Title:
BACTERIAL CULTURE
Document Type and Number:
WIPO Patent Application WO/2000/063343
Kind Code:
A2
Abstract:
The present invention provides a pure bacterial culture isolable from a mixed bacterial culture ATCC No. 202057 and capable of degrading methyl t-butyl ether (MTBE) to carbon dioxide; a process for isolating such a pure bacterial culture, and a process for remediating a medium containing water and a branched alkyl ether (e.g. methyl t-butyl ether) and/or a branched alkyl alcohol (e.g. t-butyl alcohol (TBA)) using said pure bacterial culture.
Inventors:
SALANITRO JOSEPH PATRICK
Application Number:
PCT/EP2000/003254
Publication Date:
October 26, 2000
Filing Date:
April 10, 2000
Export Citation:
Assignee:
SHELL INT RESEARCH (NL)
International Classes:
A62D3/02; B09C1/10; C12N1/20; (IPC1-7): C12N1/00
Foreign References:
| DE19962722A1 | 2000-06-29 | |||
| FR2787783A1 | 2000-06-30 | |||
| US5811010A | 1998-09-22 | |||
| US5750364A | 1998-05-12 | |||
| US5698441A | 1997-12-16 |
Other References:
TIMMIS K N ET AL: "Bacteria designed for bioremediation" TRENDS IN BIOTECHNOLOGY,NL,ELSEVIER, AMSTERDAM, vol. 17, no. 5, May 1999 (1999-05), pages 200-204, XP004181358 ISSN: 0167-7799
FEAKIN S J ET AL: "Inoculation of granular activated carbon in a fixed bed with s-triazine-degrading bacteria as a water treatment process" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 3, 1 March 1995 (1995-03-01), pages 819-825, XP004035425 ISSN: 0043-1354
"BIOTREATING PRODUCED WATER COULD SAVE COSTS, BENEFIT ENVIRONMENT" OIL AND GAS JOURNAL,US,PENNWELL PUBLISHING CO. TULSA, vol. 94, no. 19, 6 May 1996 (1996-05-06), page 111 XP000595938 ISSN: 0030-1388
PREVOST M ET AL: "Suspended bacterial biomass and activity in full-scale drinking water distribution systems: Impact of water treatment" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 32, no. 5, 1 March 1998 (1998-03-01), pages 1393-1406, XP004130951 ISSN: 0043-1354
FEAKIN S J ET AL: "Inoculation of granular activated carbon with s-triazine-degrading bacteria for water treatment at pilot-scale" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 7, 1 July 1995 (1995-07-01), pages 1681-1688, XP004035254 ISSN: 0043-1354
FEAKIN S J ET AL: "Inoculation of granular activated carbon in a fixed bed with s-triazine-degrading bacteria as a water treatment process" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 3, 1 March 1995 (1995-03-01), pages 819-825, XP004035425 ISSN: 0043-1354
"BIOTREATING PRODUCED WATER COULD SAVE COSTS, BENEFIT ENVIRONMENT" OIL AND GAS JOURNAL,US,PENNWELL PUBLISHING CO. TULSA, vol. 94, no. 19, 6 May 1996 (1996-05-06), page 111 XP000595938 ISSN: 0030-1388
PREVOST M ET AL: "Suspended bacterial biomass and activity in full-scale drinking water distribution systems: Impact of water treatment" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 32, no. 5, 1 March 1998 (1998-03-01), pages 1393-1406, XP004130951 ISSN: 0043-1354
FEAKIN S J ET AL: "Inoculation of granular activated carbon with s-triazine-degrading bacteria for water treatment at pilot-scale" WATER RESEARCH,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 7, 1 July 1995 (1995-07-01), pages 1681-1688, XP004035254 ISSN: 0043-1354
Download PDF:
Claims:
C L A I M S
| 1. | A pure bacterial culture isolable from a mixed bacterial culture ATCC No. 202057 and capable of degrading methyl tbutyl ether (MTBE) to carbon dioxide. |
| 2. | A pure bacterial culture according to claim 1 which pure culture is an aerobic, gram stain positive culture. |
| 3. | A pure bacterial culture according to claim 1 or claim 2, belonging to the genus Rhodococcus. |
| 4. | A pure bacterial culture according to any one of claims 13, having a specific activity of from 0.1 to 100 mg MTBE/g cells/hour at 9°C. |
| 5. | A pure bacterial culture according to any one of claims 14, which culture is capable of aerobically degrading at least 10% of the methyl tbutyl ether (MTBE) and/or tbutyl alcohol (TBA) added to the culture at a concentration in the range 0.01 to 500 ppm, to carbon dioxide within 70 hours. |
| 6. | A process for isolating a pure bacterial culture according to any one of claims 15, which process comprises a) adding sterile mineral dilution medium containing 0.011000 mg/L of MTBE, to a sample of mixed bacterial culture ATCC No. 202057, b) subsequently removing a proportion from the resulting culture volume and replacing that proportion with a corresponding volume of fresh dilution medium containing 0.011000 mg/L of MTBE, to yield a dilution enrichment culture, c) repeating step b) on resulting culture until a dilute suspension of dilution enrichment culture is found to consistently degrade MTBE, d) streaking the dilution enriched culture of step c) onto sterile plates containing a mineral medium and incubating said plates, e) observing the plates for the appearance of colonies and when colonies appear, picking the colonies from the plates and inoculating the separated colonies in sealed vessels containing mineral medium and MTBE, f) incubating the separated colonies and determining the loss of MTBE from headspace of the sealed vessels, and g) selecting and isolating a separated colony which substantially completely degrades MTBE to carbon dioxide. |
| 7. | A process according to claim 6, which further comprises h) growing the isolated pure culture in a sugar containing mineral solution and inducing enhanced MTBE and/or TBA degradation activity by adding MTBE and/or TBA to the resulting culture. |
| 8. | A process for remediating a medium containing water and a branched alkyl ether and/or a branched alkyl alcohol, which process comprises growing in the presence of said medium under aerobic conditions a pure bacterial culture according to any one of claims 15, or a pure bacterial culture prepared by a process according to claim 6 or 7. |
| 9. | A process according to claim 8, wherein the branched alkyl ether and/or a branched alkyl alcohol is present in the medium at a concentration in the range 0.001 to 5000 ppmw. |
| 10. | A process according to claim 8 or claim 9, wherein the medium contains MTBE and/or TBA. |
Description:
INTERNATIONAL SEARCH REPORT Intern. ial Application No PCT/EP 00/03254 C. (Continuation) DOCUMENTS CONSIDERED TO BE RELEVANT Category ° Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. A US 5 698 441 A (VARADARAJ RAMESH) 1-9 16 December 1997 (1997-12-16) the whole document A, P TIMMIS K N ET AL:"Bacteria designed for bioremediation" TRENDS IN BIOTECHNOLOGY, NL, ELSEVIER, AMSTERDAM, vol. 17, no. 5, May 1999 (1999-05), pages 200-204, XP004181358 ISSN: 0167-7799 the whole document A FEAKIN S J ET AL:"Inoculation of granular activated carbon in a fixed bed with s-triazine-degrading bacteria as a water treatment process" WATER RESEARCH, NL, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 3, 1 March 1995 (1995-03-01), pages 819-825, XP004035425 ISSN: 0043-1354 abstract A"BIOTREATING PRODUCED WATER COULD SAVE COSTS, BENEFIT ENVIRONMENT" OIL AND GAS JOURNAL, US, PENNWELL PUBLISHING CO. TULSA, vol. 94, no. 19,6 May 1996 (1996-05-06), page 111 XP000595938 ISSN: 0030-1388 the whole document A PREVOST M ET AL:"Suspended bacterial biomass and activity in full-scale drinking water distribution systems: Impact of water treatment" WATER RESEARCH, NL, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 32, no. 5, 1 March 1998 (1998-03-01), pages 1393-1406, XP004130951 ISSN: 0043-1354 the whole document A FEAKIN S J ET AL :"Inoculation of granular activated carbon with s-triazine-degrading bacteria for water treatment at pilot-scale" WATER RESEARCH, NL, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 29, no. 7,1 July 1995 (1995-07-01), pages 1681-1688, XP004035254 ISSN: 0043-1354 abstract 1 INTERNATIONAL SEARCH REPORT Intern, ial Applieation No Informatlon on patent family members PCT/EP 00/03254 Patent document Publication Patent family Publication cited in search report date member (s) date DE 19962722 A 29-06-2000 FR 2787783 A 30-06-2000 NL 1013882 A 30-06-2000 FR 2787783 A 30-06-2000 DE 19962722 A 29-06-2000 NL 1013882 A 30-06-2000 US 5811010 A 22-09-1998 NONE US 5750364 A 12-05-1998 US 5902734 A 11-05-1999 US 5698441 A 16-12-1997 NONE US 5698441 A 16-12-1997 NONE
Previous Patent: METHOD AND COMPOSITION FOR REDUCED WATER DAMAGE LAUNDRY CARE
Next Patent: METHODS FOR ENHANCING PLANT DEFENSE
Next Patent: METHODS FOR ENHANCING PLANT DEFENSE