Title:
B DOMAIN AND Z DOMAIN MUTANTS OF PROTEIN A, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/143525
Kind Code:
A1
Abstract:
B domain and Z domain mutants of a protein A, and an application thereof. Specifically, provided is an isolated polypeptide, which is selected from: (1) a polypeptide having a substitution mutation at one or more positions selected from positions 3, 6, 9, 15 and 23 compared with the native B domain of protein A shown in SEQ ID NO: 1 or the Z domain of protein A shown in SEQ ID NO: 2; and (2) a polypeptide having at least 85% sequence identity with the polypeptide described in (1) and retaining the substitution mutation at one or more of the positions 3, 6, 9, 15 and 23. Also provided are a fusion protein or protein A comprising the polypeptide, and a separation matrix. The fusion protein or protein A comprising the polypeptide has significantly improved alkaline stability during alkaline cleaning.
Inventors:
ZHANG HONG (CN)
SHI HAITAO (CN)
ZHANG YAN (CN)
HU HUIXIA (CN)
SHI HAITAO (CN)
ZHANG YAN (CN)
HU HUIXIA (CN)
Application Number:
PCT/CN2023/073587
Publication Date:
August 03, 2023
Filing Date:
January 28, 2023
Export Citation:
Assignee:
BESTCHROM ZHEJIANG BIOSCIENCES LTD (CN)
BESTCHROM SHANGHAI BIOSCIENCES LTD (CN)
BESTCHROM SHANGHAI BIOSCIENCES LTD (CN)
International Classes:
C07K14/31; B01D15/38; C07K1/22; C07K16/00; C07K19/00; C12N15/31; C12N15/62
Foreign References:
| CN114409748A | 2022-04-29 | |||
| CN105377880A | 2016-03-02 | |||
| CN102516371A | 2012-06-27 | |||
| US20160159855A1 | 2016-06-09 | |||
| US20150080554A1 | 2015-03-19 | |||
| US20190177376A1 | 2019-06-13 |
Other References:
ZHANG, ZHONGHAO ET AL.: "Extraction, Purification and Identification of Staphylococcal Protein A", HEILONGJIANG ANIMAL SCIENCE AND VETERINARY MEDICINE, no. 6, 20 March 2018 (2018-03-20), CN , pages 83 - 85, XP009548110, ISSN: 1004-7034, DOI: 10.13881/j.cnki.hljxmsy.2017.04.0129
HOBER, S. NORD, K. LINHULT, M.: "Protein A chromatography for antibody purification", JOURNAL OF CHROMATOGRAPHY B, ELSEVIER, AMSTERDAM., NL, vol. 848, no. 1, 12 March 2007 (2007-03-12), NL , pages 40 - 47, XP005922826, ISSN: 1570-0232, DOI: 10.1016/j.jchromb.2006.09.030
HOBER, S. NORD, K. LINHULT, M.: "Protein A chromatography for antibody purification", JOURNAL OF CHROMATOGRAPHY B, ELSEVIER, AMSTERDAM., NL, vol. 848, no. 1, 12 March 2007 (2007-03-12), NL , pages 40 - 47, XP005922826, ISSN: 1570-0232, DOI: 10.1016/j.jchromb.2006.09.030
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE , LLC (CN)
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