Title:
BACTERIOLYSIS METHOD AND BACTERIA DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/095845
Kind Code:
A1
Abstract:
Provided is a new bacteriolysis method capable of bacteriolysing multiple species of bacteria and making bacteriolysis treatment more efficient and faster. A bacteriolysis method for lysing multispecies bacterial groups in a specimen, wherein bacteriolysis of multispecies bacterial groups is made possible by a step that reacts a specimen with a surfactant and lytic enzyme in a liquid and bacteriolyses the multispecies bacterial groups present in the specimen.
Inventors:
TAKAHASHI KATSUYOSHI (JP)
FUKUSHIMA MASAKAZU (JP)
FUKUSHIMA MASAKAZU (JP)
Application Number:
PCT/JP2022/043413
Publication Date:
June 01, 2023
Filing Date:
November 24, 2022
Export Citation:
Assignee:
ASAHI CHEMICAL IND (JP)
International Classes:
C12N1/06; C07K16/12; C12M1/33; C12M1/34; C12N1/20; C12N9/52; C12Q1/04; C12Q1/37; G01N33/48; G01N33/543; G01N33/569; C12N15/13
Domestic Patent References:
| WO2015093544A1 | 2015-06-25 | |||
| WO2015093545A1 | 2015-06-25 | |||
| WO2015093544A1 | 2015-06-25 | |||
| WO2015093545A1 | 2015-06-25 | |||
| WO2000006603A1 | 2000-02-10 | |||
| WO2000006603A1 | 2000-02-10 |
Foreign References:
| JP2019508019A | 2019-03-28 | |||
| JPH10120539A | 1998-05-12 | |||
| JP2021156799A | 2021-10-07 | |||
| CN112522370A | 2021-03-19 | |||
| JP2017032579A | 2017-02-09 |
Other References:
KIM YOUNG-BUM; PARK JI-HO; CHANG WOO-JIN; KOO YOON-MO; KIM EUN-KI; KIM JIN-HWAN: "Statistical optimization of the lysis agents for Gram-negative bacterial cells in a microfluidic device", BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, KOREAN SOCIETY FOR BIOTECHNOLOGY AND BIOENGINEERING, SEOUL, KR, vol. 11, no. 4, 1 January 1901 (1901-01-01), KR , pages 288 - 292, XP036031543, ISSN: 1226-8372, DOI: 10.1007/BF03026242
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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