BASE CALLING METHOD AND SYSTEM, GENE SEQUENCER AND STORAGE MEDIUM

WIPO Patent Application WO/2024/000312

A1

A base calling method and system, a gene sequencer and a storage medium. The base calling method comprises the following steps: acquiring a first image of a biochip in a red light channel and a second image of the biochip in a green light channel (S1); performing base grouping according to the first image and the second image, and preliminarily identifying the base category of each group (S2); when the number of the base categories of all the groups is at least two, adjusting the brightness value of the first image and the brightness value of the second image according to the base categories of all the groups (S3); respectively performing normalization processing on the first image and the second image (S4); and performing base grouping according to the normalized first image and the normalized second image, and identifying the base category of each group again (S5). The base calling method can accurately identify base categories for data to be sequenced in which some base categories are missing, so that the accuracy of gene sequencing can be improved.

LI MEI (CN)
LI YUXIANG (CN)
ZHANG WENWEI (CN)
XU XUN (CN)

PCT/CN2022/102503

January 04, 2024

June 29, 2022

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BGI SHENZHEN (CN)

G01N21/64; C12Q1/6869; G06T7/00

CN106770114A	2017-05-31
CN111041076A	2020-04-21
CN109117796A	2019-01-01
CN113012757A	2021-06-22
CN102449167A	2012-05-09
EP0648844A2	1995-04-19
US6136543A	2000-10-24

ERHUI JIA, LI XIAO; ZHANG TAO; LI BIN; ZHAO LIHUA : "Peak matching in DNA base-calling based on dynamic programming ", ANALYTICAL INSTRUMENTATION, vol. 2, 28 March 2020 (2020-03-28), pages 70 - 76, XP093121900

SHANGHAI BESHINING LAW OFFICE (CN)

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