Title:
BACTERIUM PRODUCING MONOPHOSPHORYL LIPID A AND METHOD OF PRODUCING MONOPHOSPHORYL LIPID A BY USING BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2017/119628
Kind Code:
A1
Abstract:
A bacterium producing monophosphoryl lipid A (MLA) comprising a genetic modification that increases expression of a gene encoding LpxE polypeptide and a method of producing MLA are provided. According to the present invention, MLA may be produced in a simple manner without acid hydrolysis and/or base hydrolysis.
Inventors:
CHUNG HAK SUK (KR)
YANG EUN GYEONG (KR)
HWANG DOHYEON (KR)
YANG EUN GYEONG (KR)
HWANG DOHYEON (KR)
Application Number:
PCT/KR2016/014761
Publication Date:
July 13, 2017
Filing Date:
December 16, 2016
Export Citation:
Assignee:
KOREA INST SCI & TECH (KR)
International Classes:
C12N15/63; C12N9/10; C12N9/18; C12P7/64; C12P19/04
Foreign References:
| US20130230555A1 | 2013-09-05 | |||
| US20100272758A1 | 2010-10-28 | |||
| KR20160028870A | 2016-03-14 | |||
| CN104844665A | 2015-08-19 | |||
| CN102399736A | 2012-04-04 |
Other References:
HAN ET AL.: "Construction of monophosphoryl lipid A producing Escherichia coli mutants and comparison of immuno-stimulatory activities of their lipopolysaccharides", MARINE DRUGS, vol. 11, 2013, pages 363 - 376, XP 055397358
GILLESPIE ET AL.: "Discovery and purification of AaLpxE, a bi-functional lipid phosphatase from Aquifex aeolicus", THE FASEB JOURNAL, vol. 26, no. 1, 2012, pages 787.4, XP009511501
RAETZ ET AL.: "Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4", JOURNAL OF LIPID RESEARCH, vol. 47, no. 5, 2006, pages 1097 - 1111, XP 002651695
BIWEN WANG ET AL.: "Immuno-Stimulatory Activity of Escherichia coli Mutants Producing Kdo2-Monophosphoryl-Lipid A or Kdo2-Pentaacyl- Monophosphoryl -Lipid A", PLOS ONE, vol. 10, no. 12, 2015, XP055577901, DOI: 10.1371/journal.pone.0144714
YANING HAN ET AL.: "Construction of Monophosphoryl Lipid A Producing Escherichia coli Mutants and Comparison of Immuno-Stimulatory Activities of Their Lipopolysaccharides", MARINE DRUGS, vol. 11, no. 2, 2013, XP055397358, DOI: 10.3390/md11020363
BLIGH, E.G.DYER, W.J., CAN. J. BIOCHEM. PHYSIOL., vol. 37, 1959, pages 911 - 917
"GenBank", Database accession no. NP_214169.1
WANG, R. F.KUSHNER, S. R., GENE, vol. 100, 1991, pages 195 - 199
CHUNG, H. S.RAETZ, C. R., BIOCHEMISTRY, vol. 49, no. 19, 2010, pages 4126 - 4137
KIRILL A. DATSENKOBARRY L. WANNER, PNAS, vol. 97, 2000, pages 6640 - 6645
WANG, X.KARBARZ, M. J.MCGRATH, S. C.COTTER, R. J.RAETZ, C. R., J BIOL CHEM, vol. 279, no. 47, 2004, pages 49470 - 49478
See also references of EP 3400303A4
GILLESPIE ET AL.: "Discovery and purification of AaLpxE, a bi-functional lipid phosphatase from Aquifex aeolicus", THE FASEB JOURNAL, vol. 26, no. 1, 2012, pages 787.4, XP009511501
RAETZ ET AL.: "Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4", JOURNAL OF LIPID RESEARCH, vol. 47, no. 5, 2006, pages 1097 - 1111, XP 002651695
BIWEN WANG ET AL.: "Immuno-Stimulatory Activity of Escherichia coli Mutants Producing Kdo2-Monophosphoryl-Lipid A or Kdo2-Pentaacyl- Monophosphoryl -Lipid A", PLOS ONE, vol. 10, no. 12, 2015, XP055577901, DOI: 10.1371/journal.pone.0144714
YANING HAN ET AL.: "Construction of Monophosphoryl Lipid A Producing Escherichia coli Mutants and Comparison of Immuno-Stimulatory Activities of Their Lipopolysaccharides", MARINE DRUGS, vol. 11, no. 2, 2013, XP055397358, DOI: 10.3390/md11020363
BLIGH, E.G.DYER, W.J., CAN. J. BIOCHEM. PHYSIOL., vol. 37, 1959, pages 911 - 917
"GenBank", Database accession no. NP_214169.1
WANG, R. F.KUSHNER, S. R., GENE, vol. 100, 1991, pages 195 - 199
CHUNG, H. S.RAETZ, C. R., BIOCHEMISTRY, vol. 49, no. 19, 2010, pages 4126 - 4137
KIRILL A. DATSENKOBARRY L. WANNER, PNAS, vol. 97, 2000, pages 6640 - 6645
WANG, X.KARBARZ, M. J.MCGRATH, S. C.COTTER, R. J.RAETZ, C. R., J BIOL CHEM, vol. 279, no. 47, 2004, pages 49470 - 49478
See also references of EP 3400303A4
Attorney, Agent or Firm:
Y.P.LEE, MOCK & PARTNERS (KR)
Download PDF: