Title:
BACTERICIDAL NITROGEN-FIXING FLUORESCENT PSEUDOMONAS PROTEGENS, FERMENTATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/080638
Kind Code:
A1
Abstract:
Provided is a strain of bactericidal nitrogen-fixing fluorescent Pseudomonas protegens CHA0-ΔretS-NiF, a fermentation method therefor and an application thereof. The strain is kept under the accession number CGMCC No. 14476, the optimum culture condition is pH 7, temperature of 28°C, and rotation speed of 600 rpm. Further provided is a microbial agent containing the fluorescent Pseudomonas protegens CHA0-ΔretS-NiF as an active ingredient. The fluorescent Pseudomonas protegens CHA0-ΔretS-NiF has strong nitrogen-fixing and bactericidal capabilities and may be used to prevent and cure plant diseases and to boost plant growth.
Inventors:
ZHANG YOUMING (CN)
TU QIANG (CN)
YU FANGNAN (CN)
JING XIAOSHU (CN)
BIAN XIAOYING (CN)
CHEN HANNA (CN)
TU QIANG (CN)
YU FANGNAN (CN)
JING XIAOSHU (CN)
BIAN XIAOYING (CN)
CHEN HANNA (CN)
Application Number:
PCT/CN2018/103224
Publication Date:
May 02, 2019
Filing Date:
August 30, 2018
Export Citation:
Assignee:
UNIV SHANDONG (CN)
DEZHOU MICROP BIO TECH CO LTD (CN)
DEZHOU MICROP BIO TECH CO LTD (CN)
International Classes:
C12N1/20; A01N63/27; A01P1/00; A01P3/00; A01P7/04; A01P21/00; C12R1/39
Foreign References:
| CN107557326A | 2018-01-09 | |||
| CN107119000A | 2017-09-01 |
Other References:
LI, J. ET AL: "Bookmark and Share Email this article Print this article Effect of retS gene on biosynthesis of 2,4-diacetyl-phloroglucinol in Pseudomonas fluorescens 2P24", ACTA MICROBIOLOGICA SINICA, vol. 53, no. 2, 4 February 2013 (2013-02-04), pages 118 - 126, ISSN: 0001-6209
LORENA SETTEN: "Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient Conditions", PLOS ONE, vol. 8, no. 5, 13 May 2013 (2013-05-13), pages e63666, XP055595000, ISSN: 1932-6203
ALEXANDRE JOUSSET: "Full-Genome Sequence of the Plant Growth-Promoting Ba- cterium Pseudomonas protegens CHAO", GENOME ANNOUNCEMENTS, vol. 2, no. 2, 24 April 2014 (2014-04-24), XP055595002, ISSN: 2169-8287
LORENA SETTEN: "Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient Conditions", PLOS ONE, vol. 8, no. 5, 13 May 2013 (2013-05-13), pages e63666, XP055595000, ISSN: 1932-6203
ALEXANDRE JOUSSET: "Full-Genome Sequence of the Plant Growth-Promoting Ba- cterium Pseudomonas protegens CHAO", GENOME ANNOUNCEMENTS, vol. 2, no. 2, 24 April 2014 (2014-04-24), XP055595002, ISSN: 2169-8287
Attorney, Agent or Firm:
CHINA PATENT AGENT (H.K.) LTD. (CN)
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