to 7 halogen atoms.

As used herein, the term "Ci-C3fluorooalkyl" means both linear and branched chain saturated hydrocarbon groups, with 1 to 3 carbon atoms and with 1 to all hydrogen atoms substituted by a fluorine atom. Examples of Ci- Csfluoroalkyl groups include methyl substituted with 1 to 3 fluorine atoms, ethyl substituted with 1 to 5 fluorine atoms, and n-propyl or iso-propyl substituted with 1 to 7 fluorine atoms.

As used herein, the term "Ci-C3fluorooalkoxy" means both linear and branched chain saturated alkoxy groups, with 1 to 3 carbon atoms and with 1 to all hydrogen atoms substituted by a fluorine atom. Examples of Ci-

Csfluoroalkoxy groups include methoxy substituted with 1 to 3 fluorine atoms, ethoxy substituted with 1 to 5 fluorine atoms, and n-propoxy or iso-propoxy substituted with 1 to 7 fluorine atoms.

As used herein, the term "C3-C6cycloalkyl" means a cyclic saturated hydrocarbon group, with 3 to 6 carbon atoms. Examples of C3-C6cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

As used herein, the term "Ci-C3alkoxyCi-C3alkyl" means both a linear and branched chain saturated hydrocarbon group, with 1 to 3 carbon atoms, substituted with an alkoxy group with 1 to 3 carbon atoms. Examples of Ci- C3alkoxyCi-C3alkyl grou s are drawn belo

As used herein, the term "Ci-C3cyanoalkyl" means both a linear and branched chain cyano (CN) derivative, with one to three carbon atoms including the carbon atom that is part of the cyano group. Examples of Ci- Cscyanoalkyl groups are drawn below.

As used herein, the term N-Ci-C3alkylamino means an amino substituent carrying one Ci-C3alkyl group as defined supra. Examples of N-Ci- C3alkylamino are drawn below.

H

As used herein, the term N,N-diCi-C3alkylamino means an amino substituent carrying two Ci-C3alkyl groups as defined supra. Examples of N,N-diCi- C3alkylamino are drawn below. As used herein, the term "halogen" means fluorine, chlorine, bromine or iodine. As used herein, the term "halo" means fluoro, chloro, bromo or iodo.

As used herein, the term "aryl" means a monocyclic aromatic carbocyclic group. An examples of such group include phenyl.

As used herein, the term "monocyclic aryl" means a monocyclic aromatic carbocyclic group. Examples of monocyclic aryl groups include phenyl.

As used herein, the term "heteroaryl" means a monocyclic or bicyclic aromatic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur. In a bicyclic aryl, one of the rings may be partially saturated. Examples of such groups include indolinyl, dihydrobenzofuran and 1 ,3- benzodioxolyl.

As used herein, the term "monocyclic heteroaryl" means a monocyclic aromatic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen or sulfur.

Examples of monocyclic heteroaryl groups include, but are not limited to, furyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, oxadiazolyl, thiadiazolyl, pyridyl, triazolyl, triazinyl, pyridazyl, isothiazolyl, isoxazolyl, pyrazinyl, pyrazolyl, and pyrimidinyl. Examples of bicyclic heteroaryl groups include, but are not limited to, quinoxalinyl, quinazolinyl, pyridopyrazinyl, benzoxazolyl, benzothiophenyl, benzimidazolyl, naphthyridinyl, quinolinyl, benzofuryl, indolyl, indazolyl, benzothiazolyl, pyndopyrimidinyl, and isoquinolinyl.

As used herein, the term "heterocyclyl" means a cyclic group of carbon atoms wherein from one to three of the carbon atoms is/are replaced by one or more heteroatoms independently selected from nitrogen, oxygen and sulfur.

Examples of heterocyclyl groups include, but are not limited to,

tetrahydrofuryl, tetrahydropyranyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl and dioxanyl.

Depending on the substituents present in compounds of the formula (I), the compounds may form salts which are within the scope of the present invention. Salts of compounds of formula (I), which are suitable for use in medicine are those wherein a counterion is pharmaceutically acceptable.

Suitable salts according to the invention include those formed with organic or inorganic acids or bases. In particular, suitable salts formed with acids according to the invention include those formed with mineral acids, strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted, for example, by halogen, such as saturated or unsaturated dicarboxylic acids, such as hydroxycarboxylic acids, such as amino acids, or with organic sulfonic acids, such as (Ci- C ₄ )alkyl or aryl sulfonic acids which are unsubstituted or substituted, for example by halogen. Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulphuric, nitric, citric, tartaric, acetic, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, succinic, perchloric, fumaric, maleic, glycolic, lactic, salicylic, oxaloacetic, methanesulfonic, ethanesulfonic, p-toluenesulfonic, formic, benzoic, malonic, naphthalene-2- sulfonic, benzenesulfonic, isethionic, ascorbic, malic, phthalic, aspartic, and glutamic acids, lysine and arginine.

Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts, for example those of potassium and sodium, alkaline earth metal salts, for example those of calcium and magnesium, and salts with organic bases, for example dicyclohexylamine, N-methyl-D-glucamine, morpholine, thiomorpholine, piperidine, pyrrolidine, a mono, di- or tri lower alkylamine, for example ethyl, tertbutyl, diethyl, diisopropyl, triethyl, tributyl or

dimethylpropylamine, or a mono- ,di- or trihydroxy lower alkylamine, for example mono-, di- or triethanolamine. Corresponding internal salts may furthermore be formed.

The compounds of the invention may be used in the prophylaxis and/or treatment as such, or in a form of a pharmaceutical composition. While it is possible for the active ingredient to be administered alone, it is also possible for it to be present in a pharmaceutical composition. Accordingly, the invention provides a pharmaceutical composition comprising a compound of formula (I), and a pharmaceutically acceptable diluent, excipient and/or carrier. Pharmaceutical compositions of the invention may take the form of a pharmaceutical composition as described below. Exemplary compositions for oral administration include suspensions which can contain, for example, microcrystalline cellulose for imparting bulk, alginic acid or sodium alginate as a suspending agent, methylcellulose as a viscosity enhancer, and sweeteners or flavoring agents such as those known in the art; and immediate release tablets which can contain, for example,

microcrystalline cellulose, dicalcium phosphate, starch, magnesium stearate, calcium sulfate, sorbitol, glucose and/or lactose and/or other excipients, binders, extenders, disintegrants, diluents and lubricants such as those known in the art. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like. Disintegrators include without limitation starch, methylcellulose, agar, bentonite, xanthan gum and the like. The compounds of formula (I) can also be delivered through the oral cavity by sublingual and/or buccal administration. Molded tablets, compressed tablets or freeze-dried tablets are exemplary forms which may be used. Exemplary compositions include those formulating the present compound(s) with fast dissolving diluents such as mannitol, lactose, sucrose and/or cyclodextrins. Also included in such compositions may be high molecular weight excipients such as celluloses (avicel) or polyethylene glycols (PEG). Such compositions can also include an excipient to aid mucosal adhesion such as hydroxy propyl cellulose (HPC), hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (SCMC), maleic anhydride copolymer (e.g., Gantrez), and agents to control release such as polyacrylic copolymer (e.g. Carbopol 934). Lubricants, glidants, flavors, coloring agents and stabilizers may also be added for ease of fabrication and use. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. For oral

administration in liquid form, the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Compositions of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, pills or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a nonaqueous liquid, for example as elixirs, tinctures, suspensions or syrups; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste.

A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein. The present compounds can, for example, be administered in a form suitable for immediate release or extended release. Immediate release or extended release can be achieved by the use of suitable pharmaceutical compositions comprising the present compounds, or, particularly in the case of extended release, by the use of devices such as subcutaneous implants or osmotic pumps. The present compounds can also be administered liposomally.

Typical unit dosage compositions are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.

It should be understood that in addition to the ingredients particularly mentioned above, the compositions of this invention may include other agents conventional in the art having regard to the type of composition in question, for example those suitable for oral administration may include flavoring agents.

The compositions may be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. Methods may include the step of bringing the active ingredient into association with the carrier which constitutes one or more accessory ingredients. Compositions may be prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired composition.

The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, 1 ,2-dipalmitoylphosphatidylcholine, phosphatidyl ethanolamine (cephaline), phosphatidylserine,

phosphatidylinositol, diphosphatidylglycerol (cardiolipin) or

phosphatidylcholine (lecithin).

Compositions for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers,

bacteriostats and solutes which render the composition isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The

compositions may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use.

Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.

Exemplary compositions for parenteral administration include injectable solutions or suspensions which can contain, for example, suitable non-toxic, parenterally acceptable diluents or solvents, such as polyethylene glycol, ethanol, 1 ,3-butanediol, water, Ringer's solution, an isotonic sodium chloride solution, or other suitable dispersing or wetting and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid, or Cremaphor.

Exemplary compositions for nasal, aerosol or inhalation administration include solutions in saline, which can contain, for example, benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, and/or other solubilizing or dispersing agents such as those known in the art. Compositions for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, synthetic glyceride esters or polyethylene glycol. Such carriers are typically solid at ordinary temperatures, but liquefy and/or dissolve in the rectal cavity to release the drug.

Compositions for topical administration in the mouth, for example buccally or sublingually, include lozenges comprising the active ingredient in a flavored basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerine or sucrose and acacia. Exemplary compositions for topical administration include a topical carrier such as Plastibase (mineral oil gelled with polyethylene). Compounds of formula (I) may be administered as the sole pharmaceutical agent or in combination with one or more additional therapeutic agents where the combination causes no unacceptable adverse effects. This

pharmaceutical composition includes administration of a single

pharmaceutical dosage composition which contains a compound of formula (I) and one or more additional therapeutic agents, as well as administration of the compound of formula (I) and each additional therapeutic agent in its own separate pharmaceutical dosage composition. For example, a compound of formula (I) and a therapeutic agent may be administered to the patient together in a single oral dosage composition such as a capsule or tablet, or each agent may be administered in compositions with separate dosage.

Where separate dosage compositions are used, the compound of formula (I) and one or more additional therapeutic agents may be administered at essentially the same time (e.g., concurrently) or at separately staggered times (e.g., sequentially).

The amount of active ingredient which is required to achieve a therapeutic effect will, of course, vary with the particular compound, the route of administration, the subject under treatment, including the type, species, age, weight, sex, and medical condition of the subject and the renal and hepatic function of the subject, and the particular disorder or disease being treated, as well as its severity. An ordinarily skilled physician, veterinarian or clinician can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.

Oral dosages of the present invention, when used for the indicated effects, will range between about 0.01 mg per kg of body weight per day (mg/kg/day) to about 100 mg/kg/day, preferably 0.01 mg per kg of body weight per day (mg/kg/day) to 10 mg/kg/day, and most preferably 0.1 to 5.0 mg/kg/day, for adult humans. For oral administration, the compositions may be provided in the form of tablets or other forms of presentation provided in discrete units containing 0.01 , 0.05, 0.1 , 0.5, 1 .0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. A medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from about 1 mg to about 100 mg of active ingredient. Intravenously, the most preferred doses will range from about 0.1 to about 10 mg/kg/minute during a constant rate infusion. Compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in the art. To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen. Preparation of compounds

The compounds in the present invention can be prepared as a free base or a pharmaceutically acceptable salt thereof by the process described below. Throughout the following description of such processes it is understood that, where appropriate, suitable protecting groups will be added to, and

subsequently removed from the various reactants and intermediates in a manner that will be readily understood by one skilled in the art of organic synthesis. Conventional procedures for using such protecting groups as well as examples of suitable protecting groups are for example described in Protective Groups in Organic Synthesis by T.W. Greene, P.G.M Wutz, 4 ^th Edition, Wiley-lnterscience, New York, 2006. It is understood that microwaves can alternatively be used for the heating of reaction mixtures.

Another aspect of the present invention provides a process for preparing a compound of formula (I), or a pharmaceutically acceptable salt thereof, wherein R ² , R ³ and X are, unless specified otherwise, as defined herein. Said process comprises of: (i) formation of a corresponding compound of formula (I)

Scheme 1

A compound of formula (I) may be obtained (Scheme 1 ) by starting from, for example, a compound of formula (II), wherein R ^x may be F, OCH3, OC(CH3)3, or OSiR'R"R"' (wherein R', R" and R'" are independently aryl (such as phenyl) or alkyl (such as methyl or terf-butyl)). If R ^x is F the conversion into (I) may be carried out by for instance acidic hydrolysis using aqueous HCI. If R ^x is OCH3 the conversion into (I) may be carried out by reaction with for instance trimethylsilyl iodide in a suitable solvent such as chloroform or by reaction with HBr in a suitable solvent such as acetic acid or by reaction with BBr3 in a suitable solvent such as dichloromethane. If R ^x is OC(CH3)3 the conversion into (I) may be carried out by reaction with for instance

trifluoroacetic acid in a suitable solvent such as dichloromethane. If R ^x is OSiR'R"R"' the conversion into (I) may be carried out by for instance HCI in a suitable solvent such as methanol or by using tetrabutyl ammonium fluoride in tetrahydrofuran. If enantiomerically pure or enriched compound (II) is used in this reaction, an enantiomerically pure or enantiomerically enriched

compound (I) is obtained.

Compounds of formula (II) are commercially available compounds, or are known in the literature, or they are prepared by standard processes known in the art. A compound of formula (I) or (II) may be separated into its enantiomers by standard processes known in the art by for example chromatography on a chiral stationary phase.

General Methods

All solvents used were of analytical grade and commercially available anhydrous solvents were routinely used for reactions. Starting materials were available from commercial sources, or prepared according to literature procedures. Room temperature refers to +20-25 °C. Solvent mixture compositions are given as volume percentages or volume ratios.

Microwave heating was performed in a Biotage Initiator microwave cavity producing continuous irradiation at 2.45 GHz. It is understood that

microwaves may be used for the heating of reaction mixtures.

Straight phase chromatography was manually performed on Merck Silica gel 60 (0.040-0.063 mm), or automatically using an ISCO Combiflash®

Companion™ system using SiliaSep™ normal-phase flash columns using the solvent system indicated.

NMR spectra were recorded on a 400 MHz (or higher field) NMR

spectrometer fitted with a probe of suitable configuration. Spectra were recorded at ambient temperature unless otherwise stated. Chemical shifts are given in ppm down- and upfield from TMS (0.00 ppm). The following reference signals were used: the residual solvent signal of DMSO-c/6 δ 2.5, CDCI3 δ 7.26 or Methanol-c/4 δ 3.31 . Resonance multiplicities are denoted s, d, t, q, m and br for singlet, doublet, triplet, quartet, multiplet and broad, respectively.

High pressure liquid chromatography (HPLC) was performed on a reverse phase column. A linear gradient was applied using for example mobile phase A (aqueous 0.1 % NH3 or aqueous 0.1 % acetic acid or aqueous 0.1 % formic acid) and B (acetonitrile or methanol). Mass spectrometer (MS) analyses were performed in positive ion mode using electrospray ionization (ES+). Preparative chromatography was run on a Gilson-PREP GX271 or GX281 with Trilution Ic as software on a reverse phase column. A linear gradient was applied using for example mobile phase A (aqueous 0.1 % NH3 or aqueous 0.1 % acetic acid or aqueous 0.1 % formic acid) and B (acetonitrile or methanol).

Preparative chiral chromatography for separation of enantiomers was run on a Thar SFC using supercritical fluid chromatography on a chiral stationary phase. A linear gradient was applied using mobile phase A (carbon dioxide) and B (acetonitrile or methanol or ethanol or 2-propanol or any mixtures thereof). Additives (such as diethyl amine or isopropyl amine or ammonia or formic acid or TFA) may be used.

Compounds have been named using BIOVIA Draw 16.1 .

Abbreviations

Amphos (4-(N,N-Dimethylamino)phenyl)di-tert-butyl phosphine anh. anhydrous

aq. aqueous

BuLi butyl lithium

DCM dichloromethane

DMAc Λ/,/V-di methyl acetamide

DME 1 ,2-Dimethoxyethane

DMF Λ/,/V-di methyl formamide

DMSO dimethyl sulfoxide

EtOAc ethyl acetate

EtOH ethanol

h hour(s)

HPLC high pressure (or performance) liquid chromatography KOtBu potassium terf-butoxide

LCMS liquid chromatography mass spectrometry

MeCN acetonitrile

2-MeTHF 2-methyl tetrahydrofuran

MeOH methanol

min. minute(s)

NMR nuclear magnetic resonance PEPPSI-iPr [1 ,3-Bis(2,6-Diisopropylphenyl)imidazol-2-ylidene](3- chloropyridyl)palladiunn(ll) dichloride

Pd(OAc) ₂ palladium(ll) acetate

PdCl2(dppf) [1 ,1 '-Bis(diphenylphosphino)ferrocene]- dichloropalladium(ll)

quant. quantitative

rt room temperature

sat. Saturated

S-Phos 2-Dicyclohexylphosphino-2',6'-dimethoxybiphenyl TFA trifluoroacetic acid

THF tetrahydrofuran

Intermediate example 1

tert-Butyl 4-(2,6-dichloro-4-pyridyl)pyrrolo[2,3-b]pyridine-1 -carboxylate

tert-Butyl 4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)pyrrolo[2,3-b]pyridine- 1 -carboxylate (1 .07 g, 3.1 mmol), 2,6-dichloro-4-iodo-pyridine (850 mg, 3.1 mmol), PdCI ₂ (PPh ₃ )2 (109 mg, 0.16 mmol) and K ₂ CO ₃ (1 .07 g, 7.76 mmol) were taken up in DME:H2O:EtOH (6:3:1 , 8 ml) and the resulting mixture was stirred at 70 °C over night. When cooled to rt the mixture was concentrated and the resulting residue was taken up in DCM (10 ml). The mixture was filtered and the filtrate was purified on a silica gel column eluted with 0-40% EtOAc in heptane to give the product as a solid (270 mg, 33%). MS ES+ m/z 365 [M+H] ⁺ .

Intermediate example 2

4-(2-tert-Butoxy-6-chloro-4-pyridyl)-1 H-pyrrolo[2,3-b]pyridine

tert-Butyl 4-(2,6-dichloro-4-pyridyl)indole-1 -carboxylate (500 mg, 1 .37 mmol), KOtBu (462 mg, 4.12 mmol) and toluene (10 ml) were mixed and stirred at 100 °C for 5 h. When cooled to rt the mixture was diluted with EtOAc (20 ml) and 1 M aq. HCI (10 ml) was added. After 10 min the pH was adjusted to 7 using sat. aq. NaHCO3. The organic layer was separated and the aqueous layer was extracted with EtOAc (2 x 10 ml). The combined organics were washed with brine, dried over Na2SO ₄ , filtered and concentrated to give the product as a gum (460 mg, quant.). MS ES+ m/z 302 [M+H] ⁺ .

Intermediate example 3

4-[2-tert-Butoxy-6-[2-(trifluoromethyl)phenyl]-4-pyridyl] -1 H-pyrrolo[2,3- b]pyridine

4-(2-tert-Butoxy-6-chloro-4-pyridyl)-1 H-pyrrolo[2,3-b]pyridine (220 mg, 0.73 mmol), [2-(trifluoromethyl)phenyl]boronic acid (190 mg, 1 mmol), PdCl2(dppf) (30 mg, 0.04 mmol) and K2CO3 (302 mg, 2.19 mmol) were taken up in 1 ,4- dioxane:H2O:EtOH (6:3:1 , 4 ml) and the resulting mixture was stirred at 80 °C over night. When cooled to rt EtOAc (3 ml) and brine (3 ml) were added and the organic layer separated. The aqueous layer was extracted with EtOAc (2 x 3 ml). The combined organics were dried over Na2SO ₄ , filtered and concentrated to give the product (300 mg, quant.). MS ES+ m/z 412 [M+H] ⁺ .

Example 4

4-(1 H^yrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)phenyl]-1 H^yridin- 2-one

4-[2-tert-Butoxy-6-[2-(trifluoromethyl)phenyl]-4-pyridyl] -1 H-pyrrolo[2,3- b]pyridine (300 mg, 0.73 mmol) was taken up in DCM (3 ml) at rt. TFA (0.27 ml, 3.65 mmol) was added and the resulting mixture was stirred for 1 h. The mixture was concentrated and the resulting residue was taken up in EtOAc (10 ml) and sat. aq. NaHCO3. The organic layer was separated and the aqueous layer was extracted with EtOAc (2 x 10 ml). The combined organics were dried over Na2SO ₄ , filtered, concentrated and purified by preparative HPLC to give the product as a solid (14 mg, 5%). ¹ H NMR (500MHz, DMSO- cfe) δ = 12.10 (br. s., 1 H), 1 1 .95 (br. s., 1 H), 8.30 (d, 1 H), 7.89 (d, 1 H), 7.83 - 7.76 (m, 1 H), 7.75 - 7.65 (m, 2 H), 7.61 (t, 1 H), 7.24 (d, 1 H), 6.75 (br. s., 1 H), 6.58 (d, 1 H). MS ES+ m/z 355 [M+H] ⁺ .

Intermediate example 5

4-[2-tert-Butoxy-6-(3-methyl-4-pyridyl)-4-pyridyl]-1 H-pyrrolo[2,3- b]pyridine

The title compound was prepared as described in Intermediate example 3, using (3-methyl-4-pyridyl)boronic acid and Pd(amphos)Cl2, to give the product (125 mg, 53%). MS ES+ m/z 359 [M+H] ⁺ .

Example 6

6-(3-Methyl-4-pyridyl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 4, using 4-[2-tert- butoxy-6-(3-methyl-4-pyridyl)-4-pyridyl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (70 mg, 66%). ¹ H NMR (400MHz, DMSO-cfe) δ = 1 1 .94 (br. s., 1 H), 1 1 .78 (br. s., 1 H), 8.57 (s., 1 H), 8.52 (s., 1 H), 8.31 (s., 1 H), 7.61 (s., 1 H), 7.47 (s.,1 H), 7.29 (s., 1 H), 6.77 (s., 1 H), 6.70 (s., 1 H), 6.63 (s., 1 H), 2.38 (s., 3 H). MS ES+ m/z 303 [M+H] ⁺ .

Intermediate example 7

4-[2-tert-Butoxy-6-(2-phenylpyrrolidin-1 -yl)-4-pyridyl]-1 H-pyrrolo[2,3- b]pyridine

4-(2-tert-Butoxy-6-chloro-4-pyridyl)-1 H-pyrrolo[2,3-b]pyridine (134 mg, 0.44 mmol) and 2-phenylpyrrolidine (131 mg, 0.89 mmol) were dissolved in toluene (5 ml). Pd ₂ (dba) ₃ (23 mg, 0.03 mmol), Xphos (23 mg, 0.05 mmol) and KOtBu (160 mg, 1 .43 mmol) were added and the suspension was stirred at 100 °C for 2 h. Brine and EtOAc were added, the organic layer separated and the aqueous phase extracted with EtOAc. The combined organics were washed with 2 M aq. HCI, water, brine, dried over MgSO ₄ , filtered and concentrated to give the product (92 mg, 50%). MS ES+ m/z 413 [M+H] ⁺ .

Example 8

6-(2-phenylpyrrolidin-1 -yl)-4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2- one

The title compound was prepared as described in Example 4, using 4-[2-tert- butoxy-6-(2-phenylpyrrolidin-1 -yl)-4-pyridyl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (6 mg, 8%). ¹ H NMR (400 MHz, DMSO-cfe) δ ppm 1 .84 (br. s., 1 H) 1 .94 (br. s., 2 H) 2.35 - 2.45 (m, 1 H) 3.66 (d, 1 H) 3.86 (br. s., 1 H) 4.98 (br. s., 1 H) 5.72 - 6.01 (m, 2 H) 7.02 (d, 1 H) 7.17 - 7.41 (m, 7 H) 8.20 (d, 1 H) 1 1 .73 (br. s., 1 H). MS ES+ m/z 357 [M+H] ⁺ .

Intermediate example 9

2-Methoxy-6-(3-pyridyl)pyridin-4-amine

2-Chloro-6-methoxy-pyridin-4-amine (2 g, 12.61 mmol), 3-pyridylboronic acid (1 .55 g, 0.01 mol), Pd(amphos)CI ₂ (0.73 g, 0.63 mmol) and K ₂ CO ₃ (3.49 g, 0.03 mol) were dissolved in MeCN (6 ml) and water (2 ml) and the mixture was heated in the microwave reactor at 130 °C for 30 min. The mixture was filtered and the organic layer was evaporated. The resulting residue was dissolved in EtOAc, washed with water, brine, dried over MgSO ₄ and concentrated to give the product as a solid (1 .2 g 47%). MS ES+ m/z 202 [M+H] ⁺ .

Intermediate example 10

4-Chloro-2-methoxy-6-(3-pyridyl)pyrid

2-Methoxy-6-(3-pyridyl)pyridin-4-annine (1 g, 4.92 mmol) and chlorocopper (779 mg, 7.87 mmol) were suspended in acetonitrile (25 ml) and cooled in an ice-bath. Isopentyl nitrite (1 .06 ml, 7.87 mmol) was added and the mixture was stirred at rt overnight. Water (60 ml) was added and the mixture extracted with ethyl acetate (2 x 20 mL). The combined organics was washed with brine (20 ml), dried over MgSO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-30% EtOAc in heptane to give the product as a solid (403 mg, 37%). MS ES+ m/z 222 [M+H] ⁺ .

Intermediate example 11

1 -(Benzenesulfonyl)-4-[2-methoxy-6-(3-pyridyl)-4-pyridyl]-2-m ethyl- pyrrolo[2,3-b]pyridine

4-Chloro-2-methoxy-6-(3-pyridyl)pyridine (90 mg, 0.41 mmol), 1 -

(benzenesulfonyl)-2-methyl-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2- yl)pyrrolo[2,3-b]pyridine (187 mg, 0.47 mmol), PdCI ₂ (PPh ₃ )2 (14 mg, 0.02 mmol) and K2CO3 (141 mg, 1 .02 mmol) were taken up in MeCN (3 ml) and water (0.5 ml) and the resulting mixture was heated in a microwave reactor at 140 °C for 15 min. More 1 -(benzenesulfonyl)-2-methyl-4-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolan-2-yl)pyrrolo[2,3-b]pyridine (120 mg, 0.3 mmol) was added and the mixture was heated again at 140 °C for 15 min. The mixture was filtered and the filtrate concentrated. The resulting residue was taken up in EtOAc (10 ml) and water (10 ml). The organic layer was separated and the aqueous layer extracted with EtOAc (10 ml). The combined organics were washed with brine, dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-80% EtOAc in heptane to give the product (182 mg, 55%). MS ES+ m/z 457 [M+H] ⁺ .

Example 12

4-(2-Methyl-1 H^yrrolo[2,3^]pyridin-4-yl)-6-(3^yridyl)-1 H-pyridin-2-one

1 -(Benzenesulfonyl)-4-[2-methoxy-6-(3-pyridyl)-4-pyridyl]-2-m ethyl- pyrrolo[2,3-b]pyridine (287 mg, 0.63 mmol) was taken up in 1 ,4-dioxane (2 ml) and 2M aq. HCI (2 ml) was added. The resulting mixture was heated in a microwave reactor at 130 °C for 1 .5 h. Cone HCI (0.5 ml) was added and the mixture was heated again at 140 °C for 1 h. When cooled to rt the pH was adjusted >7 using 2M aq NaOH and the formed precipitate was filtered off, washed with water, EtOAc and dried. The crude product was taken up in MeOH (4 ml) and heated in a microwave reactor at 150 °C for 3 min. When cooled to rt the resulting precipitate was filtered off, washed sequentially with MeOH, 2-propanol, pentane and dried to give the product as a solid (65 mg, 34%). ¹ H NMR (500MHz ,DMSO-c/ ₆ ) δ = 12.03 (br. s., 1 H), 1 1 .76 (br. s., 1 H), 9.1 1 (br. s., 1 H), 8.66 (d, 1 H), 8.30 (d, 1 H), 8.20 (d, 1 H), 7.59 - 7.48 (m, 1 H), 7.29 (d, 1 H), 7.20 (br. s., 1 H), 6.80 (br. s., 1 H), 6.40 (s, 1 H), 2.44 (s, 3 H). MS ES+ m/z 303 [M+H] ⁺ . Intermediate example 13

4-(6-Chloro-4-iodo-2-pyridyl)morpholine

2,6-Dichloro-4-iodo-pyridine (1 .37 g, 5 mmol), morpholine (0.56 ml, 6.5 mmol), L-proline (1 15 mg, 1 mmol), Cul (95 mg, 0.5 mmol) and K2CO3 (1 .38 g, 10 mmol) were taken up in DMSO (4 ml) and the resulting mixture was stirred at 70 °C overnight. When cooled to rt water (25 ml) and EtOAc (10 ml) were added and the organic layer separated. The aqueous layer was extracted with EtOAc (2 x 10 ml). The combined organics were washed with brine, dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 20-50% EtOAc in heptane to give the product as a solid (865 mg, 53%). MS ES+ m/z 325 [M+H] ⁺ .

Intermediate example 14

4-[4-[1 -(Benzenesulfonyl)-2-methyl-pyrrolo[2,3-b]pyridin-4-yl]-6-ch loro-2- pyridyl]morpholine

1 -(Benzenesulfonyl)-2-methyl-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2- yl)pyrrolo[2,3-b]pyridine (518 mg, 1 .3 mmol), 4-(6-chloro-4-iodo-2- pyridyl)morpholine (325 mg, 1 mmol), Pd(PP i3)4 (58 mg, 0.05 mmol) and K2CO3 (415 mg, 3 mmol) were taken up in 1 ,4-dioxane:H ₂ O:EtOH (6:3:1 , 10 ml) and the resulting mixture was stirred at 80 °C for 2 h. When cooled to rt EtOAc (5 ml) and brine (5 ml) were added. The organic layer was separated and the aqueous layer extracted with EtOAc (5 ml). The combined organics were dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-50% EtOAc in heptane to give the product as a solid (470 mg, quant). MS ES+ m/z 470 [M+H] ⁺ .

Intermediate example 15

4-[6-Chloro-4-(2-methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-2- pyridyl]morpholine

4-[4-[1 -(Benzenesulfonyl)-2-methyl-pyrrolo[2,3-b]pyridin-4-yl]-6-ch loro-2- pyridyl]morpholine (335 mg, 0.71 mmol), (2,4-dimethoxyphenyl)methanol (144 mg, 0.86 mmol), KOtBu (400 mg, 3.57 mmol) and toluene (1 ml) were mixed and stirred at 90 °C for 3 h. When cooled to rt EtOAc (10 ml), water (5 ml) and brine (5 ml) were added. The organic layer was separated and the aqueous layer extracted with EtOAc (2 x 5 ml). The combined organics were washed with brine, dried over Na2SO ₄ , filtered and concentrated to give the product as a solid (300 mg, 64%). MS ES+ m/z 330 [M+H] ⁺ .

Example 16

4-(2-Methyl-1 H^yrrolo[2,3^]pyridin-4-yl)-6-morpholino-1 H-pyridin-2-one

4-[6-Chloro-4-(2-methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-2-pyridyl]morpholine (300 mg, 0.46 mmol), Pd ₂ (dba) ₃ (21 mg, 0.02 mmol), XPhos (22 mg, 0.05 mmol), 2M aq. KOH (1 ml) and 1 ,4-dioxane (2 ml) were mixed and heated in a microwave reactor at 150 °C for 30 min. When cooled to rt the mixture was diluted with EtOAc and water. The organic layer was separated and the aqueous layer was extracted with EtOAc. The combined organics were washed with brine, dried over Na2SO ₄ , filtered and purified by preparative HPLC to give the product as a solid (4 mg, 3%). ¹ H NMR (500MHz, DMSO- Gfe) δ = 1 1 .66 (br. s., 1 H), 10.43 (br. s., 1 H), 8.14 (d, 1 H), 7.13 (d, 1 H), 6.38 (br. s., 1 H), 6.31 (s, 1 H), 6.22 (br. s., 1 H), 3.75 - 3.66 (m, 4 H), 3.43 (br. s., 4 H), 2.42 (s, 3 H). MS ES+ m/z 31 1 [M+H] ⁺ . Intermediate example 17

6-(2-Chlorophenyl)-4-hydroxy-1 H-pyridin-2-one

Ethyl 3-oxobutanoate (6.33 ml, 50 mmol) was added dropwise to a

suspension of 60% NaH (1 .92 g, 50 mmol) in 2-MeTHF (60 ml) at -78 °C under a nitrogen atmosphere. After 5 min the cooling bath was removed and the mixture was stirred at rt for 20 min. The mixture was cooled back to - 78 °C and 1 .6 M n-BuLi (31 .25 ml) was added slowly over 20 min. The resulting solution was stirred at -78 °C for 30 min. Then 2-chlorobenzonitrile (6.88 g, 50 mmol) was added as a solid in one portion and the reaction mixture was stirred on the thawing cooling bath overnight. The mixture was cooled to 0 °C and MeOH (15 ml) was added slowly. The cooling bath was removed and the mixture was stirred at rt for 30 min and then cooled to 0 °C again. The mixture was neutralized by slow addition of cone. HCI and the resulting precipitate was filtered off, washed with EtOH, pentane and dried to give the product as a solid (1 1 .08 g, 87%). MS ES+ m/z 222 [M+H] ⁺ . Intermediate example 18

2,4-Dichloro-6-(2-chlorophenyl)pyrid

6-(2-Chlorophenyl)-4-hydroxy-1 H-pyridin-2-one (5 g, 22.56 mmol) was taken up in POCI3 (40 ml) and N,N-dimethylaniline (5.5 ml, 43.4 mmol) was added slowly. The resulting mixture was refluxed overnight. When cooled to rt the mixture was poured onto ice (600 ml) and stirred at rt for 30 min. The precipitate was filtered off and washed with water. The solid was dissolved in EtOAc (100 ml), dried over Na2SO ₄ , filtered and concentrated to give the product as a solid (7 g, 83%). MS ES+ m/z 258 [M+H] ⁺ .

Intermediate example 19

4-Chloro-6-(2-chlorophenyl)-1 H-pyridin-2-one

2,4-Dichloro-6-(2-chlorophenyl)pyridine (5.7 g, 22.05 mmol) and KOtBu (6.19 g, 55.12 mmol) were taken up in toluene (75 ml) and the resulting mixture was stirred at 100 °C for 2 h. When cooled to rt, water (40 ml) was added and the organic layer separated. The aqueous layer was made slightly acidic using cone. HCI and extracted with EtOAc (2 x 40 ml). The combined organics were washed with brine (50 ml), dried over Na2SO ₄ , filtered and concentrated. The resulting residue was taken up in DCM (30 ml) and TFA (5 ml, 67.3 mmol) was added. The reaction mixture was stirred at rt for 1 h, concentrated and the resulting residue was taken up in MeOH (25 ml). 30% NH ₄ OH (20 ml) and water (20 ml) were added and the mixture was stirred at rt overnight. The formed precipitate was filtered off, washed with water, EtOH, pentane and dried to give the product as a solid (4.13 g, 78%). MS ES+ m/z 240 [M+H] ⁺ .

Intermediate example 20

1 -(Benzenesulfonyl)-4-chloro-pyrrolo[2,3-b]pyridine-2-carbald ehyde

2.5M Butyllithium (2.8 ml, 7 mmol) was added slowly to a solution of N- isopropylpropan-2-amine (1 ml, 7.14 mmol) in 2-MeTHF (15 ml) at 0 °C. The resulting mixture was stirred at 0 °C for 5 min and was then cooled to -78 °C A solution of 1 -(benzenesulfonyl)-4-chloro-pyrrolo[2,3-b]pyridine (1 .5 g, 5.12 mmol) in 2-MeTHF (10 ml) was added dropwise. The resulting mixture was stirred at -78 °C for 20 min then the cooling bath was removed. The mixture was stirred at rt for 10 min to give a suspension and then cooled to -78 °C again. DMF (1 .2 ml, 15.6 mmol) was added dropwise and the resulting mixture was stirred on the thawing cooling bath for 30 min and then at rt for 30 min. Sat. aq. NH ₄ CI (12 ml) was added and the organic layer separated. The aqueous layer was extracted with EtOAc (2 x 10 ml) and the combined organics were washed with 0.5 M aq. HCI (20 ml), brine (15 ml), dried over Na2SO ₄ , filtered and concentrated to give the product as a gum (1 .7 g, quant.). MS ES+ m/z 321 [M+H] ⁺ .

Intermediate example 21

5-(4-Chloro-1 H-pyrrolo[2,3-b]pyridin-2-yl)oxazole

1 -(Benzenesulfonyl)-4-chloro-pyrrolo[2,3-b]pyridine-2-carbald ehyde (1 g, 3.12 mmol), TosMIC (609 mg, 3.12 mmol) and K ₂ CO ₃ (431 mg, 3.12 mmol) were taken up in MeOH (40 ml) and the resulting mixture was stirred at 70 °C for 2 h. When cooled to rt the mixture was filtered and the precipitate washed with MeOH. The filtrate was concentrated and the resulting residue was

recrystallized from EtOH to give the product as a solid (120 mg, 18%). MS ES+ m/z 220 [M+H] ⁺ . Intermediate example 22

S-^^^^^-Tetramethyl-l ^^-dioxaborolan^-y -I H-pyrrolo^^- b]pyridin-2-yl]oxazole

5-(4-Chloro-1 H-pyrrolo[2,3-b]pyridin-2-yl)oxazole (120 mg, 0.55

mmol), 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)- 1 ,3,2-dioxaborolane (208 mg, 0.82 mmol) and KOAc (161 mg, 1 .64 mmol) were taken up in 1 ,4-dioxane (10 ml) and nitrogen was bubbled through the mixture for 5 min. Then S-Phos (22 mg, 0.06 mmol) and Pd(OAc)2 (6 mg, 0.03 mmol) were added and the resulting mixture was stirred at 100 °C overnight. More 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan- 2-yl)-1 ,3,2-dioxaborolane (208 mg, 0.82 mmol), KOAc (161 mg, 1 .64 mmol), S-Phos (22 mg, 0.06 mmol) and Pd(OAc) ₂ (6 mg, 0.03 mmol) were added and the resulting mixture was stirred at 100 °C overnight. Pd-1 18 (25 mg) was added and stirring was continued at 100 °C overnight. When cooled to rt the mixture was filtered and to the filtrate was added water (10 ml), brine (10 ml) and EtOAc (10 ml). The organic layer was separated and the aqueous layer extracted with EtOAc (2 x 10 ml). The combined organics were dried over Na2SO ₄ , concentrated and purified on a silica gel column eluted with 0-10% MeOH in DCM to give the product as a solid (160 mg, 66%). MS ES+ m/z 312 [M+H] ⁺ .

Example 23

6-(2-Chlorophenyl)-4-(2-oxazol-5-yl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H- pyridin-2-one

5-[4-(4,4,5,5-Tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-pyrrolo[2,3-b]pyridin-2- yl]oxazole (160 mg, 0.36 mmol), 4-chloro-6-(2-chlorophenyl)-1 H-pyridin-2-one (75 mg, 0.31 mmol), PdCI ₂ (PPh ₃ )2 (1 1 mg, 0.02 mmol) and K ₂ CO ₃ (130 mg, 0.94 mmol) were taken up in 1 ,4-dioxane:H2O:EtOH (6:3:1 ; 3 ml) and the resulting mixture was heated in a microwave reactor at 140 °C for 15 min. When cooled to rt water (2 ml) was added and the mixture was stirred at rt for 15 min. The resulting precipitate was filtered off, washed sequentially with water, 1 ,4-dioxane and dried. The crude product was suspended in boiling 2- propanol (5 ml). When cooled to rt the precipitate was filtered off and discarded. The filtrate was concentrated and the resulting residue was suspended in a small amount of EtOH, filtered and concentrated to give the product as a solid (1 1 mg, 9%). MS ES+ m/z 389 [M+H] ⁺ . Intermediate example 24

1 -(Benzenesulfonyl)-4-chloro-2-(3-pyridyl)pyrrolo[2,3-b]pyrid ine

1 -(Benzenesulfonyl)-4-chloro-2-iodo-pyrrolo[2,3-b]pyridine (2.97 g, 7.09 mmol), 3-pyridylboronic acid (0.87 g, 7.09 mmol), Pd(PPh ₃ )4 (0.41 g, 0,35 mmol) and K2CO3 (1 .96 g, 14.2 mmol) were dissolved in MeCN (6 ml) and water (2 ml) and the mixture was heated in a microwave reactor at 130 °C for 30 min. The mixture was filtered and the organic layer evaporated. The resulting residue was dissolved in EtOAc, washed with water, brine, dried over MgSO ₄ , concentrated and purified on a silica gel column eluted with 50% EtOAc in heptane to give the product as a solid (500 mg, 19%). MS ES+ m/z 370 [M+H] ⁺ .

Intermediate example 25

1 -(Benzenesulfonyl)-2-(3-pyridyl)-4-(4,4,5,5-tetramethyl-1 ,3,2- dioxaborolan-2-yl)pyrrolo[2,3-b]pyridine

The title compound was prepared as described in Intermediate example 22, using 1 -(benzenesulfonyl)-4-chloro-2-(3-pyridyl)pyrrolo[2,3-b]pyrid ine instead of 5-(4-Chloro-1 H-pyrrolo[2,3-b]pyridin-2-yl)oxazole, to give the product (100 mg, 16%). MS ES+ m/z 462 [M+H] ⁺ .

Intermediate example 26

4-[1 -(Benzenesulfonyl)-2-(3-pyridyl)pyrrolo[2,3-b]pyridin-4-yl]- 6-(2- chlorophenyl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 23, using 1 - (benzenesulfonyl)-2-(3-pyridyl)-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2- yl)pyrrolo[2,3-b]pyridine instead of 5-[4-(4,4,5,5-Tetramethyl-1 ,3,2- dioxaborolan-2-yl)-1 H-pyrrolo[2,3-b]pyridin-2-yl]oxazole. Purification on a silica gel column eluted with 0-10% MeOH in DCM gave the product as a solid (140 mg, 78%). MS ES+ m/z 462 [M+H] ⁺ .

Example 27

6-(2-Chlorophenyl)-4-[2-(3-pyridyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-1 H- pyridin-2-one

o

4-[1 -(Benzenesulfonyl)-2-(3-pyridyl)pyrrolo[2,3-b]pyndin-4-yl]-6 -(2- chlorophenyl)-1 H-pyridin-2-one (140 mg, 0.26 mmol) was taken up in 1 ,4- dioxane (2 ml) and 2M aq. HCI (1 ml) and the resulting mixture was heated in a microwave reactor at 130 °C for 30 min. 2M aq. NaOH (1 ml) and water (3 ml) were added and the resulting precipitate was filtered off and washed with water. The crude product was taken up in boiling 2-propanol (5 ml) and when cooled to rt the precipitate was filtered off and dried to give the product as a solid (15 mg, 15%). ¹ H NMR (400MHz, DMSO-cfe) δ = 12.59 (br. s., 1 H), 9.27 - 9.19 (m, 1 H), 8.56 (d, 1 H), 8.41 - 8.30 (m, 2 H), 7.72 - 7.58 (m, 2 H), 7.57 - 7.43 (m, 4 H), 7.35 - 7.24 (m, 2 H), 6.81 (d, 1 H). MS ES+ m/z 399 [M+H] ⁺ .

Intermediate example 28

4-[1 -(Benzenesulfonyl)-2-methyl-pyrrolo[2,3-b]pyridin-4-yl]-6-(2 - chlorophenyl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 23, using 1 - (benzenesulfonyl)-2-methyl-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2- yl)pyrrolo[2,3-b]pyridine instead of 5-[4-(4,4,5,5-Tetramethyl-1 ,3,2- dioxaborolan-2-yl)-1 H-pyrrolo[2,3-b]pyridin-2-yl]oxazole. Purification on a silica gel column eluted with 50-100% EtOAc in heptane gave the product a solid (140 mg, 88%). MS ES+ m/z 476 [M+H] ⁺ .

Example 29

6-(2-Chlorophenyl)-4-(2-methyl-1 H^yrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin- 2-one

The title compound was prepared as described in Example 27, using 4-[1 - (benzenesulfonyl)-2-methyl-pyrrolo[2,3-b]pyridin-4-yl]-6-(2- chlorophenyl)-1 H- pyridin-2-one instead of 4-[1 -(Benzenesulfonyl)-2-(3-pyridyl)pyrrolo[2,3- b]pyridin-4-yl]-6-(2-chlorophenyl)-1 H-pyridin-2-one, to give the product as a solid (25 mg, 25%). ¹ H NMR (400MHz, DMSO-cfe) δ = 12.03 (br. s., 1 H), 1 1 .75 (br. s., 1 H), 8.18 (d, 1 H), 7.65 - 7.58 (m, 2 H), 7.54 - 7.42 (m, 3 H), 7.20 (d, 1 H), 6.71 (br. s., 1 H), 6.35 (s, 1 H), 2.43 (s, 3 H). MS ES+ m/z 336 [M+H] ⁺ .

Intermediate example 30

4-Benzyloxy-2,6-dichloro-pyridine

60% NaH (945 mg, 24.7 mmol) was added portion wise to a solution of 2,4,6- trichloropyridine (4.5 g, 24.7 mmol) in DMF (25 ml) at 0°C. After 20 min phenylmethanol (2.7 g, 24.7 mmol) was added dropwise and the mixture was stirred for 3h. Water (30 ml) was added and the precipitate was filtered off. The solid was dissolved in EtOAc, dried over MgSO ₄ , filtered and

concentrated to give the product as a solid (5 g, 80%). MS ES+ m/z 254

[M+H] ⁺ .

Intermediate example 31

4-Benzyloxy-2-tert-butoxy-6-chloro-pyridine

4-Benzyloxy-2,6-dichloro-pyridine (5 g, 19.7 mmol) and KOtBu (2.2 g, 19.7 mmol) were dissolved in 2-MeTHF (25 ml) and the mixture was stirred at 70 °C for 2h. When cooled to rt the mixture was filtered, concentrated and purified on a silica gel column eluted with 30% EtOAc in heptane to give the product (4 g, 70%). MS ES+ m/z 292 [M+H] ⁺ .

Intermediate example 32

4-Benzyloxy-2-tert-butoxy-6-[2-(trifluoromethyl)-1 -piperidyl]pyridine

4-Benzyloxy-2-tert-butoxy-6-chloro-pyhdine (4 g, 13.7mmol), 2- (trifluoromethyl)piperidine (2.3 g, 15.1 mmol), PEPPSI-iPr (146 mg, 1 .37mmol) and KOtBu (3.85 g, 34.3 mmol) were taken up in 1 ,4-dioxane (30 ml) and the mixture was stirred at 90 °C for 2h. When cooled to rt water and EtOAc were added and the organic layer separated, filtered, concentrated and purified on silica gel column eluted with 30% EtOAc in heptane to give the product (4.1 g, 73%). MS ES+ m/z 409 [M+H] ⁺ .

Intermediate example 33

2-tert-Butoxy-6-[2-(trifluoromethyl)-1 -piperidyl]pyridin-4-ol

A mixture of 4-benzyloxy-2-tert-butoxy-6-[2-(trifluoromethyl)-1 - piperidyl]pyridine (3.5 g, 8.57 mmol) and 10% Pd/C (600 mg, 0.56 mmol) in MeOH and EtOAc was hydrogenated (1 .5 bar) at rt for 2 h. The mixture was filtered through celite and concentrated to give the product (2.7 g, quant.). MS ES+ m/z 319 [M+H] ⁺ .

Intermediate example 34

[2-tert-Butoxy-6-[2-(trifluoromethyl)-1 -piperidyl]-4-pyridyl]

trifluoromethanesulfonate

2-tert-Butoxy-6-[2-(thfluoromethyl)-1 -piperidyl]pyridin-4-ol (2.7 g, 8.48 mmol) and Et ₃ N (1 .66 ml, 1 1 .9 mmol) was taken up in DCM (20 ml_) at 0°C.

Trifluoromethylsulfonyl trifluoromethanesulfonate (2.54 ml, 1 1 .9 mmol) was added dropwise over 5 minutes and stirred for 1 h. The mixture was washed with sat. aq. NaHCO3 (2* 20 ml_), concentrated and purified on silica gel column eluted with 20% EtOAc in heptane to give the product (3.5 g, 92%). MS ES+ m/z 451 [M+H] ⁺ . Intermediate example 35

2-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2- (trifluoromethyl)-1 -piperidyl]pyridine

4,4,5,5-Tetramethyl-2-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 ,3,2- dioxaborolane (2.96 g, 1 1 .7 mmol), [2-tert-butoxy-6-[2-(trifluoromethyl)-1 - piperidyl]-4-pyridyl] trifluoromethanesulfonate (3.5 g, 7.77 mmol), KOAc (1 .14 g, 1 1 .7 mmol) and PdCl2(dppf) (215 mg, 0.29 mmol) were taken up in toluene (10 ml) and stirred at 90 °C for 5 h. When cooled to rt the mixture was concentrated and the residue dissolved in EtOAc, washed with water, concentrated and purified on a silica gel column eluted with 0-60% EtOAc in heptane to give the product (2.15 g, 65%). MS ES+ m/z 347 [M+H] ⁺ .

Example 36

4-(2-Methyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 - piperidyl]-1 H-pyridin-2-one

2-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2- (trifluoromethyl)-1 -piperidyl]pyridine (1 10 mg, 0.26 mmol), 4-chloro-2-methyl- 1 H-pyrrolo[2,3-b]pyridine (95 mg, 0.57 mmol), K ₂ CO ₃ (89 mg, 0.64 mmol) and PdCl2(dppf) (14 mg, 0.02) were taken up in 1 ,4-dioxane and water and the mixture was stirred at 90 °C for 2h. When cooled to rt EtOAc was added and the mixture filtered. The organic layer was separated, dried over MgSO ₄ and concentrated. The resulting residue was dissolved in DCM, TFA (0.1 1 ml, 1 .54 mmol) was added, the mixture stirred at rt for 30 min, concentrated and purified by preparative HPLC to give the product as a solid (3.4 mg, 3 %). ¹ H NMR (500 MHz, METHANOL-^) δ ppm 1 .62 - 1 .70 (m, 1 H), 1 .76 - 1 .94 (m, 4 H), 2.07 - 2.16 (m, 1 H), 2.51 (d, 3 H), 3.19 - 3.28 (m, 1 H), 3.85 - 4.06 (m, 1 H), 5.37 (br d, 1 H), 6.32 (d, 1 H), 6.36 (d, 1 H), 6.39 - 6.47 (m, 1 H), 7.17 (d, 1 H), 8.06 - 8.27 (m, 1 H). MS ES+ m/z 377 [M+H] ⁺ .

Example 37

4-(1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -piperidyl]-1 H- pyridin-2-one

4-Chloro-1 H-pyrrolo[2,3-b]pyridine (43 mg, 0.28 mmol), 2-tert-butoxy-4- (4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2-(trifluoromethyl)-1 - piperidyl]pyridine (100 mg, 0.23 mmol), K2CO3 (81 mg, 0.58 mmol) and PdCl2(dppf) (13 mg, 0.02 mmol) were taken up in 1 ,4-dioxane and water and the mixture was heated in a microwave reactor at 120 °C for 1 h. When cooled to rt EtOAc was added and the mixture filtered. The organic layer was separated, dried over MgSO ₄ , concentrated and purified by preparative HPLC to give the product as a solid (3.4 mg, 4%). ¹ H NMR (500 MHz, METHANOL- c/ ₄ ) δ ppm 1 .57 - 1 .69 (m, 1 H), 1 .77 - 1 .95 (m, 4 H), 2.07 - 2.18 (m, 1 H), 3.20 - 3.31 (m, 1 H), 3.98 (br d, 1 H), 5.27 - 5.57 (m, 1 H), 6.34 (d, 1 H), 6.46 (s, 1 H), 6.67 (d, 1 H), 7.28 (s, 1 H), 7.49 (d, 1 H), 8.15 - 8.39 (m, 1 H). MS ES+ m/z 363 [M+H] ⁺ . Intermediate example 38

4-(4-Benzyloxy-6-tert-butoxy-2-pyridyl)-3-(trifluoromethyl)m orpholine

The title compound was prepared as described in Intermediate example 32, using 3-(trifluoromethyl)morpholine instead of 2-(trifluoromethyl)piperidine, to give the product as an oil (1 g, 50%). MS ES+ m/z 41 1 [M+H] ⁺ .

Intermediate example 39

2-tert-Butoxy-6-[3-(trifluoromethyl)morpholin-4-yl]pyridin-4 -ol

The title compound was prepared as described in Intermediate example 33 to give the product (780 mg, 99%). MS ES+ m/z 321 [M+H] ⁺ .

Intermediate example 40

[2-tert-Butoxy-6-[3-(trifluoromethyl)morpholin-4-yl]-4-pyrid yl]

trifluoromethanesulfonate

The title compound was prepared as described in Intermediate example 34 to give the product as an oil (800 mg, 81 %). MS ES+ m/z 453 [M+H] ⁺ .

Intermediate example 41

4-[6-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2- pyridyl]-3-(trifluoromethyl)morpholine

The title compound was prepared as described in Intermediate example 35 to give the product (270 mg, 33%). MS ES+ m/z 431 [M+H] ⁺ .

Example 42

4-(1 H^yrrolo[2,3-b]pyridin-4-yl)-6-[3-(trifluoromethyl)morpholin -4-yl]-1 H- pyridin-2-one

The title compound was prepared as described in Example 36, using 4-[6-tert- Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-3- (trifluoromethyl)morpholine and 4-chloro-1 H-pyrrolo[2,3-b]pyridine, to give the product (20 mg, 22%). ¹ H NMR (500 MHz, METHANOL- c/ ₄ ) δ ppm 3.44 - 3.51 (m, 1 H), 3.63 (td, 2.99 Hz, 1 H), 3.72 - 3.83 (m, 2 H), 3.99 (dd, 1 H), 4.26 (d, 1 H), 5.18 (qd, 1 H), 6.35 - 6.39 (m, 1 H), 6.37 (s, 1 H), 6.41 (s, 1 H), 6.65 (d, 1 H), 7.20 (d, 1 H), 7.43 (d, 1 H), 8.23 (d, 1 H). MS ES+ m/z 365

[M+H] ⁺ .

Intermediate example 43

1 -(Benzenesulfonyl)-4-chloro-2-[3-(trifluoromethyl)phenyl]pyr rolo[2,3- b]pyridine

The title compound was prepared as described in Intermediate example 24, starting from [3-(trifluoromethyl)phenyl]boronic acid, to give the product (1 .5 g, 29%). MS ES+ m/z 437 [M+H] ⁺ .

Intermediate example 44

4-Chloro-2-[3-(trifluoromethyl)phenyl]-1 H-pyrrolo[2,3-b]pyridine

5M NaOH in MeOH (1 .37 ml, 6.88 mmol) was added to a solution of 1 -

(benzenesulfonyl)-4-chloro-2-[3-(trifluoromethyl)phenyl]p yrrolo[2,3-b]pyridine (1 .5 g, 3.44 mmol) in MeOH (10 ml) and the resulting mixture was stirred at rt for 1 h. Water was added and the mixture extracted with EtOAc. The combined organics were dried over Na2SO ₄ , filtered and purified on a silica gel column to give the product as a solid (510 mg, 50%). MS ES+ m/z 297 [M+H] ⁺ . Example 45

6-[3-(Trifluoromethyl)morpholin-4-yl]-4-[2-[3-(trifluorometh yl)ph pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 4-[6-tert- Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-3- (trifluoromethyl)morpholine and 4-chloro-2-[3-(trifluoromethyl)phenyl]-1 H- pyrrolo[2,3-b]pyridine, to give the product (7 mg, 7%). ¹ H NMR (500 MHz, METHANOL- c/ ₄ ) δ ppm 3.46 - 3.53 (m, 1 H), 3.67 (td, 3.00 Hz, 1 H), 3.81 - 3.93 (m, 2 H), 4.03 (dd, 1 H), 4.30 (d, 1 H), 5.21 - 5.28 (m, 1 H), 6.46 (d, 1 H), 6.54 (s, 1 H), 7.14 (s, 1 H), 7.28 (d, 1 H), 7.66 - 7.70 (m, 2 H), 8.14 - 8.18 (m, 1 H), 8.21 (s, 1 H), 8.31 (d, 1 H). MS ES+ m/z 509 [M+H] ⁺ .

Intermediate example 46

1 -(Benzenesulfonyl)-4-chloro-2-(5-methyl-2-thienyl)pyrrolo[2, 3- b]pyridine

A mixture of tributyl-(5-methyl-2-thienyl)stannane (6 g, 15.46 mmol), 1 - (benzenesulfonyl)-4-chloro-2-iodo-pyrrolo[2,3-b]pyridine (5.1 g, 12.88 mmol), Pd(PPh ₃ )4 (1 .7 g, 0.64 mmol) and 2M aq. Na ₂ CO ₃ (12 mL) in 1 ,4-dioxane (25 mL) was purged with argon and stirred at 100 °C overnight. When cooled to rt, the mixture was diluted EtOAc, washed with water, brine, dried over MgSO ₄ , concentrated and purified on a silica gel column to give the product as a solid (2 g, 33%). MS ES+ m/z 389 [M+H] ⁺ .

Intermediate example 47

4-Chloro-2-(5-methyl-2-thienyl)-1 H-pyrrolo[2,3-b]pyridine

The title compound was prepared as described in Intermediate example 44, starting from 1 -(benzenesulfonyl)-4-chloro-2-(5-methyl-2-thienyl)pyrrolo[2, 3- b]pyridine, to give the product (835 mg, 59%). MS ES+ m/z 249 [M+H] ⁺ .

Example 48

4-[2-(5-Methyl-2-thienyl)-1 H-pyrrolo[2,3-b]pyridin-4-yl]-6-[3- (trifluoromethyl)morpholin-4-yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 4-[6-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-3- (trifluoromethyl)morpholine and 4-chloro-2-(5-methyl-2-thienyl)-1 H- pyrrolo[2,3-b]pyridine, to give the product (5 mg, 5%). ¹ H NMR (500 MHz, METHANOL- c/ ₄ ) δ ppm 2.51 (s, 3 H), 3.43 - 3.55 (m, 1 H), 3.64 (br d, 1 H), 3.73 (br s, 1 H), 3.81 (br d, 1 H), 4.02 (br d, 1 H), 4.28 (br d, 1 H), 5.1 1 (br s, 1 H), 6.40 (br d, 2 H), 6.65 - 6.71 (m, 1 H), 6.76 (br s, 1 H), 7.12 - 7.16 (m, 1 H), 7.25 - 7.31 (m, 1 H), 8.17 (br d, 1 H). MS ES+ m/z 461 [M+H] ⁺ . Example 49

4-(1 H-pyrazolo[3,4-b]pyridin-4-yl)-6-[2-(trifluoromethyl)-1 -piperidyl]-1 H- pyridin-2-one

The title compound was prepared as described in Example 36, using 4- chloro-1 H-pyrazolo[3,4-b]pyridine, to give the product (6 mg, 7%). ¹ H NMR (500 MHz, METHANOL- c/ ₄ ) δ ppm 1 .65 (br d, 1 H), 1 .78 (br s, 1 H), 1 .80 - 1 .91 (m, 3 H), 2.12 (br d, 1 H), 3.24 (br t, 1 H), 4.07 (br d, 1 H), 5.47 (br s, 1 H), 6.38 (s, 1 H), 6.53 (s, 1 H), 7.33 - 7.48 (m, 1 H), 8.26 (s, 1 H), 8.61 (d, 1 H). MS ES+ m/z 461 [M+H] ⁺ .

Intermediate example 50

2-[[4-Chloro-2-[2-(trifluoromethyl)pyrimidin-5-yl]pyrrolo[2, 3-b]pyridin-1 - yl]methoxy]ethyl-trimethyl-silane

A mixture of [4-chloro-1 -(2-trimethylsilylethoxymethyl)pyrrolo[2,3-b]pyridin-2- yl]boronic acid (2 g, 12.26 mmol), 5-bromo-2-(trifluoromethyl)pyrimidine (2.6 g, 12.26 mmol), Pd(PPh ₃ )4 (1 .4 g, 1 .22 mmol) and 2M aq. Na ₂ CO ₃ (2 ml_) in 1 ,4-dioxane (30 ml_) was purged with argon and stirred at 100 °C overnight. When cooled to rt the mixture was concentrated and purified on a silica gel column to give the product (1 .5 g, 28%). MS ES+ m/z 429 [M+H] ⁺ .

Intermediate example 51

4-Chloro-2-[2-(trifluoromethyl)pyrimidin-5-yl]-1 H-pyrrolo[2,3-b]pyridine

1 M TBAF in THF (20.8 ml, 20.8 mmol) was added to a solution of 2-[[4- chloro-2-[2-(trifluoromethyl)pyrimidin-5-yl]pyrrolo[2,3-b]py ridin-1 - yl]methoxy]ethyl-trimethyl-silane (2 g, 4.16 mmol) in THF (10 ml) and the resulting mixture was refluxed overnight. When cooled to rt the mixture was concentrated, purified on a silica gel column, followed by preparative HPLC to give the product as a solid (530 mg, 43%). MS ES+ m/z 299 [M+H] ⁺ .

Example 52

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[2-(trifluoromethyl)pyrimidin-5-yl]- 1 H-pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 4- chloro-2-[2-(trifluoromethyl)pyrimidin-5-yl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (8 mg, 6%). ¹ H NMR (500 MHz, DMSO- cfe) δ ppm 1 .46 - 1 .60 (m, 1 H), 1 .70 (br s, 2 H), 1 .76 (br d, 2 H), 2.02 (br d, 1 H), 3.07 (br s, 1 H), 4.15 - 4.30 (m, 1 H), 5.50 - 5.68 (m, 1 H), 6.34 (s, 1 H), 6.62 (br s, 1 H), 7.32 (d, 1 H), 7.55 (s, 1 H), 8.42 (d, 1 H), 9.65 (s, 2 H), 10.45 (br s, 1 H), 12.78 (br s, 1 H). MS ES+ m/z 509 [M+H] ⁺ .

Intermediate example 53

2-[[4-Chloro-2-[6-(trifluoromethyl)-3-pyridyl]pyrrolo[2,3-b] pyridin-1 - yl]methoxy]ethyl-trimethyl-silane

The title compound was prepared as described in Intermediate example 50, using 5-bromo-2-(trifluoromethyl)pyridine, to give the product (1 .5 g, 79%). MS ES+ m/z 428 [M+H] ⁺ . Intermediate example 54

4-Chloro-2-[6-(trifluoromethyl)-3-pyridyl]-1 H-pyrrolo[2,3-b]pyridine

The title compound was prepared as described in Intermediate example 51 to give the product (520 mg, 50%). MS ES+ m/z 298 [M+H] ⁺ .

Example 55

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[6-(trifluoromethyl)-3-pyridyl]-1 H- pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 4- chloro-2-[6-(trifluoromethyl)-3-pyridyl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (6 mg, 5%). ¹ H NMR (500 MHz, DMSO- cfe) δ ppm 1 .53 (br d, 1 H), 1 .69 (br s, 2 H), 1 .72 - 1 .86 (m, 2 H), 2.02 (br d, 1 H), 3.07 (br t, 1 H), 4.23 (br d, 1 H), 5.58 (br s, 1 H), 6.35 (s, 1 H), 6.61 (br s, 1 H), 7.30 (d, 1 H), 7.40 (s, 1 H), 8.02 (d, 1 H), 8.39 (d, 1 H), 8.64 (dd, 1 H), 9.40 (d, 1 H), 10.44 (br s, 1 H), 12.68 (br s, 1 H). MS ES+ m/z 508 [M+H] ⁺ . Intermediate example 56

2-[[4-Chloro-2-[5-(trifluoromethyl)-3-pyridyl]pyrrolo[2,3-b] pyridin-1 - yl]methoxy]ethyl-trimethyl-silane

The title compound was prepared as described in Intermediate example 50, using 3-bromo-5-(trifluoromethyl)pyridine, to give the product (800 mg, 30%). MS ES+ m/z 428 [M+H] ⁺ . Intermediate example 57

4-Chloro-2-[5-(trifluoromethyl)-3-pyridyl]-1 H-pyrrolo[2,3-b]pyridine

F

The title compound was prepared as described in Intermediate example 51 to give the product (300 mg, 43%). MS ES+ m/z 298 [M+H] ⁺ .

Example 58

6-[2-(Trifluoromethyl)-1 -piperidyl]-4-[2-[5-(trifluoromethyl)-3-pyridyl]-1 H- pyrrolo[2,3-b]pyridin-4-yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 4- chloro-2-[5-(trifluoromethyl)-3-pyridyl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (7 mg, 6%). ¹ H NMR (500 MHz, DMSO- cfe) δ ppm 1 .47 - 1 .63 (m, 1 H), 1 .69 (br s, 2 H), 1 .72 - 1 .86 (m, 2 H), 2.02 (br d, 1 H), 3.06 (br t, 1 H), 4.24 (br d, 1 H), 5.58 (br s, 1 H), 6.36 (s, 1 H), 6.61 (br s, 1 H), 7.29 (d, 1 H), 7.46 (s, 1 H), 8.38 (d, 1 H), 8.82 (s, 1 H), 8.93 (d, 1 H), 9.52 (d, 1 H), 10.43 (br s, 1 H), 12.63 (br s, 1 H). MS ES+ m/z 508 [M+H] ⁺ .

Intermediate example 59

1 -[(4-Fluorophenyl)methylsulfonyl]-3-(trifluoromethyl)piperaz ine

2-(Trifluoromethyl)-piperazine (2 g, 13 mmol) and TEA (2.17 ml, 15.6 mmol) were dissolved in DCM (30 ml). (4-fluorophenyl)methanesulfonyl chloride (2.71 g, 13 mmol) was added in small portions at 0 °C and the mixture was stirred at rt overnight. Water (45 ml) was added and the mixture extracted with DCM (2 x 80 ml). The combined organics were washed twice with brine, dried over Na2SO ₄ , filtered and concentrated to give the product as a solid (3.5 g, 83%). MS ES+ m/z 327 [M+H] ⁺ . Intermediate example 60

1 -(4-Benzyloxy-6-tert-butoxy-2-pyridyl)-4-[(4- fluorophenyl)methylsulfonyl]-2-(trifluoromethyl)piperazine

A mixture of 1 -[(4-fluorophenyl)methylsulfonyl]-3-(trifluoromethyl)piperaz ine (1 .2 g, 3.68 mmol), 4-benzyloxy-2-tert-butoxy-6-chloro-pyridine (1 .34 g, 4.6 mmol), CS2CO3 (2.4 g, 7.35 mmol), XantPhos (206 mg, 0.37 mmol ) and Pd(OAc)2 (83 mg, 0.37 mmol ) in anh. degassed 1 ,4-dioxane (50 ml) was refluxed overnight under argon. Water was added and the mixture extracted with EtOAc. The combined organics were dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column, eluted with 0-100% EtOAc in Heptane, to give the product (1 .25 g, 58%). MS ES+ m/z 582 [M+H] ⁺ . Intermediate example 61

2-tert-Butoxy-6-[4-[(4-fluorophenyl)methylsulfonyl]-2- (trifluoromethyl)piperazin-1 -yl]pyridin-4-ol

The title compound was prepared as described in Intermediate example 33 to give the product (1 .22 g, 96%). MS ES+ m/z 492 [M+H] ⁺ .

Intermediate example 62

[2-tert-Butoxy-6-[4-[(4-fluorophenyl)methylsulfonyl]-2- (trifluoromethyl)piperazin-l -yl]-4-pyridyl] trifluoromethanesulfonate

The title compound was prepared as described in Intermediate example 34 to give the product (700 mg, 45%). MS ES+ m/z 624 [M+H] ⁺ . Intermediate example 63

1 -[6-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2- pyridyl]-4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluorometh yl)piperazine

H ₃ C C H ₃

The title compound was prepared as described in Intermediate example 35 to give the product (490 mg, 73%). MS ES+ m/z 602 [M+H] ⁺ . Intermediate example 64

4-[2-tert-Butoxy-6-[4-[(4-fluorophenyl)methylsulfonyl]-2- (trifluoromethyl)piperazin-1 -yl]-4-pyridyl]-1 H-pyrrolo[2,3-b]pyridine

The title compound was prepared as described in Intermediate example 3, starting from 1 -[6-tert-butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)- 2-pyridyl]-4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluorome thyl)piperazine, to give the product (70 mg, 49%). MS ES+ m/z 592 [M+H] ⁺ .

Example 65

6-[4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluoromethyl) piperazin-1 -yl]-4- (1 H-pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 4, using 4-[2-tert- Butoxy-6-[4-[(4-fluorophenyl)methylsulfonyl]-2-(trifluoromet hyl)piperazin-1 -yl]- 4-pyridyl]-1 H-pyrrolo[2,3-b]pyridine, to give the product (15 mg, 24%). ¹ H NMR (500 MHz, DMSO-cfe) δ ppm 2.85 - 3.00 (m, 1 H), 3.10 - 3.28 (m, 2 H), 3.58 - 3.66 (m, 1 H), 3.86 - 3.92 (m, 1 H), 4.28 - 4.35 (m, 1 H), 4.47 - 4.56 (m, 1 H), 4.50 - 4.52 (m, 1 H), 5.56 (br s, 1 H), 6.33 - 6.40 (m, 1 H), 6.54 - 6.60 (m, 1 H), 6.61 - 6.66 (m, 1 H), 7.16 - 7.26 (m, 3 H), 7.45 - 7.51 (m, 2 H), 7.55 - 7.61 (m, 1 H), 8.27 - 8.32 (m, 1 H), 10.61 (br s, 1 H), 1 1 .85 (br s, 1 H). MS ES+ m/z 536 [M+H] ⁺ .

Intermediate example 66

1 -Ethylsulfonyl-3-(trifluoromethyl)piperazine

The title compound was prepared as described in Intermediate example 59, using ethanesulfonyl chloride, to give the product as a solid (3 g, 98%). MS ES+ m/z 247 [M+H] ⁺ .

Intermediate example 67

1 -(4-Benzyloxy-6-tert-butoxy-2-pyridyl)-4-ethylsulfonyl-2- (trifluoromethyl)piperazine

The title compound was prepared as described in Intermediare example 60, using 1 -ethylsulfonyl-3-(trifluoromethyl)piperazine, to give the product as a solid (2.53 g, 67%). MS ES+ m/z 502 [M+H] ⁺ .

Intermediate example 68

2-tert-Butoxy-6-[4-ethylsulfonyl-2-(trifluoromethyl)piperazi n-1 -yl]pyridin- 4-ol

The title compound was prepared as described in Intermediate example 33 to give the product (1 .94 g, 85%). MS ES+ m/z 412 [M+H] ⁺ .

Intermediate example 69

[2-tert-Butoxy-6-[4-ethylsulfonyl-2-(trifluoromethyl)piperaz in-1 -yl]-4- pyridyl] trifluoromethanesulfonate

The title compound was prepared as described in Intermediate example 34 to give the product (1 .56 g, 62%). MS ES+ m/z 544 [M+H] ⁺ .

Intermediate example 70

1 -[6-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2- pyridyl]-4-ethylsulfonyl-2-(trifluoromethyl)piperazine

The title compound was prepared as described in Intermediate example 35 to give the product (1 .2 g, 86%). MS ES+ m/z 440 [M+H] ⁺ (boronic acid).

Example 71

6-[4-Ethylsulfonyl-2-(trifluoromethyl)piperazin-1 -yl]-4-(1 H-pyrrolo[2,3- b]pyridin-4-yl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 1 -[6-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-4- ethylsulfonyl-2-(trifluoromethyl)piperazine and 4-chloro-1 H-pyrrolo[2,3- b]pyridine, to give the product (6 mg, 7%). ¹ H NMR (500 MHz, DMSO-c/e) δ ppm 1 .23 (t, 3 H), 2.97 - 3.05 (m, 1 H), 3.13 (q, 2 H), 3.22 - 3.30 (m, 2 H), 3.66 (br d, 1 H), 3.95 (br d, 1 H), 4.34 (br d, 1 H), 5.62 (br s, 1 H), 6.37 (s, 1 H), 6.59 (dd, 1 H), 6.65 (br s, 1 H), 7.24 (d, 1 H), 7.58 (s, 1 H), 8.30 (d, 1 H), 10.58 (br s, 1 H), 1 1 .85 (br s, 1 H). MS ES+ m/z 456 [M+H] ⁺ .

Example 72

4-(2-Cyclopropyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[4-ethylsulfonyl-2- (trifluoromethyl)piperazin-l -yl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 1 -[6-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-4- ethylsulfonyl-2-(trifluoromethyl)piperazine and 4-chloro-2-cyclopropyl-1 H- pyrrolo[2,3-b]pyridine, to give the product (6 mg, 6%). ¹ H NMR (500 MHz, METHANOL-c/4) δ ppm 0.84 - 0.94 (m, 2 H), 1 .04 - 1 .09 (m, 2 H), 1 .36 (t, 3 H), 2.07 - 2.13 (m, 1 H), 3.03 - 3.16 (m, 3 H), 3.29 (br s, 1 H), 3.45 (br t, 1 H), 3.80 (br d, 1 H), 4.10 - 4.21 (m, 2 H), 5.57 (br s, 1 H), 6.28 (s, 1 H), 6.40 (s, H), 6.55 (s, 1 H), 7.17 (d, 1 H), 8.14 (d, 1 H). MS ES+ m/z 496 [M+H] ⁺ .

Example 73

6-[4-Ethylsulfonyl-2-(trifluoromethyl)piperazin-1 -yl]-4-(2-methyl-1 H- pyrrolo[2,3-b]pyridin-4-yl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 1 -[6-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-4- ethylsulfonyl-2-(trifluoromethyl)piperazine and 4-chloro-2-methyl-1 H- pyrrolo[2,3-b]pyridine, to give the product as a solid (10 mg, 1 1 %). ¹ H NMR (500 MHz, DMSO-c/e) δ ppm 1 .20 - 1 .29 (m, 3 H), 2.43 (s, 3 H), 2.97 - 3.03 (m, 1 H), 3.09 - 3.18 (m, 2 H), 3.21 - 3.30 (m, 2 H), 3.58 - 3.79 (m, 1 H), 3.95 (br d, 1 H), 4.32 (br d, 1 H), 5.55 - 5.76 (m, 1 H), 6.23 - 6.42 (m, 2 H), 6.63 (br s, 1 H), 7.17 (d, 1 H), 8.17 (d, 1 H), 10.54 (br s, 1 H), 1 1 .67 (s, 1 H). MS ES+ m/z 470 [M+H] ⁺ .

Example 74

4-[2-[4-Ethylsulfonyl-2-(trifluoromethyl)piperazin-1 -yl]-6-oxo-1 H-pyridin- 4-yl]-1 H-pyrrolo[2,3-b]pyridine-2-carbonitrile

The title compound was prepared as described in Example 36, using 1 -[6-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-pyridyl]-4- ethylsulfonyl-2-(trifluoromethyl)piperazine and 4-chloro-1 H-pyrrolo[2,3- b]pyridine-2-carbonitrile, to give the product as a solid (6 mg, 6%). ¹ H NMR (500 MHz, METHANOL-^) δ ppm 1 .36 (t, 3 H), 3.1 1 (d, 3 H), 3.27 - 3.32 (m, 1 H), 3.42 - 3.50 (m, 1 H), 3.72 - 3.89 (m, 1 H), 4.10 - 4.17 (m, 1 H), 4.17 - 4.25 (m, 1 H), 5.64 (br d, 1 H), 6.39 (d, 1 H), 6.57 (s, 1 H), 7.36 - 7.42 (m, 2 H), 8.51 - 8.56 (m, 1 H). MS ES+ m/z 481 [M+H] ⁺ .

Intermediate example 75

4-Benzyloxy-2-tert-butoxy-6-[2-(trifluoromethyl)phenyl]pyrid ine

4-Benzyloxy-2-tert-butoxy-6-chloro-pyridine (1 .46 g, 5 mmol), [2- (trifluoromethyl)phenyl]boronic acid (950 mg, 5 mmol), K2CO3 (1 .73 g, 12.5 mmol) and PdCl2(dppf) (366 mg, 0.5 mmol) were dissolved in 1 ,4-dioxane (25 ml) and water (5 ml) and the resulting mixture was stirred at 90 °C for 2 h. When cooled to rt the mixture was diluted with water and EtOAc. The organic layer was separated and the aqueous layer extracted with EtOAc. The combined organics were filtered through celite, dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-80% EtOAc in heptane to give the product as a solid (1 .58 g, 79%). MS ES+ m/z 402

[M+H] ⁺ .

Intermediate example 76

2-tert-Butoxy-6-[2-(trifluoromethyl)phenyl]pyridin-4-ol

The title compound was prepared as described in Intermediate example 33 to give the product (948 mg, 72%). MS ES+ m/z 312 [M+H] ⁺ . Intermediate example 77

[2-tert-Butoxy-6-[2-(trifluoromethyl)phenyl]-4-pyridyl]

trifluoromethanesulfonate

The title compound was prepared as described in Intermediate example 34 to give the product (720 mg, 54%). MS ES+ m/z 388 [M-tBu] ⁺ .

Intermediate example 78

2-tert-Butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2- (trifluoromethyl)phenyl]pyridine

H _β C C H 2

The title compound was prepared as described in Intermediate example 35 to give the product (450 mg, 76%). MS ES+ m/z 340 [M+H] ⁺ (boronic acid). Example 79

4-(2-cyclopropyl-1 H-pyrrolo[2,3-b]pyridin-4-yl)-6-[2- (trifluoromethyl)phenyl]-1 H-pyridin-2-one

The title compound was prepared as described in Example 36, using 2-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2-

(trifluoromethyl)phenyl]pyridine and 4-chloro-2-cyclopropyl-1 H-pyrrolo[2,3- b]pyridine, to give the product as a solid (10 mg, 10%). ¹ H NMR (500 MHz, DMSO-c/e) δ ppm 0.84 - 0.91 (m, 2 H), 0.98 - 1 .05 (m, 2 H), 2.04 - 2.12 (m, 1 H), 6.23 - 6.29 (m, 1 H), 6.44 - 6.65 (m, 1 H), 6.69 - 6.78 (m, 1 H), 7.12 - 7.17 (m, 1 H), 7.66 - 7.70 (m, 1 H), 7.71 - 7.76 (m, 1 H), 7.78 - 7.83 (m, 1 H), 7.88 - 7.92 (m, 1 H), 8.13 - 8.19 (m, 1 H), 1 1 .74 (br s, 1 H), 1 1 .66 - 1 1 .79 (m, 1 H). MS ES+ m/z 396 [M+H] ⁺ . Example 80

4-[2-Oxo-6-[2-(trifluoromethyl)phenyl]-1 H-pyridin-4-yl]-1 H-pyrrolo[2,3- b]pyridine-2-carbonitrile

The title compound was prepared as described in Example 36, using 2-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2- (trifluoromethyl)phenyl]pyridine and 4-chloro-1 H-pyrrolo[2,3-b]pyridine-2- carbonitrile and purifying the intermediate on a short silica gel column before addition of TFA, to give the product as a solid (5 mg, 3%). ¹ H NMR (500 MHz, DMSO-c/e) δ ppm 6.44 - 6.69 (m, 1 H), 6.74 - 6.82 (m, 1 H), 7.38 - 7.44 (m, 1 H), 7.46 - 7.52 (m, 1 H), 7.68 - 7.76 (m, 2 H), 7.76 - 7.83 (m, 1 H), 7.86 - 7.91 (m, 1 H), 8.54 - 8.59 (m, 1 H), 1 1 .34 - 12.51 (m, 1 H), 12.64 - 13.76 (m, 1 H). MS ES+ m/z 381 [M+H] ⁺ . Intermediate example 81

4-[2-tert-Butoxy-6-[2-(trifluoromethyl)phenyl]-4-pyridyl]-1 H-pyrazolo[3,4- b]pyridine

A mixture of 4-chloro-1 H-pyrazolo[3,4-b]pyridine (61 mg, 0.4 mmol), 2-tert- butoxy-4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-6-[2-

(trifluoromethyl)phenyl]pyridine (21 1 mg, 0.5 mmol), K2CO3 (1 1 1 mg, 0.8 mmol) and PdCl2(Amphos) (15 mg, 0.02 mmol) in 1 ,4-dioxane (3 ml) and water (0.75 ml) was stirred at 90 °C for 2.5 h. When cooled to rt brine was added and the mixture extracted with EtOAc. The combined organics were dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-50% EtOAc in heptane to give the product as a solid (52 mg, 32%). MS ES+ m/z 413 [M+H] ⁺ .

Example 82

4-(1 H-pyrazolo[3,4-b]pyridin-4-yl)-6-[2-(trifluoromethyl)phenyl] -1 H- pyridin-2-one

TFA (0.66 ml, 8.83 mmol) was added to a solution of 4-[2-tert-butoxy-6-[2- (trifluoromethyl)phenyl]-4-pyridyl]-1 H-pyrazolo[3,4-b]pyridine (52.0 mg, 0.13 mmol) in DCM (6 ml) at 0 °C and the resulting mixture was stirred at rt for 1 h. The mixture was concentrated, chased with toluene and purified by

preparative HPLC to give the product as a solid (28 mg, 62%). ¹ H NMR (500 MHz, DMSO-c/e) δ ppm 6.67 (br s, 1 H), 6.87 (br s, 1 H), 7.43 (d, 1 H), 7.69 - 7.77 (m, 2 H), 7.79 - 7.83 (m, 1 H), 7.90 (d, 1 H), 8.27 (s, 1 H), 8.62 (d, 1 H), 1 1 .61 - 12.46 (m, 1 H), 13.95 (br s, 1 H). MS ES+ m/z 357 [M+H] ⁺ .

Intermediate example 83

1 -Methylsulfonyl-3-(trifluoromethyl)piperazine

The title compound was prepared as described in Intermediate example 59, using methanesulfonyl chloride, to give the product as a solid (2.2 g, 97%). ¹ H NMR (500 MHz, DMSO-cfe) δ ppm 2.66 - 2.74 (m, 1 H), 2.76 - 2.85 (m, 2 H), 2.93 (s, 3 H), 2.96 - 3.02 (m, 2 H), 3.28 - 3.34 (m, 2 H), 3.45 - 3.52 (m, 2 H).

Intermediate example 84

1 -(4-Benzyloxy-6-tert-butoxy-2-pyridyl)-4-methylsulfonyl-2- (trifluoromethyl)piperazine

The title compound was prepared as described in Intermediate example 60, using 1 -methylsulfonyl-3-(trifluoromethyl)piperazine, to give the product as a solid (900 mg, 33%). MS ES+ m/z 488 [M+H] ⁺ .

Intermediate example 85

2-tert-Butoxy-6-[4-methylsulfonyl-2-(trifluoromethyl)pipe razin-1 - yl]pyridin-4-ol

The title compound was prepared as described in Intermediate example 33, with DMF as co-solvent, to give the product (750 mg, 92%). MS ES+ m/z 398 [M+H] ⁺ . Intermediate example 86

[2-tert-Butoxy-6-[4-methylsulfonyl-2-(trifluoromethyl)pipera zin-1 -yl]-4- p ridyl] trifluoromethanesulfonate

The title compound was prepared as described in Intermediate example 34, replacing trifluoromethylsulfonyl trifluoromethanesulfonate for 1 ,1 ,1 -trifluoro- N-phenyl-N-(trifluoromethylsulfonyl)methanesulfonamide and stirring the mixture at rt overnight, to give the product as a solid (680 mg, 69%). MS ES+ m/z 530 [M+H] ⁺ .

Intermediate example 87

4-[2-tert-Butoxy-6-[4-methylsulfonyl-2-(trifluoromethyl)pipe razin-1 -yl]-4- pyridyl]-1 H-pyrazolo[3,4-b]pyridine

A mixture of [2-tert-butoxy-6-[4-methylsulfonyl-2-(trifluoromethyl)pipera zin-1 - yl]-4-pyridyl] trifluoromethanesulfonate (265 mg, 0.5 mmol), 4,4,5,5- tetramethyl-2-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 ,3,2- dioxaborolane (152 mg, 0.6 mmol), KOAc (98 mg, 1 mmol) and PdCl2(dppf) (37 mg, 0.05 mmol) in 1 ,4-dioxane (4 ml) under an argon atmosphere was heated and stirred at 95 °C overnight. When cooled to rt, 4-bromo-1 H- pyrazolo[3,4-b]pyridine (99 mg, 0.5 mmol), K2CO3 (138 mg, 1 mmol),

PdCl2(dppf) (18 mg, 0.03 mmol) and water (1 ml) were added and the resulting mixture was heated and stirred at 95 °C for 3 h. When cooled to rt, water and brine were added, and the mixture was extracted with EtOAc. The combined organics were washed with brine, dried over Na2SO ₄ , filtered, concentrated and purified on a silica gel column eluted with 0-6% MeOH in DCM to give the product as a solid (210 mg, 84%). MS ES+ m/z 499 [M+H] ⁺ .

Example 88

6-[4-Methylsulfonyl-2-(trifluoromethyl)piperazin-1 -yl]-4-(1 H-pyrazolo[3,4- b]pyridin-4-yl)-1 H-pyridin-2-one

The title compound was prepared as described in Example 82, to give the product as a solid (65 mg, 37%). ¹ H NMR (500 MHz, DMSO-c/ ₆ ) δ ppm 2.90 - 2.96 (m, 4 H), 3.15 - 3.24 (m, 1 H), 3.31 - 3.45 (m, 2 H), 3.62 (br d, 1 H), 3.89 (br d, 1 H), 4.44 (br d, 1 H), 5.70 (br s, 1 H), 6.44 (d, 1 H), 6.76 (s, 1 H), 7.43 (d, 1 H), 8.29 (s, 1 H), 8.62 (d, 1 H), 10.56 - 1 1 .30 (m, 1 H). MS ES+ m/z 443 [M+H] ⁺ .

Example 89

Vps34 biochemical assay

Dilution series of compounds of the invention were prepared in DMSO at 100 times the final assay concentration (ni =no/3 in 10 points). The compounds were further diluted to 4 times the assay concentration in assay buffer (Life technologies buffer Q, PV5125, diluted 5 times supplemented with 2 mM DTT and 2 mM MnC ). 2.5 μΙ of the diluted compounds were added to a 384 well assay plate followed by 2.5 μΙ of 16.5 nM Vps34 enzyme (Life technologies, PV5126). Enzyme and compounds were pre-incubated at rt for 15 min. Then 5 μΙ of substrate mix containing 20 μΜ ATP (Life technologies, PV3227) and 200 μΜ PIPS substrate (Life technologies, PV5122) in assay buffer was added to the wells containing compound and enzyme. Mixing was performed by pipetting several times. The reaction was incubated at room temperature for 1 h. Then 5 μΙ stop-detection mix, prepared as described in the Adapta kinase assay kit instructions (Life technologies, PV5099) containing Adapta Eu-anti-ADP antibody (2.3 nM), Alexa Fluor 647 ADP tracer (9 nM) and EDTA (30 mM) in TR-FRET buffer, was added to quench the reaction. Mixing was performed by pipetting several times. The assay plate was then incubated at room temperature for 30 min and read with Artemis micro plate reader.

Percent inhibition of the compounds as compared to DMSO treated control samples was calculated. By the use of Dotmatics software compound concentration versus percent inhibition was fitted to generate IC50 values. The example compounds effectively inhibited Vps34 and the results of the assay are shown in Table 1 (Median IC50 nM Adapta).

Table 1. Median IC ₅₀ values for the Vps34 assay

Example Compound Median IC50 nM Adapta

4 <5

8 10

6 20

16 12

12 24

23 8

27 <5

29 <5

37 <5

36 <5

45 34

48 <5

49 <5

52 <5

42 <5

55 <5

58 7

72 <5

71 <5

65 <5

73 <5

74 <5

79 <5

80 <5

82 <5

88 <5 Example 90

High Content Screening Autophagy assay

Human osteosarcoma cells (HOS) stably expressing a Green Fluorescent Protein (GFP) tagged LC3 (GFP-LC3) were used to determine the inhibitory effect on autophagy of proprietary compounds. For that purpose, autophagy was activated by using the mTOR inhibitor KU-0063794 at 500 nM in the presence of Bafilomycin A1 (Sigma-Aldrich) at 5 nM. Shortly, cells were plated overnight in clear bottom 96-well plates in DMEM-High Modified media (Hi-Clone Cat # SH30285.01 ). At the start of the experiment, the media was removed and replaced with fresh media containing the mTOR inhibitor,

Bafilomycin A1 and the vehicle or a test compound as indicated. After 6 hours the media was removed, cells were washed twice with ice-cold phosphate buffered saline (PBS) and fixed with 4% paraformaldehyde for 20 minutes at room temperature. Then the cells were washed twice with ice-cold PBS before adding Hoechst 33342 at 1 g/ml in PBS for nuclear staining. After incubation overnight at 4°C, cells were washed once with PBS to remove the excess of dye and 100 μΙ of PBS was added to each well. Images were acquired at 20x magnification, 6 images per well, using the ImageXpress automated microscope (Molecular Devices Inc.) and analyzed with

MetaXpress software to identify LC3-GFP foci. Foci area per cell values were used to generate dose response curves and IC50 values were calculated using the non-linear fitting analysis in GraphPad Prism software.

The tested example compounds effectively inhibited autophagy in HOS cells. The results of the assay are shown in Table 2 (Median IC50 nM HOS-LC3).

Table 2. Median IC ₅₀ values for the Vps34 assay and autophagy in HOS cells assay.

Example Median IC50 (nM)

Compound Cellular assay

4 665

8 366

6 2567

12 554

23 44

27 16

29 54

37 145

36 62

49 62

42 473

72 19

71 308

65 176

74 89

73 28