Title:
A BACILLUS PUMILUS STRAIN FOR HIGH yield OF Tetramethylpyrazine
Document Type and Number:
WIPO Patent Application WO/2007/137510
Kind Code:
A1
Abstract:
A bacillus pumilus strain for high yield of tetramethylpyrazine, named as Bacillus pumilus RX3-17, deposited with China Center for Type Culture Collection under the deposit number CCTCC M 206043 on 19 Apr. 2006. The strain, isolated from soil, is rod-shaped with the dimension of 0.6-0.7μm, and the color of its colony is milk white. The strain has typical physiological and biochemical characteristics of bacillus pumilus. The 16S rDNA sequence of the strain has 99% similarity with the 16S rDNA sequences of other bacillus pumilus strains. The invention belongs to the biotechnology field. The strain can be used to produce tetramethylpyrazine from glucose, solving the problem of low concentration of the fermentation products in the method of producing tetramethylpyrazine by microorganism fermentation.
More Like This:
Inventors:
XU PING (CN)
XIAO ZIJUN (CN)
WEI ZHONGHAO (CN)
DU YI (CN)
XIAO ZIJUN (CN)
WEI ZHONGHAO (CN)
DU YI (CN)
Application Number:
PCT/CN2007/001705
Publication Date:
December 06, 2007
Filing Date:
May 25, 2007
Export Citation:
Assignee:
SHANGHAI APPLE FLAVOR & FRAGRA (CN)
SHANGHAI KAIXIN BIOTECH CO LTD (CN)
XU PING (CN)
XIAO ZIJUN (CN)
WEI ZHONGHAO (CN)
DU YI (CN)
SHANGHAI KAIXIN BIOTECH CO LTD (CN)
XU PING (CN)
XIAO ZIJUN (CN)
WEI ZHONGHAO (CN)
DU YI (CN)
International Classes:
C12N1/20; C12N1/04; C12P17/12; C12R1/07
Foreign References:
| KR940005652B1 | 1994-06-22 | |||
| FR2728913A1 | 1996-07-05 |
Other References:
XIAO Z.J. ET AL.: "Tetramethylpyrazine production from glucose by a newly isolated Bacillus mutant", APPL. MICROBIOL. BIOTECHNOL., vol. 73, no. 3, 31 December 2006 (2006-12-31), pages 514 - 515, XP019458722
LARROCHE C. ET AL.: "High pyrazine production by Bacillus subtilis in solid substrate fermentation on ground soybeans", PROCESS BIOCHEMISTRY, vol. 34, no. 6-7, 31 December 1999 (1999-12-31), pages 667 - 674, XP008090988
DEMYTTENAERE J. ET AL.: "Production of pyrazines and 2-acetyl-1-pyrroline by Bacillus cereus strains", FLAVOUR RESEARCH AT THE DAWN OF THE TWENTY-FIRST CENTURY, 31 December 2003 (2003-12-31), pages 344 - 349, XP008090497
LARROCHE C. ET AL.: "High pyrazine production by Bacillus subtilis in solid substrate fermentation on ground soybeans", PROCESS BIOCHEMISTRY, vol. 34, no. 6-7, 31 December 1999 (1999-12-31), pages 667 - 674, XP008090988
DEMYTTENAERE J. ET AL.: "Production of pyrazines and 2-acetyl-1-pyrroline by Bacillus cereus strains", FLAVOUR RESEARCH AT THE DAWN OF THE TWENTY-FIRST CENTURY, 31 December 2003 (2003-12-31), pages 344 - 349, XP008090497
Attorney, Agent or Firm:
WATSON & BAND LAW OFFICES (26th/F WenXin United Press Tower,755 WeiHai Road, Shanghai 1, CN)
Download PDF: